EGFLAM (NM_182801) Human Tagged ORF Clone

CAT#: RC212516

  • TrueORF®

EGFLAM (Myc-DDK-tagged)-Human EGF-like, fibronectin type III and laminin G domains (EGFLAM), transcript variant 4

ORF Plasmid: DDK tGFP



  "NM_182801" in other vectors (6)

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CNY 1,200.00

CNY 3,705.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal Anti-EGFLAM Antibody
    • 50 ug

CNY 5,200.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms AGRINL; AGRNL; PIKA
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC212516 representing NM_182801
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGTTTAAAACAACTGCCAAGGATGGCCTTTTGCTGTGGAGGGGAGACAGCCCCATGAGACCCAACA
GCGACTTCATTTCCTTGGGCCTTCGGGATGGAGCCCTCGTGTTCAGCTATAACCTGGGCAGTGGTGTGGC
ATCCATCATGGTGAATGGCTCCTTCAACGATGGTCGGTGGCACCGAGTTAAGGCCGTTAGGGATGGCCAG
TCAGGAAAGATAACCGTGGATGACTATGGAGCCAGAACAGGCAAATCCCCAGGCATGATGCGGCAGCTTA
ACATCAATGGAGCTCTGTATGTGGGTGGAATGAAGGAAATTGCTCTGCACACTAACAGGCAATATATGAG
AGGGCTCGTGGGCTGTATCTCTCACTTCACCCTGTCCACCGATTACCACATTTCCCTCGTGGAAGATGCC
GTGGATGGAAAAAACATCAACACTTGTGGAGCCAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC212516 representing NM_182801
Red=Cloning site Green=Tags(s)

MRFKTTAKDGLLLWRGDSPMRPNSDFISLGLRDGALVFSYNLGSGVASIMVNGSFNDGRWHRVKAVRDGQ
SGKITVDDYGARTGKSPGMMRQLNINGALYVGGMKEIALHTNRQYMRGLVGCISHFTLSTDYHISLVEDA
VDGKNINTCGAK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_182801
ORF Size 456 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_182801.3
RefSeq Size 2228 bp
RefSeq ORF 459 bp
Locus ID 133584
UniProt ID Q63HQ2
Protein Families Transmembrane
MW 16.4 kDa
Gene Summary Involved in both the retinal photoreceptor ribbon synapse formation and physiological functions of visual perception. Necessary for proper bipolar dendritic tip apposition to the photoreceptor ribbon synapse. Promotes matrix assembly and cell adhesiveness (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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