Mcm4 (NM_033651) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Mcmd4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217274 representing NM_033651 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGTCCCCGGCATCCACCCCAAGTCGCCGCAGCAGCCGACGCGGACGAGTCACCCCAACCCAGTCGC TTCGAAGTGAGGAAAGCCGATCATCTCCCAGTGGGAGACGTAGAGGCGAAGATTCTTCCACCGGAGAGTT GCTGCCAATGCCCACCTCACCAGGAGCAGACCTGCAGAGCCCAGCTGCACAGAATGCCTTGTTTTCCAGC CCTCCTCAGATGCATTCTTCAGCTATTCCTTTGGAATTTGATGTTAGTTCGCCATTGACATATGGCACTC CCAGCTCTCGAGTGGAAGGAACCCCAAGAAGTGGGGTGAGAGGCACACCCATGAGGCAGAGGCCAGACCT GGGCTCAGCACGAAAGGGTTTGCAGGTGGATCTGCAGTCTGACGGAGCAGCAGCAGAAGACACAGTAGCA AGTGAACAGTCTCTAGGCCAAAAGCTTGTGATTTGGGGAACAGACGTGAATGTGGCAACATGCAAAGAAC ATTTTCAGAGATTCCTTCAATGTTTTACTGATCCTCTGGCCAAAGAAGAAGAAAATGTTGGCATAGATAT TACGCAACCTTTGTACATGCAACGACTTGGAGAGATTAATATTACAGGAGAGCCATTTTTAAATGTGAAC TGTGAACACATAAAATCATTTGGCAAAAACTTGTATAGACAGCTCATCTCCTACCCACAGGAGGTTATAC CAACCTTTGACATGGCTGTCAATGAGATCTTCTTTGACCGTTATCCTGACTCCATCTTAGAACATCAGAT TCAAGTCAGACCTTTTAATGCATTGAAGACAAAGAGTATGAGAAACTTGAATCCAGAAGACATTGATCAG CTCATCACCATCAGTGGCATGGTTATCAGAACGTCACAGCTGATTCCCGAGATGCAGGAGGCCTTTTTCC AGTGCCAAGTCTGTGCCCATACCACCCGGGTAGAGATGGACCGAGGCCGAATTGCTGAGCCCTGCACTTG CGTACACTGCCACACTACCCACAGTATGGCACTGATCCACAACCGTTCACTATTCTCTGACAAGCAGATG ATCAAGCTTCAAGAGTCTCCTGAAGATATGCCTGCTGGGCAGACACCTCACACTGTTGTCCTTTTTGCCC ACAATGATCTTGTAGACAAGGTTCAGCCAGGGGACAGAGTGAATGTCACAGGCATATATCGAGCAGTACC TATTCGAGTTAATCCAAGAGTGAGCAACGTGAAGTCTGTCTATAAAACCCACATTGATGTCATCCATTAT CGGAAAACGGATGCAAAACGTCTGCATGGCCTTGATGAAGAAGCAGAACAGAAACTTTTTTCAGAGAAAC GTGTGAAATTGCTTAAGGAACTTTCCAGGAAGCCAGATATTTATGAGCGGCTTGCTTCAGCCTTGGCTCC CAGCATTTATGAACATGAAGATATAAAAAAGGGAATCCTACTTCAACTCTTTGGTGGAACAAGGAAGGAT TTCAGTCACACTGGAAGGGGTAAATTCCGTGCTGAGATTAACATCCTTCTGTGTGGTGACCCTGGCACCA GCAAGTCCCAGCTGCTACAGTATGTATACAACCTGGTTCCCAGAGGCCAGTACACATCTGGAAAAGGCTC CAGTGCAGTTGGCCTCACAGCCTATGTGATGAAAGACCCTGAGACCAGGCAGCTTGTCCTCCAGACAGGT GCCCTTGTACTGAGTGACAATGGCATATGTTGCATTGATGAGTTTGACAAAATGAATGAAAGCACAAGGT CCGTGCTGCATGAGGTCATGGAACAGCAGACTCTGTCCATTGCAAAGGCTGGGATCATCTGTCAGCTCAA TGCACGAACCTCTGTCCTGGCAGCAGCAAACCCTATTGAGTCCCAGTGGAACCCTAAAAAAACAACCATT GAAAATATCCAACTACCACACACACTGTTGTCAAGGTTTGATCTCATTTTCCTCATGCTAGACCCTCAAG ATGAGGCATATGACCGACGCCTAGCCCACCACCTGGTTTCATTGTACTACCAAAGTGAGGAGCAGGTGGA GCAGGAGTTCCTGGACATGGCAGTGCTGAAAGACTACATTGCCTATGCACATAGTACTATCATGCCCCGG CTGAGTGAGGAGGCCAGCCAGGCTCTCATTGAGGCTTATGTAAACATGAGGAAGATCGGGAGTAGTCGGG GGATGGTTTCTGCTTACCCTCGACAGCTAGAGTCATTAATTCGCTTAGCAGAAGCCCATGCTAAAGTAAG ATTTTCAGACAAAGTTGAAGCAATCGATGTGGAAGAGGCAAAACGCCTTCACCGGGAGGCTCTGAAGCAG TCTGCCACTGACCCTCGCACTGGCATTGTGGACATATCTATTCTTACTACAGGAATGAGTGCCACTTCTC GTAAACGGAAAGAAGAATTAGCTGAAGCACTGAGAAAACTTATTTTATCTAAGGGTAAAACACCAGCCTT AAAGTACCAACAGCTATTTGAGGATATTCGGGGACAATCTGACACAGCAATTACCAAGGACATGTTTGAA GAGGCCCTGCGTGCTTTGGCTGATGATGATTTCCTAACAGTGACTGGGAAGACTGTCCGCCTACTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217274 representing NM_033651 Red=Cloning site Green=Tags(s) MSSPASTPSRRSSRRGRVTPTQSLRSEESRSSPSGRRRGEDSSTGELLPMPTSPGADLQSPAAQNALFSS PPQMHSSAIPLEFDVSSPLTYGTPSSRVEGTPRSGVRGTPMRQRPDLGSARKGLQVDLQSDGAAAEDTVA SEQSLGQKLVIWGTDVNVATCKEHFQRFLQCFTDPLAKEEENVGIDITQPLYMQRLGEINITGEPFLNVN CEHIKSFGKNLYRQLISYPQEVIPTFDMAVNEIFFDRYPDSILEHQIQVRPFNALKTKSMRNLNPEDIDQ LITISGMVIRTSQLIPEMQEAFFQCQVCAHTTRVEMDRGRIAEPCTCVHCHTTHSMALIHNRSLFSDKQM IKLQESPEDMPAGQTPHTVVLFAHNDLVDKVQPGDRVNVTGIYRAVPIRVNPRVSNVKSVYKTHIDVIHY RKTDAKRLHGLDEEAEQKLFSEKRVKLLKELSRKPDIYERLASALAPSIYEHEDIKKGILLQLFGGTRKD FSHTGRGKFRAEINILLCGDPGTSKSQLLQYVYNLVPRGQYTSGKGSSAVGLTAYVMKDPETRQLVLQTG ALVLSDNGICCIDEFDKMNESTRSVLHEVMEQQTLSIAKAGIICQLNARTSVLAAANPIESQWNPKKTTI ENIQLPHTLLSRFDLIFLMLDPQDEAYDRRLAHHLVSLYYQSEEQVEQEFLDMAVLKDYIAYAHSTIMPR LSEEASQALIEAYVNMRKIGSSRGMVSAYPRQLESLIRLAEAHAKVRFSDKVEAIDVEEAKRLHREALKQ SATDPRTGIVDISILTTGMSATSRKRKEELAEALRKLILSKGKTPALKYQQLFEDIRGQSDTAITKDMFE EALRALADDDFLTVTGKTVRLL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_033651
ORF Size	2586 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_033651.1, NP_387500.1
RefSeq Size	3339 bp
RefSeq ORF	2589 bp
Locus ID	29728
MW	96.6 kDa
Gene Summary	replication licensing factor; homolog of yeast CDC21 [RGD, Feb 2006]

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