Ankyrin G (ANK3) (NM_001204404) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ANKYRIN-G; MRT37
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331571 representing NM_001204404. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAGTGAAGAACCAAAGGAGAAAAATGCAAAACCTGCTCATAGGAAAAGGAAAGGAAAAAAGTCTGAT GCCAATGCAAGTTACTTAAGAGCAGCTCGAGCTGGACACCTTGAAAAGGCCCTCGACTACATAAAAAAT GGAGTTGACATCAACATTTGCAATCAGAATGGGTTGAACGCTCTCCACCTTGCTTCCAAAGAAGGCCAT GTAGAGGTTGTTTCTGAGCTGCTGCAGAGAGAAGCCAATGTGGATGCAGCTACAAAGAAAGGAAACACA GCATTGCACATCGCATCTTTGGCTGGGCAAGCAGAGGTGGTAAAAGTCTTGGTTACAAATGGAGCCAAT GTCAATGCACAATCTCAGAATGGTTTCACGCCATTGTATATGGCAGCCCAGGAAAATCACCTGGAAGTT GTCAAGTTTCTTCTTGACAATGGTGCAAGCCAGAGCCTAGCCACAGAGGATGGCTTCACACCATTGGCA GTGGCTTTGCAACAAGGTCACGACCAAGTCGTTTCGCTCCTGCTAGAGAATGACACCAAAGGAAAAGTG CGTCTCCCAGCTCTTCATATCGCGGCCCGAAAAGACGACACGAAAGCCGCCGCCCTGCTGCTGCAGAAT GACAACAATGCAGATGTGGAATCAAAGAGTGGCTTCACTCCGCTCCACATAGCTGCTCACTATGGAAAT ATCAATGTAGCCACGTTGCTGTTAAACCGAGCGGCTGCTGTGGATTTCACCGCAAGGAATGACATCACT CCTTTACATGTTGCATCAAAAAGAGGAAATGCAAATATGGTAAAACTATTGCTCGATCGAGGAGCTAAA ATCGATGCCAAAACCAGGGATGGTCTGACACCACTGCACTGTGGAGCAAGGAGTGGCCACGAGCAGGTG GTAGAAATGTTGCTTGATCGAGCTGCCCCCATTCTTTCAAAAACCAAGAATGGATTATCTCCATTGCAC ATGGCCACACAAGGGGATCATTTAAACTGCGTCCAGCTTCTCCTCCAGCATAATGTACCCGTGGATGAT GTCACCAATGACTACCTGACTGCCCTACACGTGGCTGCCCACTGTGGCCATTACAAAGTTGCCAAGGTT CTCTTGGATAAGAAAGCTAACCCCAATGCCAAAGCCCTGAATGGCTTTACCCCTCTTCATATTGCCTGC AAGAAGAATCGAATTAAAGTAATGGAACTCCTTCTGAAACACGGTGCATCCATCCAAGCTGTAACCGAG TCGGGCCTTACCCCAATCCATGTTGCTGCCTTCATGGGGCATGTAAATATTGTATCACAACTAATGCAT CATGGAGCCTCACCAAACACCACCAATGTGAGAGGAGAAACAGCACTGCACATGGCAGCTCGCTCCGGC CAAGCTGAAGTTGTGCGGTATCTGGTACAAGACGGAGCTCAGGTAGAAGCTAAAGCTAAGGATGACCAA ACACCACTCCACATTTCAGCCCGACTGGGGAAAGCAGACATAGTACAACAGCTGTTGCAGCAAGGGGCA TCTCCAAATGCAGCCACAACTTCTGGGTACACCCCACTTCACCTTTCCGCCCGAGAGGGGCATGAGGAT GTGGCCGCGTTCCTTTTGGATCATGGAGCGTCTTTATCTATAACAACAAAGAAAGGATTTACTCCTCTT CATGTGGCAGCAAAATATGGAAAGCTTGAAGTCGCCAATCTCCTGCTACAGAAAAGTGCATCTCCAGAT GCTGCTGGGAAGAGCGGGCTAACACCACTGCATGTAGCTGCACATTACGATAATCAGAAAGTGGCCCTT CTGCTTTTGGACCAAGGAGCCTCACCTCACGCAGCCGCAAAGAATGGTTATACGCCACTGCACATCGCT GCCAAAAAGAACCAGATGGACATAGCGACAACTCTGCTGGAATATGGTGCTGATGCCAACGCAGTTACC CGGCAAGGAATTGCTTCCGTCCATCTCGCAGCTCAGGAAGGGCACGTGGACATGGTGTCGCTGCTCCTC GGTAGAAATGCGAATGTGAACCTGAGCAATAAGAGCGGCCTGACCCCACTCCATTTGGCTGCTCAAGAA GATCGAGTGAATGTGGCAGAAGTCCTCGTAAACCAAGGGGCTCATGTGGACGCCCAGACAAAGATGGGA TACACACCACTGCATGTGGGCTGCCACTATGGAAATATCAAGATTGTTAATTTCCTGCTCCAGCATTCT GCAAAAGTTAATGCCAAAACAAAGAATGGGTATACGCCATTACATCAAGCAGCACAGCAGGGGCATACG CATATAATAAATGTCTTACTTCAGAACAACGCCTCCCCCAATGAACTCACTGTGAATGGGAATACTGCC CTTGGCATTGCCCGGCGCCTCGGCTACATCTCAGTAGTGGACACCCTGAAGATAGTGACCGAAGAGACC ATGACCACAACTACTGTCACAGAGAAGCACAAAATGAATGTTCCAGAAACGATGAATGAAGTTCTTGAT ATGTCTGATGATGAAGTTCGTAAAGCCAATGCCCCTGAAATGCTCAGTGATGGCGAATATATCTCAGAT GTTGAAGAAGGTAATAGATGCACATGGTACAAAATTCCCAAGGTACAAGAGTTTACGGTGAAAAGTGAA GATGCAATGACCGGGGACACAGACAAATATCTTGGGCCACAGGACCTTAAGGAATTGGGTGATGATTCC CTGCCTGCAGAGGGTTACATGGGCTTTAGTCTCGGAGCGCGTTCTGCCAGCCTCCGCTCCTTCAGTTCG GATAGGTCTTACACCTTGAACAGAAGCTCCTATGCACGGGACAGCATGATGATTGAAGAACTCCTTGTG CCATCCAAAGAGCAGCATCTAACATTCACAAGGGAATTTGATTCAGATTCTCTTAGACATTACAGCTGG GCTGCAGACACCTTAGACAATGTCAATCTTGTTTCAAGCCCCATTCATTCTGGGTTTCTGGTTAGCTTT ATGGTGGACGCGAGAGGGGGCTCCATGAGAGGAAGCCGTCATCACGGGATGAGAATCATCATTCCTCCA CGCAAGTGTACGGCCCCCACTCGAATCACCTGCCGTTTGGTAAAGAGACATAAACTGGCCAACCCACCC CCCATGGTGGAAGGAGAGGGATTAGCCAGTAGGCTGGTAGAAATGGGTCCTGCAGGGGCACAATTTTTA GGCCCTGTCATAGTGGAAATCCCTCACTTTGGGTCCATGAGAGGAAAAGAGAGAGAACTCATTGTTCTT CGAAGTGAAAATGGTGAAACTTGGAAGGAGCATCAGTTTGACAGCAAAAATGAAGATTTAACCGAGTTA CTTAATGGCATGGATGAAGAACTTGATAGCCCAGAAGAGTTAGGGAAAAAGCGTATCTGCAGGATTATC ACGAAAGATTTCCCCCAGTATTTTGCAGTGGTTTCCCGGATTAAGCAGGAAAGCAACCAGATTGGTCCT GAAGGTGGAATTCTGAGCAGCACCACAGTGCCCCTTGTTCAAGCATCTTTCCCAGAGGGTGCCCTAACT AAAAGAATTCGAGTGGGCCTCCAGGCCCAGCCTGTTCCAGATGAAATTGTGAAAAAGATCCTTGGAAAC AAAGCAACTTTTAGCCCAATTGTCACTGTGGAACCAAGAAGACGGAAATTCCATAAACCAATCACAATG ACCATTCCGGTGCCCCCGCCCTCAGGAGAAGGTGTATCCAATGGATACAAAGGGGACACTACACCCAAT CTGCGTCTTCTCTGTAGCATTACAGGGGGCACTTCGCCTGCTCAGTGGGAAGACATCACAGGAACAACT CCTTTGACGTTTATAAAAGATTGTGTCTCCTTTACAACCAATGTTTCAGCCAGATTTTGGCTTGCAGAC TGCCATCAAGTTTTAGAAACTGTGGGGTTAGCCACGCAACTGTACAGAGAATTGATATGTGTTCCATAT ATGGCCAAGTTTGTTGTTTTTGCCAAAATGAATGATCCCGTAGAATCTTCCTTGCGATGTTTCTGCATG ACAGATGACAAAGTGGACAAAACTTTAGAGCAACAAGAGAATTTTGAGGAAGTCGCAAGAAGCAAAGAT ATTGAGGTTCTGGAAGGAAAACCTATTTATGTTGATTGTTATGGAAATTTGGCCCCACTTACCAAAGGA GGACAGCAACTTGTTTTTAACTTTTATTCTTTCAAAGAAAATAGACTGCCATTTTCCATCAAGATTAGA GACACCAGCCAAGAGCCCTGTGGTCGTCTGTCTTTTCTGAAAGAACCAAAGACAACAAAAGGACTGCCT CAAACAGCGGTTTGCAACTTAAATATCACTCTGCCAGCACATAAAAAGATTGAGAAAACAGATAGACGA CAGAGCTTCGCATCCTTAGCTTTACGTAAGCGCTACAGCTACTTGACTGAGCCTGGAATGAGTCCACAG AGTCCATGTGAACGGACAGATATCAGGATGGCAATAGTAGCCGATCACCTGGGACTTAGTTGGACAGAA CTGGCAAGGGAACTGAATTTTTCAGTGGATGAAATCAATCAAATACGTGTGGAAAATCCAAATTCTTTA ATTTCTCAGAGCTTCATGTTATTAAAAAAATGGGTTACCAGAGACGGAAAAAATGCCACAACTGATGCC TTAACTTCGGTCTTGACAAAAATTAATCGAATAGATATAGTGACACTGCTAGAAGGACCAATATTTGAT TATGGAAATATTTCAGGCACCAGAAGTTTTGCAGATGAGAACAATGTTTTCCATGACCCTGTTGATGGT TATCCTTCCCTTCAAGTGGAACTGGAAACCCCCACAGGGTTGCACTACACACCACCTACCCCTTTCCAG CAAGATGATTATTTTAGTGATATCTCTAGCATAGAATCTCCCCTTAGAACCCCTAGTAGACTGAGTGAT GGGCTAGTGCCTTCCCAGGGGAACATAGAGCATTCCGCAGATGGACCTCCAGTCGTAACTGCAGAAGAC GCTTCCTTAGAAGACAGCAAACTGGAAGACTCAGTGCCTTTAACAGAAATGCCTGAAGCAGTGGATGTA GATGAGAGCCAGTTGGAGAATGTATGTCTGAGTTGGCAGAATGAGACATCAAGTGGAAACCTAGAGTCC TGCGCTCAAGCTCGAAGAGTAACTGGTGGGTTACTAGATCGACTGGATGACAGCCCTGACCAGTGTAGA GATTCCATTACCTCATATCTCAAAGGAGAAGCTGGCAAATTTGAAGCAAATGGAAGCCATACAGAAATC ACTCCAGAAGCAAAGACAAAATCTTACTTTCCAGAATCCCAAAATGATGTAGGAAAACAGAGTACCAAG GAAACTCTGAAACCAAAAATACATGGATCTGGTCATGTTGAAGAACCAGCATCACCACTAGCAGCATAT CAGAAATCTCTAGAAGAAACCAGCAAGCTTATAATAGAAGAGACTAAACCCTGTGTGCCTGTCAGTATG AAAAAGATGAGTAGGACTTCTCCAGCAGATGGCAAGCCAAGGCTTAGCCTCCATGAAGAAGAGGGGTCC AGTGGGTCTGAGCAAAAGCAGGGAGAAGGTTTTAAGGTGAAAACGAAGAAAGAAATCCGGCATGTGGAA AAGAAGAGCCACTCGTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001204404
Insert Size	5607 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001204404.1
RefSeq Size	9599 bp
RefSeq ORF	5607 bp
Locus ID	288
UniProt ID	Q12955
Protein Families	Druggable Genome
MW	204.1 kDa
Gene Summary	Ankyrins are a family of proteins that are believed to link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact, and the maintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various target proteins are expressed in a tissue-specific, developmentally regulated manner. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. Ankyrin 3 is an immunologically distinct gene product from ankyrins 1 and 2, and was originally found at the axonal initial segment and nodes of Ranvier of neurons in the central and peripheral nervous systems. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2011] Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region, initiates translation at an alternate start codon, and lacks an alternate in-frame exon compared to variant 1. The encoded protein (isoform 4) has a distinct N-terminus and is considerably shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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