RSPO2 (NM_178565) Human Tagged ORF Clone
CAT#: RC224177
RSPO2 (Myc-DDK-tagged)-Human R-spondin 2 homolog (Xenopus laevis) (RSPO2)
ORF Plasmid: tGFP
"NM_178565" in other vectors (7)
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CNY 2,400.00
CNY 3,990.00
Cited in 3 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | CRISTIN2; HHRRD; TETAMS2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC224177 representing NM_178565
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGTTTCGCCTTTTCTCCTTTGCCCTCATCATTCTGAACTGCATGGATTACAGCCACTGCCAAGGCA ACCGATGGAGACGCAGTAAGCGAGCTAGTTATGTATCAAATCCCATTTGCAAGGGTTGTTTGTCTTGTTC AAAGGACAATGGGTGTAGCCGATGTCAACAGAAGTTGTTCTTCTTCCTTCGAAGAGAAGGGATGCGCCAG TATGGAGAGTGCCTGCATTCCTGCCCATCCGGGTACTATGGACACCGAGCCCCAGATATGAACAGATGTG CAAGATGCAGAATAGAAAACTGTGATTCTTGCTTTAGCAAAGACTTTTGTACCAAGTGCAAAGTAGGCTT TTATTTGCATAGAGGCCGTTGCTTTGATGAATGTCCAGATGGTTTTGCACCATTAGAAGAAACCATGGAA TGTGTGGAAGGATGTGAAGTTGGTCATTGGAGCGAATGGGGAACTTGTAGCAGAAATAATCGCACATGTG GATTTAAATGGGGTCTGGAAACCAGAACACGGCAAATTGTTAAAAAGCCAGTGAAAGACACAATACCGTG TCCAACCATTGCTGAATCCAGGAGATGCAAGATGACAATGAGGCATTGTCCAGGAGGGAAGAGAACACCA AAGGCGAAGGAGAAGAGGAACAAGAAAAAGAAAAGGAAGCTGATAGAAAGGGCCCAGGAGCAACACAGCG TCTTCCTAGCTACAGACAGAGCTAACCAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224177 representing NM_178565
Red=Cloning site Green=Tags(s) MQFRLFSFALIILNCMDYSHCQGNRWRRSKRASYVSNPICKGCLSCSKDNGCSRCQQKLFFFLRREGMRQ YGECLHSCPSGYYGHRAPDMNRCARCRIENCDSCFSKDFCTKCKVGFYLHRGRCFDECPDGFAPLEETME CVEGCEVGHWSEWGTCSRNNRTCGFKWGLETRTRQIVKKPVKDTIPCPTIAESRRCKMTMRHCPGGKRTP KAKEKRNKKKKRKLIERAQEQHSVFLATDRANQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_178565 |
ORF Size | 729 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_178565.3, NP_848660.2 |
RefSeq Size | 2842 bp |
RefSeq ORF | 732 bp |
Locus ID | 340419 |
UniProt ID | Q6UXX9 |
Protein Families | Secreted Protein |
MW | 28.1 kDa |
Gene Summary | This gene encodes a member of the R-spondin family of proteins. These proteins are secreted ligands of leucine-rich repeat containing G protein-coupled receptors that enhance Wnt signaling through the inhibition of ubiquitin E3 ligases. A chromosomal translocation including this locus that results in the formation of a gene fusion has been identified in multiple human cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015] |
Citations (3)
The use of this cDNA Clones has been cited in the following citations: |
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Targeting the Wnt signaling pathway through R-spondin 3 identifies an anti-fibrosis treatment strategy for multiple organs
,Zhang, M;Haughey, M;Wang, NY;Blease, K;Kapoun, AM;Couto, S;Belka, I;Hoey, T;Groza, M;Hartke, J;Bennett, B;Cain, J;Gurney, A;Benish, B;Castiglioni, P;Drew, C;Lachowicz, J;Carayannopoulos, L;Nathan, SD;Distler, J;Brenner, DA;Hariharan, K;Cho, H;Xie, W;,
PLoS ONE
,PubMed ID 32160239
[RSPO2]
|
Clinical value of R-spondins in triple-negative and metaplastic breast cancers
,Coussy, F;Lallemand, F;Vacher, S;Schnitzler, A;Chemlali, W;Caly, M;Nicolas, A;Richon, S;Meseure, D;El Botty, R;De-Plater, L;Fuhrmann, L;Dubois, T;Roman-Roman, S;Dangles-Marie, V;Marangoni, E;Bièche, I;,
Br. J. Cancer
,PubMed ID 28472820
[RSPO2]
|
The Role of R-Spondin2 in Keratinocyte Proliferation and Epidermal Thickening in Keloid Scarring
,Alvin W C Chua, Dongrui Ma, Shu U Gan, Zhenying Fu, Hwan C Han, Colin Song, Kanaga Sabapathy, Toan T Phan,
Journal of Investigative Dermatology 131, 644-654 (16 December 2010) doi:10.1038/jid.2010.371 Original Article
[Rspo2]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC224177L1 | Lenti ORF clone of Human R-spondin 2 homolog (Xenopus laevis) (RSPO2), Myc-DDK-tagged |
CNY 4,800.00 |
|
RC224177L2 | Lenti ORF clone of Human R-spondin 2 homolog (Xenopus laevis) (RSPO2), mGFP tagged |
CNY 5,890.00 |
|
RC224177L3 | Lenti ORF clone of Human R-spondin 2 homolog (Xenopus laevis) (RSPO2), Myc-DDK-tagged |
CNY 4,800.00 |
|
RC224177L4 | Lenti ORF clone of Human R-spondin 2 homolog (Xenopus laevis) (RSPO2), mGFP tagged |
CNY 4,800.00 |
|
RG224177 | RSPO2 (tGFP-tagged) - Human R-spondin 2 homolog (Xenopus laevis) (RSPO2) |
CNY 4,370.00 |
|
SC123361 | RSPO2 (untagged)-Human R-spondin 2 homolog (Xenopus laevis) (RSPO2) |
CNY 2,400.00 |
|
SC317347 | RSPO2 (untagged)-Human R-spondin 2 homolog (Xenopus laevis) (RSPO2) |
CNY 2,400.00 |