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PAPP A (PAPPA) (NM_002581) Human Tagged ORF Clone
CAT＃: RC209767
- TrueORF®
PAPPA (Myc-DDK-tagged)-Human pregnancy-associated plasma protein A, pappalysin 1 (PAPPA)
ORF Plasmid: tGFP
"NM_002581" in other vectors (6)
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CNY 16,696.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ASBABP2; DIPLA1; IGFBP-4ase; PAPA; PAPP-A; PAPPA1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC209767 representing NM_002581
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGCTCTGGAGTTGGGTGCTGCACCTGGGGCTGCTGAGCGCCGCGCTGGGCTGCGGGCTGGCCGAGC GTCCCCGCCGGGCCCGGAGAGACCCGCGGGCCGGCCGACCCCCGCGCCCCGCCGCCGGCCCGGCCACCTG CGCCACCCGGGCGGCCCGCGGCCGCCGCGCCTCGCCGCCGCCGCCGCCGCCGCCGGGCGGTGCCTGGGAA GCCGTGCGCGTCCCCCGGCGGCGGCAGCAGCGGGAGGCGAGGGGCGCCACCGAGGAGCCGAGCCCGCCGA GCCGGGCGCTCTATTTCAGCGGGCGAGGCGAGCAGCTGCGCCTCCGGGCCGACCTCGAGCTGCCCCGGGA CGCGTTCACGCTGCAAGTGTGGCTGCGAGCGGAGGGGGGCCAGAGGTCTCCGGCAGTGATCACAGGGCTG TATGACAAATGTTCTTATATCTCACGTGACCGAGGATGGGTCGTGGGCATTCACACCATCAGTGACCAAG ACAACAAAGACCCACGCTACTTTTTCTCCTTGAAGACAGACCGAGCCCGGCAAGTGACCACCATCAATGC CCACCGCAGCTACCTCCCAGGCCAGTGGGTATACCTAGCTGCCACCTATGATGGGCAGTTCATGAAGCTC TATGTGAATGGTGCCCAGGTGGCCACCTCTGGGGAACAAGTGGGTGGCATATTCAGCCCACTGACCCAGA AGTGCAAAGTGCTCATGTTAGGGGGCAGTGCCCTGAATCACAACTACCGGGGCTACATCGAGCACTTCAG TCTGTGGAAGGTGGCCAGGACTCAGCGGGAGATACTGTCTGACATGGAAACCCATGGCGCCCACACTGCT CTACCTCAGCTCCTCCTCCAGGAGAACTGGGACAATGTGAAGCATGCCTGGTCCCCCATGAAGGATGGCA GCAGCCCCAAAGTGGAATTCAGCAATGCCCACGGCTTTCTGCTGGACACGAGTCTGGAGCCTCCTCTGTG CGGACAGACATTGTGTGACAACACAGAGGTCATTGCCAGCTACAATCAGCTCTCAAGTTTCCGCCAGCCC AAGGTGGTGCGCTACCGCGTGGTCAACCTCTATGAAGATGATCATAAGAACCCGACGGTGACGCGCGAGC AGGTGGACTTCCAGCACCATCAGCTGGCTGAGGCCTTCAAGCAATACAACATCTCCTGGGAGCTGGACGT GCTGGAGGTGAGCAACTCCTCCCTTCGCCGCCGCCTCATCCTGGCCAACTGTGACATCAGCAAGATTGGG GATGAGAACTGTGACCCCGAGTGCAACCACACGCTGACGGGCCACGACGGCGGGGATTGCCGCCACCTGC GCCACCCTGCCTTCGTGAAGAAGCAGCACAACGGGGTGTGTGACATGGACTGCAACTATGAACGGTTCAA CTTTGATGGTGGAGAGTGCTGTGACCCTGAAATCACCAATGTCACTCAGACTTGCTTTGACCCCGACTCT CCACACAGAGCCTACTTGGATGTTAATGAGCTGAAGAACATTCTTAAATTGGATGGATCAACACATCTCA ATATTTTCTTTGCAAAATCCTCAGAGGAGGAGTTGGCAGGAGTAGCAACTTGGCCATGGGACAAGGAGGC CCTGATGCACTTAGGTGGCATTGTCTTGAACCCATCTTTCTATGGCATGCCTGGGCACACCCACACCATG ATCCATGAGATTGGTCACAGCCTGGGCCTCTATCACGTCTTCCGAGGCATCTCAGAAATCCAGTCCTGCA GTGACCCCTGCATGGAGACAGAGCCCTCCTTCGAGACTGGAGACCTCTGCAATGATACCAACCCAGCCCC TAAACACAAGTCCTGTGGTGACCCAGGGCCAGGAAATGACACCTGTGGCTTTCATAGCTTCTTCAACACT CCTTACAACAACTTCATGAGCTATGCAGATGACGACTGTACGGACTCCTTCACGCCCAATCAAGTCGCCA GAATGCACTGTTACCTGGACCTGGTCTACCAGGGCTGGCAGCCCTCCAGGAAACCAGCGCCTGTTGCCCT CGCCCCCCAAGTTCTGGGCCACACAACGGACTCTGTGACACTGGAGTGGTTCCCACCTATAGATGGCCAT TTCTTTGAAAGAGAATTGGGATCAGCATGTCATCTTTGCCTGGAAGGGAGAATCCTGGTGCAGTATGCTT CCAACGCTTCCTCCCCAATGCCCTGCAGCCCATCAGGACACTGGAGCCCTCGTGAAGCAGAAGGTCATCC TGATGTTGAACAGCCCTGTAAGTCCAGTGTCCGCACCTGGAGCCCAAATTCAGCTGTCAACCCACACACG GTTCCTCCAGCCTGCCCTGAGCCTCAAGGCTGCTACCTCGAGCTGGAGTTCCTCTACCCCTTGGTCCCTG AGTCTCTGACCATTTGGGTGACCTTTGTCTCCACTGACTGGGACTCTAGTGGAGCTGTCAATGACATCAA ACTGTTGGCTGTCAGTGGGAAGAACATCTCCCTGGGTCCTCAGAATGTCTTCTGTGATGTCCCACTGACC ATCAGACTCTGGGACGTGGGCGAGGAGGTGTATGGCATCCAAATCTACACGCTGGATGAGCACCTGGAGA TCGATGCTGCCATGTTGACCTCCACTGCAGACACCCCACTCTGTCTACAGTGTAAGCCCCTGAAGTATAA GGTGGTCCGGGACCCTCCTCTCCAGATGGATGTGGCCTCCATCCTACATCTCAATAGGAAATTCGTAGAC ATGGATCTAAATCTTGGCAGTGTGTACCAGTATTGGGTCATAACTATTTCAGGAACTGAAGAGAGTGAGC CATCACCTGCTGTCACATACATCCATGGAAGTGGGTACTGTGGCGATGGCATTATACAAAAAGACCAAGG TGAACAATGCGACGACATGAATAAGATCAATGGTGATGGCTGCTCCCTTTTCTGCCGACAAGAAGTCTCC TTCAATTGTATTGATGAACCCAGCCGGTGCTATTTCCATGATGGTGATGGGGTATGTGAGGAGTTTGAAC AAAAAACCAGCATTAAGGACTGTGGTGTCTACACGCCCCAGGGATTCCTGGATCAGTGGGCATCCAATGC TTCAGTATCTCATCAAGACCAGCAATGCCCAGGCTGGGTCATCATCGGACAGCCAGCAGCATCCCAGGTG TGTCGAACCAAGGTGATAGATCTCAGTGAAGGCATTTCCCAGCATGCCTGGTACCCTTGCACCATCAGCT ACCCATATTCCCAGCTGGCTCAGACCACTTTTTGGCTCCGGGCGTATTTTTCTCAACCAATGGTTGCCGC AGCTGTCATTGTCCACCTGGTGACGGATGGGACATATTATGGGGACCAAAAGCAGGAGACCATCAGCGTG CAGCTGCTTGATACCAAAGATCAGAGCCACGATCTAGGCCTCCATGTCCTGAGCTGCAGGAACAATCCCC TGATTATCCCTGTGGTCCATGACCTCAGCCAGCCCTTCTACCACAGCCAGGCGGTACGTGTGAGCTTCAG TTCGCCCCTGGTCGCCATCTCGGGGGTGGCCCTCCGTTCCTTCGACAACTTTGACCCCGTCACCCTGAGC AGCTGCCAGAGAGGGGAGACCTACAGCCCTGCCGAGCAGAGCTGCGTGCACTTCGCATGTGAGAAAACTG ACTGTCCAGAGCTGGCTGTGGAGAATGCTTCTCTCAATTGCTCCAGCAGCGACCGCTACCACGGTGCCCA GTGTACTGTGAGCTGCCGGACAGGCTACGTGCTCCAGATACGGCGGGATGATGAGCTGATCAAGAGCCAG ACGGGACCCAGCGTCACAGTGACCTGTACAGAGGGCAAGTGGAATAAGCAGGTGGCCTGTGAGCCAGTCG ACTGCAGCATCCCAGATCACCATCAAGTCTATGCTGCCTCCTTCTCCTGCCCTGAGGGCACCACCTTTGG CAGTCAATGTTCCTTCCAGTGCCGTCACCCTGCACAATTGAAAGGCAACAACAGCCTCCTGACCTGCATG GAGGATGGGCTGTGGTCCTTCCCAGAGGCCCTGTGTGAGCTCATGTGCCTCGCTCCACCCCCTGTGCCCA ATGCAGACCTCCAGACCGCCCGGTGCCGAGAGAATAAGCACAAGGTGGGCTCCTTCTGCAAATACAAATG CAAGCCTGGATACCATGTGCCTGGATCCTCTCGGAAGTCAAAGAAACGGGCCTTCAAGACTCAGTGTACC CAGGATGGCAGCTGGCAGGAGGGAGCTTGTGTTCCTGTGACCTGTGACCCACCTCCACCAAAATTCCATG GGCTCTACCAGTGTACTAATGGCTTCCAGTTCAACAGTGAGTGTAGGATCAAGTGTGAAGACAGTGATGC CTCCCAGGGACTTGGGAGCAATGTCATTCATTGCCGGAAAGATGGCACCTGGAACGGCTCCTTCCATGTC TGCCAGGAGATGCAAGGCCAGTGCTCGGTTCCAAACGAGCTCAACAGCAACCTCAAACTGCAGTGCCCTG ATGGCTATGCCATAGGGTCGGAGTGTGCCACCTCGTGCCTGGACCACAACAGCGAGTCCATCATCCTGCC AATGAACGTGACCGTGCGTGACATCCCCCACTGGCTGAACCCCACACGGGTAGAGAGAGTTGTCTGCACT GCTGGTCTCAAGTGGTATCCTCACCCTGCTCTGATTCACTGTGTCAAAGGCTGTGAGCCCTTCATGGGAG ACAATTATTGTGATGCCATCAACAACCGAGCCTTTTGCAACTATGACGGTGGGGATTGCTGCACCTCCAC AGTGAAGACCAAAAAGGTCACCCCATTCCCTATGTCCTGTGATCTACAAGGTGACTGTGCTTGTCGGGAC CCCCAGGCCCAAGAACACAGCCGGAAAGACCTCCGGGGATACAGCCATGGC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC209767 representing NM_002581
Red=Cloning site Green=Tags(s) MRLWSWVLHLGLLSAALGCGLAERPRRARRDPRAGRPPRPAAGPATCATRAARGRRASPPPPPPPGGAWE AVRVPRRRQQREARGATEEPSPPSRALYFSGRGEQLRLRADLELPRDAFTLQVWLRAEGGQRSPAVITGL YDKCSYISRDRGWVVGIHTISDQDNKDPRYFFSLKTDRARQVTTINAHRSYLPGQWVYLAATYDGQFMKL YVNGAQVATSGEQVGGIFSPLTQKCKVLMLGGSALNHNYRGYIEHFSLWKVARTQREILSDMETHGAHTA LPQLLLQENWDNVKHAWSPMKDGSSPKVEFSNAHGFLLDTSLEPPLCGQTLCDNTEVIASYNQLSSFRQP KVVRYRVVNLYEDDHKNPTVTREQVDFQHHQLAEAFKQYNISWELDVLEVSNSSLRRRLILANCDISKIG DENCDPECNHTLTGHDGGDCRHLRHPAFVKKQHNGVCDMDCNYERFNFDGGECCDPEITNVTQTCFDPDS PHRAYLDVNELKNILKLDGSTHLNIFFAKSSEEELAGVATWPWDKEALMHLGGIVLNPSFYGMPGHTHTM IHEIGHSLGLYHVFRGISEIQSCSDPCMETEPSFETGDLCNDTNPAPKHKSCGDPGPGNDTCGFHSFFNT PYNNFMSYADDDCTDSFTPNQVARMHCYLDLVYQGWQPSRKPAPVALAPQVLGHTTDSVTLEWFPPIDGH FFERELGSACHLCLEGRILVQYASNASSPMPCSPSGHWSPREAEGHPDVEQPCKSSVRTWSPNSAVNPHT VPPACPEPQGCYLELEFLYPLVPESLTIWVTFVSTDWDSSGAVNDIKLLAVSGKNISLGPQNVFCDVPLT IRLWDVGEEVYGIQIYTLDEHLEIDAAMLTSTADTPLCLQCKPLKYKVVRDPPLQMDVASILHLNRKFVD MDLNLGSVYQYWVITISGTEESEPSPAVTYIHGSGYCGDGIIQKDQGEQCDDMNKINGDGCSLFCRQEVS FNCIDEPSRCYFHDGDGVCEEFEQKTSIKDCGVYTPQGFLDQWASNASVSHQDQQCPGWVIIGQPAASQV CRTKVIDLSEGISQHAWYPCTISYPYSQLAQTTFWLRAYFSQPMVAAAVIVHLVTDGTYYGDQKQETISV QLLDTKDQSHDLGLHVLSCRNNPLIIPVVHDLSQPFYHSQAVRVSFSSPLVAISGVALRSFDNFDPVTLS SCQRGETYSPAEQSCVHFACEKTDCPELAVENASLNCSSSDRYHGAQCTVSCRTGYVLQIRRDDELIKSQ TGPSVTVTCTEGKWNKQVACEPVDCSIPDHHQVYAASFSCPEGTTFGSQCSFQCRHPAQLKGNNSLLTCM EDGLWSFPEALCELMCLAPPPVPNADLQTARCRENKHKVGSFCKYKCKPGYHVPGSSRKSKKRAFKTQCT QDGSWQEGACVPVTCDPPPPKFHGLYQCTNGFQFNSECRIKCEDSDASQGLGSNVIHCRKDGTWNGSFHV CQEMQGQCSVPNELNSNLKLQCPDGYAIGSECATSCLDHNSESIILPMNVTVRDIPHWLNPTRVERVVCT AGLKWYPHPALIHCVKGCEPFMGDNYCDAINNRAFCNYDGGDCCTSTVKTKKVTPFPMSCDLQGDCACRD PQAQEHSRKDLRGYSHG SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002581 |
| ORF Size | 4881 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002581.5 |
| RefSeq Size | 11025 bp |
| RefSeq ORF | 4884 bp |
| Locus ID | 5069 |
| UniProt ID | Q13219 |
| Protein Families | Druggable Genome, Protease, Secreted Protein |
| MW | 181.4 kDa |
| Gene Summary | This gene encodes a secreted metalloproteinase which cleaves insulin-like growth factor binding proteins (IGFBPs). Following IGFBP cleavage, insulin growth factors dissociate from IGFBPs and bind to IGF receptors, resulting in activation of the IGF pathway. The encoded protein plays a role in bone formation, inflammation, wound healing and female fertility. Enhanced expression of this protein is associated with diabetic nephropathy in human patients and this protein may promote tumor invasion and growth in various human cancers. [provided by RefSeq, Aug 2017] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
PAPP-A functions as a tumor suppressor and is downregulated in renal cell carcinoma
,Lu, Y;Li, S;Wang, T;Liao, X;Mao, L;Li, Z;,
FEBS open bio
,PubMed ID 33788403
[PAPPA]
|
|
Pregnancy-associated plasma protein A regulates mitosis and is epigenetically silenced in breast cancer
,Loddo, M;Andryszkiewicz, J;Rodriguez-Acebes, S;Stoeber, K;Jones, A;Dafou, D;Apostolidou, S;Wollenschlaeger, A;Widschwendter, M;Sainsbury, R;Tudzarova, S;Williams, GH;,
J. Pathol.
,PubMed ID 24931331
[PAPPA]
|
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209767L1 | Lenti-ORF clone of PAPPA (Myc-DDK-tagged)-Human pregnancy-associated plasma protein A, pappalysin 1 (PAPPA) |
CNY 19,096.00 |
|
| RC209767L2 | Lenti-ORF clone of PAPPA (mGFP-tagged)-Human pregnancy-associated plasma protein A, pappalysin 1 (PAPPA) |
CNY 19,096.00 |
|
| RC209767L3 | Lenti-ORF clone of PAPPA (Myc-DDK-tagged)-Human pregnancy-associated plasma protein A, pappalysin 1 (PAPPA) |
CNY 13,970.00 |
|
| RC209767L4 | Lenti-ORF clone of PAPPA (mGFP-tagged)-Human pregnancy-associated plasma protein A, pappalysin 1 (PAPPA) |
CNY 19,096.00 |
|
| RG209767 | PAPPA (tGFP-tagged) - Human pregnancy-associated plasma protein A, pappalysin 1 (PAPPA) |
CNY 18,296.00 |
|
| SC309898 | PAPPA (untagged)-Human pregnancy-associated plasma protein A, pappalysin 1 (PAPPA) |
CNY 16,704.00 |
