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PKC nu (PRKD3) (NM_005813) Human Tagged ORF Clone
CAT＃: RG221309
- TrueORF®
PRKD3 (tGFP-tagged) - Human protein kinase D3 (PRKD3)
ORF Plasmid: DDK
"NM_005813" in other vectors (7)
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CNY 7,890.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | EPK2; nPKC-NU; PKC-NU; PKD3; PRKCN |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG221309 representing NM_005813
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGCAAATAATTCCCCTCCATCAGCCCAGAAGTCTGTATTACCCACAGCTATTCCTGCTGTGCTTC CAGCTGCTTCTCCGTGTTCAAGTCCTAAGACGGGACTCTCTGCCCGACTCTCTAATGGAAGCTTCAGTGC ACCATCACTCACCAACTCCAGAGGCTCAGTGCATACAGTTTCATTTCTACTGCAAATTGGCCTCACACGG GAGAGTGTTACCATTGAAGCCCAGGAACTGTCTTTATCTGCTGTCAAGGATCTTGTGTGCTCCATAGTTT ATCAAAAGTTTCCAGAGTGTGGATTCTTTGGCATGTATGACAAAATTCTTCTCTTTCGCCATGACATGAA CTCAGAAAACATTTTGCAGCTGATTACCTCAGCAGATGAAATACATGAAGGAGACCTAGTGGAAGTGGTT CTTTCAGCTTTAGCCACAGTAGAAGACTTCCAGATTCGTCCACATACTCTCTATGTACATTCTTACAAAG CTCCTACTTTCTGTGATTACTGTGGTGAGATGCTCTGGGGATTGGTACGTCAAGGACTGAAATGTGAAGG CTGTGGATTAAATTACCATAAACGATGTGCCTTCAAGATTCCAAATAACTGTAGTGGAGTAAGAAAGAGA CGTCTGTCAAATGTATCTTTACCAGGACCCGGCCTCTCAGTTCCAAGACCCCTACAGCCTGAATATGTAG CCCTTCCCAGTGAAGAGTCACATGTCCACCAGGAACCAAGTAAGAGAATTCCTTCTTGGAGTGGTCGCCC AATCTGGATGGAAAAGATGGTAATGTGCAGAGTGAAAGTTCCACACACATTTGCTGTTCACTCTTACACC CGTCCCACGATATGTCAGTACTGCAAGCGGTTACTGAAAGGCCTCTTTCGCCAAGGAATGCAGTGTAAAG ATTGCAAATTCAACTGCCATAAATGCTGTGCATCAAAAGTACCAAGAGACTGCCTTGGAGAGGTTACTTT CAATGGAGAACCTTCCAGTCTGGGAACAGATACAGATATACCAATGGATATTGACAATAATGACATAAAT AGTGATAGTAGTCGGGGTTTGGATGACACAGAAGAGCCATCACCCCCAGAAGATAAGATGTTCTTCTTGG ACCCATCTGATCTCGATGTGGAAAGAGATGAAGAAGCCGTTAAAACAATCAGTCCATCAACAAGCAATAA TATTCCGCTCATGAGGGTTGTACAATCCATCAAGCACACAAAGAGGAAGAGCAGCACAATGGTGAAGGAA GGGTGGATGGTCCATTACACCAGCAGGGATAACCTGAGAAAGAGGCATTATTGGAGACTTGACAGCAAAT GTCTAACATTATTTCAGAATGAATCTGGATCAAAGTATTATAAGGAAATTCCACTTTCAGAAATTCTCCG CATATCTTCACCACGAGATTTCACAAACATTTCACAAGGCAGCAATCCACACTGTTTTGAAATCATTACT GATACTATGGTATACTTCGTTGGTGAGAACAATGGGGACAGCTCTCATAATCCTGTTCTTGCTGCCACTG GAGTTGGACTTGATGTAGCACAGAGCTGGGAAAAAGCAATTCGCCAAGCCCTCATGCCTGTTACTCCTCA AGCAAGTGTTTGCACTTCTCCAGGGCAAGGGAAAGATCACAAAGATTTGTCTACAAGTATCTCTGTATCT AATTGTCAGATTCAGGAGAATGTGGATATCAGTACTGTTTACCAGATCTTTGCAGATGAGGTGCTTGGTT CAGGCCAGTTTGGCATCGTTTATGGAGGAAAACATAGAAAGACTGGGAGGGATGTGGCTATTAAAGTAAT TGATAAGATGAGATTCCCCACAAAACAAGAAAGTCAACTCCGTAATGAAGTGGCTATTTTACAGAATTTG CACCATCCTGGGATTGTAAACCTGGAATGTATGTTTGAAACCCCAGAACGAGTCTTTGTAGTAATGGAAA AGCTGCATGGAGATATGTTGGAAATGATTCTATCCAGTGAGAAAAGTCGGCTTCCAGAACGAATTACTAA ATTCATGGTCACACAGATACTTGTTGCTTTGAGGAATCTGCATTTTAAGAATATTGTGCACTGTGATTTA AAGCCAGAAAATGTGCTGCTTGCATCAGCAGAGCCATTTCCTCAGGTGAAGCTGTGTGACTTTGGATTTG CACGCATCATTGGTGAAAAGTCATTCAGGAGATCTGTGGTAGGAACTCCAGCATACTTAGCCCCTGAAGT TCTCCGGAGCAAAGGTTACAACCGTTCCCTAGATATGTGGTCAGTGGGAGTTATCATCTATGTGAGCCTC AGTGGCACATTTCCTTTTAATGAGGATGAAGATATAAATGACCAAATCCAAAATGCTGCATTTATGTACC CACCAAATCCATGGAGAGAAATTTCTGGTGAAGCAATTGATCTGATAAACAATCTGCTTCAAGTGAAGAT GAGAAAACGTTACAGTGTTGACAAATCTCTTAGTCATCCCTGGCTACAGGACTATCAGACTTGGCTTGAC CTTAGAGAATTTGAAACTCGCATTGGAGAACGTTACATTACACATGAAAGTGATGATGCTCGCTGGGAAA TACATGCATACACACATAACCTTGTATACCCAAAGCACTTCATTATGGCTCCTAATCCAGATGATATGGA AGAAGATCCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG221309 representing NM_005813
Red=Cloning site Green=Tags(s) MSANNSPPSAQKSVLPTAIPAVLPAASPCSSPKTGLSARLSNGSFSAPSLTNSRGSVHTVSFLLQIGLTR ESVTIEAQELSLSAVKDLVCSIVYQKFPECGFFGMYDKILLFRHDMNSENILQLITSADEIHEGDLVEVV LSALATVEDFQIRPHTLYVHSYKAPTFCDYCGEMLWGLVRQGLKCEGCGLNYHKRCAFKIPNNCSGVRKR RLSNVSLPGPGLSVPRPLQPEYVALPSEESHVHQEPSKRIPSWSGRPIWMEKMVMCRVKVPHTFAVHSYT RPTICQYCKRLLKGLFRQGMQCKDCKFNCHKCCASKVPRDCLGEVTFNGEPSSLGTDTDIPMDIDNNDIN SDSSRGLDDTEEPSPPEDKMFFLDPSDLDVERDEEAVKTISPSTSNNIPLMRVVQSIKHTKRKSSTMVKE GWMVHYTSRDNLRKRHYWRLDSKCLTLFQNESGSKYYKEIPLSEILRISSPRDFTNISQGSNPHCFEIIT DTMVYFVGENNGDSSHNPVLAATGVGLDVAQSWEKAIRQALMPVTPQASVCTSPGQGKDHKDLSTSISVS NCQIQENVDISTVYQIFADEVLGSGQFGIVYGGKHRKTGRDVAIKVIDKMRFPTKQESQLRNEVAILQNL HHPGIVNLECMFETPERVFVVMEKLHGDMLEMILSSEKSRLPERITKFMVTQILVALRNLHFKNIVHCDL KPENVLLASAEPFPQVKLCDFGFARIIGEKSFRRSVVGTPAYLAPEVLRSKGYNRSLDMWSVGVIIYVSL SGTFPFNEDEDINDQIQNAAFMYPPNPWREISGEAIDLINNLLQVKMRKRYSVDKSLSHPWLQDYQTWLD LREFETRIGERYITHESDDARWEIHAYTHNLVYPKHFIMAPNPDDMEEDP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005813 |
| ORF Size | 2670 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005813.3, NP_005804.1 |
| RefSeq Size | 5907 bp |
| RefSeq ORF | 2673 bp |
| Locus ID | 23683 |
| UniProt ID | O94806 |
| Domains | pkinase, TyrKc, PH, DAG_PE-bind, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | This gene belongs to the multigene protein kinase D family of serine/threonine kinases, which bind diacylglycerol and phorbol esters. Members of this family are characterized by an N-terminal regulatory domain comprised of a tandem repeat of cysteine-rich zinc-finger motifs and a pleckstrin domain. The C-terminal region contains the catalytic domain and is distantly related to calcium-regulated kinases. Catalytic activity of this enzyme promotes its nuclear localization. This protein has been implicated in a variety of functions including negative regulation of human airway epithelial barrier formation, growth regulation of breast and prostate cancer cells, and vesicle trafficking. [provided by RefSeq, Jan 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221309 | PRKD3 (Myc-DDK-tagged)-Human protein kinase D3 (PRKD3) |
CNY 7,130.00 |
|
| RC221309L1 | Lenti ORF clone of Human protein kinase D3 (PRKD3), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC221309L2 | Lenti ORF clone of Human protein kinase D3 (PRKD3), mGFP tagged |
CNY 9,030.00 |
|
| RC221309L3 | Lenti ORF clone of Human protein kinase D3 (PRKD3), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC221309L4 | Lenti ORF clone of Human protein kinase D3 (PRKD3), mGFP tagged |
CNY 9,030.00 |
|
| SC116506 | PRKD3 (untagged)-Human protein kinase D3 (PRKD3) |
CNY 6,528.00 |
|
| SC323561 | PRKD3 (untagged)-Kinase deficient mutant (K605M) of Human protein kinase D3 (PRKD3) |
CNY 6,528.00 |
