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Gria2 (NM_017261) Rat Tagged ORF Clone
CAT＃: RR200374
- TrueORF®
Gria2 (Myc-DDK-tagged ORF) - Rat glutamate receptor, ionotropic, AMPA 2 (Gria2), transcript variant 1, (10 ug)
"NM_017261" in other vectors (3)
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CNY 9,696.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GluA2; gluR-B; GluR-K2; GluR2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR200374 representing NM_017261
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAAAAGATTATGCATATTTCTGTCCTCCTTTCTCCTGTTTTATGGGGACTGATTTTTGGTGTCTCTT CTAACAGCATACAGATAGGGGGGCTATTTCCAAGGGGCGCTGATCAAGAATACAGTGCATTTCGGGTAGG GATGGTTCAGTTTTCCACTTCGGAGTTCAGACTGACACCCCATATCGACAATTTGGAGGTAGCCAACAGT TTCGCAGTCACCAATGCTTTCTGCTCCCAGTTTTCAAGAGGAGTCTACGCAATTTTTGGATTTTATGACA AGAAGTCTGTAAATACCATCACATCATTCTGTGGGACACTCCATGTGTCCTTCATCACACCTAGCTTCCC AACAGATGGCACACATCCATTTGTCATCCAGATGCGACCTGACCTCAAAGGAGCACTCCTTAGCTTGATT GAGTACTACCAATGGGACAAGTTCGCATACCTCTATGACAGTGACAGAGGCTTATCAACACTGCAAGCTG TTCTGGATTCTGCTGCAGAGAAGAAGTGGCAGGTGACTGCTATCAATGTGGGGAACATCAACAATGACAA GAAAGATGAGACCTACAGATCGCTCTTTCAAGATCTGGAGTTAAAAAAAGAACGGCGTGTAATCCTGGAC TGTGAAAGGGATAAAGTAAATGACATTGTGGACCAGGTTATTACCATTGGAAAACATGTTAAAGGGTACC ATTATATCATTGCAAATCTGGGATTCACTGATGGGGACCTGCTGAAAATTCAGTTTGGAGGAGCAAATGT CTCTGGATTTCAGATTGTAGACTACGACGATTCCCTGGTGTCTAAATTTATAGAAAGATGGTCAACACTG GAAGAGAAAGAATACCCTGGAGCACACACAGCGACAATTAAGTATACTTCGGCCCTGACGTATGATGCTG TCCAAGTGATGACTGAAGCATTCCGTAACCTTCGGAAGCAGAGGATTGAAATATCCCGGAGAGGAAATGC AGGAGATTGTTTGGCCAACCCAGCTGTGCCCTGGGGACAAGGGGTCGAAATAGAAAGGGCCCTCAAGCAG GTTCAAGTTGAAGGCCTCTCTGGAAATATAAAGTTTGACCAGAATGGAAAACGAATAAACTACACAATTA ACATCATGGAGCTCAAAACAAATGGACCCCGGAAGATTGGGTACTGGAGTGAAGTGGATAAAATGGTTGT CACCCTAACTGAGCTCCCATCAGGAAATGACACGTCTGGGCTTGAAAACAAGACTGTGGTGGTCACCACA ATATTGGAATCTCCATATGTTATGATGAAGAAAAATCATGAAATGCTTGAAGGGAATGAGCGTTACGAGG GCTACTGTGTTGACTTAGCTGCAGAAATTGCTAAACACTGTGGGTTCAAGTACAAGCTGACTATTGTTGG GGATGGCAAGTATGGGGCCAGGGATGCCGACACCAAAATTTGGAATGGTATGGTTGGAGAGCTTGTCTAC GGGAAAGCTGACATTGCAATTGCTCCATTAACTATCACTCTCGTGAGAGAAGAGGTGATTGACTTCTCCA AGCCCTTCATGAGTCTTGGAATCTCTATCATGATCAAGAAGCCTCAGAAGTCCAAACCAGGAGTGTTTTC CTTTCTTGATCCTTTAGCCTATGAGATCTGGATGTGCATTGTGTTTGCCTACATTGGGGTCAGTGTAGTT TTATTCCTGGTCAGCAGATTTAGCCCCTACGAGTGGCACACTGAGGAATTTGAAGATGGAAGAGAAACAC AAAGTAGTGAATCAACTAATGAATTTGGGATTTTTAATAGTCTCTGGTTTTCCTTGGGTGCCTTTATGCG GCAAGGATGCGATATTTCGCCAAGATCCCTCTCTGGGCGCATTGTTGGAGGTGTGTGGTGGTTCTTTACC CTGATCATAATCTCCTCCTACACGGCTAACTTAGCTGCCTTCCTGACTGTAGAGAGGATGGTGTCTCCCA TCGAAAGTGCTGAGGATCTGTCTAAGCAAACAGAAATTGCTTATGGAACATTAGACTCTGGCTCCACTAA AGAGTTTTTCAGGAGATCTAAAATCGCAGTGTTTGATAAAATGTGGACTTATATGAGGAGTGCAGAGCCC TCTGTGTTTGTGAGGACTACCGCAGAAGGAGTAGCCAGAGTCCGGAAATCCAAAGGAAAGTATGCCTACT TGCTGGAGTCCACAATGAACGAGTACATCGAGCAGAGGAAGCCTTGTGACACCATGAAAGTGGGAGGAAA CTTGGATTCCAAAGGCTACGGCATCGCCACACCTAAAGGATCCTCATTAGGAACCCCAGTAAATCTTGCA GTATTGAAACTCAGTGAGCAAGGCGTCTTAGACAAGCTGAAAAACAAATGGTGGTACGATAAAGGTGAAT GTGGAGCCAAGGACTCGGGAAGTAAGGAAAAGACCAGTGCCCTCAGTCTGAGCAACGTTGCTGGAGTATT CTACATCCTTGTCGGGGGCCTTGGTTTGGCAATGCTGGTGGCTTTGATTGAGTTCTGTTACAAGTCAAGG GCCGAGGCGAAACGAATGAAGGTGGCAAAGAATCCACAGAATATTAACCCATCTTCCTCGCAGAATTCCC AGAATTTTGCAACTTATAAGGAAGGTTACAACGTATATGGCATCGAGAGTGTTAAAATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR200374 representing NM_017261
Red=Cloning site Green=Tags(s) MQKIMHISVLLSPVLWGLIFGVSSNSIQIGGLFPRGADQEYSAFRVGMVQFSTSEFRLTPHIDNLEVANS FAVTNAFCSQFSRGVYAIFGFYDKKSVNTITSFCGTLHVSFITPSFPTDGTHPFVIQMRPDLKGALLSLI EYYQWDKFAYLYDSDRGLSTLQAVLDSAAEKKWQVTAINVGNINNDKKDETYRSLFQDLELKKERRVILD CERDKVNDIVDQVITIGKHVKGYHYIIANLGFTDGDLLKIQFGGANVSGFQIVDYDDSLVSKFIERWSTL EEKEYPGAHTATIKYTSALTYDAVQVMTEAFRNLRKQRIEISRRGNAGDCLANPAVPWGQGVEIERALKQ VQVEGLSGNIKFDQNGKRINYTINIMELKTNGPRKIGYWSEVDKMVVTLTELPSGNDTSGLENKTVVVTT ILESPYVMMKKNHEMLEGNERYEGYCVDLAAEIAKHCGFKYKLTIVGDGKYGARDADTKIWNGMVGELVY GKADIAIAPLTITLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVV LFLVSRFSPYEWHTEEFEDGRETQSSESTNEFGIFNSLWFSLGAFMRQGCDISPRSLSGRIVGGVWWFFT LIIISSYTANLAAFLTVERMVSPIESAEDLSKQTEIAYGTLDSGSTKEFFRRSKIAVFDKMWTYMRSAEP SVFVRTTAEGVARVRKSKGKYAYLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGIATPKGSSLGTPVNLA VLKLSEQGVLDKLKNKWWYDKGECGAKDSGSKEKTSALSLSNVAGVFYILVGGLGLAMLVALIEFCYKSR AEAKRMKVAKNPQNINPSSSQNSQNFATYKEGYNVYGIESVKI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017261 |
| ORF Size | 2649 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017261.2, NP_058957.1 |
| RefSeq Size | 6745 bp |
| RefSeq ORF | 2652 bp |
| Locus ID | 29627 |
| UniProt ID | P19491 |
| MW | 99.2 kDa |
| Gene Summary | Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. These channels are assembled from 4 related subunits, Gria1-4. The subunit encoded by this gene (Gria2) is subject to RNA editing (Q/R and R/G), which is thought to render the channels impermeable to Ca(2+), and to affect the kinetic aspects of these channels in rat brain. Alternative splicing, resulting in transcript variants encoding different isoforms (flip and flop), has been noted for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN200374 | Gria2 (untagged ORF) - Rat glutamate receptor, ionotropic, AMPA 2 (Gria2), transcript variant 1, (10 ug) |
CNY 11,310.00 |
|
| RR200374L3 | Lenti ORF clone of Gria2 (Myc-DDK-tagged ORF) - Rat glutamate receptor, ionotropic, AMPA 2 (Gria2), transcript variant 1, (10 ug) |
CNY 13,110.00 |
|
| RR200374L4 | Lenti ORF clone of Gria2 (mGFP-tagged ORF) - Rat glutamate receptor, ionotropic, AMPA 2 (Gria2), transcript variant 1, (10 ug) |
CNY 14,250.00 |
