Myo9b (NM_012984) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR211834 representing NM_012984 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGCTCACGAGGCTGGCAGCTCAGGCCGAAGGCGGCCGGCCACCTTCCACCTGCACATCTACCCTC AGCTGCCCAGTGCTGGGAGCCAGACCTCATGCCGTGTAACCGCCACCAAGGACAGCACAACAAGCGATGT CATCCGAGACGTGGTGGCCAGTCTACACCTGGACGGCTCGAAGCACTATGTGCTGGTGGAGGTGAAGGAG TCGGGTGGGGAGGAGTGGGTGCTGGATGCCAGCGACTCGCCTGTGCACCGAGTGCTGCTGTGGCCTCGGC GAGCGCAGAAGGAGCACCCTCGGGAGGACGGCTACTACTTCCTGCTGCAGGAGCGCAACGCTGACGGCAG CATTCAGTACCTGCACGTGCAGCTGCTGGCTCAGCCCACAGCCGCATGTCGCCTGGTGGAGCGAGGGCTG CTGCCAAGGCCTCAAGCGGACTTCGACGACCTGTGCAACCTGCCGGAGCTGAACGAGGCCAACCTGCTGC AGAGCCTGAAGCTGCGCTTCGTGCAGCAGAAGATCTACACGTACGCGGGCAGCATCCTGGTGGCCATCAA CCCCTTCAAGTTCCTGCCCATTTACAACCCCAAGTATGTGAAGATGTACGAGAACCAGCAGCTGGGAAAG CTGGAGCCGCACGTGTTCGCTCTGGCCGATGTAGCCTACTACACCATGCTGCGCAAGCACGTGAACCAGT GCATCGTCATCTCTGGTGAGAGCGGGTCCGGCAAGACGCAAAGCACCAACTTCCTCATCCACTGCCTCAC AGCGCTCAGCCAGAAGGGCTACGCCAGCGGCGTCGAGAGGACCATCCTGGGTGCAGGGCCTGTGCTGGAG GCTTTTGGGAACGCCAAGACAGCCCACAACAACAACTCCAGCCGCTTCGGGAAGTTCATCCAAGTCAACT ACCTGGAGAGTGGCATCGTGAGGGGAGCTGTTGTGGAAAAATACCTTCTTGAAAAGTCTCGCCTGGTTTC CCAGGAGAAGGATGAGCGGAACTACCATGTGTTTTATTATCTGCTGCTGGGCGTCAGTGAGGAAGAGCGT CAGGAATTTCAGCTGAAGCAGCCTCAAGACTATTTCTACCTCAACCAGCATAACTTGAATATTGAAGATG GAGAAGACCTCAAACATGACTTTGAAAGGCTTCAGCAGGCCATGGAGATGGTGGGCTTCCTACCTGCCAC GAAGAAGCAGATCTTCTCTGTCCTCTCAGCCATCCTGTATCTTGGCAATGTCACCTATAAGAAGAGAGCC ACAGGCCGGGATGAAGGCCTGGAGGTCGGGCCCCCGGAGGTATTGGACACACTATCCCAGCTCCTGAAGG TAAAGCGGGAGACCCTGGTGGAGGTCCTAACCAAGAGAAAAACAATCACGGTCAATGACAAACTCATCCT GCCTTACAGCCTCAGTGAGGCTATCACTGCACGAGACTCCATGGCTAAGTCTCTGTACAGCGCCCTGTTT GACTGGATTGTGCTGAGGATCAACCACGCCCTCCTCAACAAGAAGGACATGGAAGAGGCTGTTTCTTGCT TGTCCATTGGCGTCCTGGACATATTCGGATTTGAGGACTTTGAACGGAACAGCTTCGAGCAGTTTTGCAT CAACTACGCCAACGAGCAGTTGCAGTACTACTTCACCCAGCACATCTTCAAGCTGGAGCAGGAGGAGTAC CAGGGTGAGGGTATCTCGTGGCACAACATTGACTACACCGACAACGTGGGCTGTATCCACCTCATCAGCA AGAAGCCCACTGGCCTCTTCTACCTGCTGGACGAGGAAAGCAACTTTCCCCATGCCACAAGCCACACTCT GCTGGCCAAATTCAAGCAGCAGCATGAGGACAACAAGTACTTCCTGGGCACACCAGTCCTGGAGCCCGCC TTCATCATCCAGCACTTCGCAGGCAGAGTAAAGTACCAGATCAAGGACTTCCGGGAGAAGAACATGGACT ACATGCGGCCTGACATCGTGGCACTGCTAAGGGGCAGTGACAGCTCCTATGTGCGCCAACTCATCGGCAT GGACCCGGTAGCTGTGTTCCGCTGGGCTGTATTACGGGCAGCCATCAGGGCCATGGCTGTGCTGCGGGAG GCTGGGCGCCTGCGTGCAGAGAGAGCAGAGAAAGCAGAAGCAGGTGTAAGTAGTCCTGTCACTCGAAGTC ACGTGGAAGAGCTGCCAAGAGGAGCCAACACCCCTTCAGAGAAACTGTACCGCGATTTGCATAACCAAAT CATCAAGAGCCTCAAAGGACTGCCATGGCAGGGCGAGGACCCGCGGAGGCTTCTCCAGTCCCTCAGTCGG CTCCAGAAGCCCCGCACCTTCTTCCTGAAGAGTAAAGGTATCAAACAAAAGCAGATCATTCCCAAGAACC TGCTGGACTCTAAGTCCCTGAGGCTCATCATCAGCATGACGCTGCATGACCGAACTACCAAGTCACTGCT GCACCTGCACAAGAAGAAGAAGCCGCCCAGCATCAGTGCACAGTTCCAGACATCTCTTAACAAGCTGTTG GAGGCGCTGGGCAAGGCTGAGCCCTTCTTCATCCGCTGCATTCGCTCCAATGCCGAGAAGAAGGAGCTCT GCTTTGATGATGAGCTGGTGCTTCAGCAACTGCGCTACACAGGCATGCTGGAGACCGTGCGCATCCGGCG CTCTGGCTACAGCGCCAAGTACACCTTCCAGGACTTCACGGAGCAGTTCCAGGTGCTGCTGCCCAAGGAT GTCCAGCCCTGTAGGGAGGCCATTGCTGCCCTGTTGGAGAAGCTGCAGGTGGACAGGCAGAACTACCAGA TCGGGAAGACGAAGGTCTTCCTGAAGGAGACAGAGCGGCAGGCCCTGCAGGAGAGGCTGCATGGTGAGGT CTTACGCAGGATCCTGCTGCTGCAGAGTTGGTTCCGGATGGTGCTGGAACGCAGGCACTTTGTGCAGATG AAGCATGCTGCCCTGACCATCCAGGCCTGCTGGCGGTCTTATCGTGTGCGCCGTACACTGGAGAGGACGC GGGCAGCTGTGTATCTTCAGGCTGCCTGGAGAGGCTACCTGCAGAGACAGGCCTACCACCACCAGAGGCA TAGCATCATCCGCCTGCAGAGCCTCTGCCGTGGCCACCTACAGCGCAGGAGCTTCAGCCAGATGATGTTA GAGAAGCAGAAGGCAGAGCAAGCCAGGGAGACTGCAGGAGCAGAGATGTCAGAGGGAGAGCCCAGCCCTG TGGCCGCTGGGGAGCAGCCGTCTGAGCACCCTGTGGAGGACCCTGAGAGCCTGGGTGTGGAGACTGAGAC CTGGATGAACAGCAAGTCCCCAAATGGCTTGTCACCTAAGAAGGAGATCCCCAGCCCGGAGATGGAGACC CCAGCCCAAAAAACAGTGCCAGCTGAAAGTCATGAGAAAGTCCCCAGTAGCCGGGAGAAGCGAGAGTCAC GGCGGCAGCGAGGGTTGGAGCATGTTGAACGACAGAACAAACACATCCAATCCTGCAGGGAGGAGAATAG CACCCTCCGAGAACCTTCCAGAAAGGCAAGCCTGGAAACAGGGGAGAGCTTCCCTGAGGACACAAAGGAG CCCAGAGAAGATGGACTTGAGACATGGACTGAGACCGCAGCCCCCTCTTGTCCAAAGCAGGTGCCGATTG TGGGAGATCCGCCTAGGAGTCCCAGTCCCTTGCAGAGGCCCGCCAGCCTGGACCTAGACAGCAGGGTTAG CCCAGTGCTCCCCAGCAGCTCCCTGGAATCCCCCCAGGATGAGGACAAGGGTGAGAACAGCACCAAGGTT CAGGACAAGCCCGAGAGTCCCAGTGGCTCCACCCAGATCCAACGGTACCAACACCCGGACACAGAGCGGC TAGCCACTGCTGTGGAGATATGGCGAGGCAAGAAGCTCGCCAGTGCCATGCTGAGCCAATCCCTGGACCT GAGTGAGAAGCCCCGGACTGCAGGGGCAGCCCTGACTCCCACAGAGGAGAGGCGCATCTCCTTCTCCACC AGTGATGTCTCCAAGCTGTCCCCGGTCAAGACTTCAACTGAAGTCGATGGGGATCTGAGCGCCAAGAAGC CAGCCGGCCATAAGAAGAAGTCAGAAGACCCATCTGCTGGTCCCGATGCAGGCCTGCCCACAGGCTCCCA GGGTGACTCTAAATCTGCCTTTAAGCGACTCTTCCTGCACAAAGCCAAGGATAAGAAGCCCAGCCTGGAG GGTGTGGAGGAGACAGAGGGCAGTGGAGGGCAGGCTGCACAGGAGGCCCCGGCCAGGAAGACTCTAGATG TACCTTCTAGCCAGCAGCACCGCCACACCACAGGCGAGAAGCCTCTAAAAGGGAAGAAGAACCGAAATCG TAAGGTCGGACAGATCACAGTGTCCGAGAAGTGGCGAGAGTCGGTGTTCCGTAAGATCACTAATGCCAAC GAGCTCAAGTTTCTGGATGAGTTCCTGCTCAACAAGGTGAATGACCTTCGCTCACAGAAGACACCCATCG AGAGCTTGTTCATTGAGGCCACTGAGCGTTTCAGGAGCAATATCAAGACCATGTATTCTGTGCCTAACGG GAAGATCCATGTAGGCTACAAGGACTTGATGGAGAACTACCAGATCGTTGTCAGTAACCTGGCTGCCGAG CGTGGGGAGAAGGACACCAACCTGGTCCTCAATGTCTTCCAGTCACTGCTGGATGAATTCACTCGCAGCT ACAACAAGACTGACTTTGAGCCGGTCAAGGGCAAAGCCCAGAAGAAGAAGAGGAAGCAGGAGCGTGCTGT CCAGGAACACAATGGACATGTGTTTGCCAGCTACCAAGTGAACATCCCGCAGTCGTGTGAGCAGTGTCTG TCCTACATCTGGCTCATGGACAAGGCCCTGCTATGCAGTGTGTGCAAGATGACCTGCCACAAGAAATGTG TGCACAAGATTCAGAGCTATTGCTCCTACACTGGACGGAGGAAGAGTGAGCTGGGTGCCGAACCAGGCCA CTTCGGCGTGTGTGTAGACAGCCTGACCAGTGACAAGGCCTCCGTGCCCATTGTGCTGGAGAAGCTTCTG GAACATGTAGAGATGCATGGCCTGTATACCGAGGGTCTTTACCGCAAGTCAGGCGCTGCCAACCGGACAC GGGAATTACGCCAGGCTCTGCAGACAGACCCTGCTACAGTTAAGCTGGAGGACTTCCCCATCCACGCCAT CACCGGGGTCCTGAAGCAATGGCTGCGGGAGCTGCCTGAGCCACTCATGACTTTTGCCCAGTATGGAGAC TTCCTCAGGGCTGTTGAGCTTCCAGAGAAGCAGGAGCAGCTGGCTGCCATCTATGCAGTCCTGGACCACC TGCCTGAAGCCAACCACACCTCCCTGGAGAGGCTCATCTTCCACCTTGTCAAAGTGGCCCTGCTTGAAGA TGTGAACCGCATGTCTCCGGGAGCTCTAGCTATCATCTTCGCGCCCTGCCTGCTTCGCTGCCCTGACAAC TCCGACCCCCTGACCAGCATGAAGGACGTACTAAAGATTACCACGTGTGTGGAGATGCTCATCAAGGAAC AGATGAGGAAATACAAGGTGAAGATGGAGGAGATCAACCACCTGGAGGCAGCTGAGAGCATTGCGTTCCG CAGGCTCTCCCTGCTGAGGCAGAATGCCCCGTGGCCTCTCAAACTGGGGTTTTCGTCTCCCTATGAGGGG GTCCGGACCAAAAGCCCCAGGACCCCAGTGGTCCAAGACTTGGAGGAGCTGGGGGCTCTCCCCGAGGAGG CGGCAGGTGGTGACGAGGACCGGGAAAAGGAGATTCTCATGGAGAGGATTCAGTCCATCAAAGAAGAGAA GGAGGACATCACATATCGACTGCCGGAGCTGGACCCACGGGGTTCTGACGAGGAGAACCTTGACTCAGAG ACATCAGCCAGCACTGAGAGCCTGCTGGAGGAGAGGGCCGTGCGGGGGGCCGCAGAAGGTCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR211834 representing NM_012984 Red=Cloning site Green=Tags(s) MSAHEAGSSGRRRPATFHLHIYPQLPSAGSQTSCRVTATKDSTTSDVIRDVVASLHLDGSKHYVLVEVKE SGGEEWVLDASDSPVHRVLLWPRRAQKEHPREDGYYFLLQERNADGSIQYLHVQLLAQPTAACRLVERGL LPRPQADFDDLCNLPELNEANLLQSLKLRFVQQKIYTYAGSILVAINPFKFLPIYNPKYVKMYENQQLGK LEPHVFALADVAYYTMLRKHVNQCIVISGESGSGKTQSTNFLIHCLTALSQKGYASGVERTILGAGPVLE AFGNAKTAHNNNSSRFGKFIQVNYLESGIVRGAVVEKYLLEKSRLVSQEKDERNYHVFYYLLLGVSEEER QEFQLKQPQDYFYLNQHNLNIEDGEDLKHDFERLQQAMEMVGFLPATKKQIFSVLSAILYLGNVTYKKRA TGRDEGLEVGPPEVLDTLSQLLKVKRETLVEVLTKRKTITVNDKLILPYSLSEAITARDSMAKSLYSALF DWIVLRINHALLNKKDMEEAVSCLSIGVLDIFGFEDFERNSFEQFCINYANEQLQYYFTQHIFKLEQEEY QGEGISWHNIDYTDNVGCIHLISKKPTGLFYLLDEESNFPHATSHTLLAKFKQQHEDNKYFLGTPVLEPA FIIQHFAGRVKYQIKDFREKNMDYMRPDIVALLRGSDSSYVRQLIGMDPVAVFRWAVLRAAIRAMAVLRE AGRLRAERAEKAEAGVSSPVTRSHVEELPRGANTPSEKLYRDLHNQIIKSLKGLPWQGEDPRRLLQSLSR LQKPRTFFLKSKGIKQKQIIPKNLLDSKSLRLIISMTLHDRTTKSLLHLHKKKKPPSISAQFQTSLNKLL EALGKAEPFFIRCIRSNAEKKELCFDDELVLQQLRYTGMLETVRIRRSGYSAKYTFQDFTEQFQVLLPKD VQPCREAIAALLEKLQVDRQNYQIGKTKVFLKETERQALQERLHGEVLRRILLLQSWFRMVLERRHFVQM KHAALTIQACWRSYRVRRTLERTRAAVYLQAAWRGYLQRQAYHHQRHSIIRLQSLCRGHLQRRSFSQMML EKQKAEQARETAGAEMSEGEPSPVAAGEQPSEHPVEDPESLGVETETWMNSKSPNGLSPKKEIPSPEMET PAQKTVPAESHEKVPSSREKRESRRQRGLEHVERQNKHIQSCREENSTLREPSRKASLETGESFPEDTKE PREDGLETWTETAAPSCPKQVPIVGDPPRSPSPLQRPASLDLDSRVSPVLPSSSLESPQDEDKGENSTKV QDKPESPSGSTQIQRYQHPDTERLATAVEIWRGKKLASAMLSQSLDLSEKPRTAGAALTPTEERRISFST SDVSKLSPVKTSTEVDGDLSAKKPAGHKKKSEDPSAGPDAGLPTGSQGDSKSAFKRLFLHKAKDKKPSLE GVEETEGSGGQAAQEAPARKTLDVPSSQQHRHTTGEKPLKGKKNRNRKVGQITVSEKWRESVFRKITNAN ELKFLDEFLLNKVNDLRSQKTPIESLFIEATERFRSNIKTMYSVPNGKIHVGYKDLMENYQIVVSNLAAE RGEKDTNLVLNVFQSLLDEFTRSYNKTDFEPVKGKAQKKKRKQERAVQEHNGHVFASYQVNIPQSCEQCL SYIWLMDKALLCSVCKMTCHKKCVHKIQSYCSYTGRRKSELGAEPGHFGVCVDSLTSDKASVPIVLEKLL EHVEMHGLYTEGLYRKSGAANRTRELRQALQTDPATVKLEDFPIHAITGVLKQWLRELPEPLMTFAQYGD FLRAVELPEKQEQLAAIYAVLDHLPEANHTSLERLIFHLVKVALLEDVNRMSPGALAIIFAPCLLRCPDN SDPLTSMKDVLKITTCVEMLIKEQMRKYKVKMEEINHLEAAESIAFRRLSLLRQNAPWPLKLGFSSPYEG VRTKSPRTPVVQDLEELGALPEEAAGGDEDREKEILMERIQSIKEEKEDITYRLPELDPRGSDEENLDSE TSASTESLLEERAVRGAAEGH TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_012984
ORF Size	5943 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_012984.3, NP_037116.2
RefSeq Size	6987 bp
RefSeq ORF	5946 bp
Locus ID	25486
MW	225.1 kDa
Gene Summary	displays ATP-dependent binding to actin: binds members of the rho family of GTPases; may play a role in formation of the actin cytoskeleton [RGD, Feb 2006]

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