ZNF268 (NM_003415) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HZF3
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_003415, the custom clone sequence may differ by one or more nucleotides ATGGCCACCAGGGTCCGGACAGCTTCTATTTGGGTCCCACCTCTCCAAGAACGAAACAGTTCATGGGATA GGATCAGAAAGCTCCAAGGTCAGGAATCCATCTTGGGCCAAGGGACTCCTGGTCTGCAACCTCTCCCTGG AACACCCAGGCAGAAGCAGAAGAGTCGCAGAATAGAGAAAGTCCTAGAGTGGCTGTTTATTTCCCAAGAG CAGCCAAAAATCACCAAGTCCTGGGGACCTTTGTCATTCATGGATGTGTTTGTGGATTTTACCTGGGAGG AGTGGCAGCTGCTAGACCCAGCACAGAAGTGCCTGTACAGGAGTGTGATGTTGGAGAACTATAGCAACCT GGTGTCCCTAGGGTACCAACACACCAAACCTGATATCATCTTCAAGTTGGAACAAGGAGAAGAGCTGTGT ATGGTGCAGGCCCAAGTTCCAAATCAGACCTGTCCAAACACAGTCTGGAAAATTGATGATCTTATGGATT GGCATCAGGAAAATAAAGACAAGCTGGGAAGTACGGCAAAAAGCTTTGAATGCACTACATTTGGAAAACT ATGTCTTCTTAGTACAAAGTATCTTTCAAGACAAAAACCTCATAAATGTGGCACGCATGGAAAGAGTTTG AAATATATAGATTTCACTAGTGATTATGCTAGAAATAATCCTAATGGGTTTCAGGTACATGGAAAATCAT TCTTCCATTCTAAACATGAGCAAACTGTTATTGGAATAAAATACTGTGAAAGTATTGAATCTGGAAAAAC CGTCAATAAGAAATCGCAACTTATGTGCCAACAAATGTATATGGGCGAAAAACCCTTTGGATGCAGCTGT TGTGAGAAAGCCTTCAGCAGCAAGTCATACCTTCTAGTGCATCAGCAAACTCATGCCGAAGAGAAACCCT ATGGTTGTAATGAATGTGGGAAAGACTTCAGTAGTAAATCATACCTCATTGTACATCAGAGAATTCATAC AGGAGAGAAACTACATGAATGCAGTGAATGCAGGAAAACATTCAGTTTCCATTCACAGCTTGTTATACAT CAGAGAATTCACACAGGTGAGAATCCCTATGAGTGCTGTGAATGTGGGAAAGTCTTCAGTAGGAAAGACC AGCTTGTTTCACACCAGAAAACTCATTCAGGACAGAAACCATATGTGTGTAATGAATGTGGGAAAGCTTT TGGTTTAAAATCACAGCTCATTATACATGAAAGAATTCATACAGGAGAGAAACCATATGAATGCAATGAA TGTCAGAAAGCCTTTAATACAAAGTCAAACCTTATGGTACATCAGAGAACCCATACAGGGGAGAAACCTT ATGTTTGTAGTGATTGTGGAAAAGCCTTTACATTCAAGTCACAGCTCATTGTACATCAGGGGATTCACAC AGGAGTAAAGCCCTATGGGTGTATTCAGTGTGGTAAAGGATTCAGTTTGAAATCACAGCTCATTGTACAT CAGAGAAGTCACACAGGAATGAAACCTTATGTATGCAATGAATGTGGCAAAGCCTTCAGGAGCAAGTCAT ACCTTATTATACATACAAGGACTCATACAGGAGAAAAACTCCATGAATGCAACAATTGTGGGAAAGCCTT CAGTTTTAAATCACAGCTCATTATACATCAGAGGATTCATACAGGAGAGAACCCCTATGAATGCCATGAA TGTGGGAAAGCCTTCAGTCGGAAATACCAGCTTATTTCACACCAGAGAACTCATGCAGGAGAGAAGCCTT ATGAATGCACCGACTGTGGAAAGGCTTTTGGTTTAAAGTCACAGCTTATTATACACCAGAGAACTCATAC AGGGGAGAAACCATTTGAATGTAGTGAGTGTCAGAAAGCCTTTAATACAAAGTCAAACCTGATTGTACAT CAGAGAACTCATACAGGAGAGAAACCCTATAGTTGTAATGAATGTGGAAAAGCCTTTACGTTCAAATCAC AGCTCATTGTACATAAAGGAGTGCACACTGGAGTAAAACCCTATGGATGCAGTCAATGTGCAAAAACCTT TAGTTTGAAGTCCCAGCTCATTGTACATCAGAGAAGTCACACAGGAGTAAAACCATATGGATGCAGTGAG TGTGGGAAAGCCTTCAGGAGCAAGTCATACCTTATTATACATATGAGAACTCATACAGGAGAGAAACCAC ATGAGTGCAGGGAATGCGGGAAATCCTTTAGTTTCAATTCACAACTCATTGTGCATCAGAGAATTCACAC AGGAGAAAATCCCTATGAATGCAGTGAATGTGGGAAAGCCTTTAATAGGAAAGACCAGCTCATTTCACAT CAGCGAACTCATGCAGGGGAAAAGCCTTATGGGTGCAGTGAATGTGGGAAAGCTTTTAGCAGCAAGTCAT ACCTAATTATACACATGAGAACTCATTCAGGTGAAAAACCATATGAATGTAATGAATGTGGGAAAGCCTT CATTTGGAAATCACTACTCATTGTACATGAGCGAACTCATGCAGGGGTCAACCCTTATAAATGCAGTCAA TGTGAGAAATCCTTCAGTGGGAAATTACGCCTTCTTGTACACCAGAGAATGCACACAAGAGAGAAACCAT ATGAATGCAGTGAGTGTGGAAAAGCCTTCATTAGGAATTCTCAACTCATTGTACATCAAAGAACTCATTC AGGAGAGAAACCCTATGGGTGCAATGAATGTGGGAAAACCTTCTCTCAAAAATCAATTCTCAGTGCACAT CAGAGAACACATACAGGAGAGAAGCCTTGTAAGTGCACTGAATGTGGGAAAGCCTTTTGTTGGAAGTCAC AGCTCATTATGCATCAGAGAACTCATGTAGATGACAAACATTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_003415 unedited GTGTTCAACATTATGTNATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGATT GTTCCGCGCCGATGGCGAGATCCTTGTTCCTCAGATAGCGTTCATCGCCCGTCGTGGTCA ACGGGCCAGCCGAGTCTGGAGTGGTTGCGAACCCTTCTGGCTGCAGATCTGGAGGTGGAG GCAGTACCCTGGACTCTATTCTGCTGCCCCTTCAGGGTTTGGAGGAGCCGGAGCATCCCT CGCGTCCTGTCACTTCCAGCGAGGCACACAAAACTGACCGTAGGGATGGCCACCAGGGTC CGGACAGCTTCTATTTGGGTCCCACCTCTCCAAGAACGAAACAGTTCATGGGATAGGATC AGAAAGCTCCAAGGTCAGGAATCCATCTTGGGCCAAGGGACTCCTGGTCTGCAACCTCTC CCTGGAACACCCAGGCAGAAGCAGAAGAGTCGCAGAATAGAGAAAGTCCTAGAGTGGCTG TTTATTTCCCAAGAGCAGCCAAAAATCACCAAGTCCTGNGGACCTTTGTCATTCATGGAT GTGTTTGTGGATTTTACCTGNGAGGAGTGGCAGCTGCTAGACCCAGCACAGAAGTGCCTG TACAGGAGTGTGATGTTGGAGAACTATAGCAACCTGGTGTCCCTAGGGTACCAACACACC AAACCTGATATCATCTTCAAGTTGGAACAANGAGAAGAGCTGTGTATGGTGCANGCCCAA GTTCCAAATAGACCTGTCCAACACAGTCTGGAAAATGATGATCTTATGGATTGGCATCAA GAAAATAAGACACGCTGGAAGTACCGCAAAAAGCTTTGATGCACTANCATTTGAAAACTA TGTCTTCTTTATACAAAGTATCTTTCAAGAAAAACCTCATAAATGTGCCCGCATGGAAAG AAGTTGAATATATAGATTTCACTAGTGATTATGCTAAA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_003415 unedited GAACCGCGGCCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA ATAAAACTTCTAAGGTGAGGTTAACAGTCCAGCTGTGGTTTATTTGGTACATTCATAAGA TGTTCACTCCTATATATATTTTTTCCCTAGGGGTTTCTCATTTCTAGATTTACACTTTCC CTCATAGCATGGTCCCTCCAAGGTATATGCTTCCGATACATAAAATGTTTGTAATTTTGT TAATTCTGCCATTCATTAGCTCAATAATTTCTCTCATTAAAATTCTGTAAACTATGAATT TCAAAGAAGTCTATTCTATATTCATCTGGTTCATGAAGTTTCCTCTTTGTGAATCTTATG TTAGTAAAATGACTTTACAACGTTTAACATGGTTCCTCACCTGTATGAACTTTTTAAAAA AAGGTATGACTTGCTGCTAAAAATTTTCAGAGTGGTTACTAAATTCAGTTTTCCTGTGTA TTGTCTACTATCTTTTTGCATGTAAAGAACAGCTTTCTGTGGATGCCTTTCCATATGCTG TGCATTCATAAAGTTCTATTCAGTATGAGTTTTCTGGAAGAGACAAAATGATTCCACTTA CAGAGTTTCTCTTTATATATGATTGTTTCTATCTCTTGTGAATCCACTTTTCAGAGTTTC GTAAAATTATCAATGTTTGTCATCTACATGAGTTCTCTGATGCATAATGAGCTGTGACTT CCAACAAAAGGCTTTCCCACATTCAGTGCACTTACNAAATTTCTCTCCTGTATGTGTTCT CTGATGTGCACTGAGAAATGATTTTTGAGAGAAAGTTTTCCCACATTCATTGCACCCCTA GGGTATCTCTCCTGAATGAGTTCTTTGATGTACATGAGTTGAGAATCCCAATGAAGGCTT TTCCCACTCACTGCATTCATATGGTTCTCTCCTGGGGCCTCCCTTGGGTTCAGAAAGACG TATTTTCCCCTGAAGATTCTCACATGACTGCGTTATTAAGTTGGACCC
Restriction Sites	NotI-NotI
ACCN	NM_003415
Insert Size	4000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_003415.1, NP_003406.1
RefSeq Size	5756 bp
RefSeq ORF	2844 bp
Locus ID	10795
UniProt ID	Q14587
Domains	KRAB, zf-C2H2
Protein Families	Transcription Factors
Gene Summary	Isoform 1: Acts as a transcriptional repressor. Inhibits erythroid differentiation and tumor cell proliferation. Plays a role during ovarian cancer development and progression.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longest isoform (a). Variants 1 and 2 both encode isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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