Atp4a (NM_012509) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Hka; Hkatpc
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217427 representing NM_012509 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAAGGCAGAGAATTATGAATTGTACTCAGTGGAACTGGGGACTGGCCCTGGTGGGAACATGGCTG CCAAGATGAGCAAGAAGAAGGCGGGAGGCGGGGGAGGCAAGAAGAAGGAGAAGCTAGAGAACATGAAGAA GGAGATGGAGATGAACGACCACCAGTTGTCAGTGTCTGAGCTGGAGCAGAAGTACCAGACCAGTGCCACC AAGGGCCTGAAGGCGAGTCTGGCAGCTGAGCTGCTGCTGAGGGATGGGCCCAATGCACTCCGGCCACCTC GGGGCACCCCTGAGTACGTGAAGTTCGCCCGGCAGCTGGCAGGCGGTCTGCAGTGCCTCATGTGGGTGGC TGCAGCCATCTGCCTCATTGCCTTTGCGATTCAGGCCAGCGAGGGAGACCTGACCACTGATGACAATTTG TACCTGGCGTTGGCACTCATTGCTGTGGTTGTGGTCACTGGCTGTTTTGGCTACTACCAGGAGTTCAAGA GCACAAATATCATCGCCAGCTTCAAGAATCTTGTACCCCAGCAAGCCACAGTGATCCGAGACGGGGATAA GTTCCAGATCAACGCGGATCAGCTTGTGGTGGGCGACCTGGTAGAGATGAAAGGCGGGGACCGCGTCCCA GCAGACATCCGAATTCTGTCAGCCCAGGGCTGCAAGGTGGACAACTCCTCGCTTACTGGAGAGTCTGAAC CGCAGACCCGCTCACCTGAGTGTACACACGAGAGTCCCCTTGAGACCCGCAACATCGCCTTCTTCTCCAC CATGTGTCTGGAGGGCACGGCGCAGGGCTTGGTGGTGAGCACCGGTGATCGCACCATCATTGGACGCATC GCCTCTCTGGCTTCGGGTGTGGAAAACGAGAAGACTCCTATCGCGATCGAGATCGAACATTTTGTGGACA TCATTGCTGGCCTGGCCATCCTCTTCGGTGCCACATTCTTTGTGGTGGCCATGTGTATCGGCTATACCTT CCTTCGGGCCATGGTCTTCTTCATGGCCATTGTGGTAGCCTATGTGCCTGAGGGGCTGCTGGCTACTGTC ACGGTCTGCCTGTCACTGACAGCAAAGAGGCTGGCCAGTAAAAACTGTGTGGTCAAAAATCTGGAAGCAG TGGAGACCCTGGGTTCCACGTCAGTCATCTGCTCAGACAAGACAGGAACTCTTACTCAGAACCGCATGAC GGTGTCTCATCTATGGTTTGACAACCATATCCACACGGCGGACACCACAGAAGACCAGTCAGGGCAAACG TTCGACCAATCGTCGGAGACCTGGCGGGCGCTGTGCCGCGTGCTCACCCTGTGCAACCGCGCTGCCTTCA AGTCTGGCCAGGACGCCGTGCCAGTGCCCAAGCGCATCGTGATCGGAGACGCATCTGAGACTGCGCTGCT CAAGTTCTCGGAGTTGACGTTGGGCAACGCCATGGGTTATCGGGACCGCTTCCCCAAAGTTTGCGAGATC CCCTTCAACTCCACCAACAAGTTTCAGCTGTCCATTCACACTCTGGAGGATCCGCGCGACCCCCGGCACT TGCTGGTGATGAAGGGCGCCCCAGAGCGCGTGCTGGAGCGTTGCAGCTCCATCCTCATCAAGGGCCAGGA GCTGCCCCTGGACGAGCAATGGCGTGAGGCCTTCCAGACAGCCTACCTTAGCCTGGGAGGCCTAGGCGAA CGCGTTCTTGGTTTCTGCCAGCTCTACCTGAATGAGAAGGACTACCCGCCTGGCTACACCTTTGATGTGG AGGCCATGAACTTTCCAAGTAGTGGCCTCTGCTTTGCGGGACTTGTATCCATGATTGACCCTCCCCGGGC CACCGTTCCAGATGCTGTGCTCAAATGCCGTACGGCAGGCATCCGGGTGATCATGGTGACTGGTGACCAT CCCATCACAGCCAAGGCCATTGCAGCCAGTGTGGGGATCATCTCGGAAGGCAGCGAGACAGTGGAAGACA TCGCCGCCCGCCTCCGAATGCCTGTAGACCAGGTTAATAAGAAGGATGCCCGGGCCTGTGTGATCAATGG CATGCAGTTGAAGGACATGGACCCATCTGAGCTGGTGGAGGCGCTGCGCACCCACCCTGAGATGGTGTTT GCTCGAACCAGTCCTCAGCAGAAGCTGGTGATTGTGGAGAGCTGTCAGCGACTGGGTGCCATTGTGGCTG TAACAGGGGATGGTGTGAATGACTCCCCAGCCCTGAAGAAGGCTGACATTGGTGTAGCCATGGGCATTGC TGGCTCCGATGCTGCTAAAAATGCTGCTGACATGATCTTGCTGGATGACAACTTCGCTTCCATTGTGACG GGCGTGGAGCAGGGCCGACTGATCTTTGACAACCTGAAGAAATCCATCGCCTATACACTGACCAAGAACA TTCCAGAACTGACACCCTACCTTATCTATATCACTGTCAGTGTGCCCCTACCCCTTGGGTGTATCACCAT TCTCTTCATAGAACTCTGCACAGATATCTTTCCATCTGTGTCCCTGGCATATGAAAAGGCCGAGAGTGAC ATCATGCACCTGCGCCCACGGAACCCCAGGCGGGACCGGTTGGTCAATGAACCCCTGGCTGCTTATTCCT ATTTTCAGATTGGTGCCATTCAGTCATTTGCCGGCTTTGCTGACTACTTCACGGCCATGGCCCAGGAGGG CTGGTTCCCTCTGCTGTGTGTGGGGCTGCGACCACAATGGGAGGACCACCATCTACAAGATCTTCAAGAC AGCTACGGCCAGGAATGGACATTTGGTCAGCGTCTGTACCAGCAGTACACCTGTTACACCGTGTTCTTCA TCAGCATCGAGATGTGCCAGATCGCTGATGTCCTCATCCGCAAGACACGCCGCCTCTCCGCTTTCCAGCA GGGATTCTTCAGGAACAGGATCCTGGTGATCGCCATCGTGTTTCAGGTCTGCATTGGCTGCTTCCTGTGC TACTGCCCAGGGATGCCCAACATCTTCAACTTCATGCCCATCCGGTTCCAGTGGTGGCTGGTCCCCATGC CCTTTGGCCTTCTCATCTTTGTCTATGATGAGATCCGGAAACTTGGAGTTCGCTGTTGCCCAGGGAGCTG GTGGGACCAGGAACTCTACTAT AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217427 representing NM_012509 Red=Cloning site Green=Tags(s) MGKAENYELYSVELGTGPGGNMAAKMSKKKAGGGGGKKKEKLENMKKEMEMNDHQLSVSELEQKYQTSAT KGLKASLAAELLLRDGPNALRPPRGTPEYVKFARQLAGGLQCLMWVAAAICLIAFAIQASEGDLTTDDNL YLALALIAVVVVTGCFGYYQEFKSTNIIASFKNLVPQQATVIRDGDKFQINADQLVVGDLVEMKGGDRVP ADIRILSAQGCKVDNSSLTGESEPQTRSPECTHESPLETRNIAFFSTMCLEGTAQGLVVSTGDRTIIGRI ASLASGVENEKTPIAIEIEHFVDIIAGLAILFGATFFVVAMCIGYTFLRAMVFFMAIVVAYVPEGLLATV TVCLSLTAKRLASKNCVVKNLEAVETLGSTSVICSDKTGTLTQNRMTVSHLWFDNHIHTADTTEDQSGQT FDQSSETWRALCRVLTLCNRAAFKSGQDAVPVPKRIVIGDASETALLKFSELTLGNAMGYRDRFPKVCEI PFNSTNKFQLSIHTLEDPRDPRHLLVMKGAPERVLERCSSILIKGQELPLDEQWREAFQTAYLSLGGLGE RVLGFCQLYLNEKDYPPGYTFDVEAMNFPSSGLCFAGLVSMIDPPRATVPDAVLKCRTAGIRVIMVTGDH PITAKAIAASVGIISEGSETVEDIAARLRMPVDQVNKKDARACVINGMQLKDMDPSELVEALRTHPEMVF ARTSPQQKLVIVESCQRLGAIVAVTGDGVNDSPALKKADIGVAMGIAGSDAAKNAADMILLDDNFASIVT GVEQGRLIFDNLKKSIAYTLTKNIPELTPYLIYITVSVPLPLGCITILFIELCTDIFPSVSLAYEKAESD IMHLRPRNPRRDRLVNEPLAAYSYFQIGAIQSFAGFADYFTAMAQEGWFPLLCVGLRPQWEDHHLQDLQD SYGQEWTFGQRLYQQYTCYTVFFISIEMCQIADVLIRKTRRLSAFQQGFFRNRILVIAIVFQVCIGCFLC YCPGMPNIFNFMPIRFQWWLVPMPFGLLIFVYDEIRKLGVRCCPGSWWDQELYY SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_012509
ORF Size	3102 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_012509.1, NP_036641.1
RefSeq Size	3105 bp
RefSeq ORF	3105 bp
Locus ID	24216
MW	114.1 kDa
Gene Summary	catalyzes H+ and K+ exchange; may play a role in secretion of stomach acids [RGD, Feb 2006]

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