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ADAM18 (NM_014237) Human Tagged ORF Clone
CAT＃: RC211531
ADAM18 (Myc-DDK-tagged)-Human ADAM metallopeptidase domain 18 (ADAM18), transcript variant 1
ORF Plasmid: tGFP
"NM_014237" in other vectors (4)
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CNY 5,416.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ADAM27; tMDCIII |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC211531 representing NM_014237
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCCTTCTCCTCGCCCTCCTCACTGAGCTTGGAAGACTGCAAGCCCACGAAGGTTCTGAAGGAATAT TTCTGCATGTCACAGTTCCACGGAAGATTAAGTCAAATGACAGTGAAGTTTCAGAGAGGAAGATGATTTA CATCATTACAATTGATGGACAACCTTACACTCTACATCTCGGAAAACAATCATTCTTACCCCAGAACTTT TTGGTTTATACATATAATGAAACTGGATCTTTGCATTCTGTGTCTCCATATTTTATGATGCATTGCCATT ACCAAGGATATGCTGCCGAATTTCCAAATTCATTTGTGACACTCAGTATATGTTCTGGTCTCAGGGGATT TCTCCAGTTTGAAAATATCAGTTATGGAATTGAACCAGTAGAATCTTCAGCAAGATTTGAGCATATAATT TATCAAATGAAAAATAATGATCCAAATGTATCCATTTTAGCAGTAAATTACAGTCATATTTGGCAGAAAG ACCAGCCCTACAAAGTTCCTTTAAACTCACAGATAAAAAATCTTTCAAAACTATTACCCCAATATCTGGA AATATACATTATAGTGGAAAAAGCTTTGTATGATTATATGGGATCTGAAATGATGGCTGTAACACAAAAA ATTGTCCAGGTTATTGGGCTTGTCAACACTATGTTTACCCAGTTCAAATTGACTGTTATACTGTCTTCCT TGGAATTGTGGTCAAATGAAAACCAGATTTCCACCAGTGGGGATGCTGATGATATATTACAAAGATTTTT GGCATGGAAACGGGACTATCTCATCCTACGGCCCCATGACATAGCATACTTACTTGTTTACAGGAAACAT CCTAAATATGTGGGAGCAACATTTCCTGGCACTGTATGCAATAAAAGCTATGATGCAGGTATTGCTATGT ATCCAGATGCAATAGGTTTGGAGGGATTTTCGGTTATTATAGCTCAACTGCTTGGCCTTAATGTAGGATT AACATATGATGACATCACTCAGTGTTTCTGTCTGAGAGCTACATGCATCATGAATCATGAAGCAGTGAGT GCCAGTGGTAGAAAGATTTTTAGCAACTGCAGCATGCACGACTATAGATATTTTGTTTCAAAATTTGAGA CTAAATGCCTTCAGAAGCTTTCAAATTTGCAACCATTACATCAAAATCAACCAGTGTGTGGTAATGGGAT TTTGGAATCCAATGAAGAATGTGACTGTGGTAATAAAAATGAATGTCAATTTAAGAAGTGCTGTGATTAT AACACATGTAAACTGAAGGGCTCAGTAAAATGTGGTTCTGGACCATGTTGTACATCAAAGTGTGAGTTGT CAATAGCAGGCACTCCATGTAGAAAGAGTATTGATCCAGAGTGTGATTTTACAGAGTACTGCAATGGAAC CTCTAGTAATTGTGTTCCTGACACTTATGCATTGAATGGCCGTTTGTGCAAGTTGGGAACTGCCTATTGC TATAACGGACAATGTCAAACTACTGATAACCAGTGTGCCAAGATATTTGGAAAAGGTGCTCAAGGTGCTC CATTTGCCTGTTTTAAAGAAGTTAATTCTCTGCATGAAAGATCTGAAAACTGTGGTTTTAAAAATTCACA ACCATTACCTTGTGAACGGAAGGATGTTCTCTGTGGAAAATTAGCTTGTGTTCAGCCACATAAAAATGCT AATAAAAGTGACGCTCAATCTACAGTTTATTCATATATTCAAGACCATGTATGTGTATCTATAGCCACTG GTTCCTCCATGAGATCAGATGGAACAGACAATGCCTATGTGGCTGATGGCACCATGTGTGGTCCAGAAAT GTACTGTGTAAATAAAACCTGCAGAAAAGTTCATTTAATGGGATATAACTGTAATGCCACCACAAAATGC AAAGGGAAAGGGATATGTAATAATTTTGGTAATTGTCAATGCTTCCCTGGACATAGACCTCCAGATTGTA AATTCCAGTTTGGTTCCCCAGGGGGTAGTATTGATGATGGAAATTTTCAGAAATCTGGTGACTTTTATAC TGAAAAAGGCTACAATACACACTGGAACAACTGGTTTATTCTGAGTTTCTGCATTTTTCTGCCGTTTTTC ATAGTTTTCACCACTGTGATCTTTAAAAGAAATGAAATAAGTAAATCATGTAACAGAGAGAATGCAGAGT ATAATCGTAATTCATCCGTTGTATCAGAAAGCGATGACGTGGGACAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC211531 representing NM_014237
Red=Cloning site Green=Tags(s) MFLLLALLTELGRLQAHEGSEGIFLHVTVPRKIKSNDSEVSERKMIYIITIDGQPYTLHLGKQSFLPQNF LVYTYNETGSLHSVSPYFMMHCHYQGYAAEFPNSFVTLSICSGLRGFLQFENISYGIEPVESSARFEHII YQMKNNDPNVSILAVNYSHIWQKDQPYKVPLNSQIKNLSKLLPQYLEIYIIVEKALYDYMGSEMMAVTQK IVQVIGLVNTMFTQFKLTVILSSLELWSNENQISTSGDADDILQRFLAWKRDYLILRPHDIAYLLVYRKH PKYVGATFPGTVCNKSYDAGIAMYPDAIGLEGFSVIIAQLLGLNVGLTYDDITQCFCLRATCIMNHEAVS ASGRKIFSNCSMHDYRYFVSKFETKCLQKLSNLQPLHQNQPVCGNGILESNEECDCGNKNECQFKKCCDY NTCKLKGSVKCGSGPCCTSKCELSIAGTPCRKSIDPECDFTEYCNGTSSNCVPDTYALNGRLCKLGTAYC YNGQCQTTDNQCAKIFGKGAQGAPFACFKEVNSLHERSENCGFKNSQPLPCERKDVLCGKLACVQPHKNA NKSDAQSTVYSYIQDHVCVSIATGSSMRSDGTDNAYVADGTMCGPEMYCVNKTCRKVHLMGYNCNATTKC KGKGICNNFGNCQCFPGHRPPDCKFQFGSPGGSIDDGNFQKSGDFYTEKGYNTHWNNWFILSFCIFLPFF IVFTTVIFKRNEISKSCNRENAEYNRNSSVVSESDDVGH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014237 |
| ORF Size | 2217 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014237.3 |
| RefSeq Size | 2248 bp |
| RefSeq ORF | 2220 bp |
| Locus ID | 8749 |
| UniProt ID | Q9Y3Q7 |
| Protein Families | Druggable Genome, Protease, Transmembrane |
| MW | 82.8 kDa |
| Gene Summary | This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The encoded preproprotein is proteolytically processed to generate the mature sperm surface protein. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211531L3 | Lenti-ORF clone of ADAM18 (Myc-DDK-tagged)-Human ADAM metallopeptidase domain 18 (ADAM18), transcript variant 1 |
CNY 7,700.00 |
|
| RC211531L4 | Lenti-ORF clone of ADAM18 (mGFP-tagged)-Human ADAM metallopeptidase domain 18 (ADAM18), transcript variant 1 |
CNY 7,700.00 |
|
| RG211531 | ADAM18 (tGFP-tagged) - Human ADAM metallopeptidase domain 18 (ADAM18), transcript variant 1 |
CNY 6,370.00 |
|
| SC125245 | ADAM18 (untagged)-Human ADAM metallopeptidase domain 18 (ADAM18), transcript variant 1 |
CNY 14,160.00 |
