ARNTL2 (NM_001248004) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	bHLHe6; BMAL2; CLIF; MOP9; PASD9
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234501 representing NM_001248004 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGAAGAGGAGGCTGCGGCGGGAGGTAAAGTGTTGAGAGAGGAGAACCAGTGCATTGCTCCTG TGGTTTCCAGCCGCGTGAGTCCAGGGACAAGACCAACAGCTATGGGGTCTTTCAGCTCACACATGACAGA GTTTCCACGAAAACGCAAAGGAAGTGATTCAGACCCATCCCAAGAAGCTCATAGCCAAACTGAAAAGCGG AGGAGAGATAAAATGAATAACCTGATTGAAGAACTGTCTGCAATGATCCCTCAGTGCAACCCCATGGCGC GTAAACTGGACAAACTTACAGTTTTAAGAATGGCTGTTCAACACTTGAGATCTTTAAAAGGCTTGACAAA TTCTTATGTGGGAAGTAATTATAGACCATCATTTCTTCAGGATAATGAGCTCAGACATTTAATCCTTAAG ACTGCAGAAGGCTTCTTATTTGTGGTTGGATGTGAAAGAGGAAAAATTCTCTTCGTTTCTAAGTCAGTCT CCAAAATACTTAATTATGATCAGGCTAGTTTGACTGGACAAAGCTTATTTGACTTCTTACATCCAAAAGA TGTTGCCAAAGTAAAGGAACAACTTTCTTCTTTTGATATTTCACCAAGAGAAAAGCTAATAGATGCCAAA ACTGGTTTGCAAGTTCACAGTAATCTCCACGCTGGAAGGACACGTGTGTATTCTGGCTCAAGACGATCTT TTTTCTGTCGGATAAAGAGTTGTAAAATCTCTGTCAAAGAAGAGCATGGATGCTTACCCAACTCAAAGAA GAAAGAGCACAGAAAATTCTATACTATCCATTGCACTGGTTACTTGAGAAGCTGGCCTCCAAATATTGTT GGAATGGAAGAAGAAAGGAACAGTAAGAAAGACAACAGTAATTTTACCTGCCTTGTGGCCATTGGAAGAT TACAGCCATATATTGTTCCACAGAACAGTGGAGAGATTAATGTGAAACCAACTGAATTTATAACCCGGTT TGCAGTGAATGGAAAATTTGTCTATGTAGATCAAAGGGCAACAGCGATTTTAGGATATCTGCCTCAGGAA CTTTTGGGAACTTCTTGTTATGAATATTTTCATCAAGATGACCACAATAATTTGACTGACAAGCACAAAG CAGTTCTACAGAGTAAGGAGAAAATACTTACAGATTCCTACAAATTCAGAGCAAAAGATGGCTCTTTTGT AACTTTAAAAAGCCAATGGTTTAGTTTCACAAATCCTTGGACAAAAGAACTGGAATATATTGTATCTGTC AACACTTTAGTTTTGGGACATAGTGAGCCTGGAGAAGCATCATTTTTACCTTGTAGCTCTCAATCATCAG AAGAATCCTCTAGACAGTCCTGTATGAGTGTACCTGGAATGTCTACTGGAACAGTACTTGGTGCTGGTAG TATTGGAACAGATATTGCAAATGAAATTCTGGATTTACAGAGGTTACAGTCTTCTTCATACCTTGATGAT TCGAGTCCAACAGGTTTAATGAAAGATACTCATACTGTAAACTGCAGGAGTATGTCAAATAAGGAGTTGT TTCCACCAAGTCCTTCTGAAATGGGGGAGCTAGAGGCTACCAGGCAAAACCAGAGTACTGTTGCTGTCCA CAGCCATGAGCCACTCCTCAGTGATGGTGCACAGTTGGATTTCGATGCCCTATGTGACAATGATGACACA GCCATGGCTGCATTTATGAATTACTTAGAAGCAGAGGGGGGCCTGGGAGACCCTGGGGACTTCAGTGACA TCCAGTGGACCCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234501 representing NM_001248004 Red=Cloning site Green=Tags(s) MAAEEEAAAGGKVLREENQCIAPVVSSRVSPGTRPTAMGSFSSHMTEFPRKRKGSDSDPSQEAHSQTEKR RRDKMNNLIEELSAMIPQCNPMARKLDKLTVLRMAVQHLRSLKGLTNSYVGSNYRPSFLQDNELRHLILK TAEGFLFVVGCERGKILFVSKSVSKILNYDQASLTGQSLFDFLHPKDVAKVKEQLSSFDISPREKLIDAK TGLQVHSNLHAGRTRVYSGSRRSFFCRIKSCKISVKEEHGCLPNSKKKEHRKFYTIHCTGYLRSWPPNIV GMEEERNSKKDNSNFTCLVAIGRLQPYIVPQNSGEINVKPTEFITRFAVNGKFVYVDQRATAILGYLPQE LLGTSCYEYFHQDDHNNLTDKHKAVLQSKEKILTDSYKFRAKDGSFVTLKSQWFSFTNPWTKELEYIVSV NTLVLGHSEPGEASFLPCSSQSSEESSRQSCMSVPGMSTGTVLGAGSIGTDIANEILDLQRLQSSSYLDD SSPTGLMKDTHTVNCRSMSNKELFPPSPSEMGELEATRQNQSTVAVHSHEPLLSDGAQLDFDALCDNDDT AMAAFMNYLEAEGGLGDPGDFSDIQWTL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001248004
ORF Size	1764 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001248004.2
RefSeq Size	7267 bp
RefSeq ORF	1767 bp
Locus ID	56938
UniProt ID	Q8WYA1
Protein Families	Druggable Genome, Transcription Factors
MW	66 kDa
Gene Summary	This gene encodes a basic helix-loop-helix transcription factor belonging to the PAS (PER, ARNT, SIM) superfamily. The PAS proteins play important roles in adaptation to low atmospheric and cellular oxygen levels, exposure to certain environmental pollutants, and diurnal oscillations in light and temperature. This protein forms a transcriptionally active heterodimer with the circadian CLOCK protein, the structurally related MOP4, and hypoxia-inducible factors, such as HIF1alpha. Consistent with its role as a biologically relevant partner of circadian and hypoxia factors, this protein is coexpressed in regions of the brain such as the thalamus, hypothalamus, and amygdala. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011]

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