Metabotropic Glutamate Receptor 4 (GRM4) (NM_001256811) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	GPRC1D; mGlu4; MGLUR4
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332479 representing NM_001256811. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCCTGGGAAGAGAGGCTTGGGCTGGTGGTGGGCCCGGCTGCCCCTTTGCCTGCTCCTCAGCCTTTAC GGCCCCTGGATGCCTTCCTCCCTGGGAAAGCCCAAAGGCCACCCTCACATGAATTCCATCCGCATAGAT GGGGACATCACACTGGGAGGCCTGTTCCCGGTGCATGGCCGGGGCTCAGAGGGCAAGCCCTGTGGAGAA CTTAAGAAGGAAAAGGGCATCCACCGGCTGGAGGCCATGCTGTTCGCCCTGGATCGCATCAACAACGAC CCGGACCTGCTGCCTAACATCACGCTGGGCGCCCGCATTCTGGACACCTGCTCCAGGGACACCCATGCC CTCGAGCAGTCGCTGACCTTTGTGCAGGCGCTCATCGAGAAGGATGGCACAGAGGTCCGCTGTGGCAGT GGCGGCCCACCCATCATCACCAAGCCTGAACGTGTGGTGGGTGTCATCGGTGCTTCAGGGAGCTCGGTC TCCATCATGGTGGCCAACATCCTTCGCCTCTTCAAGATACCCCAGATCAGCTACGCCTCCACAGCGCCA GACCTGAGTGACAACAGCCGCTACGACTTCTTCTCCCGCGTGGTGCCCTCGGACACGTACCAGGCCCAG GCCATGGTGGACATCGTCCGTGCCCTCAAGTGGAACTATGTGTCCACAGTGGCCTCGGAGGGCAGCTAT GGTGAGAGCGGTGTGGAGGCCTTCATCCAGAAGTCCCGTGAGGACGGGGGCGTGTGCATCGCCCAGTCG GTGAAGATACCACGGGAGCCCAAGGCAGGCGAGTTCGACAAGATCATCCGCCGCCTCCTGGAGACTTCG AACGCCAGGGCAGTCATCATCTTTGCCAACGAGGATGACATCAGGCGTGTGCTGGAGGCAGCACGAAGG GCCAACCAGACAGGCCATTTCTTCTGGATGGGCTCTGACAGCTGGGGCTCCAAGATTGCACCTGTGCTG CACCTGGAGGAGGTGGCTGAGGGTGCTGTCACGATCCTCCCCAAGAGGATGTCCGTACGAGACCGTGAG CGAATTGGGCAGGATTCAGCTTATGAGCAGGAGGGGAAGGTGCAGTTTGTGATCGATGCCGTGTACGCC ATGGGCCACGCGCTGCACGCCATGCACCGTGACCTGTGTCCCGGCCGCGTGGGGCTCTGCCCGCGCATG GACCCTGTAGATGGCACCCAGCTGCTTAAGTACATCCGAAACGTCAACTTCTCAGGCATCGCAGGGAAC CCTGTGACCTTCAATGAGAATGGAGATGCGCCTGGGCGCTATGACATCTACCAATACCAGCTGCGCAAC GATTCTGCCGAGTACAAGGTCATTGGCTCCTGGACTGACCACCTGCACCTTAGAATAGAGCGGATGCAC TGGCCGGGGAGCGGGCAGCAGCTGCCCCGCTCCATCTGCAGCCTGCCCTGCCAACCGGGTGAGCGGAAG AAGACAGTGAAGGGCATGCCTTGCTGCTGGCACTGCGAGCCTTGCACAGGGTACCAGTACCAGGTGGAC CGCTACACCTGTAAGACGTGTCCCTATGACATGCGGCCCACAGAGAACCGCACGGGCTGCCGGCCCATC CCCATCATCAAGCTTGAGTGGGGCTCGCCCTGGGCCGTGCTGCCCCTCTTCCTGGCCGTGGTGGGCATC GCTGCCACGTTGTTCGTGGTGATCACCTTTGTGCGCTACAACGACACGCCCATCGTCAAGGCCTCGGGC CGTGAACTGAGCTACGTGCTGCTGGCAGGCATCTTCCTGTGCTATGCCACCACCTTCCTCATGATCGCT GAGCCCGACCTTGGCACCTGCTCGCTGCGCCGAATCTTCCTGGGACTAGGGATGAGCATCAGCTATGCA GCCCTGCTCACCAAGACCAACCGCATCTACCGCATCTTCGAGCAGGGCAAGCGCTCGGTCAGTGCCCCA CGCTTCATCAGCCCCGCCTCACAGCTGGCCATCACCTTCAGCCTCATCTCGCTGCAGCTGCTGGGCATC TGTGTGTGGTTTGTGGTGGACCCCTCCCACTCGGTGGTGGACTTCCAGGACCAGCGGACACTCGACCCC CGCTTCGCCAGGGGTGTGCTCAAGTGTGACATCTCGGACCTGTCGCTCATCTGCCTGCTGGGCTACAGC ATGCTGCTCATGGTCACGTGCACCGTGTATGCCATCAAGACACGCGGCGTGCCCGAGACCTTCAATGAG GCCAAGCCCATTGGCTTCACCATGTACACCACTTGCATCGTCTGGCTGGCCTTCATCCCCATCTTCTTT GGCACCTCGCAGTCGGCCGACAAGCTGTACATCCAGACGACGACGCTGACGGTCTCGGTGAGTCTGAGC GCCTCGGTGTCCCTGGGAATGCTCTACATGCCCAAAGTCTACATCATCCTCTTCCACCCGGAGCAGAAC GTGCCCAAGCGCAAGCGCAGCCTCAAAGCCGTCGTTACGGCGGCCACCATGTCCAACAAGTTCACGCAG AAGGGCAACTTCCGGCCCAACGGAGAGGCCAAGTCTGAGCTCTGCGAGAACCTTGAGGCCCCAGCGCTG GCCACCAAACAGACTTACGTCACTTACACCAACCATGCAATCTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001256811
Insert Size	2598 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001256811.2
RefSeq Size	7293 bp
RefSeq ORF	2598 bp
Locus ID	2914
UniProt ID	Q14833
Protein Families	Druggable Genome, GPCR, Transmembrane
Protein Pathways	Neuroactive ligand-receptor interaction, Taste transduction
MW	96.1 kDa
Gene Summary	L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012] Transcript Variant: This variant (4) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (4) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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