Lrrk2 (NM_001191789) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217777 representing NM_001191789 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGTGGCGCCTGTCAGGGCTGCGACGAGGAAGAGGAGGAGGAGGCTCTGAAGAAGTTGATAGTCA GGCTGAATAATGTCCAGGAAGGCAAGCAGATCGAGACGTTGCTCCAGCTCCTGGAGGACATTCTGGTGTT CACCTACTCCGACCGCGCCTCCAAGTTATTTGAAGGCAAAAATGTCCACGTGCCTCTGTTGATAGTCCTG GACTCCTACATGAGAGTCGCCAGTGTGCAGCAGGTGGGGTGGTCACTTCTGTGCAAATTAATAGAAGTCT GTCCAGGGACATTGCAAAGCTTAATAGGACCCCAGGATATTGGGAATGATTGGGAAGTCCTTGGTATTCA CCGACTGATTCTTAAAATGTTAACTGTTCATCATGCCAACGTAAACCTGTCAATAGTTGGACTAAAAGCC TTAGATCTCCTCCTAGATTCAGGTAAAATTACTCTGCTGATACTGGATGAAGAATGTGATGTTTTCCTGT TAATTTTTGATGCCATGCACAGATATTCAGCCAACGAGGAAGTCCAGAAGCTTGCGTGCAAGGCTTTACA TGTGCTGTTCGAGAGAGTGTCCGAGGAGCAACTGACTGAGTTTGTGGAGAACAAAGATTACATGACCCTG CTGAGTACGTTCCGCAGCTTCAAGAGGGACGAGGAGATTGTGCACCATGTACTCTGCTGCCTGCATTCTC TGGCCGTCACTTGCAGCAATGTGGAGGTCCTCATGAGTGGGAATGTCAGGTGTTACAATATTGTGGTGGA AGCCATGAAAACATTCCCCACCAGTGAAAACATTCAAGAGGTGAGCTGCTCCTTGCTCCACAAGCTTACA TTAGGTAATTTTTTCAACATCCTGGTGTTGAACGAAGTCCATGTCTTTGTGGTGAAAGCCGTCCAGCGGT ATCCCGAGAACGTAGCCTTACAGATCTCTGCACTCAGCTGCTTAGCCCTCCTCACCGAGACTATTTTCTT AAACCAAGACCTGGAAGAAAGAAGTGAGACTCAGGAAAACAGCGATGAGGACAGTGAGAAGCCTTTCTGG TTGGAACCCTGCTATAAAGCCCTGATGCGCCATCGAAAGAACAAACACGTGCAGGAGGCCGCCTGCTGGG CCCTAAATAATCTCCTCATGTACCAGAGCAGTTTGCACGAGAAGATTGGAGATGAAGATGGCCAGTTCCC GGCGCACAGGGAAGTGATGCTGTCTATGCTGATGCACTCTTCTTCCAAAGACGTCTTCCAAGCAGCTGCG CATGCTCTGTCCACTCTCTTGGAACAAAACGTTAATTTCAGGAAAATCCTGCTTGCAAAAGGAGTGTACC TGAATGTCTTGGAGTTGATGCAGCGGCACGCCCAGGTTCCTGAGGTGGCAGAGAGTGGCTGCAAGATGCT GAGTCATCTGTTTGAAGGAAGCAACCCTTCTTTGGATACAGTGGCGGCAGTGATCCCCAAAATACTAACA GTGATGAGAACCCATGGAACGTCTCTGTCAGTCCAGCTGGAGGCACTGCGAGCTCTTCTGCATTTTGTGG TGCCGGGAGTATCAGAAGATTCCAGGGATGACTCGCGATGCCAACCAAACGTGCTCAGAACACAGTGCTT CAGGACTGACATCCACAAGCTGGTTCTAGCCGCTCTGAACAGGTTCATTGGGAATCCCGGGATTCAGAAA TGTGGATTGAAAGTCATCTCTTCTTTCGCACATCTTCCCGATGCCTTAGAGATGTTATCCCTGCATGGAG CAGTTGACTCAGTCCTCCATACCTTACAGATGTATCCAGATGACCAAGAAATTCAGTGTCTGGGCTTACA CCTTATGGGATGCCTGATGACAAAGAAGAATTTCTGCATAGGGACAGGGCACCTCCTGGCAAAAATTCTG GCTTCCACCTTGCAGCGATTTAAAGATGTTGCTGAAGTACAGACTACAGGATTACAGACGGTCTTGTCAA TGCTTGACCTGTCCGTATCTTTCTCCAAGCTGCTAGTGCACTATTCATTTGATGTGGTGATGTTTCATCA GATGTCTTCCGGTGTCCTGGAACAAAAGGATGAGCAGTTTCTCAACTTATGCTGCAAATGCTTTGCAAAA GTGGCTGTGGATGATGAGCTGAAAAGCAAGATGCTAGAGAGAGCCTGCGATCAGAACAACAGCATCATGG TCGAATGTTTGCTCCTCTTGGGAGCCGATGCCAATCAAGCGAAGGGGGCAACTTCTTTAATCTATCAGGT ATGTGAGAAAGAGAGCAGCCCTAAATTGGTGGAACTATTGCTTAACAGTGGGTGCCGTGAACAAGATGTA CGGAAAGCCCTGACAGTAAGCATCCAAAAGGGCGACAACCAGGTCATCAGCTTACTCCTGAGGAGACTTG CCCTGGACCTGGCCAACAACAGCATTTGCCTTGGAGGATTTTGCATAGGAAAACTTGATCCTTCTTGGCT AGGCCCTTTATTTCCAGATAAGTCATCTAATTTGAGGAAACAAACAAATGCGGGGTCTGTCCTAGCGAGG AAAGTGCTCCGGTATCAGATGAGAAACACTCTTCAAGAAGGCGTGGCCTCAGGCAGTGAGGGCAACTTCT CTGAGGATGCGCTGGCGAAATTTGGCGAATGGACCTTCATTCCCGACTCTTCTATGGACAGTGTGTTTGG CCAGAGTGACGATCTGGATAGCGAAGGCAGCGAGAGCTCCTTTCTGGTGAAGAAGAAGTCCAACTCAGTT AGTGTAGGAGAAGTTTACAGGGACCTAGCTCTGCAGCGCTGCTCACCAAATGCTCAGAGGCACTCCAGTT CCTTGGGTCCTGTTTTTGATCACGAAGATCTACTGAGACGAAAAAGAAAAATACTGTCCTCAGATGAGTC TCTCAGATCCTCAAGGCTGCAGTCCCATACGAGACAATCAGATAGCTCTTCTTCTCTGGCTTCTGAGAGA GAACACATCACGTCTTTAGACCTTTCTGCCAACGAACTGAAAGATATTGATGCTCTGGGCCAGAAGTGTT GCCTCAGTAGCCACCTGGAGCATCTCACCAAGCTGGAACTTCACCAGAATTCACTCACGAGCTTCCCACA ACAGCTGTGTGAGACTCTGAAGTGCTTGACACATCTGGATTTGCACAGTAACAAATTCGCCACCTTTCCC TCCTTCATGTTGAAAATGCCAAGTGTTATCCACCTAGACGCCTCTCGAAATGACATCGGACCAACAGTTG TTTTAGACCCTGTGGTGAAGTGTCCAAGCCTCAAACAGTTTAACCTGTCCTACAACCAGCTCTCTTCCAT CCCAGAGAACCTGGACCAAGTGGTGGAGAAACTGGAGCAGCTCCTACTGGAAGGAAACAAAATATCCGGG ATTTGTTCTCCCTTGAGCCTGAAGGAACTGAAGATTTTAAACCTTAGTAAAAACCACATTCCATCCCTAC CTGAAGACTTTCTCGAGGCTTGCCCGAAAGTGGAGAGCTTCAGTGCCCGCATGAATTTTCTCGCTGCAAT GCCTGCCTTACCGTCTTCCATAACTAGCTTAAAATTGTCTCAAAACTCTTTCACGTGCATTCCAGAAGCG ATCTTCAGTCTTCCACACTTGCGGTCCTTGGATATGAGTCACAACAACATTGAACACCTGCCGGGACCTG CACATTGGAAGTCTCTGAACTTAAGGGAACTCATTTTTAGCAAGAATCAGATCAGCACCTTAGACTTGAG CGAAAACCCACACATATGGTCAAGAGTAGAGAAGCTGCATCTCTCTCATAATAAACTGAAAGAGATTCCT CCAGAAATTGGCCGTCTTGAAAACCTGACATCTCTTGATGTCAGTTACAACCTGGAACTGAGGTCCTTTC CAAACGAAATGGGGAAGTTAAGCAAAATATGGGATCTTCCCTTGGATGGACTGCACCTCAACTTTGACTT TAAGCACATAGGATGCAAAGCCAAAGACATCATAAGGTTTCTACAACAACGTCTGAAAAAGGCCGTGCCC TACAACCGAATGAAGCTCATGATTGTGGGCAATACGGGGAGTGGTAAGACCACTCTACTGCAGCAGCTCA TGAAAATGAAGAAATCAGAACTCGGCATGCAGGGCGCCACGGTTGGCATAGACGTGCGAGACTGGCCCAT CCAAATACGAGGCAAAAGGAAAAAGGACCTTGTTCTAAACGTGTGGGACTTTGCAGGCCGTGAGGAATTC TACAGCACTCACCCCCACTTCATGACCCAGAGAGCCCTGTACCTGGCTGTCTACGACCTCAGCAAGGGGC AGGCGGAGGTGGATGCCATGAAGCCCTGGCTCTTCAACATCAAGGCTCGTGCCTCTTCTTCCCCGGTGAT TCTGGTGGGCACACATTTGGATGTTTCTGATGAGAAGCAGCGCAAAGCCTGCATAGGCAAAATCACGAAG GAACTCCTTAATAAGCGAGGATTCCCCACCATCCGGGACTACCACTTCGTGAATGCCACTGAGGAGTCGG ATGCGCTGGCAAAGCTCCGGAAAACCATCATAAATGAGAGTCTTAATTTCAAGATCCGAGATCAGCCCGT GGTTGGGCAGCTAATTCCAGATTGCTACGTAGAACTGGAGAAAATAATCTTATCGGAGCGTAAAGCTGTA CCAACGGAGTTTCCTGTAATTAACCGGAAACACTTACTCCAGCTGGTGAAGGAACACCAGCTGCAGCTGG ATGAGAACGAGCTCCCCCACGCTGTTCACTTCCTGAATGAGTCAGGAGTTCTTCTGCATTTTCAAGACCC CGCATTGCAGCTGAGTGACCTGTACTTTGTGGAACCCAAGTGGCTTTGTAAAGTCATGGCACAGATTTTG ACCGTGAAAGTGGACGGCTGCCTGAAGCATCCTAAGGGCATCATTTCACGGAGAGATGTGGAAAAATTCC TTTCCAAGAAAAAGCGATTCCCTAAGAACTACATGGCGCAGTACTTCAAACTTTTAGAAAAATTTCAGAT CGCATTACCAATAGGGGAAGAATATCTGCTGGTTCCAAGCAGCTTATCTGACCACAGGCCAGTGATAGAG CTCCCCCACTGTGAGAACTCTGAGATCATCATCCGGCTGTATGAAATGCCATACTTTCCAATGGGATTTT GGTCAAGATTGATTAACCGATTACTTGAAATCTCACCTTTCATGCTTTCTGGAAGAGAGAGAGCACTACG CCCAAACCGAATGTACTGGCGCCAAGGCATCTACTTGAATTGGTCTCCAGAAGCCTACTGTCTGGTGGGC TCTGAAGTCTTAGACAGTCGCCCAGAGAGTTTCTTGAAAATCACAGTTCCATCTTGTAGAAAAGGTTGTA TTCTTTTGGGCCGAGTTGTGGATCATATTGACTCACTCATGGAAGAATGGTTTCCTGGATTGCTGGAGAT TGACATTTGTGGGGAAGGAGAAACTTTGTTGAAAAAATGGGCATTGTATAGTTTTAATGATGGCGAAGAA CATCAGAAGATCTTGCTTGATGAGTTGATGAAGAAGGCTGAAGAAGGAGACCTGTTAATAAATCCAGATC AACCAAGGCTCACCATTCCAATATCCCAGATTGCTCCGGACTTGATCTTGGCTGACCTGCCTAGAAATAT TATGTTGAACAATGACGAACTGGAATTTGAGGAAGCACCCGAGTTTCTCTTAGGTGATGGAAGTTTCGGA TCAGTTTATCGAGCTGCCTACGAAGGAGAGGAAGTGGCTGTGAAGATTTTTAATAAGCACACATCGCTTA GGCTGTTAAGACAAGAGTTGGTGGTACTCTGTCATCTCCACCATCCCAGCTTGATCTCCCTGTTGGCGGC TGGGATTCGTCCTCGGATGCTGGTAATGGAGTTGGCCTCCAAGGGTTCCTTGGATCGCCTGCTGCAGCAG GACAAAGCCAGCCTCACCCGGACCCTCCAGCACAGAATCGCATTGCATGTGGCCGATGGCCTGAGATATC TGCACTCGGCCATGATTATTTACCGTGATCTGAAGCCCCACAACGTGCTACTCTTCACCCTGTATCCCAA TGCCGCCATCATTGCGAAGATTGCGGACTACGGGATTGCACAGTACTGCTGTAGGATGGGAATAAAGACC TCAGAGGGCACCCCAGGGTTCCGAGCACCTGAAGTTGCCAGAGGAAATGTCATTTATAACCAACAGGCTG ATGTTTATTCTTTTGGCTTACTACTTCATGATATCTGGACAACTGGGAATAGAATCATGGAGGGTTTGAG GTTTCCAAATGAGTTTGATGAACTGGCCATACAAGGGAAATTGCCAGACCCAGTTAAAGAATATGGCTGT GCCCCGTGGCCTATGGTTGAGAAGTTAATTACAAAATGTTTGAAAGAAAATCCTCAAGAAAGACCCACTT CTGCCCAGGTCTTTGACATTTTGAATTCAGCTGAGTTAATTTGCCTCATGCGACACATTTTCATACCTAA GGACATCACTGTTGAATGCATAGCTGCTACAAACCTCAATAGCAAGCGAGCGACTCTCTGGTTGGGCTGT GGGAACACAGAAAAAGGGCAGCTTTCCTTACTTGACTTGAACACGGAAAGATACAGCTATGAGGAAGTTA CTGATAGTAGAATACTGTGCCTGGCTTTGGTGCATCTTGCTGCTGAGAAAGAGAGCTGGGTTGTGTGTGG GACACAGTCCGGAGCTCTCCTGGTCATCAATGCTGAAGATGAGACAAGGAGACACACCCTCGACAAGATG ACTGATTCTGTTACTTGCTTGTATTGCAATTCCTTTGCCAAGCAGAGCAAGCAAAGTCACTTCCTTTTGG TGGGAACTGCTGATGGCAACTTAATGATATTTGAAGATAAGACCATTAAGTGTAAAGGAGCTGCCCCATT GAAGACACTACACATAGGCGATGTCAGTACGCCCCTGATGTGCCTGAGCGAGTCCATGAATTCATCTGAA AGACACATCACATGGGGAGGGTGTGGCACAAAGATCTTCTCCTTTTCCAATGATTTCACCATTCAGAAAC TCATCGAGACAAGAACCAACCAGCTGTTTTCTTACTCAGCGTTCAGCGATTCTAACATCATAGCGGTGGC AGTGGACACAGCGCTTTATATTGCCAAGAAAAACAGCCCTGTCGTAGAGGTGTGGGACAAGAAGACAGAA AAACTCTGTGAACTAATAGACTGTGTGCACTTCTTAAAGGAGGTGATGGTGAAAATAAACAAGGACTCGA AGCACAAGCTGTCCTACTCTGGGAGGGTGAAGGCACTCTGCCTGCAGAAGAACACAGCTCTCTGGATCGG AACTGGAGGAGGCCACATCTTACTCCTGGATCTTTCTACACGGCGAGTCATCCGCACCATCCACAATTTC TGTGATTCCGTGAGAGCCATGGCCACAGCTCAGTTAGGCAACCTTAAAAATGTCATGCTGGTTTTGGGGT ACAAGCGGAAGAGTACAGAAGGAACCCAAGAACAAAAAGAGATACAATCTTGTTTGTCTATTTGGGACCT CAATCTTCCACATGAAGTGCAAAACTTAGAAAAACACATTGAAGTAAGAACAGAACTGGCTGATAAAATG AGGAAAACATCTGTCGAATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191789
Insert Size	7581 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001191789.1, NP_001178718.1
RefSeq Size	7581 bp
RefSeq ORF	7581 bp
Locus ID	300160

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