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NOX5 (NM_001184780) Human Tagged ORF Clone
CAT＃: RG230463
- TrueORF®
NOX5 (tGFP-tagged) - Human NADPH oxidase, EF-hand calcium binding domain 5 (NOX5), transcript variant 3
ORF Plasmid: DDK
"NM_001184780" in other vectors (6)
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CNY 6,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG230463 representing NM_001184780
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTTCGTCTGTGCCGGCTTATCCGACACCATGAGTGCCGAGGAGGATGCCAGGTGGCTCCGGTGGG TGACTCAGCAGTTTAAGACCATTGCAGGAGAAGATGGGGAGATCAGCCTGCAAGAATTCAAAGCAGCTCT GCATGTGAAAGAGTCCTTCTTTGCAGAGCGATTCTTTGCCCTATTTGACTCCGATAGAAGTGGCACCATC ACCCTCCAGGAGCTGCAGGAGGCACTGACCCTGCTCATCCATGGCAGCCCCATGGACAAACTCAAATTCC TCTTCCAGGTGTATGACATCGATGGCAGTGGCTCCATTGACCCGGATGAGCTGCGCACTGTGCTGCAGTC GTGTCTGCGCGAGAGCGCCATCTCGCTGCCTGACGAGAAGCTGGACCAGCTGACGCTGGCGCTCTTCGAA TCGGCCGACGCGGACGGCAACGGGGCCATCACCTTCGAGGAGCTCCGGGACGAGCTGCAGCGCTTCCCCG GAGTCATGGAGAACCTGACCATCAGCGCTGCCCACTGGCTGACGGCCCCCGCCCCCCGCCCACGCCCGCG CCGGCCGCGCCAGCTGACCCGCGCCTACTGGCACAACCACCGCAGCCAGCTGTTCTGCCTGGCCACCTAT GCAGGCCTCCACGTGCTGCTCTTCGGGCTGGCGGCCAGCGCGCACCGGGACCTCGGCGCCAGCGTCATGG TGGCCAAGGGCTGCGGCCAGTGCCTCAACTTCGACTGCAGCTTCATCGCGGTGCTGATGCTCAGACGCTG CCTCACCTGGCTGCGGGCCACGTGGCTGGCTCAAGTCCTACCACTGGACCAGAACATCCAGTTCCACCAG CTTATGGGCTACGTGGTAGTGGGGCTGTCCCTCGTGCACACCGTGGCTCACACTGTGAACTTTGTACTCC AGGCTCAGGCGGAGGCCAGCCCTTTCCAGTTCTGGGAGCTGCTGCTCACCACGAGGCCTGGCATTGGCTG GGTACACGGTTCGGCCTCCCCGACAGGTGTCGCTCTGCTGCTGCTGCTCCTCCTCATGTTCATCTGCTCC AGTTCCTGCATCCGCAGGAGTGGCCACTTTGAGGTGTTCTATTGGACTCACCTGTCCTACCTCCTCGTGT GGCTTCTGCTCATCTTTCATGGGCCCAACTTCTGGAAGTGGCTGCTGGTGCCTGGAATCTTGTTTTTCCT GGAGAAGGCCATCGGACTGGCAGTGTCCCGCATGGCAGCCGTGTGCATCATGGAAGTCAACCTCCTCCCC TCCAAGGTCACTCATCTCCTCATCAAGCGGCCCCCTTTTTTTCACTATAGACCTGGTGACTACTTGTATC TGAACATCCCCACCATTGCTCGCTATGAGTGGCACCCCTTCACCATCAGCAGTGCTCCTGAGCAGAAAGA CACTATCTGGCTGCACATTCGGTCCCAAGGCCAGTGGACAAACAGGCTGTATGAGTCCTTCAAGGCATCA GACCCACTGGGCCGTGGTTCTAAGAGGCTGTCGAGGAGTGTGACAATGAGAAAGAGTCAAAGGTCGTCCA AGGGCTCTGAGATACTTTTGGAGAAACACAAATTCTGTAACATCAAGTGCTACATCGATGGGCCTTATGG GACCCCCACCCGCAGGATCTTTGCCTCTGAGCATGCCGTGCTCATCGGGGCAGGCATCGGCATCACCCCC TTTGCTTCCATTCTGCAGAGTATCATGTACAGGCACCAGAAAAGAAAGCATACTTGCCCCAGCTGCCAGC ACTCCTGGATCGAAGGTGTCCAAGACAACATGAAGCTCCATAAGGTGGACTTTATCTGGATCAACAGAGA CCAGCGGTCTTTCGAGTGGTTTGTGAGCCTGCTGACTAAACTGGAGATGGACCAGGCCGAGGAGGCTCAA TACGGCCGCTTCCTGGAGCTGCATATGTACATGACATCTGCACTGGGCAAGAATGACATGAAGGCCATTG GCCTGCAGATGGCCCTTGACCTCCTGGCCAACAAGGAGAAGAAAGACTCCATCACGGGGCTGCAGACGCG CACCCAGCCTGGGCGGCCTGACTGGAGCAAGGTGTTCCAGAAAGTGGCTGCTGAGAAGAAGGGCAAGGTG CAGGTCTTCTTCTGTGGCTCCCCAGCTCTGGCCAAGGTGCTGAAGGGCCATTGTGAGAAGTTCGGCTTCA GATTTTTCCAAGAGAATTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG230463 representing NM_001184780
Red=Cloning site Green=Tags(s) MAFVCAGLSDTMSAEEDARWLRWVTQQFKTIAGEDGEISLQEFKAALHVKESFFAERFFALFDSDRSGTI TLQELQEALTLLIHGSPMDKLKFLFQVYDIDGSGSIDPDELRTVLQSCLRESAISLPDEKLDQLTLALFE SADADGNGAITFEELRDELQRFPGVMENLTISAAHWLTAPAPRPRPRRPRQLTRAYWHNHRSQLFCLATY AGLHVLLFGLAASAHRDLGASVMVAKGCGQCLNFDCSFIAVLMLRRCLTWLRATWLAQVLPLDQNIQFHQ LMGYVVVGLSLVHTVAHTVNFVLQAQAEASPFQFWELLLTTRPGIGWVHGSASPTGVALLLLLLLMFICS SSCIRRSGHFEVFYWTHLSYLLVWLLLIFHGPNFWKWLLVPGILFFLEKAIGLAVSRMAAVCIMEVNLLP SKVTHLLIKRPPFFHYRPGDYLYLNIPTIARYEWHPFTISSAPEQKDTIWLHIRSQGQWTNRLYESFKAS DPLGRGSKRLSRSVTMRKSQRSSKGSEILLEKHKFCNIKCYIDGPYGTPTRRIFASEHAVLIGAGIGITP FASILQSIMYRHQKRKHTCPSCQHSWIEGVQDNMKLHKVDFIWINRDQRSFEWFVSLLTKLEMDQAEEAQ YGRFLELHMYMTSALGKNDMKAIGLQMALDLLANKEKKDSITGLQTRTQPGRPDWSKVFQKVAAEKKGKV QVFFCGSPALAKVLKGHCEKFGFRFFQENF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001184780 |
| ORF Size | 2190 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001184780.2 |
| RefSeq Size | 2852 bp |
| RefSeq ORF | 2193 bp |
| Locus ID | 79400 |
| UniProt ID | Q96PH1 |
| Protein Families | Ion Channels: Other, Transmembrane |
| Gene Summary | This gene is predominantly expressed in the testis and lymphocyte-rich areas of spleen and lymph nodes. It encodes a calcium-dependen NADPH oxidase that generates superoxide, and functions as a calcium-dependent proton channel that may regulate redox-dependent processes in lymphocytes and spermatozoa. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230463 | NOX5 (Myc-DDK-tagged)-Human NADPH oxidase, EF-hand calcium binding domain 5 (NOX5), transcript variant 3 |
CNY 8,552.00 |
|
| RC230463L1 | Lenti-ORF clone of NOX5 (Myc-DDK-tagged)-Human NADPH oxidase, EF-hand calcium binding domain 5 (NOX5), transcript variant 3 |
CNY 7,700.00 |
|
| RC230463L2 | Lenti-ORF clone of NOX5 (mGFP-tagged)-Human NADPH oxidase, EF-hand calcium binding domain 5 (NOX5), transcript variant 3 |
CNY 7,700.00 |
|
| RC230463L3 | Lenti-ORF clone of NOX5 (Myc-DDK-tagged)-Human NADPH oxidase, EF-hand calcium binding domain 5 (NOX5), transcript variant 3 |
CNY 7,700.00 |
|
| RC230463L4 | Lenti-ORF clone of NOX5 (mGFP-tagged)-Human NADPH oxidase, EF-hand calcium binding domain 5 (NOX5), transcript variant 3 |
CNY 7,700.00 |
|
| SC329101 | NOX5 (untagged)-Human NADPH oxidase EF-hand calcium binding domain 5 (NOX5) transcript variant 3 |
CNY 7,220.00 |
