Lrrk2 (NM_001191789) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217777 representing NM_001191789 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGTGGCGCCTGTCAGGGCTGCGACGAGGAAGAGGAGGAGGAGGCTCTGAAGAAGTTGATAGTCA GGCTGAATAATGTCCAGGAAGGCAAGCAGATCGAGACGTTGCTCCAGCTCCTGGAGGACATTCTGGTGTT CACCTACTCCGACCGCGCCTCCAAGTTATTTGAAGGCAAAAATGTCCACGTGCCTCTGTTGATAGTCCTG GACTCCTACATGAGAGTCGCCAGTGTGCAGCAGGTGGGGTGGTCACTTCTGTGCAAATTAATAGAAGTCT GTCCAGGGACATTGCAAAGCTTAATAGGACCCCAGGATATTGGGAATGATTGGGAAGTCCTTGGTATTCA CCGACTGATTCTTAAAATGTTAACTGTTCATCATGCCAACGTAAACCTGTCAATAGTTGGACTAAAAGCC TTAGATCTCCTCCTAGATTCAGGTAAAATTACTCTGCTGATACTGGATGAAGAATGTGATGTTTTCCTGT TAATTTTTGATGCCATGCACAGATATTCAGCCAACGAGGAAGTCCAGAAGCTTGCGTGCAAGGCTTTACA TGTGCTGTTCGAGAGAGTGTCCGAGGAGCAACTGACTGAGTTTGTGGAGAACAAAGATTACATGACCCTG CTGAGTACGTTCCGCAGCTTCAAGAGGGACGAGGAGATTGTGCACCATGTACTCTGCTGCCTGCATTCTC TGGCCGTCACTTGCAGCAATGTGGAGGTCCTCATGAGTGGGAATGTCAGGTGTTACAATATTGTGGTGGA AGCCATGAAAACATTCCCCACCAGTGAAAACATTCAAGAGGTGAGCTGCTCCTTGCTCCACAAGCTTACA TTAGGTAATTTTTTCAACATCCTGGTGTTGAACGAAGTCCATGTCTTTGTGGTGAAAGCCGTCCAGCGGT ATCCCGAGAACGTAGCCTTACAGATCTCTGCACTCAGCTGCTTAGCCCTCCTCACCGAGACTATTTTCTT AAACCAAGACCTGGAAGAAAGAAGTGAGACTCAGGAAAACAGCGATGAGGACAGTGAGAAGCCTTTCTGG TTGGAACCCTGCTATAAAGCCCTGATGCGCCATCGAAAGAACAAACACGTGCAGGAGGCCGCCTGCTGGG CCCTAAATAATCTCCTCATGTACCAGAGCAGTTTGCACGAGAAGATTGGAGATGAAGATGGCCAGTTCCC GGCGCACAGGGAAGTGATGCTGTCTATGCTGATGCACTCTTCTTCCAAAGACGTCTTCCAAGCAGCTGCG CATGCTCTGTCCACTCTCTTGGAACAAAACGTTAATTTCAGGAAAATCCTGCTTGCAAAAGGAGTGTACC TGAATGTCTTGGAGTTGATGCAGCGGCACGCCCAGGTTCCTGAGGTGGCAGAGAGTGGCTGCAAGATGCT GAGTCATCTGTTTGAAGGAAGCAACCCTTCTTTGGATACAGTGGCGGCAGTGATCCCCAAAATACTAACA GTGATGAGAACCCATGGAACGTCTCTGTCAGTCCAGCTGGAGGCACTGCGAGCTCTTCTGCATTTTGTGG TGCCGGGAGTATCAGAAGATTCCAGGGATGACTCGCGATGCCAACCAAACGTGCTCAGAACACAGTGCTT CAGGACTGACATCCACAAGCTGGTTCTAGCCGCTCTGAACAGGTTCATTGGGAATCCCGGGATTCAGAAA TGTGGATTGAAAGTCATCTCTTCTTTCGCACATCTTCCCGATGCCTTAGAGATGTTATCCCTGCATGGAG CAGTTGACTCAGTCCTCCATACCTTACAGATGTATCCAGATGACCAAGAAATTCAGTGTCTGGGCTTACA CCTTATGGGATGCCTGATGACAAAGAAGAATTTCTGCATAGGGACAGGGCACCTCCTGGCAAAAATTCTG GCTTCCACCTTGCAGCGATTTAAAGATGTTGCTGAAGTACAGACTACAGGATTACAGACGGTCTTGTCAA TGCTTGACCTGTCCGTATCTTTCTCCAAGCTGCTAGTGCACTATTCATTTGATGTGGTGATGTTTCATCA GATGTCTTCCGGTGTCCTGGAACAAAAGGATGAGCAGTTTCTCAACTTATGCTGCAAATGCTTTGCAAAA GTGGCTGTGGATGATGAGCTGAAAAGCAAGATGCTAGAGAGAGCCTGCGATCAGAACAACAGCATCATGG TCGAATGTTTGCTCCTCTTGGGAGCCGATGCCAATCAAGCGAAGGGGGCAACTTCTTTAATCTATCAGGT ATGTGAGAAAGAGAGCAGCCCTAAATTGGTGGAACTATTGCTTAACAGTGGGTGCCGTGAACAAGATGTA CGGAAAGCCCTGACAGTAAGCATCCAAAAGGGCGACAACCAGGTCATCAGCTTACTCCTGAGGAGACTTG CCCTGGACCTGGCCAACAACAGCATTTGCCTTGGAGGATTTTGCATAGGAAAACTTGATCCTTCTTGGCT AGGCCCTTTATTTCCAGATAAGTCATCTAATTTGAGGAAACAAACAAATGCGGGGTCTGTCCTAGCGAGG AAAGTGCTCCGGTATCAGATGAGAAACACTCTTCAAGAAGGCGTGGCCTCAGGCAGTGAGGGCAACTTCT CTGAGGATGCGCTGGCGAAATTTGGCGAATGGACCTTCATTCCCGACTCTTCTATGGACAGTGTGTTTGG CCAGAGTGACGATCTGGATAGCGAAGGCAGCGAGAGCTCCTTTCTGGTGAAGAAGAAGTCCAACTCAGTT AGTGTAGGAGAAGTTTACAGGGACCTAGCTCTGCAGCGCTGCTCACCAAATGCTCAGAGGCACTCCAGTT CCTTGGGTCCTGTTTTTGATCACGAAGATCTACTGAGACGAAAAAGAAAAATACTGTCCTCAGATGAGTC TCTCAGATCCTCAAGGCTGCAGTCCCATACGAGACAATCAGATAGCTCTTCTTCTCTGGCTTCTGAGAGA GAACACATCACGTCTTTAGACCTTTCTGCCAACGAACTGAAAGATATTGATGCTCTGGGCCAGAAGTGTT GCCTCAGTAGCCACCTGGAGCATCTCACCAAGCTGGAACTTCACCAGAATTCACTCACGAGCTTCCCACA ACAGCTGTGTGAGACTCTGAAGTGCTTGACACATCTGGATTTGCACAGTAACAAATTCGCCACCTTTCCC TCCTTCATGTTGAAAATGCCAAGTGTTATCCACCTAGACGCCTCTCGAAATGACATCGGACCAACAGTTG TTTTAGACCCTGTGGTGAAGTGTCCAAGCCTCAAACAGTTTAACCTGTCCTACAACCAGCTCTCTTCCAT CCCAGAGAACCTGGACCAAGTGGTGGAGAAACTGGAGCAGCTCCTACTGGAAGGAAACAAAATATCCGGG ATTTGTTCTCCCTTGAGCCTGAAGGAACTGAAGATTTTAAACCTTAGTAAAAACCACATTCCATCCCTAC CTGAAGACTTTCTCGAGGCTTGCCCGAAAGTGGAGAGCTTCAGTGCCCGCATGAATTTTCTCGCTGCAAT GCCTGCCTTACCGTCTTCCATAACTAGCTTAAAATTGTCTCAAAACTCTTTCACGTGCATTCCAGAAGCG ATCTTCAGTCTTCCACACTTGCGGTCCTTGGATATGAGTCACAACAACATTGAACACCTGCCGGGACCTG CACATTGGAAGTCTCTGAACTTAAGGGAACTCATTTTTAGCAAGAATCAGATCAGCACCTTAGACTTGAG CGAAAACCCACACATATGGTCAAGAGTAGAGAAGCTGCATCTCTCTCATAATAAACTGAAAGAGATTCCT CCAGAAATTGGCCGTCTTGAAAACCTGACATCTCTTGATGTCAGTTACAACCTGGAACTGAGGTCCTTTC CAAACGAAATGGGGAAGTTAAGCAAAATATGGGATCTTCCCTTGGATGGACTGCACCTCAACTTTGACTT TAAGCACATAGGATGCAAAGCCAAAGACATCATAAGGTTTCTACAACAACGTCTGAAAAAGGCCGTGCCC TACAACCGAATGAAGCTCATGATTGTGGGCAATACGGGGAGTGGTAAGACCACTCTACTGCAGCAGCTCA TGAAAATGAAGAAATCAGAACTCGGCATGCAGGGCGCCACGGTTGGCATAGACGTGCGAGACTGGCCCAT CCAAATACGAGGCAAAAGGAAAAAGGACCTTGTTCTAAACGTGTGGGACTTTGCAGGCCGTGAGGAATTC TACAGCACTCACCCCCACTTCATGACCCAGAGAGCCCTGTACCTGGCTGTCTACGACCTCAGCAAGGGGC AGGCGGAGGTGGATGCCATGAAGCCCTGGCTCTTCAACATCAAGGCTCGTGCCTCTTCTTCCCCGGTGAT TCTGGTGGGCACACATTTGGATGTTTCTGATGAGAAGCAGCGCAAAGCCTGCATAGGCAAAATCACGAAG GAACTCCTTAATAAGCGAGGATTCCCCACCATCCGGGACTACCACTTCGTGAATGCCACTGAGGAGTCGG ATGCGCTGGCAAAGCTCCGGAAAACCATCATAAATGAGAGTCTTAATTTCAAGATCCGAGATCAGCCCGT GGTTGGGCAGCTAATTCCAGATTGCTACGTAGAACTGGAGAAAATAATCTTATCGGAGCGTAAAGCTGTA CCAACGGAGTTTCCTGTAATTAACCGGAAACACTTACTCCAGCTGGTGAAGGAACACCAGCTGCAGCTGG ATGAGAACGAGCTCCCCCACGCTGTTCACTTCCTGAATGAGTCAGGAGTTCTTCTGCATTTTCAAGACCC CGCATTGCAGCTGAGTGACCTGTACTTTGTGGAACCCAAGTGGCTTTGTAAAGTCATGGCACAGATTTTG ACCGTGAAAGTGGACGGCTGCCTGAAGCATCCTAAGGGCATCATTTCACGGAGAGATGTGGAAAAATTCC TTTCCAAGAAAAAGCGATTCCCTAAGAACTACATGGCGCAGTACTTCAAACTTTTAGAAAAATTTCAGAT CGCATTACCAATAGGGGAAGAATATCTGCTGGTTCCAAGCAGCTTATCTGACCACAGGCCAGTGATAGAG CTCCCCCACTGTGAGAACTCTGAGATCATCATCCGGCTGTATGAAATGCCATACTTTCCAATGGGATTTT GGTCAAGATTGATTAACCGATTACTTGAAATCTCACCTTTCATGCTTTCTGGAAGAGAGAGAGCACTACG CCCAAACCGAATGTACTGGCGCCAAGGCATCTACTTGAATTGGTCTCCAGAAGCCTACTGTCTGGTGGGC TCTGAAGTCTTAGACAGTCGCCCAGAGAGTTTCTTGAAAATCACAGTTCCATCTTGTAGAAAAGGTTGTA TTCTTTTGGGCCGAGTTGTGGATCATATTGACTCACTCATGGAAGAATGGTTTCCTGGATTGCTGGAGAT TGACATTTGTGGGGAAGGAGAAACTTTGTTGAAAAAATGGGCATTGTATAGTTTTAATGATGGCGAAGAA CATCAGAAGATCTTGCTTGATGAGTTGATGAAGAAGGCTGAAGAAGGAGACCTGTTAATAAATCCAGATC AACCAAGGCTCACCATTCCAATATCCCAGATTGCTCCGGACTTGATCTTGGCTGACCTGCCTAGAAATAT TATGTTGAACAATGACGAACTGGAATTTGAGGAAGCACCCGAGTTTCTCTTAGGTGATGGAAGTTTCGGA TCAGTTTATCGAGCTGCCTACGAAGGAGAGGAAGTGGCTGTGAAGATTTTTAATAAGCACACATCGCTTA GGCTGTTAAGACAAGAGTTGGTGGTACTCTGTCATCTCCACCATCCCAGCTTGATCTCCCTGTTGGCGGC TGGGATTCGTCCTCGGATGCTGGTAATGGAGTTGGCCTCCAAGGGTTCCTTGGATCGCCTGCTGCAGCAG GACAAAGCCAGCCTCACCCGGACCCTCCAGCACAGAATCGCATTGCATGTGGCCGATGGCCTGAGATATC TGCACTCGGCCATGATTATTTACCGTGATCTGAAGCCCCACAACGTGCTACTCTTCACCCTGTATCCCAA TGCCGCCATCATTGCGAAGATTGCGGACTACGGGATTGCACAGTACTGCTGTAGGATGGGAATAAAGACC TCAGAGGGCACCCCAGGGTTCCGAGCACCTGAAGTTGCCAGAGGAAATGTCATTTATAACCAACAGGCTG ATGTTTATTCTTTTGGCTTACTACTTCATGATATCTGGACAACTGGGAATAGAATCATGGAGGGTTTGAG GTTTCCAAATGAGTTTGATGAACTGGCCATACAAGGGAAATTGCCAGACCCAGTTAAAGAATATGGCTGT GCCCCGTGGCCTATGGTTGAGAAGTTAATTACAAAATGTTTGAAAGAAAATCCTCAAGAAAGACCCACTT CTGCCCAGGTCTTTGACATTTTGAATTCAGCTGAGTTAATTTGCCTCATGCGACACATTTTCATACCTAA GGACATCACTGTTGAATGCATAGCTGCTACAAACCTCAATAGCAAGCGAGCGACTCTCTGGTTGGGCTGT GGGAACACAGAAAAAGGGCAGCTTTCCTTACTTGACTTGAACACGGAAAGATACAGCTATGAGGAAGTTA CTGATAGTAGAATACTGTGCCTGGCTTTGGTGCATCTTGCTGCTGAGAAAGAGAGCTGGGTTGTGTGTGG GACACAGTCCGGAGCTCTCCTGGTCATCAATGCTGAAGATGAGACAAGGAGACACACCCTCGACAAGATG ACTGATTCTGTTACTTGCTTGTATTGCAATTCCTTTGCCAAGCAGAGCAAGCAAAGTCACTTCCTTTTGG TGGGAACTGCTGATGGCAACTTAATGATATTTGAAGATAAGACCATTAAGTGTAAAGGAGCTGCCCCATT GAAGACACTACACATAGGCGATGTCAGTACGCCCCTGATGTGCCTGAGCGAGTCCATGAATTCATCTGAA AGACACATCACATGGGGAGGGTGTGGCACAAAGATCTTCTCCTTTTCCAATGATTTCACCATTCAGAAAC TCATCGAGACAAGAACCAACCAGCTGTTTTCTTACTCAGCGTTCAGCGATTCTAACATCATAGCGGTGGC AGTGGACACAGCGCTTTATATTGCCAAGAAAAACAGCCCTGTCGTAGAGGTGTGGGACAAGAAGACAGAA AAACTCTGTGAACTAATAGACTGTGTGCACTTCTTAAAGGAGGTGATGGTGAAAATAAACAAGGACTCGA AGCACAAGCTGTCCTACTCTGGGAGGGTGAAGGCACTCTGCCTGCAGAAGAACACAGCTCTCTGGATCGG AACTGGAGGAGGCCACATCTTACTCCTGGATCTTTCTACACGGCGAGTCATCCGCACCATCCACAATTTC TGTGATTCCGTGAGAGCCATGGCCACAGCTCAGTTAGGCAACCTTAAAAATGTCATGCTGGTTTTGGGGT ACAAGCGGAAGAGTACAGAAGGAACCCAAGAACAAAAAGAGATACAATCTTGTTTGTCTATTTGGGACCT CAATCTTCCACATGAAGTGCAAAACTTAGAAAAACACATTGAAGTAAGAACAGAACTGGCTGATAAAATG AGGAAAACATCTGTCGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217777 representing NM_001191789 Red=Cloning site Green=Tags(s) MASGACQGCDEEEEEEALKKLIVRLNNVQEGKQIETLLQLLEDILVFTYSDRASKLFEGKNVHVPLLIVL DSYMRVASVQQVGWSLLCKLIEVCPGTLQSLIGPQDIGNDWEVLGIHRLILKMLTVHHANVNLSIVGLKA LDLLLDSGKITLLILDEECDVFLLIFDAMHRYSANEEVQKLACKALHVLFERVSEEQLTEFVENKDYMTL LSTFRSFKRDEEIVHHVLCCLHSLAVTCSNVEVLMSGNVRCYNIVVEAMKTFPTSENIQEVSCSLLHKLT LGNFFNILVLNEVHVFVVKAVQRYPENVALQISALSCLALLTETIFLNQDLEERSETQENSDEDSEKPFW LEPCYKALMRHRKNKHVQEAACWALNNLLMYQSSLHEKIGDEDGQFPAHREVMLSMLMHSSSKDVFQAAA HALSTLLEQNVNFRKILLAKGVYLNVLELMQRHAQVPEVAESGCKMLSHLFEGSNPSLDTVAAVIPKILT VMRTHGTSLSVQLEALRALLHFVVPGVSEDSRDDSRCQPNVLRTQCFRTDIHKLVLAALNRFIGNPGIQK CGLKVISSFAHLPDALEMLSLHGAVDSVLHTLQMYPDDQEIQCLGLHLMGCLMTKKNFCIGTGHLLAKIL ASTLQRFKDVAEVQTTGLQTVLSMLDLSVSFSKLLVHYSFDVVMFHQMSSGVLEQKDEQFLNLCCKCFAK VAVDDELKSKMLERACDQNNSIMVECLLLLGADANQAKGATSLIYQVCEKESSPKLVELLLNSGCREQDV RKALTVSIQKGDNQVISLLLRRLALDLANNSICLGGFCIGKLDPSWLGPLFPDKSSNLRKQTNAGSVLAR KVLRYQMRNTLQEGVASGSEGNFSEDALAKFGEWTFIPDSSMDSVFGQSDDLDSEGSESSFLVKKKSNSV SVGEVYRDLALQRCSPNAQRHSSSLGPVFDHEDLLRRKRKILSSDESLRSSRLQSHTRQSDSSSSLASER EHITSLDLSANELKDIDALGQKCCLSSHLEHLTKLELHQNSLTSFPQQLCETLKCLTHLDLHSNKFATFP SFMLKMPSVIHLDASRNDIGPTVVLDPVVKCPSLKQFNLSYNQLSSIPENLDQVVEKLEQLLLEGNKISG ICSPLSLKELKILNLSKNHIPSLPEDFLEACPKVESFSARMNFLAAMPALPSSITSLKLSQNSFTCIPEA IFSLPHLRSLDMSHNNIEHLPGPAHWKSLNLRELIFSKNQISTLDLSENPHIWSRVEKLHLSHNKLKEIP PEIGRLENLTSLDVSYNLELRSFPNEMGKLSKIWDLPLDGLHLNFDFKHIGCKAKDIIRFLQQRLKKAVP YNRMKLMIVGNTGSGKTTLLQQLMKMKKSELGMQGATVGIDVRDWPIQIRGKRKKDLVLNVWDFAGREEF YSTHPHFMTQRALYLAVYDLSKGQAEVDAMKPWLFNIKARASSSPVILVGTHLDVSDEKQRKACIGKITK ELLNKRGFPTIRDYHFVNATEESDALAKLRKTIINESLNFKIRDQPVVGQLIPDCYVELEKIILSERKAV PTEFPVINRKHLLQLVKEHQLQLDENELPHAVHFLNESGVLLHFQDPALQLSDLYFVEPKWLCKVMAQIL TVKVDGCLKHPKGIISRRDVEKFLSKKKRFPKNYMAQYFKLLEKFQIALPIGEEYLLVPSSLSDHRPVIE LPHCENSEIIIRLYEMPYFPMGFWSRLINRLLEISPFMLSGRERALRPNRMYWRQGIYLNWSPEAYCLVG SEVLDSRPESFLKITVPSCRKGCILLGRVVDHIDSLMEEWFPGLLEIDICGEGETLLKKWALYSFNDGEE HQKILLDELMKKAEEGDLLINPDQPRLTIPISQIAPDLILADLPRNIMLNNDELEFEEAPEFLLGDGSFG SVYRAAYEGEEVAVKIFNKHTSLRLLRQELVVLCHLHHPSLISLLAAGIRPRMLVMELASKGSLDRLLQQ DKASLTRTLQHRIALHVADGLRYLHSAMIIYRDLKPHNVLLFTLYPNAAIIAKIADYGIAQYCCRMGIKT SEGTPGFRAPEVARGNVIYNQQADVYSFGLLLHDIWTTGNRIMEGLRFPNEFDELAIQGKLPDPVKEYGC APWPMVEKLITKCLKENPQERPTSAQVFDILNSAELICLMRHIFIPKDITVECIAATNLNSKRATLWLGC GNTEKGQLSLLDLNTERYSYEEVTDSRILCLALVHLAAEKESWVVCGTQSGALLVINAEDETRRHTLDKM TDSVTCLYCNSFAKQSKQSHFLLVGTADGNLMIFEDKTIKCKGAAPLKTLHIGDVSTPLMCLSESMNSSE RHITWGGCGTKIFSFSNDFTIQKLIETRTNQLFSYSAFSDSNIIAVAVDTALYIAKKNSPVVEVWDKKTE KLCELIDCVHFLKEVMVKINKDSKHKLSYSGRVKALCLQKNTALWIGTGGGHILLLDLSTRRVIRTIHNF CDSVRAMATAQLGNLKNVMLVLGYKRKSTEGTQEQKEIQSCLSIWDLNLPHEVQNLEKHIEVRTELADKM RKTSVE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191789
ORF Size	7578 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191789.1, NP_001178718.1
RefSeq Size	7581 bp
RefSeq ORF	7581 bp
Locus ID	300160
MW	284.9 kDa

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