Wnk1 (NM_053794) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Hsn2; Prkwnk1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN200644 representing NM_053794 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGACGGCACCGCAGAGAAGCAGAGCGGCACTCCTGGCTTCCTTTCGCCTCCGGCTCCAGTCCCCA AGAATGGCTCAAGCTCCGATTCTTCCGTGGGCGAGAAACTGGGGGCCGCGGTCGCCGACTCCGGAATTGG CAGGACAGAGGAATACCGGCGCCGCCGGCACACTATGGACAAGGACAGTCGTGGGGCGGCCGCCACCACT ACCCCCACGGAGCACCGATTTTTCCGCCGAAGCGTCATCTGCGACTCCAATGCCACGGCGTTGGAGCTGC CGGGCCTCCCTCTCTCTATCCCTCAACCTAGTGTACCCGCAGTGGTGCCTCAGAGTGCTCCACCCGAGCC TCACAGAGAGGAAACTTTGACGGCCACCGTTGCTTCCCAGGTGTCTCAGCAACCCTCGGCTGCTGCCTCC CCTGGGGAACAAGCCGTTGTAGGCTCGGCCACCGCGACCGTCCCCAGCAGTACCAGCAAAGACCGCCCGG TGTCCCAGCCCAGCCTCGTGGGTAGCAAAGAGGAGCCGCCGCCGTCCAGAAGTGGAAGTGGCAGCGGTGG TGCCAGTGCCAAAGAGCCGCAGGAGGAAAGGAACCAGCAACAGGATGATATCGAAGAGCTGGAGACCAAG GCAGTGGGAATGTCCAATGACGGTCGCTTTCTCAAATTTGACATCGAAATCGGCAGAGGCTCCTTTAAGA CGGTCTACAAAGGTCTGGACACCGAAACCACTGTGGAAGTCGCCTGGTGTGAATTGCAGGATCGAAAGTT AACAAAATCTGAAAGGCAGAGATTCAAAGAAGAAGCTGAAATGCTAAAGGGTCTTCAACATCCTAACATT GTTCGATTCTATGATTCCTGGGAATCCACAGTAAAAGGGAAAAAATGCATTGTTTTAGTGACTGAACTAA TGACATCTGGAACACTTAAAACGTACTTAAAAAGGTTTAAAGTGATGAAAATCAAAGTTTTAAGAAGCTG GTGTCGGCAAATCCTTAAAGGACTTCAATTTCTCCATACACGAACTCCCCCGATTATTCACCGGGATCTT AAATGTGACAACATCTTTATCACTGGTCCTACCGGCTCAGTCAAGATCGGAGACCTTGGTCTGGCAACTC TAAAGCGGGCTTCTTTTGCCAAGAGTGTGATAGGTACCCCAGAGTTTATGGCTCCTGAGATGTATGAGGA GAAGTACGATGAATCCGTTGATGTTTATGCTTTTGGGATGTGCATGCTTGAGATGGCTACGTCTGAATAT CCATACTCAGAGTGCCAAAATGCTGCTCAGATCTACCGTCGAGTGACCAGTGGAGTGAAGCCAGCCAGTT TTGACAAAGTAGCAATTCCTGAAGTGAAGGAAATTATTGAAGGATGTATCCGACAAAACAAAGATGAAAG ATATTCCATCAAAGACCTTTTGAACCATGCCTTTTTCCAGGAGGAAACAGGGGTACGGGTAGAATTAGCA GAAGAAGATGATGGAGAAAAGATAGCTATCAAATTATGGCTACGTATTGAAGATATTAAGAAATTAAAGG GAAAATACAAAGACAATGAAGCTATTGAGTTTTCCTTTGACTTGGAGAGGGATGTACCAGAAGATGTTGC TCAAGAAATGGTCGAGTCTGGTTATGTCTGTGAAGGTGATCACAAGACCATGGCTAAAGCTATCAAAGAT AGGGTATCATTAATTAAGAGAAAACGAGAGCAGCGGCAGTTGGTTCGAGAAGAACAAGAAAAAAGAAAAC AAGAAGAGAGCAGTTTCAAACAGCAGAATGAACAACAAGCAAGTGTTTCCCAGGCAGGAATCCAGCCACT CTCTGTTGCTAGCACTGGCATACCCACTGCTCCCACCACATCCGCTTCAGTTTCTACTCAAGTAGAGCCT GAAGAACCCGAGGCAGATCAGCATCAACAGCTGCAGTATCAGCAACCTAGTATATCTGTGTTGTCTGATG GAACAGTTGACAGTGGTCAAGGATCATCTGTATTCACAGAATCCAGAGTGAGCAGCCAACAGACTGTTTC CTATGGCTCCCAACATGAGCAGGCACATTCTATTGGCACAGCACCAGGACATACAGTTTCTAGTATCCAA GCACAATCTCAGCCCCATGGGGTGTATCCACCCTCAAGTATGGCACAGGGGCAGAACCAGGGCCAGCCAT CAAGTAGTTTAGCAGGGGTTCTATCTTCACAACCCGTCCAACATCCTCAGCAGCAGGGAATACAGCCGAC TGTACCTCCTCAACAGGCAGTACAGTATTCACTTCCACAGGCAGCGTCTTCCAGTGAAGGCACTGTCCAG CCAGTGAGTCAGCCTCAAGTGTCAGCTGGAACACAGAGTAGTACACAGGGAGTCTCTCAGGCTGCTCCTC CAGAACAAACTCCAATAACACAGTCACAACCTACTCAACCTGTTCCATTGGTTTCCTCTGTGGACAGTGC CCATTCAGATGTTGCTTCTGGTATGAGTGATGGCAATGAGAATGCTCCTTCATCCAGCGGGAGGCATGAA GGGAGGACGACAAAACGTCATTATCGAAAATCTGTAAGAAGTCGTTCTCGTCATGAAAAGACTTCTCGCC CGAAACTGAGGATTTTAAATGTTTCAAATAAAGGAGACCGAGTAGTAGAATGTCAATTGGAGACTCATAA TCGGAAAATGGTTACATTTAAATTTGACCTAGATGGTGACAACCCTGAAGAGATAGCAACAATTATGGTG AACAATGACTTTATTCTAGCCATAGAGAGAGAGTCTTTTGTGGCTCAAGTGCGGGAAATTATTGAAAAGG CTGATGAAATGTTAAGCGAGGATGTCAGTGTGGAGCCTGAGGGTGACCAGGGATTGGAGAGTCTACAAGG AAAGGATGACTATGGCTTTCCAGGTTCTCAGAAATTAGAAGGAGAGTTCAAGCAACCAATTGCTGTATCT TCCATGCCACAACAAATAGGTGTTCCTACCAGTTCTTTAACACAAGTTGTTCATTCTGCTGGAAGACGAT TTATAGTGAGTCCAGTTCCAGAAAGTCGATTACGAGAATCAAAAATTTTCACCAGTGAAATACCAGATCC AGTTGCGGCCTCTACATCTCAGGGCCCTGGAATGAACTTATCTCACTCCGCTTCATCCCTTAGTCTCCAG CAGGCTTTTTCTGAACTGAAACATGGTCAGATGACTGAAGGACCCAATACAGCACCTCCAAATTTTAATC ACCCAGGACCAACATTTTCTCCCTTTTTGACTAGCATTGCTGGTGTCCAAACTGTAGCAGCATCCACACC ATCAGTATCAGTCCCTATAACAAGCAGCCCTCTTAATGACATTTCCACATCAGTGATGCAGTCAGAGGGT GCACTGCCCACTGACAAAGGCATTGGGGGAGTTACCACCAGCACAGGTGTGGTAGCTTCAGGTGGTCTCA CCACATTGTCTGTGTCTGAGACACCAACACTTTCCAGTGCAGTTTCAAGTTCTACAGCGCCTGCAGTTGT CACAGTGTCTACAACATCCCAACCAGTTCAGGCTTCCACATCTGGGAGCATTGCTTCTAGTACAGGCAGT TTTCCTTCTGGAACATTTTCCACAACTACAGGTACCACAGTGAGTAGTGTAGCTGTCCCAAATGCTAAGC CTCCAACTGTATTACTGCAACAGGTAGCAGGTAATACTGCTGGGGTAGCCATAGTGACATCAGTTTCTAC TACCACTCCATTCCCAGCCATGGCTTCACAGCCATCCCTTCCACTTGGTAGCAGCACATCTGCTCCTACA CTAGCTGAAACAGTGGTGGTTAGTGCACACTCACTAGACAAGGCATCTCACAGTAGTACAGCAGGATTGG GTTTGTCCTTTTGCGCACCGTCCTCTTCTTCCTCCTCTGGAACAGCAGTGTCTAGTTCTGTTTCTCAGCC TGGTATTGTGCATCCTTTGGTCATCTCATCAGCTATAGCTTCTACACCTGTCCTCCCACAACCAGCAGTA CCTACTTCTACACCTTTATTACCCCAAGTACCTAATATCCCACCCTTGGTACAACCTGTTGCTAATGTGC CTGCTGTACAGCAAACACTCATTCATAGTCAGCCTCAACCAGCTCTACTTCCCAACCAGCCTCACACACA CTGTCCCGAAATGGATGCTGATACCCAGTCCAAAGCTCCTGGGATCGATGATATAAAGACTCTGGAGGAA AAGCTGAGATCGCTCTTTAGTGAGCATAGCTCATCTGGAACTCAGCATGCTTCTGTCTCCCTGGAGACAC CCTTAGTAGTAGAGACTGTCACACCGGGCATCCCTACCACTGCTGTAGCACCAAGTAAACTCATGACTTC CACTACAAGCACTTGCTTGCCACCAACCAATTTACCCTTAGGAACAGCTGGCATGCCAGTTATGCCAGTG GGCACACCTGGTCAAGTTTCTACCCCAGGCACACATGCCTCAGCCCCAGCCAGCACCGCAACAGGAGCAA AACCTGGAACAACTCCCCCAAAGCCATCTTTAACTAAGACTGTGGTGCCGCCAGTGGGTACTGAGCTTTC GGCTGGTACTGTACCCTGTGAGCAGCTGCCACCTTTTCCCGGACCGTCTCTAATTCAGACCCAGCAACCC CTAGAAGATCTTGATGCTCAGTTGAGAAGAACACTTAGTCCAGAGACTATTCCAGTGACACCCGCAGTTG GTCCTTTGTCTACGATGTCTTCAACGGCAGTTACAGAAGCAGGAAGTCAACCTCAGAAGGATGGCACAGA AGTTCATGTCACAGCAAGTAGCTCAGGAGCTGGTGTTGTTAAGATGGGGAGATTTCAGGTTTCAGTTACA ATGGATGATGCCCAGAAAGAGCGTAAAAATAGGTCAGAAGATACAAAGTCGGTTCATTTTGAATCTAGTA CATCAGAGTCATCAGTTCTTTCAAGTAGTAGTCCAGAGAGTACCCTGGTAAAGCCAGAGCCTAATGGAAT TACTGTTTCTGGCATCTCCTTAGATGTGCCAGACAGTACCCATAGAACACCGACCCCAGAAGCAAAGTCA GAAACAGGACAGCCTACCAAGGTTGGACGTTTTCAGGTGACAACTACAGCCAACAAAGTGGGTCGTTTCT CTGTTTCAAGAACTGAGGACAAGGTCACAGAGCTCAAAAAAGAGGGACCAGTGACCTCTCCTTTCAGAGA TTCAGAACAAACTGTTATTCCTGCTGCGATACCAAAGAAGGAGAAGCCTGAGCTGGCAGAACCTTCCCAT CTGAATGGGCCATCTTCAGACTTGGAGGCTGCCTTTCTAAGTAGGGGTGGAGAGGATGGATCAGGTAGTC CTCACTCTCCCCCTCACCTGTGTTCAAAGAGCCTTCCCATCCAGACTCTAAGTCAAAGCCTTAGTAATTC ATTCAACTCCTCTTACATGAGTAGTGACAATGAGTCCGACATTGAAGATGAAGACTTAAGGTTAGAACTG CGACGACTACGGGAAAAACATCTTAAAGAGATTCAGGACCTGCAGAGTCGCCAGAAGCATGAAATTGAAT CTTTGTATACCAAACTGGGCAAGGTTCCCCCTGCTGTCATTATTCCCCCAGCTGCTCCTCTGTCAGGGAG AAGAAGGAGGCCTACTAAAAGCAAAGGCAGCAAGTCCAGTCGCAGCAGCTCATTGGGCAACAAAAGCCCT CAGCTTTCAGGTAACCTGTCTGGTCAGAGTGGAACTTCAGTCTTAAACCCCCAACAGACCCTCCATCCCC CAGGCAATACCCCAGAGACTGGACACAACCAGCTGTTACAGCCTCTTAAGCCATCTCCCTCCAGTGACAA CCTCTATTCAGCCTTTACCAGTGATGGTGCCATTTCAGTACCAAGCCTTTCTGCTCCAGGTCAAGGGACC AGCAGCACAAACACCGTTGGAGGAACAGTGAGTAGCCAGGCTGCCCAGGCTCAGCCTCCTGCCATGACTT CCAGTAGGAAGGGCACATTCACAGATGACCTGCACAAGTTGGTAGACAACTGGGCCCGAGATGCCATGAA TCTTTCAGGCAGGAGAGGCAGCAAAGGACACATGAATTATGAGGGCCCTGGAATGGCAAGGAAGTTCTCT GCTCCTGGGCAGCTGTGCATTTCCATGACCTCAAACATGGGTGGCTCTACCCCCATCTCTGCAGCATCTG CTACCTCTCTAGGTCACTTCACCAAGTCCATGTGCCCCCCACAGCAGTATGGTTTTCCAGCTGCCCCATT CGGCACTCAGTGGAGTGGAACAGGTGGTCCAGCACCACAGCCACTTGGTCAGTTCCAACCTGTAGGAACC ACATCCTTGCAAAATTTCAACATCAGCAATTTGCAGAAGTCCATCAGCAACCCCCCAGGTTCCAACCTAC GGACCACCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_053794
Insert Size	6381 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_053794.2, NP_446246.2
RefSeq Size	8973 bp
RefSeq ORF	6381 bp
Locus ID	116477
UniProt ID	Q9JIH7
Gene Summary	catalyzes the phosphorylation of myelin basic protein in vitro; has autophosphorylation activity; may regulate the thiazide-sensitive Na-Cl cotransporter NCC [RGD, Feb 2006] Transcript Variant: This variant (3) has multiple differences in the coding region but maintains the reading frame compared to variant 1. The resulting protein (isoform 3) is shorter but has the same N- and C-termini compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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