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ECEL1 (NM_004826) Human Tagged ORF Clone
CAT＃: RC208405
ECEL1 (Myc-DDK-tagged)-Human endothelin converting enzyme-like 1 (ECEL1)
ORF Plasmid: tGFP
"NM_004826" in other vectors (4)
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CNY 8,512.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DA5D; DINE; ECEX; XCE |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC208405 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCCCCGTATTCGCTGACGGCGCACTACGATGAGTTCCAAGAGGTCAAGTACGTGAGCCGCTGCG GCGCGGGGGGCGCGCGCGGGGCCTCCCTGCCCCCGGGCTTCCCGTTGGGCGCTGCGCGCAGCGCCACCGG GGCCCGGTCCGGGCTGCCGCGCTGGAACCGGCGCGAGGTGTGCCTGCTGTCGGGGCTGGTGTTCGCCGCC GGCCTCTGCGCCATTCTGGCGGCTATGCTGGCCCTCAAGTACCTGGGCCCGGTCGCGGCCGGCGGCGGCG CCTGTCCCGAGGGCTGCCCTGAGCGCAAGGCCTTCGCGCGCGCCGCTCGCTTCCTGGCCGCCAACCTGGA CGCCAGCATCGACCCATGCCAGGACTTCTACTCGTTCGCCTGCGGCGGTTGGCTGCGGCGCCACGCCATC CCCGACGACAAGCTCACCTATGGCACCATCGCGGCCATCGGCGAGCAAAACGAGGAGCGCCTACGGCGCC TGCTGGCGCGGCCCGGGGGTGGGCCTGGCGGCGCGGCCCAGCGCAAGGTGCGCGCCTTCTTCCGCTCGTG CCTCGACATGCGCGAGATCGAGCGACTGGGCCCGCGACCCATGCTAGAGGTCATCGAGGACTGCGGGGGC TGGGACCTGGGCGGCGCGGAGGAGCGTCCGGGGGTCGCGGCGCGATGGGACCTCAACCGGCTGCTGTACA AGGCGCAGGGCGTGTACAGCGCCGCCGCGCTCTTCTCGCTCACGGTCAGCCTGGACGACAGGAACTCCTC GCGCTACGTCATCCGCATTGACCAGGATGGGCTCACCCTGCCAGAGAGGACCCTGTACCTCGCTCAGGAT GAGGACAGTGAGAAGATCCTGGCAGCATACAGGGTGTTCATGGAGCGAGTGCTCAGCCTCCTGGGTGCAG ACGCTGTGGAACAGAAGGCCCAAGAGATCCTGCAAGTGGAGCAGCAGCTGGCCAACATCACTGTGTCAGA GTATGACGACCTACGGCGAGATGTCAGCTCCATGTACAACAAGGTGACGCTGGGGCAGCTGCAGAAGATC ACCCCCCACTTGCGGTGGAAGTGGCTGCTAGACCAGATCTTCCAGGAGGACTTCTCAGAGGAAGAGGAGG TGGTGCTGCTGGCGACAGACTACATGCAGCAGGTGTCGCAGCTCATCCGCTCCACACCCCACCGGGTCCT GCACAACTACCTGGTGTGGCGCGTGGTGGTGGTCCTGAGTGAACACCTGTCCCCGCCATTCCGTGAGGCA CTGCACGAGCTGGCACAGGAGATGGAGGGCAGCGACAAGCCACAGGAGCTGGCCCGGGTCTGCTTGGGCC AGGCCAATCGCCACTTTGGCATGGCGCTTGGCGCCCTCTTTGTACATGAGCACTTCTCAGCTGCCAGCAA AGCCAAGGTGCAGCAGCTAGTGGAAGACATCAAGTACATCCTGGGCCAGCGCCTGGAGGAGCTGGACTGG ATGGACGCCGAGACCAGGGCTGCTGCTCGGGCCAAGCTCCAGTACATGATGGTGATGGTCGGCTACCCGG ACTTCCTGCTGAAACCCGATGCTGTGGACAAGGAGTATGAGTTTGAGGTCCATGAGAAGACCTACTTCAA GAACATCTTGAACAGCATCCGCTTCAGCATCCAGCTCTCAGTTAAGAAGATTCGGCAGGAGGTGGACAAG TCCACGTGGCTGCTCCCCCCACAGGCGCTCAATGCCTACTATCTACCCAACAAGAACCAGATGGTGTTCC CCGCGGGCATCCTGCAGCCCACCCTGTACGACCCTGACTTCCCACAGTCTCTCAACTACGGGGGCATCGG CACCATCATTGGACATGAGCTGACCCACGGCTACGACGACTGGGGGGGCCAGTATGACCGCTCAGGGAAC CTGCTGCACTGGTGGACGGAGGCCTCCTACAGCCGCTTCCTGCGAAAGGCTGAGTGCATCGTCCGTCTCT ATGACAACTTCACTGTCTACAACCAGCGGGTGAACGGGAAACACACGCTTGGGGAGAACATCGCAGATAT GGGCGGCCTCAAGCTGGCCTACCACGCCTATCAGAAGTGGGTGCGGGAGCACGGCCCAGAGCACCCACTT CCCCGGCTCAAGTACACACATGACCAGCTCTTCTTCATTGCCTTTGCCCAGAACTGGTGCATCAAGCGGC GGTCGCAGTCCATCTACCTGCAGGTGCTGACTGACAAGCATGCCCCTGAGCACTACAGGGTGCTGGGCAG TGTGTCCCAGTTTGAGGAGTTTGGCCGGGCTTTCCACTGTCCCAAGGACTCACCCATGAACCCTGCCCAC AAGTGTTCCGTGTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208405 protein sequence
Red=Cloning site Green=Tags(s) MEPPYSLTAHYDEFQEVKYVSRCGAGGARGASLPPGFPLGAARSATGARSGLPRWNRREVCLLSGLVFAA GLCAILAAMLALKYLGPVAAGGGACPEGCPERKAFARAARFLAANLDASIDPCQDFYSFACGGWLRRHAI PDDKLTYGTIAAIGEQNEERLRRLLARPGGGPGGAAQRKVRAFFRSCLDMREIERLGPRPMLEVIEDCGG WDLGGAEERPGVAARWDLNRLLYKAQGVYSAAALFSLTVSLDDRNSSRYVIRIDQDGLTLPERTLYLAQD EDSEKILAAYRVFMERVLSLLGADAVEQKAQEILQVEQQLANITVSEYDDLRRDVSSMYNKVTLGQLQKI TPHLRWKWLLDQIFQEDFSEEEEVVLLATDYMQQVSQLIRSTPHRVLHNYLVWRVVVVLSEHLSPPFREA LHELAQEMEGSDKPQELARVCLGQANRHFGMALGALFVHEHFSAASKAKVQQLVEDIKYILGQRLEELDW MDAETRAAARAKLQYMMVMVGYPDFLLKPDAVDKEYEFEVHEKTYFKNILNSIRFSIQLSVKKIRQEVDK STWLLPPQALNAYYLPNKNQMVFPAGILQPTLYDPDFPQSLNYGGIGTIIGHELTHGYDDWGGQYDRSGN LLHWWTEASYSRFLRKAECIVRLYDNFTVYNQRVNGKHTLGENIADMGGLKLAYHAYQKWVREHGPEHPL PRLKYTHDQLFFIAFAQNWCIKRRSQSIYLQVLTDKHAPEHYRVLGSVSQFEEFGRAFHCPKDSPMNPAH KCSVW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004826 |
| ORF Size | 2325 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004826.4 |
| RefSeq Size | 2909 bp |
| RefSeq ORF | 2328 bp |
| Locus ID | 9427 |
| UniProt ID | O95672 |
| Protein Families | Druggable Genome, Protease, Transmembrane |
| MW | 87.8 kDa |
| Gene Summary | This gene encodes a member of the M13 family of endopeptidases. Members of this family are zinc-containing type II integral-membrane proteins that are important regulators of neuropeptide and peptide hormone activity. Mutations in this gene are associated with autosomal recessive distal arthrogryposis, type 5D. This gene has multiple pseudogenes on chromosome 2. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208405L3 | Lenti ORF clone of Human endothelin converting enzyme-like 1 (ECEL1), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC208405L4 | Lenti ORF clone of Human endothelin converting enzyme-like 1 (ECEL1), mGFP tagged |
CNY 7,700.00 |
|
| RG208405 | ECEL1 (tGFP-tagged) - Human endothelin converting enzyme-like 1 (ECEL1) |
CNY 10,112.00 |
|
| SC125561 | ECEL1 (untagged)-Human endothelin converting enzyme-like 1 (ECEL1) |
CNY 8,528.00 |
