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Nephrin (NPHS1) (NM_004646) Human Tagged ORF Clone
CAT＃: RC217677
NPHS1 (Myc-DDK-tagged)-Human nephrosis 1, congenital, Finnish type (nephrin) (NPHS1)
ORF Plasmid: tGFP
"NM_004646" in other vectors (6)
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CNY 12,960.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CNF; nephrin; NPHN |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC217677 representing NM_004646
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCTGGGGACGACGCTCAGGGCTTCTCTCCTGCTCCTGGGGCTGCTGACTGAAGGCCTGGCGCAGT TGGCGATTCCTGCCTCCGTTCCCCGGGGCTTCTGGGCCCTGCCTGAAAACCTGACGGTGGTGGAGGGGGC CTCAGTGGAGCTGCGTTGTGGGGTCAGCACCCCTGGCAGTGCGGTGCAATGGGCCAAAGATGGGCTGCTC CTGGGCCCCGACCCCAGGATCCCAGGCTTCCCGAGGTACCGCCTGGAAGGGGACCCTGCTAGAGGTGAAT TCCACCTGCACATCGAGGCCTGTGACCTCAGCGATGACGCGGAGTATGAGTGCCAGGTCGGCCGCTCTGA GATGGGGCCCGAGCTCGTGTCTCCCAGAGTGATCCTCTCCATCCTGGTTCCTCCCAAGCTGCTCCTGCTG ACCCCAGAGGCAGGCACCATGGTCACCTGGGTAGCTGGGCAGGAGTACGTGGTCAACTGTGTGTCTGGGG ACGCGAAGCCAGCACCTGACATCACCATTCTCCTGAGTGGACAGACAATATCTGACATCTCTGCAAACGT GAACGAGGGCTCCCAGCAGAAACTCTTCACTGTGGAGGCCACAGCCAGGGTGACACCCCGGAGCTCAGAT AATAGGCAGTTGCTGGTCTGTGAGGCGTCTAGCCCAGCACTGGAGGCCCCCATCAAGGCCTCATTCACCG TGAATGTTCTGTTCCCTCCAGGACCCCCTGTCATCGAGTGGCCAGGCCTGGATGAGGGGCACGTGCGGGC AGGACAGAGCTTGGAGCTGCCGTGCGTGGCCCGAGGGGGTAATCCCTTAGCCACACTGCAGTGGCTGAAG AATGGCCAGCCGGTGTCCACAGCGTGGGGCACAGAGCACACCCAGGCGGTGGCCCGCAGTGTGCTGGTGA TGACCGTGAGGCCAGAAGACCATGGAGCGCAGCTCAGCTGCGAGGCCCACAACAGCGTGTCTGCAGGGAC CCAGGAGCACGGCATCACACTGCAGGTCACCTTTCCCCCTAGTGCCATTATTATCTTGGGATCTGCATCC CAGACTGAGAACAAGAACGTGACACTCTCCTGTGTCAGCAAGTCCAGTCGCCCGCGGGTTCTGCTACGAT GGTGGCTGGGCTGGCGGCAGCTGCTGCCCATGGAGGAGACAGTCATGGATGGACTGCATGGCGGTCACAT CTCCATGTCCAACCTGACATTCCTGGCGCGGCGGGAGGACAACGGTCTGACCCTCACATGTGAGGCCTTC AGTGAAGCCTTCACCAAGGAGACCTTCAAGAAGTCGCTCATCCTGAACGTAAAATATCCCGCCCAGAAAC TGTGGATTGAGGGTCCCCCAGAGGGCCAGAAGCTCCGGGCTGGGACCCGGGTGAGGCTGGTGTGTTTGGC TATCGGGGGCAACCCAGAGCCCTCCCTCATGTGGTACAAGGACTCGCGCACCGTGACCGAGTCGCGGCTG CCGCAGGAGTCGCGGCGCGTGCATCTCGGCAGCGTGGAGAAATCTGGGAGCACCTTCTCCCGAGAGCTGG TGCTGGTCACAGGGCCGTCGGACAACCAGGCCAAGTTCACGTGCAAGGCTGGACAGCTCAGCGCGTCCAC GCAGCTGGCGGTGCAGTTTCCCCCAACTAACGTGACGATCCTGGCCAACGCATCCGCACTGCGCCCGGGA GACGCCTTAAACTTGACATGCGTCAGCGTCAGCAGCAATCCGCCGGTCAACTTGTCCTGGGACAAGGAAG GGGAGAGGCTGGAGGGCGTGGCCGCCCCACCCCGGAGAGCCCCATTCAAAGGCTCCGCCGCCGCCAGGAG CGTCCTTCTGCAAGTGTCATCCCGCGATCATGGCCAGCGCGTGACCTGCCGCGCCCACAGCGCCGAGCTC CGCGAAACCGTGAGCTCCTTCTATCGCCTCAACGTACTGTACCGTCCAGAGTTCCTGGGGGAGCAGGTGC TGGTGGTGACCGCGGTGGAGCAGGGCGAGGCGTTGCTGCCCGTGTCCGTGTCCGCTAACCCCGCCCCCGA GGCCTTCAACTGGACCTTCCGCGGCTATCGCCTCAGTCCAGCGGGCGGCCCCCGGCATCGCATCCTGTCC AGCGGGGCTCTGCATCTGTGGAATGTGACCCGCGCGGACGACGGCCTCTATCAGCTGCACTGCCAGAACT CTGAGGGCACCGCGGAAGCGCGGCTGCGGCTGGACGTGCACTATGCTCCCACCATCCGTGCCCTCCAGGA CCCCACTGAGGTGAACGTCGGGGGTTCTGTGGACATAGTCTGCACTGTCGATGCCAATCCCATCCTCCCG GGCATGTTCAACTGGGAGAGACTGGGAGAAGATGAGGAGGACCAGAGCCTGGATGACATGGAGAAGATAT CCAGGGGACCAACGGGGCGCCTGCGGATTCACCATGCCAAACTGGCCCAGGCTGGCGCTTACCAGTGCAT TGTGGACAATGGGGTGGCGCCTCCAGCACGACGGCTGCTCCGTCTTGTTGTCAGATTTGCCCCCCAGGTG GAGCACCCCACTCCCCTAACTAAGGTGGCTGCAGCTGGAGACAGCACCAGTTCTGCCACCCTCCACTGCC GTGCCCGAGGTGTCCCCAACATCGTTTTCACTTGGACAAAAAACGGGGTCCCTCTGGATCTCCAAGATCC CAGGTACACGGAGCACACATACCACCAGGGTGGTGTCCACAGCAGCCTCCTGACCATTGCCAACGTGTCT GCCGCCCAGGATTACGCCCTCTTCACATGTACAGCCACCAACGCCCTTGGCTCGGACCAAACCAACATTC AACTTGTCAGCATCAGCCGCCCTGACCCTCCATCAGGATTAAAGGTTGTGAGTCTGACCCCACACTCCGT GGGGCTGGAGTGGAAGCCTGGCTTTGATGGGGGCCTGCCACAGAGGTTCTGCATCAGGTATGAGGCCCTG GGGACTCCAGGGTTCCACTATGTGGATGTCGTACCACCCCAGGCCACCACCTTCACGCTGACTGGTCTAC AGCCTTCTACAAGATACAGGGTCTGGCTGCTGGCCAGTAATGCCTTGGGGGACAGTGGACTGGCTGACAA AGGGACCCAGCTTCCCATCACTACCCCAGGTCTCCACCAGCCTTCTGGAGAACCTGAAGACCAGCTGCCC ACAGAGCCACCTTCAGGACCCTCGGGGCTGCCCCTGCTGCCTGTGCTGTTCGCTCTTGGGGGGCTTCTGC TCCTCTCCAATGCCTCCTGTGTCGGGGGGGTCCTCTGGCAGCGGAGACTCAGGCGTCTTGCTGAGGGCAT CTCAGAGAAGACAGAGGCAGGGTCGGAAGAGGACCGAGTCAGGAACGAATATGAGGAGAGCCAGTGGACA GGAGAGCGGGACACTCAGAGCTCCACGGTCAGCACAACAGAGGCAGAGCCGTATTACCGCTCCCTGAGGG ACTTCAGCCCCCAGCTGCCCCCGACGCAGGAGGAGGTGTCTTATTCCCGAGGTTTCACAGGTGAAGATGA GGATATGGCCTTCCCTGGGCACTTGTATGATGAGGTAGAAAGAACGTACCCCCCGTCTGGAGCCTGGGGA CCCCTCTACGATGAAGTGCAGATGGGACCCTGGGACCTCCACTGGCCTGAAGACACATATCAGGATCCAA GAGGAATCTATGACCAGGTGGCCGGAGACTTGGACACTCTGGAACCCGATTCTCTGCCCTTCGAGCTGAG GGGACATCTGGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC217677 representing NM_004646
Red=Cloning site Green=Tags(s) MALGTTLRASLLLLGLLTEGLAQLAIPASVPRGFWALPENLTVVEGASVELRCGVSTPGSAVQWAKDGLL LGPDPRIPGFPRYRLEGDPARGEFHLHIEACDLSDDAEYECQVGRSEMGPELVSPRVILSILVPPKLLLL TPEAGTMVTWVAGQEYVVNCVSGDAKPAPDITILLSGQTISDISANVNEGSQQKLFTVEATARVTPRSSD NRQLLVCEASSPALEAPIKASFTVNVLFPPGPPVIEWPGLDEGHVRAGQSLELPCVARGGNPLATLQWLK NGQPVSTAWGTEHTQAVARSVLVMTVRPEDHGAQLSCEAHNSVSAGTQEHGITLQVTFPPSAIIILGSAS QTENKNVTLSCVSKSSRPRVLLRWWLGWRQLLPMEETVMDGLHGGHISMSNLTFLARREDNGLTLTCEAF SEAFTKETFKKSLILNVKYPAQKLWIEGPPEGQKLRAGTRVRLVCLAIGGNPEPSLMWYKDSRTVTESRL PQESRRVHLGSVEKSGSTFSRELVLVTGPSDNQAKFTCKAGQLSASTQLAVQFPPTNVTILANASALRPG DALNLTCVSVSSNPPVNLSWDKEGERLEGVAAPPRRAPFKGSAAARSVLLQVSSRDHGQRVTCRAHSAEL RETVSSFYRLNVLYRPEFLGEQVLVVTAVEQGEALLPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRILS SGALHLWNVTRADDGLYQLHCQNSEGTAEARLRLDVHYAPTIRALQDPTEVNVGGSVDIVCTVDANPILP GMFNWERLGEDEEDQSLDDMEKISRGPTGRLRIHHAKLAQAGAYQCIVDNGVAPPARRLLRLVVRFAPQV EHPTPLTKVAAAGDSTSSATLHCRARGVPNIVFTWTKNGVPLDLQDPRYTEHTYHQGGVHSSLLTIANVS AAQDYALFTCTATNALGSDQTNIQLVSISRPDPPSGLKVVSLTPHSVGLEWKPGFDGGLPQRFCIRYEAL GTPGFHYVDVVPPQATTFTLTGLQPSTRYRVWLLASNALGDSGLADKGTQLPITTPGLHQPSGEPEDQLP TEPPSGPSGLPLLPVLFALGGLLLLSNASCVGGVLWQRRLRRLAEGISEKTEAGSEEDRVRNEYEESQWT GERDTQSSTVSTTEAEPYYRSLRDFSPQLPPTQEEVSYSRGFTGEDEDMAFPGHLYDEVERTYPPSGAWG PLYDEVQMGPWDLHWPEDTYQDPRGIYDQVAGDLDTLEPDSLPFELRGHLV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004646 |
| ORF Size | 3723 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004646.4 |
| RefSeq Size | 4285 bp |
| RefSeq ORF | 3726 bp |
| Locus ID | 4868 |
| UniProt ID | O60500 |
| Protein Families | Druggable Genome, Transmembrane |
| MW | 134.6 kDa |
| Gene Summary | This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.[provided by RefSeq, Oct 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217677L1 | Lenti ORF clone of Human nephrosis 1, congenital, Finnish type (nephrin) (NPHS1), Myc-DDK-tagged |
CNY 15,360.00 |
|
| RC217677L2 | Lenti ORF clone of Human nephrosis 1, congenital, Finnish type (nephrin) (NPHS1), mGFP tagged |
CNY 14,920.00 |
|
| RC217677L3 | Lenti ORF clone of Human nephrosis 1, congenital, Finnish type (nephrin) (NPHS1), Myc-DDK-tagged |
CNY 14,920.00 |
|
| RC217677L4 | Lenti ORF clone of Human nephrosis 1, congenital, Finnish type (nephrin) (NPHS1), mGFP tagged |
CNY 14,920.00 |
|
| RG217677 | NPHS1 (tGFP-tagged) - Human nephrosis 1, congenital, Finnish type (nephrin) (NPHS1) |
CNY 14,560.00 |
|
| SC315460 | NPHS1 (untagged)-Human nephrosis 1, congenital, Finnish type (nephrin) (NPHS1) |
CNY 12,968.00 |
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