Macc1 (NM_001191775) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	7a5; RGD1309838
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217262 representing NM_001191775 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTAATCAGTGAAAGAGCCTATTATTGGCCACAAGGAATTGGTCGAAGTAGATCTGAAGGGAATTTGA TTGACATGGAAGCCTCGAAACTCTCAAAACATCACAATATTACAGCGTGGCAAGGGCGAGGCTTGCCTTA CAACTGGCCAGATGCTTCTACTCTCCATGGTGATAGAGATGCCAAAGCTACAAATCCATTCTGGAGTGAA CTGTCTGCCTCAAACCCATTTCTGGAGGACATCACGCAGCTAAGAAATAACAAAAAGAAAGATAACATGT CTATATTGAAGGAAGATCCTTTCCTTTTTTTAAGACAAATAGAAATAGGAAATTCTTTTGATTCCTCTGG TGATGAACTAGATGTGGATCAGGGGCTTAGGTCATCTTCCTCAAGGACGTCAGGAAGGTCTAAGAGTGTT TCAGAGCTCCTGGATGCCTTAGGTGGTACAACACACACTCACCACAGTATACAAAGCTCTGATCAGATCC TAGTACAAGACTTAGAATGGCTTCAAAACGACCGGGAGGCATATAAAATGGCTTGGCTAAGTCAACGTCA GCTGGCTCGCTCTTGCTTAGATCTGAATACAATTAATCAGAGTCCTGGGTGGGCTCAAACACAAACTGCA GAGACCATTGCAGTTTGTACATTAAATCACCAAGGAGGGTCAGTTCAGTTACCTGACTCAGACATCACTG TTCATGTACCTCAAGGACACGTAGCTATCGGAGAATTCCAGGAGGTATCTCTGAGAGCATTCCTGGATCC TCCACCAAAGCTGAACCATGATCTCTCCTGTACTATAAGCCCTCTCTTGGAAATCAGGTTAAGCAACCTG AATACCATGGAAGCTATTTTGTTGGAGATGAAAACTGGAACAGAAGTGAAAACAGATCCTCTGAGCCAAG TCATGACAAAAATAGTGTGTTTACACAGCCTGGACAGAGAAGGCCCTTTCCAAGAGCTAAACAACTGCTA TGTCTACAAAGATACCATCCAAGCTAAGCTTCTTGATCTGAGCCACGTAATGTATATAGTCATAGCTGCA CAAGCTACGATCATTCAGCCACCAGCTGCTACTATTTGGGATTATCTCCACAAAACCACCTCCATTGGAA TTTATGGACCAAAACATCTCCATCCAAGCTTTACTGCTGTTTTCACTGTTTGTGGACACAACTATGTGCC AGGAAAGTTTACAGTCTCCGACATTAGGAAGGGTGGAAAAAACACATCTCCAGTCGTATTTCACCTCTGG GGGAAGCATTCGTTTTCACTTGACAAGCCACAAGATTTAATTATTTCAGCTTTTTCATGTGATCCTGACG TAAAGGAAGAGGGAGAAAGAAAAGTAATTGCTCAAGGGCAATTGGAAGCAGGTGCGGTAGTCCATCAACA AGTTTTCTTTTCTTCAGTTGGCCACAGAGAGATGCACTTGTTTGTTTTCCGGGTTCAGGTAAAGGCCCCC AGCGGAAAACCAGTCACACAGTTCTTTGTCACTTCACCTGATCCAGCCCCAAATCTAAAAAGTTTCTCAT ATCTGCCAGACATTTTCCAGAAGAAGAGAGAAGATAGGAGAGCGTCTTTATTGTCATCAGGACTTGTTAA GTATCCCACATTCCAAAATAAAAACTTGAACTTTACCAGTTATGGGGTCACACTGAAGACAGTACTGAGG CAAAGTAAGATTGACTATTTACTTGAGTATTTAAAGGGGGACACAATTGCCCTCCTTGGCGAAGGTACGG TAAGGGCCATCGGACAGTCCAAAGTGAAAGAATGGTACGTAGGAGTCCTCCGAGGTAAGATCGGACTTGT GCATTGCAAAAATGTCAAGGTGATTTCCAAAGAACAAGTGATGTCTATGTCTGATGGCATTTTGACAACC AAGAATCTTCTGGAACAAATTGCCTTGCCATTTAAAAATCTGACTTACGTATACTCCGTTGTATTGACTT TGGTGTCAGAAAAAGTTGATGACTGGCAAGTTTTAGCTAATGTCCTGGGTTACTCAAATCTGGCACAGGA AGATTTTGATAGAATTCAAGCTGACAAGGAACCAGAAAGGGTTTCTTACATTGTCAAGAAGTTAAAGGAA GATTGCCATGAAGATAAAAATACCAGGAAGTTTCTTTATGAACTGATTGTGGCTCTGCTAAAAATGGACT GCCAAGGATTAGTGGCACGTCTTATCCAAGAGGCTGTTATTTTGACGTCAGCTGTAAAGCTTGGAAGAGG CTGGAGGGAACTTGCTGAGAAGTCTGCAGGACTCACTAAGCATCAGATGCAGGCATATGAAAATCCTCAC AGAGGAAAATCTGGAGAAGTTTCAGCAGAGACAATGTGGAAACCTGCCTATGATTTTCTCTATGCGTGGA GTTATCACTATGGAAATAGCTACAGAGATGTGTTACAAGACCTTCAATCAGCTTTGGACAGAATGAAAAA CCCTGTCACTAAACGGTGGAGAGATTTAACTGGAATCTTAATACTTGTAAATTCTTTAGAGATTTTGAGA GTAACTGCTTTCTCTACTTCTCAGGAAATA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217262 representing NM_001191775 Red=Cloning site Green=Tags(s) MLISERAYYWPQGIGRSRSEGNLIDMEASKLSKHHNITAWQGRGLPYNWPDASTLHGDRDAKATNPFWSE LSASNPFLEDITQLRNNKKKDNMSILKEDPFLFLRQIEIGNSFDSSGDELDVDQGLRSSSSRTSGRSKSV SELLDALGGTTHTHHSIQSSDQILVQDLEWLQNDREAYKMAWLSQRQLARSCLDLNTINQSPGWAQTQTA ETIAVCTLNHQGGSVQLPDSDITVHVPQGHVAIGEFQEVSLRAFLDPPPKLNHDLSCTISPLLEIRLSNL NTMEAILLEMKTGTEVKTDPLSQVMTKIVCLHSLDREGPFQELNNCYVYKDTIQAKLLDLSHVMYIVIAA QATIIQPPAATIWDYLHKTTSIGIYGPKHLHPSFTAVFTVCGHNYVPGKFTVSDIRKGGKNTSPVVFHLW GKHSFSLDKPQDLIISAFSCDPDVKEEGERKVIAQGQLEAGAVVHQQVFFSSVGHREMHLFVFRVQVKAP SGKPVTQFFVTSPDPAPNLKSFSYLPDIFQKKREDRRASLLSSGLVKYPTFQNKNLNFTSYGVTLKTVLR QSKIDYLLEYLKGDTIALLGEGTVRAIGQSKVKEWYVGVLRGKIGLVHCKNVKVISKEQVMSMSDGILTT KNLLEQIALPFKNLTYVYSVVLTLVSEKVDDWQVLANVLGYSNLAQEDFDRIQADKEPERVSYIVKKLKE DCHEDKNTRKFLYELIVALLKMDCQGLVARLIQEAVILTSAVKLGRGWRELAEKSAGLTKHQMQAYENPH RGKSGEVSAETMWKPAYDFLYAWSYHYGNSYRDVLQDLQSALDRMKNPVTKRWRDLTGILILVNSLEILR VTAFSTSQEI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191775
ORF Size	2550 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191775.1, NP_001178704.1
RefSeq Size	2792 bp
RefSeq ORF	2553 bp
Locus ID	314539
MW	95.7 kDa

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