United States
Germany
Japan
United Kingdom
China
C5ar1 (NM_007577) Mouse Tagged ORF Clone
CAT＃: MR225064
- TrueORF®
C5ar1 (Myc-DDK-tagged) - Mouse complement component 5a receptor 1 (C5ar1), transcript variant 1
ORF Plasmid: tGFP
"NM_007577" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 5,488.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | C5aR; C5r1; Cd88; D7Msu1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR225064 representing NM_007577
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCCCATAGATAACAGCAGCTTTGAAATCAACTATGATCACTATGGAACCATGGATCCTAACATAC CTGCGGATGGCATTCACCTCCCGAAGCGGCAACCTGGGGATGTTGCAGCCCTTATCATCTACTCGGTGGT GTTCCTGGTGGGAGTACCCGGGAATGCCCTGGTGGTGTGGGTGACAGCCTTCGAGGCCAGACGGGCCGTC AACGCCATCTGGTTTCTGAATCTGGCGGTGGCCGACCTCCTCTCGTGCTTGGCACTGCCTGTCCTGTTCA CGACCGTTTTAAATCATAACTACTGGTACTTTGATGCCACCGCCTGTATAGTCCTGCCCTCGCTCATCCT GCTCAACATGTACGCCAGTATCCTGCTGCTGGCTACCATTAGTGCCGACCGTTTCCTGCTGGTGTTCAAG CCCATCTGGTGTCAGAAGGTCCGCGGGACTGGCCTGGCATGGATGGCCTGTGGAGTGGCCTGGGTCTTAG CATTGCTCCTCACCATTCCATCCTTCGTGTACCGGGAGGCATATAAGGACTTCTACTCAGAGCACACTGT ATGTGGTATTAACTATGGTGGGGGTAGCTTCCCCAAAGAGAAGGCTGTGGCCATCCTGCGGCTGATGGTG GGTTTTGTGTTGCCTCTGCTCACTCTAAACATCTGCTACACCTTCCTCCTGCTCCGGACCTGGAGTCGCA AGGCCACGCGCTCCACCAAGACGCTCAAAGTGGTGATGGCTGTGGTCATCTGTTTCTTTATCTTCTGGCT GCCCTATCAGGTGACCGGGGTGATGATAGCGTGGCTGCCCCCGTCCTCGCCCACCTTGAAGAGGGTGGAG AAGCTGAACTCCCTGTGCGTGTCCCTGGCCTACATCAACTGCTGTGTTAACCCTATCATCTACGTCATGG CTGGCCAGGGTTTCCATGGACGACTCCTAAGGTCTCTCCCCAGCATCATACGAAACGCTCTCTCTGAGGA TTCAGTGGGCAGGGATAGCAAGACTTTCACTCCGTCCACGACGGACACCTCAACCCGGAAGAGTCAGGCG GTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225064 representing NM_007577
Red=Cloning site Green=Tags(s) MDPIDNSSFEINYDHYGTMDPNIPADGIHLPKRQPGDVAALIIYSVVFLVGVPGNALVVWVTAFEARRAV NAIWFLNLAVADLLSCLALPVLFTTVLNHNYWYFDATACIVLPSLILLNMYASILLLATISADRFLLVFK PIWCQKVRGTGLAWMACGVAWVLALLLTIPSFVYREAYKDFYSEHTVCGINYGGGSFPKEKAVAILRLMV GFVLPLLTLNICYTFLLLRTWSRKATRSTKTLKVVMAVVICFFIFWLPYQVTGVMIAWLPPSSPTLKRVE KLNSLCVSLAYINCCVNPIIYVMAGQGFHGRLLRSLPSIIRNALSEDSVGRDSKTFTPSTTDTSTRKSQA V TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007577 |
| ORF Size | 1053 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007577.1, NM_007577.2, NM_007577.3, NM_007577.4, NP_031603.2 |
| RefSeq Size | 2481 bp |
| RefSeq ORF | 1056 bp |
| Locus ID | 12273 |
| UniProt ID | P30993 |
| MW | 39.5 kDa |
| Gene Summary | Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. The ligand interacts with at least two sites on the receptor: a high-affinity site on the extracellular N-terminus, and a second site in the transmembrane region which activates downstream signaling events. Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release and superoxide anion production.[UniProtKB/Swiss-Prot Function] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
MK801 regulates the expression of key osteoarthritis factors in osteoarthritis synovial fibroblasts through complement C5
,Huang, Z;Feng, Y;Zhu, X;Wang, L;Lu, W;,
Research in Veterinary Science
,PubMed ID 33799167
[C5AR1]
|
|
Primary squamous cell carcinoma of the extrahepatic bile duct: A case report
,Delgado Maroto, A;Barrientos Delgado, A;Lázaro Sáez, M;Hallouch Toutouh, S;Práxedes González, E;,
Gastroenterol Hepatol
,PubMed ID 31126636
[C5AR1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC208206 | C5ar1 (untagged) - Mouse complement component 5a receptor 1 (C5ar1), transcript variant 1, (10ug) |
CNY 5,488.00 |
|
| MG225064 | C5ar1 (tGFP-tagged) - Mouse complement component 5a receptor 1 (C5ar1) transcript variant 1, (10ug) |
CNY 7,088.00 |
|
| MR225064L3 | Lenti ORF clone of C5ar1 (Myc-DDK-tagged) - Mouse complement component 5a receptor 1 (C5ar1), transcript variant 1 |
CNY 5,230.00 |
|
| MR225064L4 | Lenti ORF clone of C5ar1 (mGFP-tagged) - Mouse complement component 5a receptor 1 (C5ar1), transcript variant 1 |
CNY 7,888.00 |
