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Ankyrin G (ANK3) (NM_001204404) Human Tagged ORF Clone
CAT＃: RG235343
- TrueORF®
ANK3 (tGFP-tagged) - Homo sapiens ankyrin 3, node of Ranvier (ankyrin G) (ANK3), transcript variant 4
ORF Plasmid: DDK
"NM_001204404" in other vectors (2)
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CNY 20,520.00
货期*
14周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ANKYRIN-G; MRT37 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG235343 representing NM_001204404
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGAAGAACCAAAGGAGAAAAATGCAAAACCTGCTCATAGGAAAAGGAAAGGAAAAAAGTCTGATG CCAATGCAAGTTACTTAAGAGCAGCTCGAGCTGGACACCTTGAAAAGGCCCTCGACTACATAAAAAATGG AGTTGACATCAACATTTGCAATCAGAATGGGTTGAACGCTCTCCACCTTGCTTCCAAAGAAGGCCATGTA GAGGTTGTTTCTGAGCTGCTGCAGAGAGAAGCCAATGTGGATGCAGCTACAAAGAAAGGAAACACAGCAT TGCACATCGCATCTTTGGCTGGGCAAGCAGAGGTGGTAAAAGTCTTGGTTACAAATGGAGCCAATGTCAA TGCACAATCTCAGAATGGTTTCACGCCATTGTATATGGCAGCCCAGGAAAATCACCTGGAAGTTGTCAAG TTTCTTCTTGACAATGGTGCAAGCCAGAGCCTAGCCACAGAGGATGGCTTCACACCATTGGCAGTGGCTT TGCAACAAGGTCACGACCAAGTCGTTTCGCTCCTGCTAGAGAATGACACCAAAGGAAAAGTGCGTCTCCC AGCTCTTCATATCGCGGCCCGAAAAGACGACACGAAAGCCGCCGCCCTGCTGCTGCAGAATGACAACAAT GCAGATGTGGAATCAAAGAGTGGCTTCACTCCGCTCCACATAGCTGCTCACTATGGAAATATCAATGTAG CCACGTTGCTGTTAAACCGAGCGGCTGCTGTGGATTTCACCGCAAGGAATGACATCACTCCTTTACATGT TGCATCAAAAAGAGGAAATGCAAATATGGTAAAACTATTGCTCGATCGAGGAGCTAAAATCGATGCCAAA ACCAGGGATGGTCTGACACCACTGCACTGTGGAGCAAGGAGTGGCCACGAGCAGGTGGTAGAAATGTTGC TTGATCGAGCTGCCCCCATTCTTTCAAAAACCAAGAATGGATTATCTCCATTGCACATGGCCACACAAGG GGATCATTTAAACTGCGTCCAGCTTCTCCTCCAGCATAATGTACCCGTGGATGATGTCACCAATGACTAC CTGACTGCCCTACACGTGGCTGCCCACTGTGGCCATTACAAAGTTGCCAAGGTTCTCTTGGATAAGAAAG CTAACCCCAATGCCAAAGCCCTGAATGGCTTTACCCCTCTTCATATTGCCTGCAAGAAGAATCGAATTAA AGTAATGGAACTCCTTCTGAAACACGGTGCATCCATCCAAGCTGTAACCGAGTCGGGCCTTACCCCAATC CATGTTGCTGCCTTCATGGGGCATGTAAATATTGTATCACAACTAATGCATCATGGAGCCTCACCAAACA CCACCAATGTGAGAGGAGAAACAGCACTGCACATGGCAGCTCGCTCCGGCCAAGCTGAAGTTGTGCGGTA TCTGGTACAAGACGGAGCTCAGGTAGAAGCTAAAGCTAAGGATGACCAAACACCACTCCACATTTCAGCC CGACTGGGGAAAGCAGACATAGTACAACAGCTGTTGCAGCAAGGGGCATCTCCAAATGCAGCCACAACTT CTGGGTACACCCCACTTCACCTTTCCGCCCGAGAGGGGCATGAGGATGTGGCCGCGTTCCTTTTGGATCA TGGAGCGTCTTTATCTATAACAACAAAGAAAGGATTTACTCCTCTTCATGTGGCAGCAAAATATGGAAAG CTTGAAGTCGCCAATCTCCTGCTACAGAAAAGTGCATCTCCAGATGCTGCTGGGAAGAGCGGGCTAACAC CACTGCATGTAGCTGCACATTACGATAATCAGAAAGTGGCCCTTCTGCTTTTGGACCAAGGAGCCTCACC TCACGCAGCCGCAAAGAATGGTTATACGCCACTGCACATCGCTGCCAAAAAGAACCAGATGGACATAGCG ACAACTCTGCTGGAATATGGTGCTGATGCCAACGCAGTTACCCGGCAAGGAATTGCTTCCGTCCATCTCG CAGCTCAGGAAGGGCACGTGGACATGGTGTCGCTGCTCCTCGGTAGAAATGCGAATGTGAACCTGAGCAA TAAGAGCGGCCTGACCCCACTCCATTTGGCTGCTCAAGAAGATCGAGTGAATGTGGCAGAAGTCCTCGTA AACCAAGGGGCTCATGTGGACGCCCAGACAAAGATGGGATACACACCACTGCATGTGGGCTGCCACTATG GAAATATCAAGATTGTTAATTTCCTGCTCCAGCATTCTGCAAAAGTTAATGCCAAAACAAAGAATGGGTA TACGCCATTACATCAAGCAGCACAGCAGGGGCATACGCATATAATAAATGTCTTACTTCAGAACAACGCC TCCCCCAATGAACTCACTGTGAATGGGAATACTGCCCTTGGCATTGCCCGGCGCCTCGGCTACATCTCAG TAGTGGACACCCTGAAGATAGTGACCGAAGAGACCATGACCACAACTACTGTCACAGAGAAGCACAAAAT GAATGTTCCAGAAACGATGAATGAAGTTCTTGATATGTCTGATGATGAAGTTCGTAAAGCCAATGCCCCT GAAATGCTCAGTGATGGCGAATATATCTCAGATGTTGAAGAAGGTAATAGATGCACATGGTACAAAATTC CCAAGGTACAAGAGTTTACGGTGAAAAGTGAAGATGCAATGACCGGGGACACAGACAAATATCTTGGGCC ACAGGACCTTAAGGAATTGGGTGATGATTCCCTGCCTGCAGAGGGTTACATGGGCTTTAGTCTCGGAGCG CGTTCTGCCAGCCTCCGCTCCTTCAGTTCGGATAGGTCTTACACCTTGAACAGAAGCTCCTATGCACGGG ACAGCATGATGATTGAAGAACTCCTTGTGCCATCCAAAGAGCAGCATCTAACATTCACAAGGGAATTTGA TTCAGATTCTCTTAGACATTACAGCTGGGCTGCAGACACCTTAGACAATGTCAATCTTGTTTCAAGCCCC ATTCATTCTGGGTTTCTGGTTAGCTTTATGGTGGACGCGAGAGGGGGCTCCATGAGAGGAAGCCGTCATC ACGGGATGAGAATCATCATTCCTCCACGCAAGTGTACGGCCCCCACTCGAATCACCTGCCGTTTGGTAAA GAGACATAAACTGGCCAACCCACCCCCCATGGTGGAAGGAGAGGGATTAGCCAGTAGGCTGGTAGAAATG GGTCCTGCAGGGGCACAATTTTTAGGCCCTGTCATAGTGGAAATCCCTCACTTTGGGTCCATGAGAGGAA AAGAGAGAGAACTCATTGTTCTTCGAAGTGAAAATGGTGAAACTTGGAAGGAGCATCAGTTTGACAGCAA AAATGAAGATTTAACCGAGTTACTTAATGGCATGGATGAAGAACTTGATAGCCCAGAAGAGTTAGGGAAA AAGCGTATCTGCAGGATTATCACGAAAGATTTCCCCCAGTATTTTGCAGTGGTTTCCCGGATTAAGCAGG AAAGCAACCAGATTGGTCCTGAAGGTGGAATTCTGAGCAGCACCACAGTGCCCCTTGTTCAAGCATCTTT CCCAGAGGGTGCCCTAACTAAAAGAATTCGAGTGGGCCTCCAGGCCCAGCCTGTTCCAGATGAAATTGTG AAAAAGATCCTTGGAAACAAAGCAACTTTTAGCCCAATTGTCACTGTGGAACCAAGAAGACGGAAATTCC ATAAACCAATCACAATGACCATTCCGGTGCCCCCGCCCTCAGGAGAAGGTGTATCCAATGGATACAAAGG GGACACTACACCCAATCTGCGTCTTCTCTGTAGCATTACAGGGGGCACTTCGCCTGCTCAGTGGGAAGAC ATCACAGGAACAACTCCTTTGACGTTTATAAAAGATTGTGTCTCCTTTACAACCAATGTTTCAGCCAGAT TTTGGCTTGCAGACTGCCATCAAGTTTTAGAAACTGTGGGGTTAGCCACGCAACTGTACAGAGAATTGAT ATGTGTTCCATATATGGCCAAGTTTGTTGTTTTTGCCAAAATGAATGATCCCGTAGAATCTTCCTTGCGA TGTTTCTGCATGACAGATGACAAAGTGGACAAAACTTTAGAGCAACAAGAGAATTTTGAGGAAGTCGCAA GAAGCAAAGATATTGAGGTTCTGGAAGGAAAACCTATTTATGTTGATTGTTATGGAAATTTGGCCCCACT TACCAAAGGAGGACAGCAACTTGTTTTTAACTTTTATTCTTTCAAAGAAAATAGACTGCCATTTTCCATC AAGATTAGAGACACCAGCCAAGAGCCCTGTGGTCGTCTGTCTTTTCTGAAAGAACCAAAGACAACAAAAG GACTGCCTCAAACAGCGGTTTGCAACTTAAATATCACTCTGCCAGCACATAAAAAGATTGAGAAAACAGA TAGACGACAGAGCTTCGCATCCTTAGCTTTACGTAAGCGCTACAGCTACTTGACTGAGCCTGGAATGAGT CCACAGAGTCCATGTGAACGGACAGATATCAGGATGGCAATAGTAGCCGATCACCTGGGACTTAGTTGGA CAGAACTGGCAAGGGAACTGAATTTTTCAGTGGATGAAATCAATCAAATACGTGTGGAAAATCCAAATTC TTTAATTTCTCAGAGCTTCATGTTATTAAAAAAATGGGTTACCAGAGACGGAAAAAATGCCACAACTGAT GCCTTAACTTCGGTCTTGACAAAAATTAATCGAATAGATATAGTGACACTGCTAGAAGGACCAATATTTG ATTATGGAAATATTTCAGGCACCAGAAGTTTTGCAGATGAGAACAATGTTTTCCATGACCCTGTTGATGG TTATCCTTCCCTTCAAGTGGAACTGGAAACCCCCACAGGGTTGCACTACACACCACCTACCCCTTTCCAG CAAGATGATTATTTTAGTGATATCTCTAGCATAGAATCTCCCCTTAGAACCCCTAGTAGACTGAGTGATG GGCTAGTGCCTTCCCAGGGGAACATAGAGCATTCCGCAGATGGACCTCCAGTCGTAACTGCAGAAGACGC TTCCTTAGAAGACAGCAAACTGGAAGACTCAGTGCCTTTAACAGAAATGCCTGAAGCAGTGGATGTAGAT GAGAGCCAGTTGGAGAATGTATGTCTGAGTTGGCAGAATGAGACATCAAGTGGAAACCTAGAGTCCTGCG CTCAAGCTCGAAGAGTAACTGGTGGGTTACTAGATCGACTGGATGACAGCCCTGACCAGTGTAGAGATTC CATTACCTCATATCTCAAAGGAGAAGCTGGCAAATTTGAAGCAAATGGAAGCCATACAGAAATCACTCCA GAAGCAAAGACAAAATCTTACTTTCCAGAATCCCAAAATGATGTAGGAAAACAGAGTACCAAGGAAACTC TGAAACCAAAAATACATGGATCTGGTCATGTTGAAGAACCAGCATCACCACTAGCAGCATATCAGAAATC TCTAGAAGAAACCAGCAAGCTTATAATAGAAGAGACTAAACCCTGTGTGCCTGTCAGTATGAAAAAGATG AGTAGGACTTCTCCAGCAGATGGCAAGCCAAGGCTTAGCCTCCATGAAGAAGAGGGGTCCAGTGGGTCTG AGCAAAAGCAGGGAGAAGGTTTTAAGGTGAAAACGAAGAAAGAAATCCGGCATGTGGAAAAGAAGAGCCA CTCG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG235343 representing NM_001204404
Red=Cloning site Green=Tags(s) MSEEPKEKNAKPAHRKRKGKKSDANASYLRAARAGHLEKALDYIKNGVDINICNQNGLNALHLASKEGHV EVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVK FLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDNN ADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAK TRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDY LTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPI HVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISA RLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGK LEVANLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIA TTLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLGRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLV NQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHIINVLLQNNA SPNELTVNGNTALGIARRLGYISVVDTLKIVTEETMTTTTVTEKHKMNVPETMNEVLDMSDDEVRKANAP EMLSDGEYISDVEEGNRCTWYKIPKVQEFTVKSEDAMTGDTDKYLGPQDLKELGDDSLPAEGYMGFSLGA RSASLRSFSSDRSYTLNRSSYARDSMMIEELLVPSKEQHLTFTREFDSDSLRHYSWAADTLDNVNLVSSP IHSGFLVSFMVDARGGSMRGSRHHGMRIIIPPRKCTAPTRITCRLVKRHKLANPPPMVEGEGLASRLVEM GPAGAQFLGPVIVEIPHFGSMRGKERELIVLRSENGETWKEHQFDSKNEDLTELLNGMDEELDSPEELGK KRICRIITKDFPQYFAVVSRIKQESNQIGPEGGILSSTTVPLVQASFPEGALTKRIRVGLQAQPVPDEIV KKILGNKATFSPIVTVEPRRRKFHKPITMTIPVPPPSGEGVSNGYKGDTTPNLRLLCSITGGTSPAQWED ITGTTPLTFIKDCVSFTTNVSARFWLADCHQVLETVGLATQLYRELICVPYMAKFVVFAKMNDPVESSLR CFCMTDDKVDKTLEQQENFEEVARSKDIEVLEGKPIYVDCYGNLAPLTKGGQQLVFNFYSFKENRLPFSI KIRDTSQEPCGRLSFLKEPKTTKGLPQTAVCNLNITLPAHKKIEKTDRRQSFASLALRKRYSYLTEPGMS PQSPCERTDIRMAIVADHLGLSWTELARELNFSVDEINQIRVENPNSLISQSFMLLKKWVTRDGKNATTD ALTSVLTKINRIDIVTLLEGPIFDYGNISGTRSFADENNVFHDPVDGYPSLQVELETPTGLHYTPPTPFQ QDDYFSDISSIESPLRTPSRLSDGLVPSQGNIEHSADGPPVVTAEDASLEDSKLEDSVPLTEMPEAVDVD ESQLENVCLSWQNETSSGNLESCAQARRVTGGLLDRLDDSPDQCRDSITSYLKGEAGKFEANGSHTEITP EAKTKSYFPESQNDVGKQSTKETLKPKIHGSGHVEEPASPLAAYQKSLEETSKLIIEETKPCVPVSMKKM SRTSPADGKPRLSLHEEEGSSGSEQKQGEGFKVKTKKEIRHVEKKSHS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001204404 |
| ORF Size | 5604 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001204404.2 |
| RefSeq Size | 9599 bp |
| RefSeq ORF | 5607 bp |
| Locus ID | 288 |
| UniProt ID | Q12955 |
| Protein Families | Druggable Genome |
| Gene Summary | Ankyrins are a family of proteins that are believed to link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact, and the maintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various target proteins are expressed in a tissue-specific, developmentally regulated manner. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. Ankyrin 3 is an immunologically distinct gene product from ankyrins 1 and 2, and was originally found at the axonal initial segment and nodes of Ranvier of neurons in the central and peripheral nervous systems. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2011] |
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