Cltc (NM_019299) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR207450 representing NM_019299 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCAGATTCTGCCAATTCGCTTTCAGGAGCATCTCCAGCTCCAGAACCTGGGTATCAACCCAGCAA ACATTGGCTTCAGTACCCTGACTATGGAGTCAGACAAATTCATCTGCATTAGAGAAAAAGTGGGAGAGCA GGCTCAGGTGGTGATCATTGACATGAATGATCCAAGTAATCCGATTCGAAGACCAATCTCAGCAGACAGT GCCATCATGAATCCTGCCAGCAAAGTGATTGCACTGAAAGCTGGAAAAACTCTACAGATATTTAACATTG AAATGAAAAGTAAAATGAAGGCCCACACCATGACCGATGATGTCACCTTCTGGAAATGGATCTCTCTGAA TACGGTTGCTCTTGTTACGGATAATGCAGTTTATCACTGGAGCATGGAGGGGGAGTCTCAGCCAGTGAAG ATGTTTGATCGTCATTCCAGCCTCGCAGGGTGCCAGATCATCAATTACCGTACAGATGCTAAGCAGAAGT GGTTGCTCCTGACTGGCATATCTGCACAGCAAAATCGCGTGGTTGGAGCTATGCAGCTGTATTCTGTAGA CAGGAAAGTCTCTCAGCCCATTGAAGGGCATGCGGCGAGTTTTGCACAATTTAAGATGGAAGGAAACGCA GAAGAATCGACGTTATTTTGTTTTGCTGTGCGGGGTCAGGCAGGGGGGAAGTTACATATCATTGAAGTTG GTACACCACCCACAGGAAACCAACCCTTTCCAAAGAAGGCAGTGGATGTCTTCTTTCCTCCAGAAGCACA AAATGATTTTCCTGTTGCAATGCAGATCAGTGAAAAGCATGACGTGGTGTTCCTGATTACCAAGTATGGT TATATTCACCTCTATGACCTTGAGACTGGCACGTGCATCTACATGAACAGAATCAGTGGAGAAACCATCT TTGTTACTGCTCCTCATGAAGCCACAGCTGGAATAATAGGAGTCAACAGAAAGGGACAAGTCCTATCAGT TTGTGTGGAAGAAGAGAACATAATTCCTTACATCACCAATGTCCTACAAAATCCTGATTTGGCTCTGAGA ATGGCTGTGCGGAACAACCTAGCTGGTGCAGAAGAGCTCTTCGCCCGGAAATTTAATGCTCTCTTCGCCC AGGGAAACTACTCAGAGGCTGCAAAGGTGGCTGCTAATGCACCGAAGGGAATCCTTCGTACTCCAGATAC CATCCGTCGATTCCAGAGTGTCCCAGCTCAGCCAGGTCAAACTTCACCTCTGCTTCAGTATTTTGGAATC CTTTTGGACCAAGGCCAGCTGAACAAATACGAGTCCTTAGAGTTATGTAGACCTGTCCTTCAGCAAGGGC GGAAACAACTTTTGGAGAAATGGCTGAAAGAAGATAAGCTGGAATGTTCTGAAGAACTGGGTGATCTCGT AAAATCTGTGGACCCAACGTTAGCACTGAGTGTATACCTCAGGGCCAATGTCCCTAATAAAGTCATCCAG TGCTTTGCAGAGACAGGTCAAGTCCAGAAGATTGTTTTATATGCTAAAAAAGTTGGCTATACTCCAGACT GGATCTTTCTCCTGAGAAATGTCATGCGGATCAGTCCAGATCAAGGACAGCAGTTTGCCCAAATGTTAGT TCAGGACGAAGAACCTCTTGCTGATATCACACAGATTGTGGATGTCTTTATGGAATACAATCTAATCCAA CAATGTACTGCCTTCTTACTTGATGCTTTGAAGAATAATCGCCCTTCTGAAGGTCCTTTGCAGACGCGAT TGCTTGAGATGAACCTTATGCACGCACCTCAAGTTGCAGATGCCATTCTAGGGAATCAGATGTTCACCCA CTATGACCGGGCTCACATCGCCCAGCTGTGTGAGAAGGCGGGCCTACTGCAGCGTGCATTAGAACATTTC ACCGACTTATATGATATAAAACGAGCAGTCGTTCATACCCATCTTCTCAACCCCGAGTGGTTAGTGAATT ACTTTGGGTCCTTATCAGTTGAAGATTCCCTAGAATGTCTCAGAGCCATGCTGTCTGCTAATATTCGTCA AAATCTACAGATCTGGGTTCAGGTCGCTTCTAAATATCATGAACAGCTGTCAACTCAGTCCCTGATTGAA CTCTTCGAGTCATTCAAGAGTTTTGAAGGCCTCTTTTATTTTCTGGGGTCCATTGTTAACTTCAGTCAAG ACCCAGATGTGCACTTTAAATATATTCAGGCGGCTTGCAAGACTGGACAAATCAAAGAGGTGGAAAGAAT CTGCAGAGAGAGCAACTGCTATGATCCAGAGCGCGTCAAGAATTTCCTCAAGGAGGCGAAACTCACAGAT CAGTTGCCACTCATCATCGTCTGTGACCGCTTTGACTTCGTCCATGACCTGGTGCTGTATTTATACAGAA ACAGTCTTCAGAAGTACATAGAGATATATGTCCAGAAAGTAAATCCAAGTCGACTTCCTGTGGTTATTGG AGGATTGCTGGATGTTGACTGCTCTGAAGATGTCATTAAAAATTTGATTCTTGTTGTGAGAGGTCAATTC TCTACTGATGAGCTTGTTGCTGAAGTTGAGAAAAGAAACAGATTGAAGCTGCTTCTTCCTTGGCTTGAGG CTAGAATTCATGAGGGCTGTGAGGAGCCTGCTACACACAATGCGTTAGCCAAAATATACATCGACAGCAA TAACAACCCAGAGAGATTCCTTCGTGAAAACCCCTACTATGACAGTCGTGTCGTGGGAAAGTACTGTGAG AAGAGAGACCCACATCTGGCCTGTGTTGCTTATGAACGTGGCCAGTGTGATCTGGAACTAATCAACGTTT GCAATGAGAATTCCCTCTTCAAAAGTCTTTCTCGCTACCTGGTCCGTCGAAAGGATCCAGAGTTGTGGGG TAGTGTGCTACTGGAAAGCAATCCATACAGAAGACCATTAATTGACCAGGTCGTACAGACAGCCTTGTCT GAAACTCAGGACCCAGAAGAAGTGTCAGTAACTGTCAAGGCTTTTATGACAGCAGATCTTCCTAATGAAC TCATTGAGCTGCTGGAGAAAATTGTCCTTGATAACTCTGTATTCAGTGAACACAGGAATCTGCAAAACCT CCTCATCCTCACTGCAATTAAGGCTGACCGAACCCGAGTTATGGAGTATATTAACCGCCTGGACAATTAT GATGCGCCAGATATTGCCAACATTGCCATCAGCAATGAGCTCTTCGAAGAAGCATTTGCTATTTTCCGGA AGTTTGATGTCAATACTTCAGCAGTGCAGGTGTTAATTGAACATATTGGAAACCTGGATCGTGCATATGA GTTTGCTGAACGCTGCAATGAGCCTGCTGTTTGGAGTCAACTCGCAAAAGCTCAGTTGCAGAAAGGGATG GTGAAGGAAGCCATTGATTCTTACATCAAAGCAGATGATCCTTCGTCTTACATGGAAGTTGTTCAGGCCG CCAATACTAGTGGAAACTGGGAAGAATTGGTGAAGTATTTGCAGATGGCACGCAAGAAGGCCCGAGAGTC CTACGTGGAGACAGAATTGATATTTGCACTGGCTAAAACAAACCGTCTTGCAGAATTAGAAGAGTTCATC AATGGGCCAAATAATGCTCATATTCAGCAAGTTGGTGACCGTTGTTATGATGAGAAAATGTATGATGCTG CTAAGTTGTTGTACAATAATGTTTCTAACTTTGGACGTTTGGCGTCTACCCTAGTTCACCTTGGCGAGTA TCAGGCAGCAGTTGATGGTGCTAGAAAAGCTAATAGTACTCGAACATGGAAAGAGGTCTGCTTTGCCTGT GTAGATGGGAAAGAGTTTCGCCTTGCTCAGATGTGTGGACTCCATATTGTTGTGCATGCAGATGAATTAG AGGAGCTTATCAACTACTATCAGGATCGTGGATATTTTGAAGAACTCATCACCATGTTGGAGGCAGCCTT GGGACTAGAGCGAGCTCACATGGGAATGTTCACAGAGCTAGCAATTTTGTATTCTAAATTCAAGCCACAG AAAATGAGGGAACACCTAGAGTTGTTTTGGTCAAGAGTGAATATTCCTAAGGTGCTGAGAGCCGCAGAGC AAGCCCATCTTTGGGCAGAGCTGGTGTTTCTGTATGACAAGTATGAAGAGTATGATAATGCCATCATCAC TATGATGAATCACCCCACCGACGCATGGAAGGAAGGGCAGTTCAAAGACATCATCACCAAGGTGGCTAAT GTGGAACTGTACTACAAAGCAATCCAGTTCTACTTAGAATTCAAGCCCTTGTTGTTAAATGACTTGCTCA TGGTGCTGTCTCCACGGTTGGCTCATACTCGTGCAGTCAATTATTTCAGCAAGGTTAAACAGCTACCACT GGTGAAACCATATTTACGCTCAGTTCAGAACCACAACAACAAGTCCGTGAACGAATCCCTGAACAACCTC TTTATCACAGAGGAGGATTACCAGGCTCTGCGAACATCAATAGATGCTTATGACAACTTTGACAACATCT CACTTGCTCAACGTTTGGAAAAACATGAACTCATTGAATTCAGAAGAATTGCTGCTTATCTCTTCAAAGG AAACAATCGCTGGAAACAGAGTGTGGAGCTGTGCAAGAAAGACAGCCTTTACAAGGATGCAATGCAGTAT GCCTCTGAATCGAAAGACACAGAATTGGCTGAAGAACTCCTGCAGTGGTTTCTGCAGGAAGAGAAGAGAG AATGCTTTGGAGCTTGTCTCTTCACCTGCTACGATCTTTTAAGGCCAGATGTTGTCCTGGAAACTGCATG GAGACATAATATCATGGATTTTGCCATGCCCTATTTCATCCAGGTCATGAAGGAGTATTTGACCAAGGTG GATAAATTAGATGCTTCGGAGTCACTGAGAAAAGAAGAAGAACAAGCTACAGAGACACAGCCCATTGTTT ATGGTCAGCCCCAGCTGATGCTAACAGCCGGACCCAGTGTGGCAGTCCCTCCCCAGGCACCTTTTGGTTA TGGTTATACTGCACCACCATATGGGCAGCCACAGCCTGGCTTTGGGTACAGCATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR207450 representing NM_019299 Red=Cloning site Green=Tags(s) MAQILPIRFQEHLQLQNLGINPANIGFSTLTMESDKFICIREKVGEQAQVVIIDMNDPSNPIRRPISADS AIMNPASKVIALKAGKTLQIFNIEMKSKMKAHTMTDDVTFWKWISLNTVALVTDNAVYHWSMEGESQPVK MFDRHSSLAGCQIINYRTDAKQKWLLLTGISAQQNRVVGAMQLYSVDRKVSQPIEGHAASFAQFKMEGNA EESTLFCFAVRGQAGGKLHIIEVGTPPTGNQPFPKKAVDVFFPPEAQNDFPVAMQISEKHDVVFLITKYG YIHLYDLETGTCIYMNRISGETIFVTAPHEATAGIIGVNRKGQVLSVCVEEENIIPYITNVLQNPDLALR MAVRNNLAGAEELFARKFNALFAQGNYSEAAKVAANAPKGILRTPDTIRRFQSVPAQPGQTSPLLQYFGI LLDQGQLNKYESLELCRPVLQQGRKQLLEKWLKEDKLECSEELGDLVKSVDPTLALSVYLRANVPNKVIQ CFAETGQVQKIVLYAKKVGYTPDWIFLLRNVMRISPDQGQQFAQMLVQDEEPLADITQIVDVFMEYNLIQ QCTAFLLDALKNNRPSEGPLQTRLLEMNLMHAPQVADAILGNQMFTHYDRAHIAQLCEKAGLLQRALEHF TDLYDIKRAVVHTHLLNPEWLVNYFGSLSVEDSLECLRAMLSANIRQNLQIWVQVASKYHEQLSTQSLIE LFESFKSFEGLFYFLGSIVNFSQDPDVHFKYIQAACKTGQIKEVERICRESNCYDPERVKNFLKEAKLTD QLPLIIVCDRFDFVHDLVLYLYRNSLQKYIEIYVQKVNPSRLPVVIGGLLDVDCSEDVIKNLILVVRGQF STDELVAEVEKRNRLKLLLPWLEARIHEGCEEPATHNALAKIYIDSNNNPERFLRENPYYDSRVVGKYCE KRDPHLACVAYERGQCDLELINVCNENSLFKSLSRYLVRRKDPELWGSVLLESNPYRRPLIDQVVQTALS ETQDPEEVSVTVKAFMTADLPNELIELLEKIVLDNSVFSEHRNLQNLLILTAIKADRTRVMEYINRLDNY DAPDIANIAISNELFEEAFAIFRKFDVNTSAVQVLIEHIGNLDRAYEFAERCNEPAVWSQLAKAQLQKGM VKEAIDSYIKADDPSSYMEVVQAANTSGNWEELVKYLQMARKKARESYVETELIFALAKTNRLAELEEFI NGPNNAHIQQVGDRCYDEKMYDAAKLLYNNVSNFGRLASTLVHLGEYQAAVDGARKANSTRTWKEVCFAC VDGKEFRLAQMCGLHIVVHADELEELINYYQDRGYFEELITMLEAALGLERAHMGMFTELAILYSKFKPQ KMREHLELFWSRVNIPKVLRAAEQAHLWAELVFLYDKYEEYDNAIITMMNHPTDAWKEGQFKDIITKVAN VELYYKAIQFYLEFKPLLLNDLLMVLSPRLAHTRAVNYFSKVKQLPLVKPYLRSVQNHNNKSVNESLNNL FITEEDYQALRTSIDAYDNFDNISLAQRLEKHELIEFRRIAAYLFKGNNRWKQSVELCKKDSLYKDAMQY ASESKDTELAEELLQWFLQEEKRECFGACLFTCYDLLRPDVVLETAWRHNIMDFAMPYFIQVMKEYLTKV DKLDASESLRKEEEQATETQPIVYGQPQLMLTAGPSVAVPPQAPFGYGYTAPPYGQPQPGFGYSM TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_019299
ORF Size	5025 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_019299.1, NP_062172.1
RefSeq Size	6071 bp
RefSeq ORF	5028 bp
Locus ID	54241
UniProt ID	P11442
MW	191.6 kDa
Gene Summary	heavy chain component of clathrin triskelions in coated vesicles [RGD, Feb 2006]

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