United States
Germany
Japan
United Kingdom
China
Eph receptor A5 (EPHA5) (NM_004439) Human Untagged Clone
CAT＃: SC323514
EPHA5 (untagged)-Kinase deficient mutant (K685M) of Human EPH receptor A5 (EPHA5), transcript variant 1
CNY 10,840.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CEK7; EHK-1; EHK1; EK7; HEK7; TYRO4 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_004439, the custom clone sequence may differ by one or more nucleotides
ATGCGGGGCTCGGGGCCCCGGGGTGCGGGACGCCGGCGGCCCCCAAGCGGCGGCGGCGACACCCCCATCA CCCCAGCGTCCCTGGCCGGCTGCTACTCTGCACCTCGACGGGCTCCCCTCTGGACGTGCCTTCTCCTGTG CGCCGCACTCCGGACCCTCCTGGCCAGCCCCAGCAACGAAGTGAATTTATTGGATTCACGCACTGTCATG GGGGACCTGGGATGGATTGCTTTTCCAAAAAATGGGTGGGAAGAGATTGGTGAAGTGGATGAAAATTATG CCCCTATCCACACATACCAAGTATGCAAAGTGATGGAACAGAATCAGAATAACTGGCTTTTGACCAGTTG GATCTCCAATGAAGGTGCTTCCAGAATCTTCATAGAACTCAAATTTACCCTGCGGGACTGCAACAGCCTT CCTGGAGGACTGGGGACCTGTAAGGAAACCTTTAATATGTATTACTTTGAGTCAGATGATCAGAATGGGA GAAACATCAAGGAAAACCAATACATCAAAATTGATACCATTGCTGCCGATGAAAGCTTTACAGAACTTGA TCTTGGTGACCGTGTTATGAAACTGAATACAGAGGTCAGAGATGTAGGACCTCTAAGCAAAAAGGGATTT TATCTTGCTTTTCAAGATGTTGGTGCTTGCATTGCTCTGGTTTCTGTGCGTGTATACTATAAAAAATGCC CTTCTGTGGTACGACACTTGGCTGTCTTCCCTGACACCATCACTGGAGCTGATTCTTCCCAATTGCTCGA AGTGTCAGGCTCCTGTGTCAACCATTCTGTGACCGATGAACCTCCCAAAATGCACTGCAGCGCCGAAGGG GAGTGGCTGGTGCCCATCGGGAAATGCATGTGCAAGGCAGGATATGAAGAGAAAAATGGCACCTGTCAAG TGTGCAGACCTGGGTTCTTCAAAGCCTCACCTCACATCCAGAGCTGCGGCAAATGTCCACCTCACAGTTA TACCCATGAGGAAGCTTCAACCTCTTGTGTCTGTGAAAAGGATTATTTCAGGAGAGAGTCTGATCCACCC ACAATGGCATGCACAAGACCCCCCTCTGCTCCTCGGAATGCCATCTCAAATGTTAATGAAACTAGTGTCT TTCTGGAATGGATTCCGCCTGCTGACACTGGTGGAAGGAAAGACGTGTCATATTATATTGCATGCAAGAA GTGCAACTCCCATGCAGGTGTGTGTGAGGAGTGTGGCGGTCATGTCAGGTACCTTCCCCGGCAAAGCGGC CTGAAAAACACCTCTGTCATGATGGTGGATCTACTCGCTCACACAAACTATACCTTTGAGATTGAGGCAG TGAATGGAGTGTCCGACTTGAGCCCAGGAGCCCGGCAGTATGTGTCTGTAAATGTAACCACAAATCAAGC AGCTCCATCTCCAGTCACCAATGTGAAAAAAGGGAAAATTGCAAAAAACAGCATCTCTTTGTCTTGGCAA GAACCAGATCGTCCCAATGGAATCATCCTAGAGTATGAAATCAAGTATTTTGAAAAGGACCAAGAGACCA GCTACACGATTATCAAATCTAAAGAGACAACTATTACTGCAGAGGGCTTGAAACCAGCTTCAGTTTATGT CTTCCAAATTCGAGCACGTACAGCAGCAGGCTATGGTGTCTTCAGTCGAAGATTTGAGTTTGAAACCACC CCAGTGTTTGCAGCATCCAGCGATCAAAGCCAGATTCCTGTAATTGCTGTGTCTGTGACAGTGGGAGTCA TTTTGTTGGCAGTGGTTATCGGCGTCCTCCTCAGTGGAAGTTGCTGCGAATGTGGCTGTGGGAGGGCTTC TTCCCTGTGCGCTGTTGCCCATCCAAGCCTAATATGGCGGTGTGGCTACAGCAAAGCAAAACAAGATCCA GAAGAGGAAAAGATGCATTTTCATAATGGGCACATTAAACTGCCAGGAGTAAGAACTTACATTGATCCAC ATACCTATGAGGATCCCAATCAAGCTGTCCACGAATTTGCTAAGGAGATAGAAGCATCATGTATCACCAT TGAGAGAGTTATTGGAGCAGGTGAATTTGGTGAAGTTTGTAGTGGACGTTTGAAACTACCAGGAAAAAGA GAATTACCTGTGGCTATCAAAACCCTTAAAGTAGGCTATACTGAAAAGCAACGCAGAGATTTCCTAGGTG AAGCAAGTATCATGGGACAGTTTGATCATCCTAACATCATCCATTTAGAAGGTGTGGTGACCAAAAGTAA ACCAGTGATGATCGTGACAGAGTATATGGAGAATGGCTCTTTAGATACATTTTTGAAGAAAAACGATGGG CAGTTCACTGTGATTCAGCTTGTTGGCATGCTGAGAGGTATCTCTGCAGGAATGAAGTACCTTTCTGACA TGGGCTATGTGCATAGAGATCTTGCTGCCAGAAACATCTTAATCAACAGTAACCTTGTGTGCAAAGTGTC TGACTTTGGACTTTCCCGGGTACTGGAAGATGATCCCGAGGCAGCCTACACCACAAGGGGAGGAAAAATT CCAATCAGATGGACTGCCCCAGAAGCAATAGCTTTCCGAAAGTTTACTTCTGCCAGTGATGTCTGGAGTT ATGGAATAGTAATGTGGGAAGTTGTGTCTTATGGAGAGAGACCCTACTGGGAGATGACCAATCAAGATGT GATTAAAGCGGTAGAGGAAGGCTATCGTCTGCCAAGCCCCATGGATTGTCCTGCTGCTCTCTATCAGTTA ATGCTGGATTGCTGGCAGAAAGAGCGAAATAGCAGGCCCAAGTTTGATGAAATAGTCAACATGTTGGACA AGCTGATACGTAACCCAAGTAGTCTGAAGACGCTGGTTAATGCATCCTGCAGAGTATCTAATTTATTGGC AGAACATAGCCCACTAGGATCTGGGGCCTACAGATCAGTAGGTGAATGGCTAGAGGCAATCAAGATGGGC CGGTATACAGAGATTTTCATGGAAAATGGATACAGTTCAATGGACGCTGTGGCTCAGGTGACCTTGGAGG ATTTGAGACGGCTTGGAGTGACTCTTGTCGGTCACCAGAAGAAGATCATGAACAGCCTTCAAGAAATGAA GGTGCAGCTGGTAAACGGAATGGTGCCATTGTAA >OriGene 5' read for mutant NM_004439 unedited
ACGCCGTCTGAGCAACTGGGCGGTAGGCGCTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTG AACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGAAGCGGTGG ACTGAGCCGCTCGGGACAGCGGCACCGGAGGAGGCTCGGAGAAGATGCGGGGCTCGGGGCCCCGGGGTGC GGGACGCCGGCGGCCCCCAAGCGGCGGCGGCGACACCCCCATCACCCCAGCGTCCCTGGCCGGCTGCTAC TCTGCACCTCGACGGGCTCCCCTCTGGACGTGCCTTCTCCTGTGCGCCGCACTCCGGACCCTCCTGGCCA GCCCCCAGCAACGAAGTGAATTTATTGGATTCACGCACTGTCCATGGGGGGACCTGGGATGGATTGCTTT TTCCAAAAAAGGGGTGGGAAAGGAAATTGGTGGAAGTTGGATGAAAAATTATGCCCCTATCCCCCACTTC CCAATTTTGCAAAGTGGATGGAACAGAACCGAAATACCGGGCTTTGGACCGTTTGGTTCTCCAATGAGGG GCCTCCAGAATCCTTATAGAACTCAAAATTTACCTGGGGGCTGCAAAAGCCCTTCTGGGAGAGACGGGGG GACTGTGTAGGAAACTTTTATATGTATTATCATTTGAGTCTGATAAGAACCAAAGGGAGAAACACTCAGA GAACCCTATATCTCAAATGGTGACCCTTGCTGCTCGCAGAAAACGTTTTAGAGATTGTTCTCGTGGACGC GTGATGAGACTGAGATAGAGTGATCAGATTTGAGCCCTCAGCGAAAAAGGGTTTATCTCGTCTTCAAATG TGGCTTCGCAGGCTCGGGTTCTGGCGTGTTACCTAAAATGCCCTTCTGTGAGCAGCTGGTGGTCTCTGAC CTACTAGACGATATACCATGTGCGGATGCAGTCTCGTGCAACATTGGCAGTAGATCTCTCTATGTCGCCT G >SC323514 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
GARRAGTATTGGAGCAGGTGATTTGGTGAAGTTTGTAGTGGACGTTTGAAACTACCAGGAAAAAGAGAAT TACCTGTGGCTATCATGACCCTTAAAGTAGGCTATACTGAAAAGCAACGCAGAGATTTCCTAGGTGAAGC AAGTATCATGGGACAGTTTGATCATCCTAACATCATCCATTTAGAAGGTGTGGTGACCAAAAGTAAACCA GTGATGATCGTGACAGAGTATATGGAGAATGGCTCTTTAGATACA |
| Restriction Sites | Please inquire |
| ACCN | NM_004439 |
| Insert Size | 5100 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004439.4, NP_004430.3 |
| RefSeq Size | 8418 bp |
| RefSeq ORF | 3114 bp |
| Locus ID | 2044 |
| UniProt ID | P54756 |
| Domains | pkinase, EPH_lbd, TyrKc, SAM, S_TKc, FN3 |
| Protein Families | Druggable Genome, Protein Kinase, Transmembrane |
| Protein Pathways | Axon guidance |
| Gene Summary | This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Aug 2013] Transcript Variant: This variant (1) used an alternate splice site in the coding region compared to variant 3. The resulting protein (isoform a) is shorter but has the same N- and C-termini compared to isoform c. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213206 | EPHA5 (Myc-DDK-tagged)-Human EPH receptor A5 (EPHA5), transcript variant 1 |
CNY 10,832.00 |
|
| RC213206L1 | Lenti-ORF clone of EPHA5 (Myc-DDK-tagged)-Human EPH receptor A5 (EPHA5), transcript variant 1 |
CNY 13,232.00 |
|
| RC213206L2 | Lenti-ORF clone of EPHA5 (mGFP-tagged)-Human EPH receptor A5 (EPHA5), transcript variant 1 |
CNY 7,600.00 |
|
| RC213206L3 | Lenti-ORF clone of EPHA5 (Myc-DDK-tagged)-Human EPH receptor A5 (EPHA5), transcript variant 1 |
CNY 7,600.00 |
|
| RC213206L4 | Lenti-ORF clone of EPHA5 (mGFP-tagged)-Human EPH receptor A5 (EPHA5), transcript variant 1 |
CNY 7,600.00 |
|
| RC600042 | EPHA5 (DDK-His-tagged)-Extra Cellular Domain Clone of Homo sapiens EPH receptor A5, transcript variant 1, Signal peptide (1-24) plus EC domain (25-573) |
CNY 4,264.00 |
|
| RG213206 | EPHA5 (tGFP-tagged) - Human EPH receptor A5 (EPHA5), transcript variant 1 |
CNY 12,432.00 |
|
| SC309998 | EPHA5 (untagged)-Human EPH receptor A5 (EPHA5), transcript variant 1 |
CNY 10,840.00 |
其它Eph receptor A5产品
