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Srf (NM_020493) Mouse Tagged ORF Clone
CAT＃: MR208120
- TrueORF®
Srf (Myc-DDK-tagged) - Mouse serum response factor (Srf)
ORF Plasmid: tGFP
"NM_020493" in other vectors (6)
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CNY 5,632.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AW049942; AW240594 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208120 representing NM_020493
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTACCGAGCCAAGCTGGGGCCGCGGCGGCTCTGGGCCGGGGCTCGGCCCTGGGGGGCAACCTGAACC GGACCCCGACGGGGCGGCCGGGCGGCGGCGGCGGGACTCGCGGGGCGAACGGGGGCCGGGTCCCCGGGAA CGGCGCGGGGCTCGGCCAGAGTCGTCTGGAGCGGGAGGCTGCAGCGGCAGCGGCGCCCACCGCCGGGGCC CTCTACAGCGGCAGCGAGGGCGACTCCGAGTCCGGCGAGGAGGAGGAGCTGGGCGCCGAGCGGCGCGGCC TCAAGCGGAGCCTGAGCGAGATGGAGCTCGGCGTGGTGGTCGGTGGGCCTGAGGCGGCGGCGGCGGCCGC CGGGGGCTACGGGCCGGTGAGCGGCGCGGTGAGCGGGGCCAAGCCGGGGAAGAAGACCCGGGGCCGCGTG AAGATCAAGATGGAGTTCATCGACAACAAGCTGCGGCGCTACACGACCTTCAGCAAGAGGAAGACGGGCA TCATGAAGAAGGCCTATGAGCTGTCCACGCTGACAGGGACACAGGTGCTGTTGCTGGTGGCCAGTGAGAC AGGCCATGTGTATACCTTTGCCACCCGCAAACTGCAGCCCATGATCACCAGTGAGACCGGCAAGGCGCTG ATTCAGACCTGCCTCAACTCGCCAGACTCTCCGCCCCGCTCAGACCCCACCACAGACCAGAGAATGAGTG CCACTGGCTTTGAAGAGCCAGATCTCACCTACCAGGTGTCGGAATCTGACAGCAGTGGGGAAACCAAGGA CACACTGAAGCCAGCATTCACAGTCACCAACCTGCCGGGTACCACCTCCACAATCCAGACAGCACCCAGC ACCTCTACCACCATGCAAGTCAGCAGCGGCCCCTCCTTCCCCATCACCAACTACCTGGCACCAGTGTCTG CTAGTGTCAGCCCCAGCGCTGTCAGCAGTGCCAACGGGACTGTGCTCAAGAGTACAGGCAGCGGCCCTGT CTCCTCTGGGGGCCTTATGCAGCTGCCTACCAGCTTCACCCTCATGCCTGGTGGGGCAGTGGCCCAGCAG GTCCCTGTGCAGGCCATTCATGTGCACCAGGCCCCACAGCAAGCGTCTCCCTCTCGTGACAGCAGCACAG ACCTCACGCAGACCTCCTCCAGCGGGACAGTGACGTTGCCCGCCACCATCATGACGTCGTCTGTACCCAC AACTGTGGGTGGCCACATGATGTACCCTAGTCCCCATGCAGTGATGTATGCCCCCACCTCAGGCCTGGCT GATGGCAGCCTCACCGTGCTCAATGCCTTCTCTCAGGCACCATCCACCATGCAGGTGTCCCACAGCCAGG TCCAGGAGCCAGGTGGTGTCCCTCAGGTGTTCCTGACAGCACCGTCTGGGACCGTGCAGATCCCTGTCTC TGCAGTTCAGCTTCACCAGATGGCTGTGATAGGGCAGCAAGCTGGGAGCAGCAGCAACCTCACCGAGCTA CAGGTGGTGAACCTGGATGCCACCCACAGCACCAAGAGTGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208120 representing NM_020493
Red=Cloning site Green=Tags(s) MLPSQAGAAAALGRGSALGGNLNRTPTGRPGGGGGTRGANGGRVPGNGAGLGQSRLEREAAAAAAPTAGA LYSGSEGDSESGEEEELGAERRGLKRSLSEMELGVVVGGPEAAAAAAGGYGPVSGAVSGAKPGKKTRGRV KIKMEFIDNKLRRYTTFSKRKTGIMKKAYELSTLTGTQVLLLVASETGHVYTFATRKLQPMITSETGKAL IQTCLNSPDSPPRSDPTTDQRMSATGFEEPDLTYQVSESDSSGETKDTLKPAFTVTNLPGTTSTIQTAPS TSTTMQVSSGPSFPITNYLAPVSASVSPSAVSSANGTVLKSTGSGPVSSGGLMQLPTSFTLMPGGAVAQQ VPVQAIHVHQAPQQASPSRDSSTDLTQTSSSGTVTLPATIMTSSVPTTVGGHMMYPSPHAVMYAPTSGLA DGSLTVLNAFSQAPSTMQVSHSQVQEPGGVPQVFLTAPSGTVQIPVSAVQLHQMAVIGQQAGSSSNLTEL QVVNLDATHSTKSE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020493 |
| ORF Size | 1512 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020493.2, NP_065239.1 |
| RefSeq Size | 4093 bp |
| RefSeq ORF | 1515 bp |
| Locus ID | 20807 |
| UniProt ID | Q9JM73 |
| MW | 51.7 kDa |
| Gene Summary | SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS) (PubMed:24732378). Together with MRTFA transcription coactivator, controls expression of genes regulating the cytoskeleton during development, morphogenesis and cell migration (PubMed:12732141, PubMed:19350017, PubMed:24732378). The SRF-MRTFA complex activity responds to Rho GTPase-induced changes in cellular globular actin (G-actin) concentration, thereby coupling cytoskeletal gene expression to cytoskeletal dynamics (PubMed:24732378). Required for cardiac differentiation and maturation (PubMed:15169892).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217127 | Srf (untagged) - Mouse serum response factor (Srf), (10ug) |
CNY 3,760.00 |
|
| MG208120 | Srf (tGFP-tagged) - Mouse serum response factor (Srf) |
CNY 7,232.00 |
|
| MR208120L1 | Lenti ORF clone of Srf (Myc-DDK-tagged) - Mouse serum response factor (Srf) |
CNY 6,940.00 |
|
| MR208120L2 | Lenti ORF clone of Srf (mGFP-tagged) - Mouse serum response factor (Srf) |
CNY 8,032.00 |
|
| MR208120L3 | Lenti ORF clone of Srf (Myc-DDK-tagged) - Mouse serum response factor (Srf) |
CNY 6,940.00 |
|
| MR208120L4 | Lenti ORF clone of Srf (mGFP-tagged) - Mouse serum response factor (Srf) |
CNY 8,032.00 |
