Atp4a (NM_012509) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Hka; Hkatpc
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217427 representing NM_012509 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAAGGCAGAGAATTATGAATTGTACTCAGTGGAACTGGGGACTGGCCCTGGTGGGAACATGGCTG CCAAGATGAGCAAGAAGAAGGCGGGAGGCGGGGGAGGCAAGAAGAAGGAGAAGCTAGAGAACATGAAGAA GGAGATGGAGATGAACGACCACCAGTTGTCAGTGTCTGAGCTGGAGCAGAAGTACCAGACCAGTGCCACC AAGGGCCTGAAGGCGAGTCTGGCAGCTGAGCTGCTGCTGAGGGATGGGCCCAATGCACTCCGGCCACCTC GGGGCACCCCTGAGTACGTGAAGTTCGCCCGGCAGCTGGCAGGCGGTCTGCAGTGCCTCATGTGGGTGGC TGCAGCCATCTGCCTCATTGCCTTTGCGATTCAGGCCAGCGAGGGAGACCTGACCACTGATGACAATTTG TACCTGGCGTTGGCACTCATTGCTGTGGTTGTGGTCACTGGCTGTTTTGGCTACTACCAGGAGTTCAAGA GCACAAATATCATCGCCAGCTTCAAGAATCTTGTACCCCAGCAAGCCACAGTGATCCGAGACGGGGATAA GTTCCAGATCAACGCGGATCAGCTTGTGGTGGGCGACCTGGTAGAGATGAAAGGCGGGGACCGCGTCCCA GCAGACATCCGAATTCTGTCAGCCCAGGGCTGCAAGGTGGACAACTCCTCGCTTACTGGAGAGTCTGAAC CGCAGACCCGCTCACCTGAGTGTACACACGAGAGTCCCCTTGAGACCCGCAACATCGCCTTCTTCTCCAC CATGTGTCTGGAGGGCACGGCGCAGGGCTTGGTGGTGAGCACCGGTGATCGCACCATCATTGGACGCATC GCCTCTCTGGCTTCGGGTGTGGAAAACGAGAAGACTCCTATCGCGATCGAGATCGAACATTTTGTGGACA TCATTGCTGGCCTGGCCATCCTCTTCGGTGCCACATTCTTTGTGGTGGCCATGTGTATCGGCTATACCTT CCTTCGGGCCATGGTCTTCTTCATGGCCATTGTGGTAGCCTATGTGCCTGAGGGGCTGCTGGCTACTGTC ACGGTCTGCCTGTCACTGACAGCAAAGAGGCTGGCCAGTAAAAACTGTGTGGTCAAAAATCTGGAAGCAG TGGAGACCCTGGGTTCCACGTCAGTCATCTGCTCAGACAAGACAGGAACTCTTACTCAGAACCGCATGAC GGTGTCTCATCTATGGTTTGACAACCATATCCACACGGCGGACACCACAGAAGACCAGTCAGGGCAAACG TTCGACCAATCGTCGGAGACCTGGCGGGCGCTGTGCCGCGTGCTCACCCTGTGCAACCGCGCTGCCTTCA AGTCTGGCCAGGACGCCGTGCCAGTGCCCAAGCGCATCGTGATCGGAGACGCATCTGAGACTGCGCTGCT CAAGTTCTCGGAGTTGACGTTGGGCAACGCCATGGGTTATCGGGACCGCTTCCCCAAAGTTTGCGAGATC CCCTTCAACTCCACCAACAAGTTTCAGCTGTCCATTCACACTCTGGAGGATCCGCGCGACCCCCGGCACT TGCTGGTGATGAAGGGCGCCCCAGAGCGCGTGCTGGAGCGTTGCAGCTCCATCCTCATCAAGGGCCAGGA GCTGCCCCTGGACGAGCAATGGCGTGAGGCCTTCCAGACAGCCTACCTTAGCCTGGGAGGCCTAGGCGAA CGCGTTCTTGGTTTCTGCCAGCTCTACCTGAATGAGAAGGACTACCCGCCTGGCTACACCTTTGATGTGG AGGCCATGAACTTTCCAAGTAGTGGCCTCTGCTTTGCGGGACTTGTATCCATGATTGACCCTCCCCGGGC CACCGTTCCAGATGCTGTGCTCAAATGCCGTACGGCAGGCATCCGGGTGATCATGGTGACTGGTGACCAT CCCATCACAGCCAAGGCCATTGCAGCCAGTGTGGGGATCATCTCGGAAGGCAGCGAGACAGTGGAAGACA TCGCCGCCCGCCTCCGAATGCCTGTAGACCAGGTTAATAAGAAGGATGCCCGGGCCTGTGTGATCAATGG CATGCAGTTGAAGGACATGGACCCATCTGAGCTGGTGGAGGCGCTGCGCACCCACCCTGAGATGGTGTTT GCTCGAACCAGTCCTCAGCAGAAGCTGGTGATTGTGGAGAGCTGTCAGCGACTGGGTGCCATTGTGGCTG TAACAGGGGATGGTGTGAATGACTCCCCAGCCCTGAAGAAGGCTGACATTGGTGTAGCCATGGGCATTGC TGGCTCCGATGCTGCTAAAAATGCTGCTGACATGATCTTGCTGGATGACAACTTCGCTTCCATTGTGACG GGCGTGGAGCAGGGCCGACTGATCTTTGACAACCTGAAGAAATCCATCGCCTATACACTGACCAAGAACA TTCCAGAACTGACACCCTACCTTATCTATATCACTGTCAGTGTGCCCCTACCCCTTGGGTGTATCACCAT TCTCTTCATAGAACTCTGCACAGATATCTTTCCATCTGTGTCCCTGGCATATGAAAAGGCCGAGAGTGAC ATCATGCACCTGCGCCCACGGAACCCCAGGCGGGACCGGTTGGTCAATGAACCCCTGGCTGCTTATTCCT ATTTTCAGATTGGTGCCATTCAGTCATTTGCCGGCTTTGCTGACTACTTCACGGCCATGGCCCAGGAGGG CTGGTTCCCTCTGCTGTGTGTGGGGCTGCGACCACAATGGGAGGACCACCATCTACAAGATCTTCAAGAC AGCTACGGCCAGGAATGGACATTTGGTCAGCGTCTGTACCAGCAGTACACCTGTTACACCGTGTTCTTCA TCAGCATCGAGATGTGCCAGATCGCTGATGTCCTCATCCGCAAGACACGCCGCCTCTCCGCTTTCCAGCA GGGATTCTTCAGGAACAGGATCCTGGTGATCGCCATCGTGTTTCAGGTCTGCATTGGCTGCTTCCTGTGC TACTGCCCAGGGATGCCCAACATCTTCAACTTCATGCCCATCCGGTTCCAGTGGTGGCTGGTCCCCATGC CCTTTGGCCTTCTCATCTTTGTCTATGATGAGATCCGGAAACTTGGAGTTCGCTGTTGCCCAGGGAGCTG GTGGGACCAGGAACTCTACTATTAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_012509
Insert Size	3105 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_012509.1, NP_036641.1
RefSeq Size	3105 bp
RefSeq ORF	3105 bp
Locus ID	24216
Gene Summary	catalyzes H+ and K+ exchange; may play a role in secretion of stomach acids [RGD, Feb 2006]

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