K15 (NC_009333) Virus Tagged ORF Clone

CAT＃: VC101715

TrueORF^®

Myc-DDK-tagged ORF clone of viral ORF for K15 [Human herpesvirus 8], codon optimized for human cell expression, YP_001129435

Datasheet

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CNY 5,990.00

货期*

5周

规格

Product images

经常一起买 (4)

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Specifications

Product Data
Type	Virus Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >The Viral ORF clone VC101715 represents NCBI reference of YP_001129435 with codon optimized for human cell expression Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGACTCTTATCTTCTTTTGGAATCTTTGGCTTTGGGCTCTTTTGGTATGTTTCTGGTGCATCACCC TGGTGTGCGTTACTACTAATAGTATTGACACAATGGCCTCCTTGTTGGTAATGTGTATCCTCTTCGTTTC AGCCATCAACAAGTATACCCAAGCGATATCAAGCAATAATCCTAAGTGGCCTAGCTCCTGGCACCTCGGT ATTATTGCCTGCATAGTACTGAAGCTTTGGAACCTGAGCACAACTAATTCCGTGACCTATGCATGCCTGA TTACCACAGCAATCCTCAGCCTTGTGACCGCTTTCCTCACCCTGATAAAGCACTGCACGGCCTGCAAACT GCAGCTTGAGCACGGTATTCTTTTCACTTCCACATTTGCAGTACTGATGACCAATATGTTGGTTCACATG TCCAATACCTGGCAGTCCTCCTGGATTTTCTTTCCCATCTCATTTACCCTGTCTCTGCCTTTCCTGTACG CTTTCGCCACTGTGAAGACAGGCAATATAAAGTTGGTGAGCTCCGTGAGCTTCATCTGTGCCGGTCTCGT AATGGGTTACCCTGTATCTTGCTGTAAAACACACACGTGCACCGCGACCGCAGCCGGCCTTTCCCTCTCA TCTATATATCTCGGCTTTACCGGAATTATAAGTACCCTCCACAAAAGCTGGGCTCCTCCAAAGCGGGGTA TTCTCACATTTCTTCTGTTGCAGGGGGGGGTGTTGACAACACAGACCCTTACAACTGAACTCCTGGCTAT CACCTCAACCACTGGGAACATCAAGGGGCACGAAATCCTGCTCCTCGTGTGTCTCATTTTCCTCTGGTGC CTTTATGTTTGGCAGTCCTTCAACAAGGCTTCTCTCGTCACCGGCATGCTGCACCTGATTGCAGCCTGGT CACACACAGGTGGATGCGTTCAACTCGTTATGCTTCTGCCTTCAGGACTTACTAGGGGTATCTTGACTAT GATAATTTGTATATCAACGCTCTTCAGTACGCTCCAGGGACTTTTGGTGTTCTACCTGTATAAAGAAAAG AAAGTGGTCGCTGTGAACAGCTACCGGCAGCGCAGACGGCGGATTTATACTAGAGACCAAAACCTGCATC ACAACGACAATCATCTGGGAAACAATGTGATCAGTCCTCCTCCCCTTCCTCCCTTTTTCCGGCAGCCCGT TCGACTTCCCAGTCACGTGACGGACCGCGGGAGAGGCTCCCAACTGCTTAACGAGGTGGAGCTCCAGGAG GTGAACCGGGACCCCCCGAACGTGTTTGGCTACGCCAGCATCCTGGTGTCAGGAGCCGAGGAAAGCCGCG AACCGAGTCCCCAACCCGATCAGTCAGGGATGTCAATTCTGAGGGTGGATGGGGGTAGCGCGTTCCGGAT CGATACCGCACAGGCCGCTACGCAACCAACCGATGACCTCTACGAGGAAGTGCTGTTCCCAAGAAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >VC101715 representing YP_001129435 Red=Cloning sites Green=Tags MKTLIFFWNLWLWALLVCFWCITLVCVTTNSIDTMASLLVMCILFVSAINKYTQAISSNNPKWPSSWHLG IIACIVLKLWNLSTTNSVTYACLITTAILSLVTAFLTLIKHCTACKLQLEHGILFTSTFAVLMTNMLVHM SNTWQSSWIFFPISFTLSLPFLYAFATVKTGNIKLVSSVSFICAGLVMGYPVSCCKTHTCTATAAGLSLS SIYLGFTGIISTLHKSWAPPKRGILTFLLLQGGVLTTQTLTTELLAITSTTGNIKGHEILLLVCLIFLWC LYVWQSFNKASLVTGMLHLIAAWSHTGGCVQLVMLLPSGLTRGILTMIICISTLFSTLQGLLVFYLYKEK KVVAVNSYRQRRRRIYTRDQNLHHNDNHLGNNVISPPPLPPFFRQPVRLPSHVTDRGRGSQLLNEVELQE VNRDPPNVFGYASILVSGAEESREPSPQPDQSGMSILRVDGGSAFRIDTAQAATQPTDDLYEEVLFPRN TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI Cloning Scheme for this gene
ACCN	NC_009333
ORF Size	1467 bp
OTI Disclaimer	The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NC_009333.1, YP_001129435
RefSeq ORF	1467 bp
Locus ID	4961473
UniProt ID	Q2HR82
MW	54.3 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

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VC101632	Myc-DDK-tagged ORF clone of viral ORF for ORF6 [Human herpesvirus 8], codon optimized for human cell expression, YP_001129352	10 ug 产品选项验证失败所有选项都是必需的	CNY 17,100.00
VC101633	Myc-DDK-tagged ORF clone of viral ORF for ORF7 [Human herpesvirus 8], codon optimized for human cell expression, YP_001129353	10 ug 产品选项验证失败所有选项都是必需的	CNY 10,450.00
VC101634	Myc-DDK-tagged ORF clone of viral ORF for ORF8 [Human herpesvirus 8], codon optimized for human cell expression, YP_001129354	10 ug 产品选项验证失败所有选项都是必需的	CNY 12,830.00
VC101635	Myc-DDK-tagged ORF clone of viral ORF for ORF9 [Human herpesvirus 8], codon optimized for human cell expression, YP_001129355	10 ug 产品选项验证失败所有选项都是必需的	CNY 15,390.00
VC101636	Myc-DDK-tagged ORF clone of viral ORF for ORF10 [Human herpesvirus 8], codon optimized for human cell expression, YP_001129356	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,130.00
VC101637	Myc-DDK-tagged ORF clone of viral ORF for ORF11 [Human herpesvirus 8], codon optimized for human cell expression, YP_001129357	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,940.00
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VC101644	Myc-DDK-tagged ORF clone of viral ORF for K42 [Human herpesvirus 8], codon optimized for human cell expression, YP_001129364	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
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VC101655	Myc-DDK-tagged ORF clone of viral ORF for ORF22 [Human herpesvirus 8], codon optimized for human cell expression, YP_001129375	10 ug 产品选项验证失败所有选项都是必需的	CNY 11,020.00
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VC101708	Myc-DDK-tagged ORF clone of viral ORF for K12 [Human herpesvirus 8], codon optimized for human cell expression, YP_001129428	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC101709	Myc-DDK-tagged ORF clone of viral ORF for K13 [Human herpesvirus 8], codon optimized for human cell expression, YP_001129429	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC101710	Myc-DDK-tagged ORF clone of viral ORF for ORF72 [Human herpesvirus 8], codon optimized for human cell expression, YP_001129430	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC101712	Myc-DDK-tagged ORF clone of viral ORF for K14 [Human herpesvirus 8], codon optimized for human cell expression, YP_001129432	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC101713	Myc-DDK-tagged ORF clone of viral ORF for ORF74 [Human herpesvirus 8], codon optimized for human cell expression, YP_001129433	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,488.00
VC101714	Myc-DDK-tagged ORF clone of viral ORF for ORF75 [Human herpesvirus 8], codon optimized for human cell expression, YP_001129434	10 ug 产品选项验证失败所有选项都是必需的	CNY 19,570.00

其它K15产品

Expression Plasmids

Lentiviral Particles

CRISPR

RNAi

Proteins

Antibodies