Gclm (NM_017305) Rat Tagged ORF Clone
CAT#: RR210532
- TrueORF®
Gclm (Myc-DDK-tagged ORF) - Rat glutamate cysteine ligase, modifier subunit (Gclm), (10 ug)
"NM_017305" in other vectors (3)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Glclr |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR210532 representing NM_017305
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCACCGACAGCCGCGCGGCCGGAGCACTTCTGGCGCGGGCCAGCACCCTGCACCTGCAGACCGGGA ACCTGCTCAACTGGGGCCGCCTGCGGAAAAAGTGTCCGTCCACGCACAGCGAGGAGCTTCGAGACTGTAT CCAAAAGACCTTGAATGAATGGAGCTCCCAAATCAGCCCTGATTTGGTCAGGGAGTTTCCAGATGTTTTG GAATGTACCATGTCCCATGCAGTGGAAAAGATAAACCCTGATGAAAGAGAAGAAATGAAAGTTTCTGCTA AACTGTTCATTGTAGGATCGAATTCTTCATCATCAACTAGAAATGCAGTTGACATGGCATGCTCAGTCCT TGGAGTTGCACAGCTGGACTCTGTCATCATGGCTTCCCCTCCAATTGAAGATGGAGTTAATCTTTCCTTG GAGCATTTGCAGCCTTACTGGGAGGAATTAGAAAACTTAGTTCAGAGCAAGAAGATTGTTGCTATAGGCA CCTCGGATCTAGACAAAACACAGTTGGAGCAGCTGTACCAGTGGGCACAGGTAAAACCCAATAGTAATCA AGTTAATCTTGCCTCCTGCTGTGTGATGCCACCAGATTTGACTGCATTTGCTAAACAGTTTGACATACAG CTACTGACTCACAATGACCCAAAAGAACTGCTCTCTGAGGCAAGTTTCCAGGAAGCTCTTCAAGAAAGCA TCCCTGACATTGAAGCCCAGGAGTGGGTGCCACTGTGGCTGCTGAGGTACTCGGTCATCGTGAAAAGCAG AGGAATCATCAAGTCAAAAGGATACATTTTGCAAGCCAAAAGAAAGGGTTCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR210532 representing NM_017305
Red=Cloning site Green=Tags(s) MGTDSRAAGALLARASTLHLQTGNLLNWGRLRKKCPSTHSEELRDCIQKTLNEWSSQISPDLVREFPDVL ECTMSHAVEKINPDEREEMKVSAKLFIVGSNSSSSTRNAVDMACSVLGVAQLDSVIMASPPIEDGVNLSL EHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQ LLTHNDPKELLSEASFQEALQESIPDIEAQEWVPLWLLRYSVIVKSRGIIKSKGYILQAKRKGS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_017305 |
ORF Size | 822 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_017305.2, NP_059001.1 |
RefSeq Size | 1524 bp |
RefSeq ORF | 825 bp |
Locus ID | 29739 |
UniProt ID | P48508 |
MW | 30.5 kDa |
Gene Summary | an integral part of the enzyme gamma-glutamylcysteine synthetase, which catalyzes the first step of glutathione (GSH) synthesis [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN210532 | Gclm (untagged ORF) - Rat glutamate cysteine ligase, modifier subunit (Gclm), (10 ug) |
CNY 3,990.00 |
|
RR210532L3 | Lenti ORF clone of Gclm (Myc-DDK-tagged ORF) - Rat glutamate cysteine ligase, modifier subunit (Gclm), (10 ug) |
CNY 6,080.00 |
|
RR210532L4 | Lenti ORF clone of Gclm (mGFP-tagged ORF) - Rat glutamate cysteine ligase, modifier subunit (Gclm), (10 ug) |
CNY 6,650.00 |