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ATP12A (NM_001185085) Human Tagged ORF Clone
CAT＃: RC230888
- TrueORF®
ATP12A (Myc-DDK-tagged)-Human ATPase, H+/K+ transporting, nongastric, alpha polypeptide (ATP12A), transcript variant 1
ORF Plasmid: tGFP
"NM_001185085" in other vectors (3)
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CNY 10,450.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ATP1AL1; H-K-ATPase; HK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC230888 representing NM_001185085
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACCAGAAAACCCCAGAAATTTACTCCGTGGAGCTCAGCGGAACTAAGGACATCGTGAAAACAGACA AGGGGGATGGCAAGGAGAAGTATAGGGGTCTGAAGAACAACTGCCTGGAACTCAAAAAGAAAAATCACAA AGAGGAGTTTCAGAAAGAACTCCATCTGGATGACCACAAACTCAGCAATAGGGAATTGGAAGAGAAATAT GGCACAGACATCATTATGGGTCTCTCCAGCACCAGAGCTGCCGAGCTCCTGGCCCGGGATGGGCCCAACT CCCTCACCCCTCCCAAGCAGACGCCTGAGATCGTCAAGTTCCTCAAGCAGATGGTGGGGGGGTTCTCTAT CCTCCTGTGGGTGGGCGCCTTTCTCTGTTGGATTGCATATGGGATTCAGTACTCCAGCGACAAGTCTGCA TCCCTGAACAACGTGTACTTGGGCTGTGTGCTTGGTCTGGTGGTCATTTTAACGGGGATCTTTGCTTATT ACCAAGAGGCAAAAAGCACCAACATCATGTCCAGCTTCAATAAGATGATCCCTCAGCAAGCTCTCGTCAT CCGAGATTCCGAGAAGAAGACCATCCCTTCAGAGCAGCTGGTGGTGGGGGACATTGTGGAGGTCAAAGGA GGAGACCAGATCCCTGCAGACATCAGGGTGCTGTCTTCTCAGGGGTGTCGGGTGGATAACTCATCTCTCA CGGGGGAGTCTGAGCCCCAGCCCCGCTCCTCTGAGTTTACCCATGAAAACCCCCTGGAAACAAAGAACAT CTGCTTCTATTCCACAACGTGTCTGGAAGCATCTACTTCCCCTGTAGGCACTGTCACCGGCATGGTTATC AACACGGGTGACCGCACCATCATTGGCCATATTGCCTCATTGGCCTCAGGAGTTGGAAATGAGAAGACGC CCATTGCCATTGAGATCGAGCACTTTGTTCACATTGTGGCAGGAGTGGCTGTCTCCATCGGCATCCTTTT CTTCATCATCGCTGTGTCCCTGAAGTATCAAGTCCTGGACTCCATCATCTTCCTCATTGGCATCATTGTG GCCAATGTGCCCGAGGGCCTCCTGGCCACTGTCACTGTGACCCTGTCGCTGACAGCAAAACGGATGGCCA AGAAGAACTGCCTGGTGAAGAACCTGGAGGCTGTGGAGACCCTCGGCTCCACCTCCATCATCTGCTCGGA CAAGACTGGGACACTGACCCAGAACAGGATGACAGTGGCCCATCTGTGGTTCGACAATCAGATCTTTGTG GCTGACACCAGTGAGGACCATTCAAACCAAGTCTTTGACCAAAGCTCTAGGACTTGGGCCTCCTTATCCA AGATAATAACATTGTGTAACCGAGCAGAGTTCAAGCCAGGACAGGAAAATGTCCCCATCATGAAGAAAGC TGTGATTGGAGATGCCTCAGAAACTGCTCTTTTAAAATTCTCAGAGGTCATTTTGGGTGATGTGATGGAA ATTAGAAAAAGAAACCGCAAAGTAGCTGAAATCCCTTTTAACTCTACTAATAAATTTCAGCTCTCCATCC ACGAGATGGATGACCCCCACGGCAAGCGCTTCCTCATGGTGATGAAGGGGGCCCCTGAGCGCATTCTAGA GAAATGCAGCACCATCATGATCAACGGCGAGGAGCACCCACTGGACAAGAGCACTGCCAAGACCTTCCAC ACAGCCTACATGGAGCTGGGCGGGTTGGGCGAGCGTGTGCTGGGTTTCTGTCATCTCTACCTGCCAGCAG ACGAGTTTCCAGAAACCTACTCATTTGACATAGACGCTATGAACTTTCCGACCTCCAACCTCTGTTTTGT GGGACTCTTGTCAATGATCGATCCCCCTCGGTCCACCGTGCCAGATGCAGTCACCAAATGCCGGAGTGCA GGGATCAAGGTTATTATGGTTACTGGTGATCATCCCATCACAGCCAAAGCTATTGCCAAGAGTGTGGGGA TCATTTCAGCCAACAGTGAAACAGTGGAAGACATTGCACATCGCCTCAACATTGCTGTGGAGCAAGTTAA CAAACGGGATGCCAAGGCCGCTGTGGTGACTGGCATGGAGCTGAAGGACATGAGCTCAGAACAGCTGGAT GAGATCTTAGCCAACTACCAGGAGATTGTCTTTGCCCGGACATCCCCCCAGCAGAAGCTGATCATTGTGG AGGGCTGTCAGAGGCAGGATGCTGTTGTTGCTGTGACCGGGGATGGAGTTAATGACTCTCCGGCTCTAAA GAAGGCAGACATTGGGATTGCCATGGGGATAGCAGGTTCTGATGCAGCCAAAAATGCAGCCGACATGGTC TTGCTGGACGACAACTTCGCATCCATCGTCACAGGGGTGGAGGAAGGTCGCCTGATCTTTGACAACCTCA AGAAGACTATTGCTTATTCCCTGACCAAGAACATTGCCGAGCTGTGCCCCTTTCTGATCTACATCATTGT CGGGCTCCCCCTGCCCATTGGCACCATCACCATTCTGTTCATTGACTTGGGGACAGACATTATCCCCTCC ATTGCCTTGGCGTACGAGAAAGCTGAAAGTGACATCATGAACAGGAAGCCTCGCCACAAGAATAAGGACA GGCTGGTGAACCAGCCGCTCGCTGTGTACTCATACCTGCACATTGGCCTCATGCAAGCCCTGGGAGCTTT CCTTGTGTATTTCACCGTCTATGCACAAGAGGGCTTTCTGCCCCGCACTCTCATTAACCTGCGGGTAGAA TGGGAGAAGGACTACGTGAATGACTTGAAAGACAGCTATGGGCAGGAATGGACAAGGTACCAGAGGGAAT ACCTAGAATGGACGGGCTACACGGCTTTCTTTGTTGGCATCCTAGTCCAGCAAATAGCAGATCTGATCAT CAGGAAAACCCGGAGGAATTCCATCTTCCAGCAGGGTCTCTTCAGAAATAAAGTCATCTGGGTGGGGATC ACCTCACAGATCATCATTGGTCTGATCCTCTCCTATGGCCTCGGAAGTGTCACAGCCTTGAGTTTCACCA TGCTTAGGGCTCAGTACTGGTTTGTGGCTGTGCCGCACGCCATCCTGATCTGGGTGTATGATGAGGTGCG GAAGCTCTTCATCAGGCTCTACCCTGGAAGCTGGTGGGATAAGAACATGTATTAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC230888 representing NM_001185085
Red=Cloning site Green=Tags(s) MHQKTPEIYSVELSGTKDIVKTDKGDGKEKYRGLKNNCLELKKKNHKEEFQKELHLDDHKLSNRELEEKY GTDIIMGLSSTRAAELLARDGPNSLTPPKQTPEIVKFLKQMVGGFSILLWVGAFLCWIAYGIQYSSDKSA SLNNVYLGCVLGLVVILTGIFAYYQEAKSTNIMSSFNKMIPQQALVIRDSEKKTIPSEQLVVGDIVEVKG GDQIPADIRVLSSQGCRVDNSSLTGESEPQPRSSEFTHENPLETKNICFYSTTCLEASTSPVGTVTGMVI NTGDRTIIGHIASLASGVGNEKTPIAIEIEHFVHIVAGVAVSIGILFFIIAVSLKYQVLDSIIFLIGIIV ANVPEGLLATVTVTLSLTAKRMAKKNCLVKNLEAVETLGSTSIICSDKTGTLTQNRMTVAHLWFDNQIFV ADTSEDHSNQVFDQSSRTWASLSKIITLCNRAEFKPGQENVPIMKKAVIGDASETALLKFSEVILGDVME IRKRNRKVAEIPFNSTNKFQLSIHEMDDPHGKRFLMVMKGAPERILEKCSTIMINGEEHPLDKSTAKTFH TAYMELGGLGERVLGFCHLYLPADEFPETYSFDIDAMNFPTSNLCFVGLLSMIDPPRSTVPDAVTKCRSA GIKVIMVTGDHPITAKAIAKSVGIISANSETVEDIAHRLNIAVEQVNKRDAKAAVVTGMELKDMSSEQLD EILANYQEIVFARTSPQQKLIIVEGCQRQDAVVAVTGDGVNDSPALKKADIGIAMGIAGSDAAKNAADMV LLDDNFASIVTGVEEGRLIFDNLKKTIAYSLTKNIAELCPFLIYIIVGLPLPIGTITILFIDLGTDIIPS IALAYEKAESDIMNRKPRHKNKDRLVNQPLAVYSYLHIGLMQALGAFLVYFTVYAQEGFLPRTLINLRVE WEKDYVNDLKDSYGQEWTRYQREYLEWTGYTAFFVGILVQQIADLIIRKTRRNSIFQQGLFRNKVIWVGI TSQIIIGLILSYGLGSVTALSFTMLRAQYWFVAVPHAILIWVYDEVRKLFIRLYPGSWWDKNMYY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001185085 |
| ORF Size | 3135 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001185085.1, NP_001172014.1 |
| RefSeq ORF | 3138 bp |
| Locus ID | 479 |
| UniProt ID | P54707 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Oxidative phosphorylation |
| MW | 116.5 kDa |
| Gene Summary | The protein encoded by this gene belongs to the family of P-type cation transport ATPases. This gene encodes a catalytic subunit of the ouabain-sensitive H+/K+ -ATPase that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for potassium absorption in various tissues. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230888L3 | Lenti-ORF clone of ATP12A (Myc-DDK-tagged)-Human ATPase, H+/K+ transporting, nongastric, alpha polypeptide (ATP12A), transcript variant 1 |
CNY 12,350.00 |
|
| RC230888L4 | Lenti-ORF clone of ATP12A (mGFP-tagged)-Human ATPase, H+/K+ transporting, nongastric, alpha polypeptide (ATP12A), transcript variant 1 |
CNY 12,350.00 |
|
| RG230888 | ATP12A (tGFP-tagged) - Human ATPase, H+/K+ transporting, nongastric, alpha polypeptide (ATP12A), transcript variant 1 |
CNY 13,240.00 |
