REV3L (NM_002912) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	POLZ; REV3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC318746 representing NM_002912. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTTTTCAGTAAGGATAGTGACTGCAGACTACTACATGGCCAGCCCGCTGCAGGGGCTGGATACCTGC CAATCCCCCCTCACCCAGGCCCCTGTCAAGAAGGTGCCGGTGGTGCGAGTCTTCGGAGCGACCCCGGCA GGTCAGAAGACATGTCTTCATCTACATGGCATCTTTCCTTACCTCTATGTGCCATACGATGGTTATGGA CAGCAGCCAGAAAGCTATCTTTCTCAGATGGCATTCAGTATCGACAGAGCACTTAATGTGGCTTTAGGC AATCCATCTTCCACTGCTCAGCATGTGTTCAAAGTGTCATTAGTATCAGGAATGCCTTTTTATGGTTAT CATGAGAAGGAAAGACACTTTATGAAGATCTATCTTTACAATCCTACAATGGTGAAAAGGATATGTGAA CTTTTGCAAAGCGGAGCCATAATGAATAAATTTTACCAGCCTCATGAAGCGCATATTCCCTACCTCCTA CAGCTCTTCATTGACTACAATCTTTATGGCATGAATTTAATAAATCTGGCTGCTGTCAAGTTCCGAAAA GCAAGAAGGAAAAGTAATACATTGCATGCAACTGGATCCTGCAAGAATCATTTATCAGGAAATTCTCTT GCTGATACTTTATTTCGGTGGGAACAAGATGAAATACCAAGCTCTTTAATATTGGAAGGTGTTGAACCA CAGAGTACATGTGAATTAGAAGTGGATGCTGTAGCTGCTGATATCTTAAATCGTCTGGACATTGAAGCT CAAATTGGTGGAAACCCTGGTCTACAGGCCATATGGGAAGATGAAAAGCAACGGCGAAGAAACAGAAAT GAAACTTCTCAAATGAGCCAACCTGAGTCACAAGATCACAGGTTTGTGCCAGCAACAGAAAGTGAAAAA AAATTTCAGAAGAGACTTCAGGAAATTCTCAAACAGAATGATTTCTCTGTAACATTATCAGGATCTGTG GACTACAGCGATGGATCCCAGGAGTTCTCTGCTGAGTTAACATTGCACTCTGAGGTTCTGTCTCCTGAA ATGCTTCAGTGTACACCAGCCAATATGGTAGAAGTTCACAAAGACAAAGAGTCAAGCAAAGGTCACACT AGACACAAAGTGGAAGAAGCTCTTATTAATGAAGAAGCAATTTTGAACCTTATGGAAAATAGTCAGACT TTTCAGCCTTTGACCCAAAGACTGAGTGAGTCACCTGTTTTCATGGACAGTAGTCCTGATGAGGCTCTG GTACATCTTCTTGCTGGTTTGGAAAGTGATGGATATCGGGGGGAAAGAAATAGGATGCCATCACCATGT CGCTCCTTTGGAAATAATAAATATCCACAAAATAGTGATGATGAAGAAAATGAACCACAGATTGAAAAA GAGGAAATGGAGCTTAGTTTGGTGATGTCCCAGAGATGGGACAGCAATATTGAAGAACATTGTGCCAAA AAGAGATCACTGTGCAGAAATACCCACAGAAGTTCAACTGAAGATGATGACTCATCTTCAGGAGAAGAA ATGGAATGGAGTGATAACAGTTTGCTTCTAGCCAGTCTTTCTATACCTCAGTTAGATGGAACTGCAGAT GAAAATAGTGACAATCCATTGAACAATGAAAATTCTAGAACCCACTCTTCTGTAATTGCAACAAGCAAG CTTTCAGTTAAACCCTCCATCTTTCACAAAGATGCTGCTACATTAGAACCCTCATCTTCTGCTAAGATT ACCTTTCAGTGTAAACACACAAGTGCCCTTTCTTCCCATGTTTTGAACAAGGAAGATTTAATTGAAGAC CTTTCACAGACAAACAAAAATACAGAAAAAGGTCTAGATAACTCAGTCACTTCTTTTACAAACGAAAGC ACTTATTCTATGAAATACCCTGGATCTTTAAGCAGTACTGTTCATTCAGAAAATTCTCATAAAGAGAAT AGTAAGAAAGAGATCCTCCCAGTATCTTCCTGTGAAAGTAGTATTTTTGATTATGAAGAAGATATTCCA TCTGTTACAAGACAAGTACCAAGTAGAAAATATACAAACATTAGAAAAATCGAAAAGGATTCCCCTTTT ATACATATGCACCGTCACCCTAACGAGAATACATTGGGCAAAAATTCTTTCAACTTTTCTGACTTAAAT CATTCAAAAAATAAAGTATCCTCTGAAGGAAATGAAAAAGGAAACAGCACAGCTCTGAGTAGTTTATTC CCTTCATCATTTACTGAAAATTGTGAATTACTGTCATGCTCAGGGGAGAATAGAACTATGGTGCATTCT CTTAATAGCACTGCTGATGAAAGTGGACTAAATAAACTTAAAATTAGGTATGAAGAATTTCAAGAACAT AAAACAGAAAAGCCAAGCCTCAGCCAGCAAGCAGCACACTATATGTTTTTTCCCAGTGTTGTTCTTTCT AACTGTCTTACTAGACCACAGAAACTATCTCCTGTCACATATAAATTACAACCTGGCAATAAACCATCC CGGTTAAAATTGAATAAAAGGAAACTTGCAGGTCATCAGGAGACTTCTACCAAAAGTAGTGAGACTGGA TCCACAAAAGATAATTTTATACAAAATAATCCTTGTAATAGTAATCCTGAGAAGGATAATGCATTGGCT AGTGATTTAACTAAAACCACTCGTGGAGCTTTTGAAAATAAAACACCCACAGATGGTTTTATAGACTGT CACTTTGGAGATGGAACGTTAGAAACTGAGCAGTCCTTTGGACTATATGGAAATAAATACACACTTAGA GCCAAACGCAAGGTAAATTATGAGACTGAAGACAGTGAGTCAAGTTTTGTAACTCACAACTCAAAAATT AGTCTACCTCATCCCATGGAAATTGGTGAAAGTTTAGATGGAACTCTCAAATCCCGAAAACGAAGAAAA ATGTCTAAAAAGCTGCCCCCTGTCATCATAAAGTATATTATTATTAATAGATTTAGAGGGAGAAAAAAT ATGCTTGTGAAGCTAGGAAAAATAGACTCTAAAGAAAAACAAGTAATATTAACAGAAGAAAAAATGGAA CTATATAAAAAGCTTGCACCTTTGAAGGACTTTTGGCCAAAAGTTCCCGACTCCCCTGCAACCAAATAT CCCATTTATCCACTAACACCAAAGAAAAGTCACAGAAGAAAGTCAAAACATAAATCTGCTAAGAAAAAA ACTGGTAAACAACAAAGGACAAATAATGAAAATATTAAAAGAACTTTGTCTTTCAGGAAAAAACGGTCA CATGCTATTCTTTCTCCTCCCTCACCATCTTACAATGCTGAAACCGAAGATTGTGACCTGAATTATAGT GATGTTATGTCTAAACTAGGTTTTCTTTCTGAGAGAAGCACAAGTCCCATAAATTCTTCTCCACCTCGC TGCTGGTCTCCCACAGATCCAAGAGCTGAAGAAATCATGGCTGCTGCAGAAAAAGAGGCAATGCTTTTT AAGGGTCCTAATGTATATAAGAAGACTGTTAATTCTCGTATAGGAAAAACTAGTCGCGCAAGAGCACAG ATTAAGAAATCAAAAGCAAAGCTTGCTAATCCCTCTATAGTTACTAAGAAAAGGAACAAACGAAATCAG ACAAATAAACTAGTAGATGATGGAAAAAAGAAACCAAGAGCAAAACAAAAAACAAATGAGAAAGGTACA TCGAGAAAGCATACAACACTTAAGGATGAAAAAATAAAATCTCAGTCTGGTGCTGAGGTTAAGTTTGTA CTGAAACACCAGAATGTGTCTGAATTTGCAAGTAGTTCTGGAGGCTCTCAACTACTTTTTAAACAGAAA GATATGCCACTAATGGGCTCTGCTGTAGATCATCCCCTTTCTGCTTCCCTACCCACTGGAATTAATGCA CAACAGAAGTTATCTGGCTGCTTTTCTTCTTTCTTAGAAAGCAAGAAGTCTGTAGATTTGCAGACATTC CCCAGTTCACGAGATGATTTGCATCCATCAGTTGTTTGTAATTCTATAGGACCTGGAGTCTCAAAAATT AATGTTCAAAGGCCTCATAATCAAAGTGCTATGTTTACTCTAAAGGAATCAACGTTAATTCAAAAAAAT ATATTTGACCTTTCCAATCATTTATCTCAGGTAGCACAGAATACACAGATATCTTCTGGTATGTCCTCA AAGATAGAAGATAATGCAAATAATATACAAAGAAACTATTTGTCATCAATCGGAAAGTTAAGTGAATAT CGCAATTCCCTAGAATCAAAGCTGGACCAAGCATATACCCCTAATTTTTTGCATTGCAAAGACAGTCAG CAGCAGATTGTGTGCATAGCGGAACAGTCAAAGCACAGTGAAACTTGTTCTCCGGGAAATACAGCTTCA GAGGAAAGCCAAATGCCTAATAATTGCTTTGTAACTTCCTTGAGAAGTCCAATCAAACAAATAGCATGG GAGCAAAAGCAAAGGGGCTTTATTTTAGATATGTCAAATTTTAAACCTGAAAGAGTAAAACCGAGGTCG TTATCAGAAGCAATTTCACAAACCAAAGCACTTTCTCAGTGTAAAAATCGAAATGTGTCAACACCTTCA GCATTTGGTGAAGGACAGTCTGGACTGGCAGTTCTAAAAGAATTGTTACAAAAAAGACAGCAGAAAGCA CAAAATGCAAATACTACACAAGACCCATTATCCAATAAACATCAACCAAATAAAAATATTTCTGGTTCC CTTGAGCATAACAAAGCAAATAAACGGACACGATCGGTAACGTCCCCAAGAAAACCTCGAACTCCCAGA AGTACAAAACAAAAAGAAAAAATCCCCAAACTTCTCAAAGTAGACTCTTTAAATTTACAAAACTCTAGC CAGTTGGATAACTCTGTATCAGATGATAGTCCCATCTTTTTTTCAGATCCAGGCTTTGAAAGTTGTTAC TCACTTGAAGATAGTTTATCTCCTGAACATAATTATAATTTTGATATTAACACAATAGGTCAGACTGGA TTTTGTAGCTTTTATTCTGGAAGTCAGTTTGTCCCAGCTGATCAGAATTTGCCTCAGAAGTTCCTAAGT GATGCTGTTCAGGATCTTTTTCCAGGACAAGCTATAGAAAAAAATGAGTTTTTAAGTCATGACAACCAG AAATGTGATGAAGACAAGCATCATACCACAGACTCAGCCTCATGGATTAGATCTGGTACTTTAAGTCCT GAAATTTTTGAGAAGTCAACCATAGATAGCAATGAGAATCGTCGCCACAACCAGTGGAAAAATAGCTTT CATCCTCTAACAACTCGGTCTAACTCAATAATGGATTCTTTCTGTGTTCAGCAGGCAGAAGACTGTCTA AGTGAAAAATCTAGATTGAATAGGAGTTCAGTAAGCAAAGAAGTGTTTCTTAGCCTCCCACAGCCAAAC AATTCAGACTGGATTCAAGGTCACACCAGAAAAGAAATGGGACAGTCTCTTGACTCAGCCAATACCTCT TTTACTGCAATACTCTCCTCCCCTGATGGTGAACTTGTAGACGTGGCCTGTGAAGATTTAGAACTGTAT GTTTCAAGAAACAATGATATGTTGACACCAACTCCTGATAGTTCACCAAGATCTACTAGCTCTCCTTCA CAATCTAAAAATGGCAGCTTCACCCCTCGAACTGCTAACATTCTGAAACCACTTATGTCCCCCCCAAGT AGGGAAGAAATTATGGCAACTTTGTTGGATCATGACCTGTCTGAGACTATTTACCAGGAACCATTTTGC AGTAATCCTTCTGATGTACCAGAAAAGCCCAGGGAGATTGGTGGACGGCTCCTCATGGTAGAAACTCGA CTTGCAAATGATCTGGCTGAGTTTGAGGGAGACTTTTCCTTGGAAGGACTTCGTCTTTGGAAAACAGCA TTCTCAGCAATGACTCAGAATCCAAGGCCAGGGTCACCCCTTCGCAGTGGCCAAGGAGTTGTCAATAAA GGGTCAAGTAATAGCCCTAAGATGGTTGAAGATAAAAAAATTGTGATTATGCCTTGCAAATGTGCCCCA AGTCGACAACTGGTTCAAGTGTGGCTTCAAGCCAAAGAAGAATACGAACGTTCCAAGAAACTGCCTAAA ACCAAGCCAACTGGAGTTGTAAAATCTGCTGAGAACTTTAGCTCTTCAGTTAACCCAGATGACAAACCT GTAGTGCCTCCAAAAATGGATGTAAGTCCATGTATACTCCCCACTACAGCACATACCAAGGAGGATGTT GATAATTCTCAGATTGCTTTACAAGCACCAACCACGGGATGTAGTCAAACTGCAAGTGAAAGTCAGATG CTGCCACCAGTTGCCTCTGCAAGTGATCCCGAAAAAGATGAAGATGATGATGATAACTATTACATTAGT TATAGCTCCCCTGATTCTCCAGTAATTCCCCCTTGGCAACAACCAATATCCCCAGATTCCAAAGCATTA AATGGAGATGATAGACCCTCATCACCAGTAGAGGAGCTGCCTTCATTGGCTTTTGAGAACTTCTTAAAG CCAATAAAAGATGGTATACAAAAAAGCCCCTGCAGTGAGCCTCAAGAGCCTCTAGTGATATCTCCAATT AATACTAGGGCAAGAACTGGGAAATGTGAATCACTTTGCTTTCATAGTACACCAATCATACAGAGAAAA CTTCTGGAAAGGCTTCCTGAAGCACCTGGCCTTAGCCCATTATCAACAGAACCAAAAACACAGAAGTTG AGTAATAAGAAAGGAAGTAATACTGACACTCTTAGAAGAGTACTGTTAACACAAGCAAAGAATCAATTT GCAGCAGTAAATACCCCACAGAAAGAAACTTCTCAGATTGATGGACCATCTTTAAACAATACTTACGGT TTCAAAGTCAGCATACAAAACTTACAGGAGGCAAAAGCTTTACATGAGATACAAAATCTTACCCTAATC AGTGTGGAGTTGCATGCTCGAACTAGACGAGACTTAGAACCGGATCCTGAATTTGACCCAATCTGTGCT CTGTTCTACTGCATCTCATCTGACACTCCACTGCCAGATACAGAAAAAACAGAACTCACAGGTGTAATA GTGATTGATAAAGACAAGACAGTTTTCAGTCAAGATATCAGATATCAGACTCCATTACTTATTAGATCT GGAATTACAGGACTCGAAGTCACCTATGCTGCTGATGAGAAGGCACTTTTTCATGAAATTGCAAATATA ATAAAGAGGTATGATCCTGATATTCTGCTAGGATATGAGATTCAGATGCATTCCTGGGGTTACCTCTTA CAAAGGGCTGCCGCTTTAAGTATTGACTTATGTCGGATGATCTCTCGGGTGCCAGATGACAAAATTGAG AACAGATTTGCAGCTGAAAGAGATGAGTATGGATCATATACAATGAGTGAGATAAATATTGTTGGCCGA ATTACACTAAATCTTTGGAGAATCATGAGAAATGAGGTGGCTCTAACTAACTACACCTTTGAAAATGTG AGCTTTCATGTTCTTCATCAGCGTTTTCCCCTCTTTACCTTTCGAGTCTTGTCAGACTGGTTTGATAAC AAGACAGATCTATACAGATGGAAAATGGTTGATCATTATGTTAGCCGTGTCCGTGGAAATCTCCAAATG TTAGAACAGCTGGACCTGATTGGGAAAACCAGTGAGATGGCTAGACTTTTTGGCATTCAGTTTTTACAT GTACTGACAAGGGGTTCACAGTACCGTGTGGAATCAATGATGTTGCGTATTGCTAAACCAATGAACTAT ATTCCTGTGACACCTAGTGTTCAGCAAAGATCCCAGATGAGAGCCCCACAGTGTGTTCCTCTAATTATG GAGCCTGAATCCCGCTTCTATAGCAACTCTGTTCTCGTTTTGGATTTCCAATCACTTTATCCTTCTATT GTGATTGCATATAACTACTGCTTTTCCACCTGCCTTGGCCATGTGGAGAACTTGGGAAAGTATGATGAG TTCAAATTTGGCTGTACCTCTCTGAGAGTACCTCCAGATTTACTTTACCAAGTTAGGCATGATATCACA GTGTCCCCCAATGGAGTAGCTTTTGTCAAGCCTTCAGTAAGAAAAGGTGTACTACCAAGAATGCTTGAA GAAATTTTGAAGACTAGATTTATGGTGAAGCAGTCAATGAAGGCTTACAAGCAAGACAGAGCCCTGTCA CGAATGCTTGATGCGCGTCAGTTGGGACTTAAGCTGATAGCAAATGTCACATTTGGCTATACATCTGCT AATTTTTCTGGGAGAATGCCATGCATTGAGGTTGGCGATAGTATTGTTCACAAAGCCAGAGAGACCTTG GAACGAGCTATTAAACTGGTGAATGATACCAAGAAATGGGGGGCTAGGGTTGTATATGGCGATACTGAC AGTATGTTTGTGCTACTGAAAGGAGCCACTAAGGAGCAGTCTTTTAAGATTGGTCAGGAAATTGCCGAA GCTGTAACTGCTACCAATCCTAAACCAGTGAAATTGAAGTTTGAAAAGGTATATTTGCCCTGTGTTTTA CAAACAAAAAAGAGGTATGTGGGTTACATGTATGAAACACTGGATCAGAAGGACCCAGTATTTGATGCA AAAGGAATAGAAACAGTCAGAAGAGATTCCTGCCCTGCTGTTTCTAAGATACTTGAGCGTTCTCTAAAG CTGCTATTTGAAACGAGAGATATAAGTCTAATTAAACAGTATGTTCAGCGACAATGTATGAAGCTTCTG GAAGGAAAGGCCAGCATACAAGACTTTATCTTTGCCAAGGAATACAGAGGAAGTTTTTCTTATAAACCA GGAGCTTGTGTGCCAGCCCTTGAACTTACAAGGAAAATGCTGACTTATGACCGGCGCTCTGAGCCTCAG GTTGGGGAGCGAGTGCCATACGTCATCATTTATGGGACCCCCGGAGTACCACTTATCCAGCTTGTAAGG CGCCCAGTGGAAGTCCTGCAGGACCCAACTCTGAGACTGAATGCTACTTACTATATTACCAAGCAAATC CTTCCACCCTTGGCAAGAATCTTCTCACTTATTGGTATTGATGTCTTCAGCTGGTATCATGAATTACCA AGGATCCATAAAGCTACCAGCTCCTCGCGAAGTGAACCTGAAGGGCGGAAAGGCACTATTTCACAATAT TTTACTACCTTACACTGTCCTGTGTGTGATGACCTAACTCAGCATGGCATCTGTAGTAAATGTCGGAGC CAACCTCAGCATGTTGCAGTCATCCTCAACCAAGAAATCCGGGAGTTGGAACGTCAACAGGAGCAACTT GTAAAGATATGCAAGAACTGTACAGGTTGCTTTGATCGACACATCCCATGTGTTTCTCTGAACTGCCCA GTACTTTTCAAACTCTCCCGAGTAAATAGAGAATTGTCCAAGGCACCATATCTCCGGCAGTTATTAGAC CAGTTTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_002912
Insert Size	9393 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_002912.4
RefSeq Size	10854 bp
RefSeq ORF	9393 bp
Locus ID	5980
UniProt ID	O60673
Domains	DNA_pol_B_exo, DNA_pol_B, POLBc
Protein Families	Druggable Genome
Protein Pathways	Metabolic pathways
MW	352.8 kDa
Gene Summary	The protein encoded by this gene represents the catalytic subunit of DNA polymerase zeta, which functions in translesion DNA synthesis. The encoded protein can be found in mitochondria, where it protects DNA from damage. Defects in this gene are a cause of Mobius syndrome. [provided by RefSeq, Jan 2017] Transcript Variant: This variant (1) encodes the longer isoform (a).

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