Wnk1 (NM_053794) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Hsn2; Prkwnk1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR200644 representing NM_053794 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGACGGCACCGCAGAGAAGCAGAGCGGCACTCCTGGCTTCCTTTCGCCTCCGGCTCCAGTCCCCA AGAATGGCTCAAGCTCCGATTCTTCCGTGGGCGAGAAACTGGGGGCCGCGGTCGCCGACTCCGGAATTGG CAGGACAGAGGAATACCGGCGCCGCCGGCACACTATGGACAAGGACAGTCGTGGGGCGGCCGCCACCACT ACCCCCACGGAGCACCGATTTTTCCGCCGAAGCGTCATCTGCGACTCCAATGCCACGGCGTTGGAGCTGC CGGGCCTCCCTCTCTCTATCCCTCAACCTAGTGTACCCGCAGTGGTGCCTCAGAGTGCTCCACCCGAGCC TCACAGAGAGGAAACTTTGACGGCCACCGTTGCTTCCCAGGTGTCTCAGCAACCCTCGGCTGCTGCCTCC CCTGGGGAACAAGCCGTTGTAGGCTCGGCCACCGCGACCGTCCCCAGCAGTACCAGCAAAGACCGCCCGG TGTCCCAGCCCAGCCTCGTGGGTAGCAAAGAGGAGCCGCCGCCGTCCAGAAGTGGAAGTGGCAGCGGTGG TGCCAGTGCCAAAGAGCCGCAGGAGGAAAGGAACCAGCAACAGGATGATATCGAAGAGCTGGAGACCAAG GCAGTGGGAATGTCCAATGACGGTCGCTTTCTCAAATTTGACATCGAAATCGGCAGAGGCTCCTTTAAGA CGGTCTACAAAGGTCTGGACACCGAAACCACTGTGGAAGTCGCCTGGTGTGAATTGCAGGATCGAAAGTT AACAAAATCTGAAAGGCAGAGATTCAAAGAAGAAGCTGAAATGCTAAAGGGTCTTCAACATCCTAACATT GTTCGATTCTATGATTCCTGGGAATCCACAGTAAAAGGGAAAAAATGCATTGTTTTAGTGACTGAACTAA TGACATCTGGAACACTTAAAACGTACTTAAAAAGGTTTAAAGTGATGAAAATCAAAGTTTTAAGAAGCTG GTGTCGGCAAATCCTTAAAGGACTTCAATTTCTCCATACACGAACTCCCCCGATTATTCACCGGGATCTT AAATGTGACAACATCTTTATCACTGGTCCTACCGGCTCAGTCAAGATCGGAGACCTTGGTCTGGCAACTC TAAAGCGGGCTTCTTTTGCCAAGAGTGTGATAGGTACCCCAGAGTTTATGGCTCCTGAGATGTATGAGGA GAAGTACGATGAATCCGTTGATGTTTATGCTTTTGGGATGTGCATGCTTGAGATGGCTACGTCTGAATAT CCATACTCAGAGTGCCAAAATGCTGCTCAGATCTACCGTCGAGTGACCAGTGGAGTGAAGCCAGCCAGTT TTGACAAAGTAGCAATTCCTGAAGTGAAGGAAATTATTGAAGGATGTATCCGACAAAACAAAGATGAAAG ATATTCCATCAAAGACCTTTTGAACCATGCCTTTTTCCAGGAGGAAACAGGGGTACGGGTAGAATTAGCA GAAGAAGATGATGGAGAAAAGATAGCTATCAAATTATGGCTACGTATTGAAGATATTAAGAAATTAAAGG GAAAATACAAAGACAATGAAGCTATTGAGTTTTCCTTTGACTTGGAGAGGGATGTACCAGAAGATGTTGC TCAAGAAATGGTCGAGTCTGGTTATGTCTGTGAAGGTGATCACAAGACCATGGCTAAAGCTATCAAAGAT AGGGTATCATTAATTAAGAGAAAACGAGAGCAGCGGCAGTTGGTTCGAGAAGAACAAGAAAAAAGAAAAC AAGAAGAGAGCAGTTTCAAACAGCAGAATGAACAACAAGCAAGTGTTTCCCAGGCAGGAATCCAGCCACT CTCTGTTGCTAGCACTGGCATACCCACTGCTCCCACCACATCCGCTTCAGTTTCTACTCAAGTAGAGCCT GAAGAACCCGAGGCAGATCAGCATCAACAGCTGCAGTATCAGCAACCTAGTATATCTGTGTTGTCTGATG GAACAGTTGACAGTGGTCAAGGATCATCTGTATTCACAGAATCCAGAGTGAGCAGCCAACAGACTGTTTC CTATGGCTCCCAACATGAGCAGGCACATTCTATTGGCACAGCACCAGGACATACAGTTTCTAGTATCCAA GCACAATCTCAGCCCCATGGGGTGTATCCACCCTCAAGTATGGCACAGGGGCAGAACCAGGGCCAGCCAT CAAGTAGTTTAGCAGGGGTTCTATCTTCACAACCCGTCCAACATCCTCAGCAGCAGGGAATACAGCCGAC TGTACCTCCTCAACAGGCAGTACAGTATTCACTTCCACAGGCAGCGTCTTCCAGTGAAGGCACTGTCCAG CCAGTGAGTCAGCCTCAAGTGTCAGCTGGAACACAGAGTAGTACACAGGGAGTCTCTCAGGCTGCTCCTC CAGAACAAACTCCAATAACACAGTCACAACCTACTCAACCTGTTCCATTGGTTTCCTCTGTGGACAGTGC CCATTCAGATGTTGCTTCTGGTATGAGTGATGGCAATGAGAATGCTCCTTCATCCAGCGGGAGGCATGAA GGGAGGACGACAAAACGTCATTATCGAAAATCTGTAAGAAGTCGTTCTCGTCATGAAAAGACTTCTCGCC CGAAACTGAGGATTTTAAATGTTTCAAATAAAGGAGACCGAGTAGTAGAATGTCAATTGGAGACTCATAA TCGGAAAATGGTTACATTTAAATTTGACCTAGATGGTGACAACCCTGAAGAGATAGCAACAATTATGGTG AACAATGACTTTATTCTAGCCATAGAGAGAGAGTCTTTTGTGGCTCAAGTGCGGGAAATTATTGAAAAGG CTGATGAAATGTTAAGCGAGGATGTCAGTGTGGAGCCTGAGGGTGACCAGGGATTGGAGAGTCTACAAGG AAAGGATGACTATGGCTTTCCAGGTTCTCAGAAATTAGAAGGAGAGTTCAAGCAACCAATTGCTGTATCT TCCATGCCACAACAAATAGGTGTTCCTACCAGTTCTTTAACACAAGTTGTTCATTCTGCTGGAAGACGAT TTATAGTGAGTCCAGTTCCAGAAAGTCGATTACGAGAATCAAAAATTTTCACCAGTGAAATACCAGATCC AGTTGCGGCCTCTACATCTCAGGGCCCTGGAATGAACTTATCTCACTCCGCTTCATCCCTTAGTCTCCAG CAGGCTTTTTCTGAACTGAAACATGGTCAGATGACTGAAGGACCCAATACAGCACCTCCAAATTTTAATC ACCCAGGACCAACATTTTCTCCCTTTTTGACTAGCATTGCTGGTGTCCAAACTGTAGCAGCATCCACACC ATCAGTATCAGTCCCTATAACAAGCAGCCCTCTTAATGACATTTCCACATCAGTGATGCAGTCAGAGGGT GCACTGCCCACTGACAAAGGCATTGGGGGAGTTACCACCAGCACAGGTGTGGTAGCTTCAGGTGGTCTCA CCACATTGTCTGTGTCTGAGACACCAACACTTTCCAGTGCAGTTTCAAGTTCTACAGCGCCTGCAGTTGT CACAGTGTCTACAACATCCCAACCAGTTCAGGCTTCCACATCTGGGAGCATTGCTTCTAGTACAGGCAGT TTTCCTTCTGGAACATTTTCCACAACTACAGGTACCACAGTGAGTAGTGTAGCTGTCCCAAATGCTAAGC CTCCAACTGTATTACTGCAACAGGTAGCAGGTAATACTGCTGGGGTAGCCATAGTGACATCAGTTTCTAC TACCACTCCATTCCCAGCCATGGCTTCACAGCCATCCCTTCCACTTGGTAGCAGCACATCTGCTCCTACA CTAGCTGAAACAGTGGTGGTTAGTGCACACTCACTAGACAAGGCATCTCACAGTAGTACAGCAGGATTGG GTTTGTCCTTTTGCGCACCGTCCTCTTCTTCCTCCTCTGGAACAGCAGTGTCTAGTTCTGTTTCTCAGCC TGGTATTGTGCATCCTTTGGTCATCTCATCAGCTATAGCTTCTACACCTGTCCTCCCACAACCAGCAGTA CCTACTTCTACACCTTTATTACCCCAAGTACCTAATATCCCACCCTTGGTACAACCTGTTGCTAATGTGC CTGCTGTACAGCAAACACTCATTCATAGTCAGCCTCAACCAGCTCTACTTCCCAACCAGCCTCACACACA CTGTCCCGAAATGGATGCTGATACCCAGTCCAAAGCTCCTGGGATCGATGATATAAAGACTCTGGAGGAA AAGCTGAGATCGCTCTTTAGTGAGCATAGCTCATCTGGAACTCAGCATGCTTCTGTCTCCCTGGAGACAC CCTTAGTAGTAGAGACTGTCACACCGGGCATCCCTACCACTGCTGTAGCACCAAGTAAACTCATGACTTC CACTACAAGCACTTGCTTGCCACCAACCAATTTACCCTTAGGAACAGCTGGCATGCCAGTTATGCCAGTG GGCACACCTGGTCAAGTTTCTACCCCAGGCACACATGCCTCAGCCCCAGCCAGCACCGCAACAGGAGCAA AACCTGGAACAACTCCCCCAAAGCCATCTTTAACTAAGACTGTGGTGCCGCCAGTGGGTACTGAGCTTTC GGCTGGTACTGTACCCTGTGAGCAGCTGCCACCTTTTCCCGGACCGTCTCTAATTCAGACCCAGCAACCC CTAGAAGATCTTGATGCTCAGTTGAGAAGAACACTTAGTCCAGAGACTATTCCAGTGACACCCGCAGTTG GTCCTTTGTCTACGATGTCTTCAACGGCAGTTACAGAAGCAGGAAGTCAACCTCAGAAGGATGGCACAGA AGTTCATGTCACAGCAAGTAGCTCAGGAGCTGGTGTTGTTAAGATGGGGAGATTTCAGGTTTCAGTTACA ATGGATGATGCCCAGAAAGAGCGTAAAAATAGGTCAGAAGATACAAAGTCGGTTCATTTTGAATCTAGTA CATCAGAGTCATCAGTTCTTTCAAGTAGTAGTCCAGAGAGTACCCTGGTAAAGCCAGAGCCTAATGGAAT TACTGTTTCTGGCATCTCCTTAGATGTGCCAGACAGTACCCATAGAACACCGACCCCAGAAGCAAAGTCA GAAACAGGACAGCCTACCAAGGTTGGACGTTTTCAGGTGACAACTACAGCCAACAAAGTGGGTCGTTTCT CTGTTTCAAGAACTGAGGACAAGGTCACAGAGCTCAAAAAAGAGGGACCAGTGACCTCTCCTTTCAGAGA TTCAGAACAAACTGTTATTCCTGCTGCGATACCAAAGAAGGAGAAGCCTGAGCTGGCAGAACCTTCCCAT CTGAATGGGCCATCTTCAGACTTGGAGGCTGCCTTTCTAAGTAGGGGTGGAGAGGATGGATCAGGTAGTC CTCACTCTCCCCCTCACCTGTGTTCAAAGAGCCTTCCCATCCAGACTCTAAGTCAAAGCCTTAGTAATTC ATTCAACTCCTCTTACATGAGTAGTGACAATGAGTCCGACATTGAAGATGAAGACTTAAGGTTAGAACTG CGACGACTACGGGAAAAACATCTTAAAGAGATTCAGGACCTGCAGAGTCGCCAGAAGCATGAAATTGAAT CTTTGTATACCAAACTGGGCAAGGTTCCCCCTGCTGTCATTATTCCCCCAGCTGCTCCTCTGTCAGGGAG AAGAAGGAGGCCTACTAAAAGCAAAGGCAGCAAGTCCAGTCGCAGCAGCTCATTGGGCAACAAAAGCCCT CAGCTTTCAGGTAACCTGTCTGGTCAGAGTGGAACTTCAGTCTTAAACCCCCAACAGACCCTCCATCCCC CAGGCAATACCCCAGAGACTGGACACAACCAGCTGTTACAGCCTCTTAAGCCATCTCCCTCCAGTGACAA CCTCTATTCAGCCTTTACCAGTGATGGTGCCATTTCAGTACCAAGCCTTTCTGCTCCAGGTCAAGGGACC AGCAGCACAAACACCGTTGGAGGAACAGTGAGTAGCCAGGCTGCCCAGGCTCAGCCTCCTGCCATGACTT CCAGTAGGAAGGGCACATTCACAGATGACCTGCACAAGTTGGTAGACAACTGGGCCCGAGATGCCATGAA TCTTTCAGGCAGGAGAGGCAGCAAAGGACACATGAATTATGAGGGCCCTGGAATGGCAAGGAAGTTCTCT GCTCCTGGGCAGCTGTGCATTTCCATGACCTCAAACATGGGTGGCTCTACCCCCATCTCTGCAGCATCTG CTACCTCTCTAGGTCACTTCACCAAGTCCATGTGCCCCCCACAGCAGTATGGTTTTCCAGCTGCCCCATT CGGCACTCAGTGGAGTGGAACAGGTGGTCCAGCACCACAGCCACTTGGTCAGTTCCAACCTGTAGGAACC ACATCCTTGCAAAATTTCAACATCAGCAATTTGCAGAAGTCCATCAGCAACCCCCCAGGTTCCAACCTAC GGACCACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR200644 representing NM_053794 Red=Cloning site Green=Tags(s) MSDGTAEKQSGTPGFLSPPAPVPKNGSSSDSSVGEKLGAAVADSGIGRTEEYRRRRHTMDKDSRGAAATT TPTEHRFFRRSVICDSNATALELPGLPLSIPQPSVPAVVPQSAPPEPHREETLTATVASQVSQQPSAAAS PGEQAVVGSATATVPSSTSKDRPVSQPSLVGSKEEPPPSRSGSGSGGASAKEPQEERNQQQDDIEELETK AVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNI VRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDL KCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEY PYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELA EEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKD RVSLIKRKREQRQLVREEQEKRKQEESSFKQQNEQQASVSQAGIQPLSVASTGIPTAPTTSASVSTQVEP EEPEADQHQQLQYQQPSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSIGTAPGHTVSSIQ AQSQPHGVYPPSSMAQGQNQGQPSSSLAGVLSSQPVQHPQQQGIQPTVPPQQAVQYSLPQAASSSEGTVQ PVSQPQVSAGTQSSTQGVSQAAPPEQTPITQSQPTQPVPLVSSVDSAHSDVASGMSDGNENAPSSSGRHE GRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMV NNDFILAIERESFVAQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFPGSQKLEGEFKQPIAVS SMPQQIGVPTSSLTQVVHSAGRRFIVSPVPESRLRESKIFTSEIPDPVAASTSQGPGMNLSHSASSLSLQ QAFSELKHGQMTEGPNTAPPNFNHPGPTFSPFLTSIAGVQTVAASTPSVSVPITSSPLNDISTSVMQSEG ALPTDKGIGGVTTSTGVVASGGLTTLSVSETPTLSSAVSSSTAPAVVTVSTTSQPVQASTSGSIASSTGS FPSGTFSTTTGTTVSSVAVPNAKPPTVLLQQVAGNTAGVAIVTSVSTTTPFPAMASQPSLPLGSSTSAPT LAETVVVSAHSLDKASHSSTAGLGLSFCAPSSSSSSGTAVSSSVSQPGIVHPLVISSAIASTPVLPQPAV PTSTPLLPQVPNIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEMDADTQSKAPGIDDIKTLEE KLRSLFSEHSSSGTQHASVSLETPLVVETVTPGIPTTAVAPSKLMTSTTSTCLPPTNLPLGTAGMPVMPV GTPGQVSTPGTHASAPASTATGAKPGTTPPKPSLTKTVVPPVGTELSAGTVPCEQLPPFPGPSLIQTQQP LEDLDAQLRRTLSPETIPVTPAVGPLSTMSSTAVTEAGSQPQKDGTEVHVTASSSGAGVVKMGRFQVSVT MDDAQKERKNRSEDTKSVHFESSTSESSVLSSSSPESTLVKPEPNGITVSGISLDVPDSTHRTPTPEAKS ETGQPTKVGRFQVTTTANKVGRFSVSRTEDKVTELKKEGPVTSPFRDSEQTVIPAAIPKKEKPELAEPSH LNGPSSDLEAAFLSRGGEDGSGSPHSPPHLCSKSLPIQTLSQSLSNSFNSSYMSSDNESDIEDEDLRLEL RRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSP QLSGNLSGQSGTSVLNPQQTLHPPGNTPETGHNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGT SSTNTVGGTVSSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFS APGQLCISMTSNMGGSTPISAASATSLGHFTKSMCPPQQYGFPAAPFGTQWSGTGGPAPQPLGQFQPVGT TSLQNFNISNLQKSISNPPGSNLRTT TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_053794
ORF Size	6378 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_053794.2, NP_446246.2
RefSeq Size	8973 bp
RefSeq ORF	6381 bp
Locus ID	116477
UniProt ID	Q9JIH7
MW	225.1 kDa
Gene Summary	catalyzes the phosphorylation of myelin basic protein in vitro; has autophosphorylation activity; may regulate the thiazide-sensitive Na-Cl cotransporter NCC [RGD, Feb 2006]

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