United States
Germany
Japan
United Kingdom
China
VAV1 (NM_005428) Human Untagged Clone
CAT＃: SC116729
VAV1 (untagged)-Human vav 1 guanine nucleotide exchange factor (VAV1)
CNY 9,296.00
货期*
现货
规格
| Cited in 1 publication. |
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | VAV |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC116729 sequence for NM_005428 edited (data generated by NextGen Sequencing)
ATGGAGCTGTGGCGCCAATGCACCCACTGGCTCATCCAGTGCCGGGTGCTGCCGCCCAGC CACCGCGTGACCTGGGATGGGGCTCAGGTGTGTGAACTGGCCCAGGCCCTCCGGGATGGT GTCCTTCTGTGTCAGCTGCTTAACAACCTGCTACCCCATGCCATCAACCTGCGTGAGGTC AACCTGCGCCCCCAGATGTCCCAGTTCCTGTGCCTTAAGAACATTAGAACCTTCCTGTCC ACCTGCTGTGAGAAGTTCGGCCTCAAGCGGAGCGAGCTCTTCGAAGCCTTTGACCTCTTC GATGTGCAGGATTTTGGCAAGGTCATCTACACCCTGTCTGCTCTGTCCTGGACCCCGATC GCCCAGAACAGGGGGATCATGCCCTTCCCCACCGAGGAGGAGAGTGTAGGTGATGAAGAC ATCTACAGTGGCCTGTCCGACCAGATCGACGACACGGTGGAGGAGGATGAGGACCTGTAT GACTGCGTGGAGAATGAGGAGGCGGAAGGCGACGAGATCTATGAGGACCTCATGCGCTCG GAGCCCGTGTCCATGCCGCCCAAGATGACAGAGTATGACAAGCGCTGCTGCTGCCTGCGG GAGATCCAGCAGACGGAGGAGAAGTACACTGACACGCTGGGCTCCATCCAGCAGCATTTC TTGAAGCCCCTGCAACGGTTCCTGAAACCTCAAGACATTGAGATCATCTTTATCAACATT GAGGACCTGCTTCGTGTTCATACTCACTTCCTAAAGGAGATGAAGGAAGCCCTGGGCACC CCTGGCGCAGCCAATCTCTACCAGGTCTTCATCAAATACAAGGAGAGGTTCCTCGTCTAT GGCCGCTACTGCAGCCAGGTGGAGTCAGCCAGCAAACACCTGGACCGTGTGGCCGCAGCC CGGGAGGACGTGCAGATGAAGCTGGAGGAATGTTCTCAGAGAGCCAACAACGGGAGGTTC ACCCTGCGGGACCTGCTGATGGTGCCTATGCAGCGAGTTCTCAAATATCACCTCCTTCTC CAGGAGCTGGTGAAACACACGCAGGAGGCGATGGAGAAGGAGAACCTGCGGCTGGCCCTG GATGCCATGAGGGACCTGGCTCAGTGCGTGAACGAGGTCAAGCGAGACAACGAGACACTG CGACAGATCACCAATTTCCAGCTGTCCATTGAGAACCTGGACCAGTCTCTGGCTCACTAT GGCCGGCCCAAGATCGACGGGGAACTCAAGATCACCTCGGTGGAACGGCGCTCCAAGATG GACAGGTATGCCTTCCTGCTCGACAAAGCTCTACTCATCTGTAAGCGCAGGGGAGACTCC TATGACCTCAAGGACTTTGTAAACCTGCACAGCTTCCAGGTTCGGGATGACTCTTCAGGA GACCGAGACAACAAGAAGTGGAGCCACATGTTCCTCCTGATCGAGGACCAAGGTGCCCAG GGCTATGAGCTGTTCTTCAAGACAAGAGAATTGAAGAAGAAGTGGATGGAGCAGTTTGAG ATGGCCATCTCCAACATCTATCCGGAGAATGCCACCGCCAACGGGCATGACTTCCAGATG TTCTCCTTTGAGGAGACCACATCCTGCAAGGCCTGTCAGATGCTGCTTAGAGGTACCTTC TATCAGGGCTACCGCTGCCATCGGTGCCGGGCATCTGCACACAAGGAGTGTCTGGGGAGG GTCCCTCCATGTGGCCGACATGGGCAAGATTTCCCAGGAACTATGAAGAAGGACAAACTA CATCGCAGGGCTCAGGACAAAAAGAGGAATGAGCTGGGTCTGCCCAAGATGGAGGTGTTT CAGGAATACTACGGGCTTCCTCCACCCCCTGGAGCCATTGGACCCTTTCTACGGCTCAAC CCTGGAGACATTGTGGAGCTCACGAAGGCTGAGGCTGAACAGAACTGGTGGGAGGGCAGA AATACATCTACTAATGAAATTGGCTGGTTTCCTTGTAACAGGGTGAAGCCCTATGTCCAT GGCCCTCCTCAGGACCTGTCTGTTCATCTCTGGTACGCAGGCCCCATGGAGCGGGCAGGG GCAGAGAGCATCCTGGCCAACCGCTCGGACGGGACTTTCTTGGTGCGGCAGAGGGTGAAG GATGCAGCAGAATTTGCCATCAGCATTAAATATAACGTCGAGGTCAAGCACATTAAAATC ATGACAGCAGAAGGACTGTACCGGATCACAGAGAAAAAGGCTTTCCGGGGGCTTACGGAG CTGGTGGAGTTTTACCAGCAGAACTCTCTAAAGGATTGCTTCAAGTCTCTGGACACCACC TTGCAGTTCCCCTTCAAGGAGCCTGAAAAGAGAACCATCAGCAGGCCAGCAGTGGGAAGC ACAAAGTATTTTGGCACAGCCAAAGCCCGCTATGACTTCTGCGCCCGAGACCGATCAGAG CTGTCGCTCAAGGAGGGTGACATCATCAAGATCCTTAACAAGAAGGGACAGCAAGGCTGG TGGCGAGGGGAGATCTATGGCCGGGTTGGCTGGTTCCCTGCCAACTACGTGGAGGAAGAT TATTCTGAATACTGCTGA Clone variation with respect to NM_005428.2 >OriGene 5' read for NM_005428 unedited
CACGAGAATGGACTGTGGCGCCAATGCACCCACTGGCTCATCCAGTGCCGGGTGCTGCCG CCCAGCCACCGCGTGACCTGGGATGGGGCTCAGGTGTGTGAACTGGCCCAGGCCCTCCGG GATGGTGTCCTTCTGTGTCAGCTGCTTAACAACCTGCTACCCCATGCCATCAACCTGCGT GAGGTCAACCTGCGCCCCCAGATGTCCCAGTTCCTGTGCCTTAAGAACATTAGAACCTTC CTGTCCACCTGCTGTGAGAAGTTCGGCCTCAAGCGGAGCGAGCTCTTCGAAGCCTTTGAC CTCTTCGATGTGCAGGATTTTGGCAAGGTCATCTACACCCTGTCTGCTCTGTCCTGGACC CCGATCGCCCAGAACAGGGGGATCATGCCCTTCCCCACCGAGGAGGAGAGTGTAGGTGAT GAAGACATCTACAGTGGCCTGTCCGACCAGATCGACGACACGGTGGAGGAGGATGAGGAC CTGTATGACTGCGTGGAGAATGAGGAGGCGGAAGGCGACGAGATCTATGAGGACCTCATG CGCTCGGAGCCCGTGTCCATGCCGCCCAAGATGACAGAGTATGACAAGCGCTGCTGCTGC CTGCGGGAGATCCAGCAGACGGAGGAGAAGTACACTGACACGCTGGGCTCCATCCAGCAG CATTTCTTGAAGCCCCTGNCACGGTTCCTGAAACCTCAAG >OriGene 3' read for NM_005428 unedited
TTTTTGGGTTTGACTATGNNACNCGCGGCCGCATACTAGNGATCGATTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATATCCACCTTCATCCTTTTATTAA ATGGAACCCCCTTGAGCCCCGTTTGCTTGAGGGGACCCAAAAGAGAAATCGGGGTGTTAT AAATTAACCATGTCAGGGCACATCCCGGGAGCACCGCCAGCTGGACACTGGCCTCCTCCG TCCATCCCAAAATCTCCACCCGCCGGGAGCTGTCACAACCCCTGGGTCCGCTGCCTGCCC ACCCCGGGCTCAAAACCTGGAGTTTCTCGTCTCTTTGCCAAGGCCCCAGGGCTCAACAGT TTTCAAAATAATCTTCCTCCACGTAGTTGGCAGGGAACCAGCCAACCCGGCCATAGATTT CCCCTTGCCACCAACCTTGGTGTCCCTTTTTGTTAAGGATCTTGAAGAAGTCCCCCTCCT TGAGCCACAGCTCTGATACGGTCTCGGGCGCAAAAATATAGAGGGCTTTGGCTGTGCCAA AATACTTTGGGCTTCCCACTGGTGGCCTGGTGAAGGTTCTCTTTTCAGGCTCCTTGAAGG GGAACAGGAAGGTGGGTGTCCAAAAACTTGGAGCAACCCTTTAAAGAGTTCTGCCGGTAA AACACCACCAACTCCGTAAGCCCCCGGAAAACCTTTTTCTCTGTGATCCGGGACAATCCC TTTGGAGCCAGAATTTAAAGGGCCTGGCCCCGAACTTTTTTTTAATGGTGAAGGGAAAAT TTGTGGGTTCCTTAACCTTTTGGCGGACCAGAAAAACCCCGTTCTAAAGGGTGGGCCAGA AGCTTTTGGCCCTGGCCCCCCCCAGGGGGGCCGGGTACACAAAAATAC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_005428 |
| Insert Size | 2800 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005428.2, NP_005419.2 |
| RefSeq Size | 2888 bp |
| RefSeq ORF | 2538 bp |
| Locus ID | 7409 |
| UniProt ID | P15498 |
| Domains | RhoGEF, SH2, SH3, CH, PH, DAG_PE-bind |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | B cell receptor signaling pathway, Chemokine signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Leukocyte transendothelial migration, Natural killer cell mediated cytotoxicity, Regulation of actin cytoskeleton, T cell receptor signaling pathway |
| Gene Summary | This gene is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. The encoded protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. The encoded protein has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Apr 2012] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Identification of the Catalytic Site of Phospholipase D2 (PLD2) Newly Described Guanine Nucleotide Exchange Factor Activity
,Madhu Mahankali, Karen M. Henkels, Gerald Alter, and Julian Gomez-Cambronero,
J. Biol. Chem., Nov 2012; 287: 41417 - 41431.
[VAV1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224806 | VAV1 (Myc-DDK-tagged)-Human vav 1 guanine nucleotide exchange factor (VAV1) |
CNY 9,280.00 |
|
| RC224806L3 | Lenti ORF clone of Human vav 1 guanine nucleotide exchange factor (VAV1), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC224806L4 | Lenti ORF clone of Human vav 1 guanine nucleotide exchange factor (VAV1), mGFP tagged |
CNY 9,030.00 |
|
| RG224806 | VAV1 (tGFP-tagged) - Human vav 1 guanine nucleotide exchange factor (VAV1) |
CNY 10,880.00 |
Customer
Reviews
Loading...
