BRD8 (NM_001300966) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	p120; SMAP; SMAP2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001300966, the custom clone sequence may differ by one or more nucleotides ATGAGCTTTGCAATGACATTGAAAAAGAAATTGGAAGAAGAGGAGGCTGAAGTAAAGAGGAAGGCTACAG ATGCTGCATACCAGGCTCGTCAAGCAGTAAAAACACCCCCCCGGAGGTTACCCACTGTGATGGTTCGCTC TCCTATAGATTCTGCCTCCCCAGGAGGTGATTATCCACTTGGGGACTTGACTCCAACCACTATGGAAGAG GCTACCTCTGGGGTAACCCCCGGGACTTTGCCGAGTACCCCAGTCACCTCGTTTCCTGGGATTCCTGACA CCCTTCCTCCAGGCTCTGCACCCTTAGAAGCCCCCATGACCCCAGTAACAGATGATTCACCCCAGAAAAA GATGCTTGGACAGAAAGCAACTCCACCCCCCTCCCCTCTGCTGTCAGAGCTCTTGAAGAAGGGCAGCCTC CTGCCTACTAGCCCCAGACTGGTCAATGAGAGTGAAATGGCTGTGGCTTCTGGCCACCTGAACAGTACAG GTGTCCTCCTGGAGGTAGGCGGGGTCCTTCCCATGATACATGGTGGGGAGATACAGCAAACACCCAATAC TGTTGCAGCCTCCCCTGCTGCATCAGAGTCTGTGTCCCAAGCTACCATTGTCATGATGCCTGCGCTGCCA GCACCATCCTCTGCTCCGGCTGTCTCCACTACTGAAAGTGTAGCTCCAGTGAGTCAACCCGACAACTGTG TTCCCATGGAGGCTGTGGGGGATCCACATACTGTGACTGTTTCCATGGACAGCAGTGAAATATCCATGAT CATCAATTCTATCAAAGAAGAGTGTTTTCGATCAGGGGTAGCAGAGGCTCCTGTTGGATCAAAGGCTCCC AGCATAGATGGGAAGGAAGAATTAGATCTGGCTGAGAAGATGGATATTGCTGTGTCTTACACAGGTGAAG AGCTGGATTTTGAGACTGTTGGAGACATCATTGCCATCATTGAGGACAAGGTAGATGATCATCCTGAAGT GCTGGATGTGGCAGCAGTGGAAGCAGCACTGTCATTTTGTGAAGAAAATGATGATCCTCAGTCCCTGCCT GGCCCCTGGGAGCATCCTATCCAGCAGGAGCGGGACAAGCCAGTACCTCTCCCTGCACCAGAAATGACGG TCAAGCAAGAGAGACTGGACTTTGAGGAAACGGAAAACAAGGGAATACATGAACTGGTGGACATCAGGGA GCCCAGTGCAGAGATCAAGGTGGAACCTGCAGAACCAGAGCCAGTCATTTCAGGAGCCGAAATAGTAGCT GGAGTTGTTCCAGCCACAAGTATGGAGCCACCAGAACTCAGGAGTCAGGACTTAGATGAGGAACTGGGAA GTACTGCAGCTGGAGAGATTGTTGAAGCAGATGTTGCCATTGGGAAAGGCGATGAGACTCCACTTACAAA TGTGAAGACAGAGGCATCCCCTGAAAGCATGTTGTCTCCATCACATGGCTCAAATCCCATTGAAGATCCT TTAGAGGCAGAGACTCAGCACAAGTTTGAAATGTCAGACTCATTGAAAGAAGAATCAGGGACTATTTTTG GAAGCCAGATAAAGGATGCCCCAGGTGAGGATGAGGAGGAAGATGGTGTCAGTGAAGCGGCCAGCCTAGA GGAGCCTAAGGAAGAGGATCAAGGAGAAGGCTACTTGTCAGAAATGGATAATGAACCTCCTGTGAGCGAG AGTGATGATGGCTTCAGCATACACAATGCTACACTGCAGTCACACACACTGGCAGACTCCATCCCCAGCA GCCCTGCTTCTTCACAGTTCTCTGTCTGTAGTGAGGATCAGGAAGCTATTCAGGCACAGAAAATTTGGAA GAAAGCCATCATGCTTGTATGGAGAGCTGCAGCTAATCATAGGTATGCCAATGTCTTCCTGCAGCCTGTT ACAGATGACATAGCACCTGGCTACCACAGCATTGTGCAGAGGCCTATGGATTTGTCAACTATTAAGAAAA ACATAGAAAATGGACTGATCCGAAGCACAGCTGAATTTCAGCGTGACATTATGCTGATGTTTCAGAATGC TGTAATGTACAATAGCTCAGACCATGATGTCTATCACATGGCAGTGGAGATGCAGCGAGATGTCTTGGAA CAGATCCAGCAATTCTTGGCCACGCAGTTGATTATGCAAACATCCGAGTCTGGGATCAGTGCTAAAAGTC TTCGAGGGAGAGATTCTACCCGCAAACAGGATGCTTCAGAGAAGGATGGAGGAACCAGGGGACGCCGCTG TGCCATTGAAGCAGATATGAAGATGAAAAAGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001300966
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001300966.1, NP_001287895.1
RefSeq Size	3086 bp
RefSeq ORF	2274 bp
Locus ID	10902
UniProt ID	Q9H0E9
Protein Families	Druggable Genome, Transcription Factors
Gene Summary	The protein encoded by this gene interacts with thyroid hormone receptor in a ligand-dependent manner and enhances thyroid hormone-dependent activation from thyroid response elements. This protein contains a bromodomain and is thought to be a nuclear receptor coactivator. Multiple alternatively spliced transcript variants that encode distinct isoforms have been identified. [provided by RefSeq, Jul 2014] Transcript Variant: This variant (7) has an alternate splice site in the 5' region, which results in an alternate translation start codon, and has an alternate splice site in the central region and also lacks an in-frame exon in the 3' region, compared to variant 1. The resulting isoform (7) has a shorter and distinct N-terminus and lacks two internal segments, compared to isoform 1.

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