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Nephrin (NPHS1) (NM_004646) Human Untagged Clone
CAT＃: SC315460
NPHS1 (untagged)-Human nephrosis 1, congenital, Finnish type (nephrin) (NPHS1)
CNY 12,968.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CNF; nephrin; NPHN |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_004646 edited
GCCACCATGGCCCTGGGGACGACGCTCAGGGCTTCTCTCCTGCTCCTGGGGCTGCTGACT GAAGGCCTGGCGCAGTTGGCGATTCCTGCCTCCGTTCCCCGGGGCTTCTGGGCCCTGCCT GAAAACCTGACGGTGGTGGAGGGGGCCTCAGTGGAGCTGCGTTGTGGGGTCAGCACCCCT GGCAGTGCGGTGCAATGGGCCAAAGATGGGCTGCTCCTGGGCCCCGACCCCAGGATCCCA GGCTTCCCGAGGTACCGCCTGGAAGGGGACCCTGCTAGAGGTGAATTCCACCTGCACATC GAGGCCTGTGACCTCAGCGATGACGCGGAGTATGAGTGCCAGGTCGGCCGCTCTGAGATG GGGCCCGAGCTCGTGTCTCCCAGAGTGATCCTCTCCATCCTGGTTCCTCCCAAGCTGCTC CTGCTGACCCCAGAGGCAGGCACCATGGTCACCTGGGTAGCTGGGCAGGAGTACGTGGTC AACTGTGTGTCTGGGGACGCGAAGCCAGCACCTGACATCACCATTCTCCTGAGTGGACAG ACAATATCTGACATCTCTGCAAACGTGAACGAGGGCTCCCAGCAGAAACTCTTCACTGTG GAGGCCACAGCCAGGGTGACACCCCGGAGCTCAGATAATAGGCAGTTGCTGGTCTGTGAG GCGTCTAGCCCAGCACTGGAGGCCCCCATCAAGGCCTCATTCACCGTGAATGTTCTGTTC CCTCCAGGACCCCCTGTCATCGAGTGGCCAGGCCTGGATGAGGGGCACGTGCGGGCAGGA CAGAGCTTGGAGCTGCCGTGCGTGGCCCGAGGGGGTAATCCCTTAGCCACACTGCAGTGG CTGAAGAATGGCCAGCCGGTGTCCACAGCGTGGGGCACAGAGCACACCCAGGCGGTGGCC CGCAGTGTGCTGGTGATGACCGTGAGGCCAGAAGACCATGGAGCGCAGCTCAGCTGCGAG GCCCACAACAGCGTGTCTGCAGGGACCCAGGAGCACGGCATCACACTGCAGGTCACCTTT CCCCCTAGTGCCATTATTATCTTGGGATCTGCATCCCAGACTGAGAACAAGAACGTGACA CTCTCCTGTGTCAGCAAGTCCAGTCGCCCGCGGGTTCTGCTACGATGGTGGCTGGGCTGG CGGCAGCTGCTGCCCATGGAGGAGACAGTCATGGATGGACTGCATGGCGGTCACATCTCC ATGTCCAACCTGACATTCCTGGCGCGGCGGGAGGACAACGGTCTGACCCTCACATGTGAG GCCTTCAGTGAAGCCTTCACCAAGGAGACCTTCAAGAAGTCGCTCATCCTGAACGTAAAA TATCCCGCCCAGAAACTGTGGATTGAGGGTCCCCCAGAGGGCCAGAAGCTCCGGGCTGGG ACCCGGGTGAGGCTGGTGTGTTTGGCTATCGGGGGCAACCCAGAGCCCTCCCTCATGTGG TACAAGGACTCGCGCACCGTGACCGAGTCGCGGCTGCCGCAGGAGTCGCGGCGCGTGCAT CTCGGCAGCGTGGAGAAATCTGGGAGCACCTTCTCCCGAGAGCTGGTGCTGGTCACAGGG CCGTCGGACAACCAGGCCAAGTTCACGTGCAAGGCTGGACAGCTCAGCGCGTCCACGCAG CTGGCGGTGCAGTTTCCCCCAACTAACGTGACGATCCTGGCCAACGCATCCGCACTGCGC CCGGGAGACGCCTTAAACTTGACATGCGTCAGCGTCAGCAGCAATCCGCCGGTCAACTTG TCCTGGGACAAGGAAGGGGAGAGGCTGGAGGGCGTGGCCGCCCCACCCCGGAGAGCCCCA TTCAAAGGCTCCGCCGCCGCCAGGAGCGTCCTTCTGCAAGTGTCATCCCGCGATCATGGC CAGCGCGTGACCTGCCGCGCCCACAGCGCCGAGCTCCGCGAAACCGTGAGCTCCTTCTAT CGCCTCAACGTACTGTACCGTCCAGAGTTCCTGGGGGAGCAGGTGCTGGTGGTGACCGCG GTGGAGCAGGGCGAGGCGTTGCTGCCCGTGTCCGTGTCCGCTAACCCCGCCCCCGAGGCC TTCAACTGGACCTTCCGCGGCTATCGCCTCAGTCCAGCGGGCGGCCCCCGGCATCGCATC CTGTCCAGCGGGGCTCTGCATCTGTGGAATGTGACCCGCGCGGACGACGGCCTCTATCAG CTGCACTGCCAGAACTCTGAGGGCACCGCGGAAGCGCGGCTGCGGCTGGACGTGCACTAT GCTCCCACCATCCGTGCCCTCCAGGACCCCACTGAGGTGAACGTCGGGGGTTCTGTGGAC ATAGTCTGCACTGTCGATGCCAATCCCATCCTCCCGGGCATGTTCAACTGGGAGAGACTG GGAGAAGATGAGGAGGACCAGAGCCTGGATGACATGGAGAAGATATCCAGGGGACCAACG GGGCGCCTGCGGATTCACCATGCCAAACTGGCCCAGGCTGGCGCTTACCAGTGCATTGTG GACAATGGGGTGGCGCCTCCAGCACGACGGCTGCTCCGTCTTGTTGTCAGATTTGCCCCC CAGGTGGAGCACCCCACTCCCCTAACTAAGGTGGCTGCAGCTGGAGACAGCACCAGTTCT GCCACCCTCCACTGCCGTGCCCGAGGTGTCCCCAACATCGTTTTCACTTGGACAAAAAAC GGGGTCCCTCTGGATCTCCAAGATCCCAGGTACACGGAGCACACATACCACCAGGGTGGT GTCCACAGCAGCCTCCTGACCATTGCCAACGTGTCTGCCGCCCAGGATTACGCCCTCTTC ACATGTACAGCCACCAACGCCCTTGGCTCGGACCAAACCAACATTCAACTTGTCAGCATC AGCCGCCCTGACCCTCCATCAGGATTAAAGGTTGTGAGTCTGACCCCACACTCCGTGGGG CTGGAGTGGAAGCCTGGCTTTGATGGGGGCCTGCCACAGAGGTTCTGCATCAGGTATGAG GCCCTGGGGACTCCAGGGTTCCACTATGTGGATGTCGTACCACCCCAGGCCACCACCTTC ACGCTGACTGGTCTACAGCCTTCTACAAGATACAGGGTCTGGCTGCTGGCCAGTAATGCC TTGGGGGACAGTGGACTGGCTGACAAAGGGACCCAGCTTCCCATCACTACCCCAGGTCTC CACCAGCCTTCTGGAGAACCTGAAGACCAGCTGCCCACAGAGCCACCTTCAGGACCCTCG GGGCTGCCCCTGCTGCCTGTGCTGTTCGCTCTTGGGGGGCTTCTGCTCCTCTCCAATGCC TCCTGTGTCGGGGGGGTCCTCTGGCAGCGGAGACTCAGGCGTCTTGCTGAGGGCATCTCA GAGAAGACAGAGGCAGGGTCGGAAGAGGACCGAGTCAGGAACGAATATGAGGAGAGCCAG TGGACAGGAGAGCGGGACACTCAGAGCTCCACGGTCAGCACAACAGAGGCAGAGCCGTAT TACCGCTCCCTGAGGGACTTCAGCCCCCAGCTGCCCCCGACGCAGGAGGAGGTGTCTTAT TCCCGAGGTTTCACAGGTGAAGATGAGGATATGGCCTTCCCTGGGCACTTGTATGATGAG GTAGAAAGAACGTACCCCCCGTCTGGAGCCTGGGGACCCCTCTACGATGAAGTGCAGATG GGACCCTGGGACCTCCACTGGCCTGAAGACACATATCAGGATCCAAGAGGAATCTATGAC CAGGTGGCCGGAGACTTGGACACTCTGGAACCCGATTCTCTGCCCTTCGAGCTGAGGGGA CATCTGGTGTAA |
| Restriction Sites | Please inquire |
| ACCN | NM_004646 |
| Insert Size | 3800 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004646.1, NP_004637.1 |
| RefSeq Size | 4285 bp |
| RefSeq ORF | 3726 bp |
| Locus ID | 4868 |
| UniProt ID | O60500 |
| Protein Families | Druggable Genome, Transmembrane |
| Gene Summary | This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.[provided by RefSeq, Oct 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217677 | NPHS1 (Myc-DDK-tagged)-Human nephrosis 1, congenital, Finnish type (nephrin) (NPHS1) |
CNY 12,960.00 |
|
| RC217677L1 | Lenti ORF clone of Human nephrosis 1, congenital, Finnish type (nephrin) (NPHS1), Myc-DDK-tagged |
CNY 15,360.00 |
|
| RC217677L2 | Lenti ORF clone of Human nephrosis 1, congenital, Finnish type (nephrin) (NPHS1), mGFP tagged |
CNY 14,920.00 |
|
| RC217677L3 | Lenti ORF clone of Human nephrosis 1, congenital, Finnish type (nephrin) (NPHS1), Myc-DDK-tagged |
CNY 14,920.00 |
|
| RC217677L4 | Lenti ORF clone of Human nephrosis 1, congenital, Finnish type (nephrin) (NPHS1), mGFP tagged |
CNY 14,920.00 |
|
| RG217677 | NPHS1 (tGFP-tagged) - Human nephrosis 1, congenital, Finnish type (nephrin) (NPHS1) |
CNY 14,560.00 |
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