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CRNKL1 (NM_016652) Human Tagged ORF Clone
CAT＃: RC220334
CRNKL1 (Myc-DDK-tagged)-Human crooked neck pre-mRNA splicing factor-like 1 (Drosophila) (CRNKL1)
ORF Plasmid: tGFP
"NM_016652" in other vectors (4)
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CNY 6,208.00
CNY 7,130.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CLF; Clf1; CRN; HCRN; MSTP021; SYF3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC220334 representing NM_016652
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGGCAACAGTGGAGAACCTAACGTTCCAGAAGGACACTTTGGGAAATGCTGTTGACAAAAACACAT CCAGATTGGAGCTCCGTTCTTACTCCCTTGCAGGGCGCCACGGCTCGACAGAGCCACTCGTCCTCGCCTG GTCATCCCAGTTCCGGAGGCTGACCTGGGGATGCGCCCTGGACGCCCTGCACCGCAGTCCTTGCGTCGCT GCTAGTCAGCACGGCGTTACCCACCTCATCCGCAGCTCCAGGACGCCGCACTCCACGCGCTGCCGCAAGG AGGACGCGCAGCCTGGTCACCATGGAAACGGTGCCGCCTCCGTCACGGCTCAAGCGAGAGGTCAAAGGTC AGTCCTGCAAGTTCCGCTTCCGGTTCCGAGATCCTGTTTGTTTTCTGAAAGCTTTGTGGTTTCGGTGAGC TCTCAGAGCCGATTTCTAGCGTCCGTGCCGGGGACAGGTGTCCAGAGGTCGACTGCTGCAGACATGGCGG CCTCCACCGCGGCCGGGAAGCAGCGGATTCCCAAAGTGGCCAAGGTGAAAAACAAAGCCCCGGCTGAGGT ACAGATAACTGCTGAACAACTCTTAAGAGAGGCTAAAGAAAGAGAACTTGAGCTTCTTCCACCTCCACCT CAACAGAAGATCACAGATGAAGAAGAATTAAATGATTATAAACTAAGGAAAAGGAAGACTTTTGAAGATA ATATAAGAAAAAACAGGACTGTGATTAGTAACTGGATAAAATACGCACAATGGGAAGAAAGCCTAAAGGA GATTCAAAGGGCTCGATCCATATACGAGCGTGCTTTAGATGTAGACTACCGAAATATTACACTCTGGCTG AAATACGCAGAAATGGAAATGAAGAATCGCCAAGTCAACCATGCTCGAAATATCTGGGACCGGGCCATAA CAACGCTGCCTCGAGTTAATCAGTTCTGGTACAAGTACACGTACATGGAGGAAATGTTGGGAAACGTTGC CGGTGCCCGGCAGGTGTTTGAGCGCTGGATGGAGTGGCAGCCTGAGGAGCAAGCCTGGCACTCCTACATC AACTTTGAGCTGAGATACAAAGAGGTGGATCGGGCCCGCACCATTTATGAGCGATTTGTCCTCGTGCACC CTGATGTTAAGAACTGGATCAAGTATGCCCGCTTTGAAGAAAAACATGCTTATTTTGCCCATGCACGGAA AGTGTATGAGAGAGCTGTGGAATTCTTTGGAGATGAACATATGGATGAGCACCTTTATGTTGCCTTTGCC AAGTTTGAAGAAAATCAGAAAGAGTTTGAAAGGGTACGAGTGATTTACAAGTATGCCCTGGACAGAATTT CAAAACAAGATGCCCAAGAACTCTTTAAAAATTATACCATCTTTGAGAAGAAGTTTGGTGATAGGCGGGG TATTGAAGATATCATTGTGAGCAAACGGAGATTCCAGTACGAAGAAGAAGTGAAGGCGAATCCACACAAT TATGATGCATGGTTTGATTACTTGCGCTTGGTAGAAAGTGACGCAGAAGCTGAAGCCGTGAGAGAAGTCT ATGAAAGGGCCATTGCCAATGTCCCACCCATTCAGGAGAAGAGGCACTGGAAGCGCTACATTTATCTTTG GATCAACTATGCACTCTATGAAGAATTGGAGGCAAAGGATCCTGAGAGGACAAGACAGGTGTATCAAGCC TCTTTGGAACTAATTCCTCACAAAAAGTTCACATTTGCCAAAATGTGGATACTGTATGCACAGTTTGAAA TACGACAGAAGAATCTGTCATTAGCCAGAAGAGCATTGGGAACTTCCATAGGCAAATGTCCAAAGAACAA ATTATTTAAAGTTTACATAGAATTGGAGCTACAGCTTCGAGAATTTGACAGATGCCGGAAGCTTTATGAA AAGTTCCTGGAATTTGGACCTGAAAATTGTACCTCATGGATTAAATTCGCTGAATTAGAGACAATCCTTG GTGATATTGACAGAGCACGGGCAATCTATGAATTAGCCATCAGTCAGCCACGTTTAGACATGCCAGAGGT GCTTTGGAAATCATATATTGATTTTGAAATTGAGCAGGAAGAAACAGAAAGAACACGAAACCTTTACCGG CGGTTGCTTCAACGGACGCAGCATGTCAAGGTATGGATCAGCTTTGCTCAGTTTGAGTTGTCTTCAGGAA AAGAAGGAAGTTTGACTAAATGCAGACAAATTTATGAAGAAGCTAACAAAACCATGCGAAACTGTGAAGA AAAGGAAGAGAGACTTATGCTGCTGGAATCTTGGCGAAGTTTTGAAGAAGAATTTGGAACAGCTTCAGAT AAGGAGAGAGTAGACAAACTCATGCCAGAGAAAGTCAAGAAGAGAAGAAAGGTCCAGACTGATGATGGGT CTGATGCAGGCTGGGAAGAATACTTTGATTACATCTTTCCAGAAGATGCTGCCAACCAACCTAACCTCAA ACTCCTGGCCATGGCCAAACTGTGGAAGAAACAGCAGCAGGAAAAGGAGGATGCTGAGCACCATCCAGAT GAGGACGTCGATGAGAGTGAATCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC220334 representing NM_016652
Red=Cloning site Green=Tags(s) MTATVENLTFQKDTLGNAVDKNTSRLELRSYSLAGRHGSTEPLVLAWSSQFRRLTWGCALDALHRSPCVA ASQHGVTHLIRSSRTPHSTRCRKEDAQPGHHGNGAASVTAQARGQRSVLQVPLPVPRSCLFSESFVVSVS SQSRFLASVPGTGVQRSTAADMAASTAAGKQRIPKVAKVKNKAPAEVQITAEQLLREAKERELELLPPPP QQKITDEEELNDYKLRKRKTFEDNIRKNRTVISNWIKYAQWEESLKEIQRARSIYERALDVDYRNITLWL KYAEMEMKNRQVNHARNIWDRAITTLPRVNQFWYKYTYMEEMLGNVAGARQVFERWMEWQPEEQAWHSYI NFELRYKEVDRARTIYERFVLVHPDVKNWIKYARFEEKHAYFAHARKVYERAVEFFGDEHMDEHLYVAFA KFEENQKEFERVRVIYKYALDRISKQDAQELFKNYTIFEKKFGDRRGIEDIIVSKRRFQYEEEVKANPHN YDAWFDYLRLVESDAEAEAVREVYERAIANVPPIQEKRHWKRYIYLWINYALYEELEAKDPERTRQVYQA SLELIPHKKFTFAKMWILYAQFEIRQKNLSLARRALGTSIGKCPKNKLFKVYIELELQLREFDRCRKLYE KFLEFGPENCTSWIKFAELETILGDIDRARAIYELAISQPRLDMPEVLWKSYIDFEIEQEETERTRNLYR RLLQRTQHVKVWISFAQFELSSGKEGSLTKCRQIYEEANKTMRNCEEKEERLMLLESWRSFEEEFGTASD KERVDKLMPEKVKKRRKVQTDDGSDAGWEEYFDYIFPEDAANQPNLKLLAMAKLWKKQQQEKEDAEHHPD EDVDESES TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016652 |
| ORF Size | 2544 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_016652.5, NP_057736.4 |
| RefSeq Size | 4406 bp |
| RefSeq ORF | 2547 bp |
| Locus ID | 51340 |
| UniProt ID | Q9BZJ0 |
| Protein Pathways | Spliceosome |
| MW | 100.3 kDa |
| Gene Summary | The crooked neck (crn) gene of Drosophila is essential for embryogenesis and is thought to be involved in cell cycle progression and pre-mRNA splicing. A protein encoded by this human locus has been found to localize to pre-mRNA splicing complexes in the nucleus and is necessary for pre-mRNA splicing. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2013] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
CRNKL1 Is a Highly Selective Regulator of Intron-Retaining HIV-1 and Cellular mRNAs
,null,
mBio
,PubMed ID 33468685
[CRNKL1]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220334L3 | Lenti ORF clone of Human crooked neck pre-mRNA splicing factor-like 1 (Drosophila) (CRNKL1), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC220334L4 | Lenti ORF clone of Human crooked neck pre-mRNA splicing factor-like 1 (Drosophila) (CRNKL1), mGFP tagged |
CNY 9,030.00 |
|
| RG220334 | CRNKL1 (tGFP-tagged) - Human crooked neck pre-mRNA splicing factor-like 1 (Drosophila) (CRNKL1) |
CNY 7,890.00 |
|
| SC304444 | CRNKL1 (untagged)-Human crooked neck pre-mRNA splicing factor-like 1 (Drosophila) (CRNKL1) |
CNY 16,440.00 |
