United States
Germany
Japan
United Kingdom
China
GPR22 (NM_005295) Human Tagged ORF Clone
CAT＃: RG213524
- TrueORF®
GPR22 (tGFP-tagged) - Human G protein-coupled receptor 22 (GPR22)
ORF Plasmid: DDK
"NM_005295" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 4,750.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG213524 representing NM_005295
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGTTTTTCTCCCATTCTGGAAATCAACATGCAGTCTGAATCTAACATTACAGTGCGAGATGACATTG ATGACATCAACACCAATATGTACCAACCACTATCATATCCGTTAAGCTTTCAAGTGTCTCTCACCGGATT TCTTATGTTAGAAATTGTGTTGGGACTTGGCAGCAACCTCACTGTATTGGTACTTTACTGCATGAAATCC AACTTAATCAACTCTGTCAGTAACATTATTACAATGAATCTTCATGTACTTGATGTAATAATTTGTGTGG GATGTATTCCTCTAACTATAGTTATCCTTCTGCTTTCACTGGAGAGTAACACTGCTCTCATTTGCTGTTT CCATGAGGCTTGTGTATCTTTTGCAAGTGTCTCAACAGCAATCAACGTTTTTGCTATCACTTTGGACAGA TATGACATCTCTGTAAAACCTGCAAACCGAATTCTGACAATGGGCAGAGCTGTAGTGTTAATGATATCCA TTTGGATTTTTTCTTTTTTCTCTTTCCTGATTCCTTTTATTGAGGTAAATTTTTTCAGTCTTCAAAGTGG AAATACCTGGGAAAACAAGACACTTTTATGTGTCAGTACAAATGAATACTACACTGAACTGGGAATGTAT TATCACCTGTTAGTACAGATCCCAATATTCTTTTTCACTGTTGTAGTAATGTTAATCACATACACCAAAA TACTTCAGGCTCTTAATATTCGAATAGGCACAAGATTTTCAACAGGGCAGAAGAAGAAAGCAAGAAAGAA AAAGACAATTTCTCTAACCACACAACATGAGGCTACAGACATGTCACAAAGCAGTGGTGGGAGAAATGTA GTCTTTGGTGTAAGAACTTCAGTTTCTGTAATAATTGCCCTCCGGCGAGCTGTGAAACGACACCGTGAAC GACGAGAAAGACAAAAGAGAGTCTTCAGGATGTCTTTATTGATTATTTCTACATTTCTTCTCTGCTGGAC ACCAATTTCTGTTTTAAATACCACCATTTTATGTTTAGGCCCAAGTGACCTTTTAGTAAAATTAAGATTG TGTTTTTTAGTCATGGCTTATGGAACAACTATATTTCACCCTCTATTATATGCATTCACTAGACAAAAAT TTCAAAAGGTCTTGAAAAGTAAAATGAAAAAGCGAGTTGTTTCTATAGTAGAAGCTGATCCCCTGCCTAA TAATGCTGTAATACACAACTCTTGGATAGATCCTAAAAGAAACAAAAAAATTACCTTTGAAGATAGTGAA ATAAGAGAAAAATGTTTAGTGCCTCAGGTTGTCACAGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG213524 representing NM_005295
Red=Cloning site Green=Tags(s) MCFSPILEINMQSESNITVRDDIDDINTNMYQPLSYPLSFQVSLTGFLMLEIVLGLGSNLTVLVLYCMKS NLINSVSNIITMNLHVLDVIICVGCIPLTIVILLLSLESNTALICCFHEACVSFASVSTAINVFAITLDR YDISVKPANRILTMGRAVVLMISIWIFSFFSFLIPFIEVNFFSLQSGNTWENKTLLCVSTNEYYTELGMY YHLLVQIPIFFFTVVVMLITYTKILQALNIRIGTRFSTGQKKKARKKKTISLTTQHEATDMSQSSGGRNV VFGVRTSVSVIIALRRAVKRHRERRERQKRVFRMSLLIISTFLLCWTPISVLNTTILCLGPSDLLVKLRL CFLVMAYGTTIFHPLLYAFTRQKFQKVLKSKMKKRVVSIVEADPLPNNAVIHNSWIDPKRNKKITFEDSE IREKCLVPQVVTD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005295 |
| ORF Size | 1299 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005295.3 |
| RefSeq Size | 1302 bp |
| RefSeq ORF | 1302 bp |
| Locus ID | 2845 |
| UniProt ID | Q99680 |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| Gene Summary | This gene is a member of the G-protein coupled receptor 1 family and encodes a multi-pass membrane protein. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213524 | GPR22 (Myc-DDK-tagged)-Human G protein-coupled receptor 22 (GPR22) |
CNY 4,280.00 |
|
| RC213524L3 | Lenti-ORF clone of GPR22 (Myc-DDK-tagged)-Human G protein-coupled receptor 22 (GPR22) |
CNY 6,180.00 |
|
| RC213524L4 | Lenti-ORF clone of GPR22 (mGFP-tagged)-Human G protein-coupled receptor 22 (GPR22) |
CNY 6,056.00 |
|
| SC116801 | GPR22 (untagged)-Human G protein-coupled receptor 22 (GPR22) |
CNY 3,656.00 |
