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Metrnl (NM_001014104) Rat Tagged ORF Clone
CAT＃: RR204359
- TrueORF®
Metrnl (Myc-DDK-tagged ORF) - Rat meteorin, glial cell differentiation regulator-like (Metrnl), (10 ug)
"NM_001014104" in other vectors (3)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR204359 representing NM_001014104
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGGGTGTGGTGTGGGCGGCCCGGAGGCGCGCGGGGCAGCAGTGGCCTCGGTCCCCGGGCCCTGGGC CGGGTCCGCCCCCGCCGCCACCGCTGCTGTTGCTGCTACTGCTGCTGCTGGGCGGCGCGAGCGCGCAGTA CTCCAGCGACCTGTGCAGCTGGAAGGGGAGTGGGCTCACCCGGGAGGCACACAGCAAGGAGGTGGAGCAG GTGTACCTGCGCTGCTCAGCAGGCTCTGTGGAATGGATGTACCCAACCGGGGCGCTCATTGTTAACCTAC GGCCCAACACCTTCTCACCTGCCCAGAACTTGACTGTGTGCATCAAGCCTTTCAGGGACTCCTCTGGGGC CAATATTTATTTGGAAAAAACTGGAGAACTAAGACTGTTGGTGCGGGATGTCAGAGGCGAACCTGGCCAA GTGCAGTGCTTCAGCCTAGAGCAGGGAGGCTTATTTGTGGAGGCCACACCCCAGCAGGACATCAGCAGAA GGACCACAGGCTTCCAGTATGAGCTGATGAGTGGGCAGAGGGGACTGGACCTGCACGTGCTCTCTGCCCC CTGTCGACCTTGCAGCGACACTGAGGTCCTCCTTGCCATCTGCACCAGTGACTTTGTTGTCCGAGGCTTC ATCGAGGATGTCACCCATGTACCAGAACAGCAAGTGTCAGTCATTCACCTACGGGTGAGCAGGCTCCACA GGCAGAAGAGCAGGGTCTTCCAGCCAGCTCCTGAGGACAGTGGCCACTGGCTGGGCCATGTCACAACACT GTTGCAGTGTGGAGTACGACCAGGGCATGGAGAATTCCTCTTCACTGGACATGTGCACTTTGGGGAGGCA CAACTTGGATGTGCCCCACGCTTTAGTGACTTTCAAAAGATGTACAGGAAAGCAGAAGAAAGGGGCATAA ACCCTTGTGAAATAAATATGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR204359 representing NM_001014104
Red=Cloning site Green=Tags(s) MRGVVWAARRRAGQQWPRSPGPGPGPPPPPPLLLLLLLLLGGASAQYSSDLCSWKGSGLTREAHSKEVEQ VYLRCSAGSVEWMYPTGALIVNLRPNTFSPAQNLTVCIKPFRDSSGANIYLEKTGELRLLVRDVRGEPGQ VQCFSLEQGGLFVEATPQQDISRRTTGFQYELMSGQRGLDLHVLSAPCRPCSDTEVLLAICTSDFVVRGF IEDVTHVPEQQVSVIHLRVSRLHRQKSRVFQPAPEDSGHWLGHVTTLLQCGVRPGHGEFLFTGHVHFGEA QLGCAPRFSDFQKMYRKAEERGINPCEINME TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001014104 |
| ORF Size | 933 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001014104.1, NP_001014126.1 |
| RefSeq Size | 1380 bp |
| RefSeq ORF | 936 bp |
| Locus ID | 316842 |
| UniProt ID | Q5RJL6 |
| MW | 34.5 kDa |
| Gene Summary | Hormone induced following exercise or cold exposure that promotes energy expenditure. Induced either in the skeletal muscle after exercise or in adipose tissue following cold exposure and is present in the circulation. Able to stimulate energy expenditure associated with the browning of the white fat depots and improves glucose tolerance. Does not promote an increase in a thermogenic gene program via direct action on adipocytes, but acts by stimulating several immune cell subtypes to enter the adipose tissue and activate their prothermogenic actions. Stimulates an eosinophil-dependent increase in IL4 expression and promotes alternative activation of adipose tissue macrophages, which are required for the increased expression of the thermogenic and anti-inflammatory gene programs in fat. Required for some cold-induced thermogenic responses, suggesting a role in metabolic adaptations to cold temperatures (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN204359 | Metrnl (untagged ORF) - Rat meteorin, glial cell differentiation regulator-like (Metrnl), (10 ug) |
CNY 3,990.00 |
|
| RR204359L3 | Lenti ORF clone of Metrnl (Myc-DDK-tagged ORF) - Rat meteorin, glial cell differentiation regulator-like (Metrnl), (10 ug) |
CNY 6,080.00 |
|
| RR204359L4 | Lenti ORF clone of Metrnl (mGFP-tagged ORF) - Rat meteorin, glial cell differentiation regulator-like (Metrnl), (10 ug) |
CNY 6,650.00 |
