Sdhb (NM_001100539) Rat Tagged ORF Clone
CAT#: RR210540
- TrueORF®
Sdhb (Myc-DDK-tagged ORF) - Rat succinate dehydrogenase complex, subunit B, iron sulfur (Ip) (Sdhb), nuclear gene encoding mitochondrial protein, (10 ug)
"NM_001100539" in other vectors (3)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR210540 representing NM_001100539
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGTGGTCGGGGTCTCCTTGAAGCGCGGCTTTTCGGCTACGGCTCTCGGCAGAGTCGGCCTGC AGTTTCAGGCCTGCCGAGAGGCACAGACAGCTGCTGCTGCAGCTCCCAGAATCAAAACCTTCGCCATTTA CCGATGGGACCCGGACAAGGCTGGAGATAAACCTCGAATGCAGACATACAAGGTGGATCTGAATAAGTGT GGACCGATGGTGCTGGATGCTCTAATCAAGATTAAGAATGAAATCGATTCTACTTTGACCTTCCGAAGAT CATGCAGAGAAGGGATCTGTGGCTCTTGTGCCATGAACATCAACGGAGGCAACACGCTGGCATGCACGCG CAGGATCGACACAGACCTTGGCAAAGTCTCGAAAATATACCCTCTTCCGCATATGTATGTGATCAAGGAT CTAGTCCCTGACTTGAGTAACTTCTATGCACAATACAAATCCATTGAGCCCTATCTGAAGAAGAAGGATG AGTCCCAGGAGGGCAAGCAACAGTATCTGCAATCCATCGAGGACCGGGAGAAGCTGGACGGATTGTATGA GTGCATCCTGTGTGCCTGCTGCAGCACCAGCTGTCCCAGTTACTGGTGGAACGGAGACAAGTACCTGGGG CCTGCAGTTCTCATGCAGGCTTATCGCTGGATGATCGACTCCAGAGATGAATTCACAGAGGAGCGCCTGG CCAAGCTGCAGGACCCCTTCTCTCTCTACCGCTGCCACACCATCATGAACTGTACACAGACCTGCCCCAA GGGTCTGAATCCAGGAAAAGCAATTGCAGAAATCAAGAAGATGATGGCGACCTACAAGGAGAAGCGGGCA TTGGCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR210540 representing NM_001100539
Red=Cloning site Green=Tags(s) MAAVVGVSLKRGFSATALGRVGLQFQACREAQTAAAAAPRIKTFAIYRWDPDKAGDKPRMQTYKVDLNKC GPMVLDALIKIKNEIDSTLTFRRSCREGICGSCAMNINGGNTLACTRRIDTDLGKVSKIYPLPHMYVIKD LVPDLSNFYAQYKSIEPYLKKKDESQEGKQQYLQSIEDREKLDGLYECILCACCSTSCPSYWWNGDKYLG PAVLMQAYRWMIDSRDEFTEERLAKLQDPFSLYRCHTIMNCTQTCPKGLNPGKAIAEIKKMMATYKEKRA LA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001100539 |
ORF Size | 846 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001100539.1, NP_001094009.1 |
RefSeq Size | 1007 bp |
RefSeq ORF | 849 bp |
Locus ID | 298596 |
UniProt ID | P21913 |
MW | 31.8 kDa |
Gene Summary | Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN210540 | Sdhb (untagged ORF) - Rat succinate dehydrogenase complex, subunit B, iron sulfur (Ip) (Sdhb), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 3,990.00 |
|
RR210540L3 | Lenti ORF clone of Sdhb (Myc-DDK-tagged ORF) - Rat succinate dehydrogenase complex, subunit B, iron sulfur (Ip) (Sdhb), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 6,080.00 |
|
RR210540L4 | Lenti ORF clone of Sdhb (mGFP-tagged ORF) - Rat succinate dehydrogenase complex, subunit B, iron sulfur (Ip) (Sdhb), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 6,650.00 |