RDM1 (NM_001163120) Human Tagged ORF Clone

CAT#: RC228100

  • TrueORF®

RDM1 (Myc-DDK-tagged)-Human RAD52 motif 1 (RDM1), transcript variant 3

ORF Plasmid: DDK tGFP



  "NM_001163120" in other vectors (4)

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CNY 3,990.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit polyclonal anti-RDM1 antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms RAD52B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC228100 representing NM_001163120
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGAGTTGGTACCTTTTGCGGTTCCCATCGAGAGTGACAAAACCTTGCTAGTGTGGGAGCTGAGCT
CCGGACCCACGGCCGAGGCTTTGCATCATTCTCTGTTCACAGCATTTTCTCAGTTTGGCCTTCTGTATTC
AGTCCGGGTCTTCCCAAATGCTGCAGTGGCCCATCCTGGTTTCTATGCCGTCATTAAGTTTTATTCTGCA
AGGGCTGCCCACAGAGCCCAAAAGGCATGCGACCGGAAGCAGCTTTTTCAGAAATCTCCAGTCAAGGTTC
GTCTTGGCACCAGACATAAGGCAGTTCAACATCAAGCCCTTGCCCTGAACAGTTCCAAATGCCAAGAACT
GGCGAATTACTACTTTGGTTTCAATGGGTGTTCCAAAAGGATCATCAAGGTCCCTTGCTCTCCCTGGAAG
CAGTATGGCCAAGAGGAGGAAGGGTATCTCTCGGATTTCAGCTTGGAGGAGGAAGAGTTCAGGCTGCCAG
AACTTGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC228100 representing NM_001163120
Red=Cloning site Green=Tags(s)

MAELVPFAVPIESDKTLLVWELSSGPTAEALHHSLFTAFSQFGLLYSVRVFPNAAVAHPGFYAVIKFYSA
RAAHRAQKACDRKQLFQKSPVKVRLGTRHKAVQHQALALNSSKCQELANYYFGFNGCSKRIIKVPCSPWK
QYGQEEEGYLSDFSLEEEEFRLPELD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001163120
ORF Size 498 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001163120.2
RefSeq ORF 501 bp
Locus ID 201299
UniProt ID Q8NG50
MW 18.6 kDa
Gene Summary This gene encodes a protein involved in the cellular response to cisplatin, a drug commonly used in chemotherapy. The protein encoded by this gene contains two motifs: a motif found in RAD52, a protein that functions in DNA double-strand breaks and homologous recombination, and an RNA recognition motif (RRM) that is not found in RAD52. The RAD52 motif region in RAD52 is important for protein function and may be involved in DNA binding or oligomerization. Alternatively spliced transcript variants encoding different isoforms have been reported. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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