PHF17 (JADE1) (NM_001287440) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	PHF17
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239667 representing NM_001287440 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAACGAGGTCGCCTTCCCAGCAGCAGTGAGGATTCTGACGACAATGGCAGCCTGTCAACTACTTGGT CCCAGAATTCCCGATCCCAGCATAGGAGAAGCTCCTGCTCCAGACATGAAGATCGAAAGCCTTCAGAGGT GTTTAGGACAGACCTGATCACTGCCATGAAGTTGCATGACTCCTACCAGCTGAATCCGGATGAGTACTAT GTGTTGGCAGATCCCTGGAGACAGGAATGGGAGAAAGGGGTCCAGGTGCCTGTGAGCCCGGGGACCATCC CTCAGCCTGTGGCCAGGGTTGTGTCTGAAGAGAAATCCCTCATGTTCATCAGGCCCAAGAAGTACATCGT GTCATCAGGCTCTGAGCCTCCCGAGTTGGGCTATGTGGACATCCGGACGCTGGCTGACAGCGTGTGTCGC TATGACCTCAATGACATGGATGCTGCATGGCTGGAACTGACCAATGAAGAATTTAAGGAGATGGGAATGC CTGAACTAGATGAATACACCATGGAGAGGGTCCTAGAGGAATTTGAGCAGCGATGCTACGACAATATGAA TCATGCCATAGAGACTGAGGAAGGCCTGGGGATCGAATATGATGAAGATGTTGTCTGTGATGTCTGCCAG TCTCCTGATGGTGAGGACGGCAATGAGATGGTGTTCTGTGACAAATGCAACATCTGTGTGCACCAGGCCT GTTATGGAATCCTCAAGGTACCAGAGGGCAGCTGGCTGTGCCGGACATGTGCCCTGGGGGTTCAGCCAAA ATGTCTGCTGTGTCCGAAGAAGGGTGGAGCTATGAAGCCCACCCGTAGCGGAACCAAGTGGGTCCACGTT AGCTGTGCTCTGTGGATCCCTGAGGTGAGCATTGGCAGCCCAGAGAAGATGGAGCCCATCACCAAGGTGT CACACATTCCCAGCAGCCGGTGGGCGCTAGTGTGCAGCCTCTGCAATGAGAAGTTTGGGGCCTCTATACA GTGCTCTGTGAAGAACTGCCGCACAGCCTTCCATGTGACCTGTGCTTTTGACCGGGGCCTGGAGATGAAG ACCATCTTAGCAGAGAATGATGAAGTCAAGTTCAAGTCCTATTGCCCAAAGCACAGCTCACATAGGAAAC CCGAGGAGAGTCTTGGCAAGGGGGCTGCACAGGAGAATGGGGCCCCTGAGTGTTCCCCCCGGAATCCGCT GGAGCCCTTTGCCAGCCTTGAGCAGAACCGGGAGGAGGCCCACCGGGTGAGTGTCCGTAAGCAGAAGCTG CAGCAGTTGGAGGATGAGTTCTACACCTTCGTCAACCTGCTGGATGTTGCCAGGGCTCTGCGGCTGCCTG AGGAAGTAGTGGATTTCCTGTACCAGTACTGGAAGTTGAAGAGGAAGGTCAACTTCAACAAGCCCCTGAT CACCCCAAAGAAAGATGAAGAGGACAATCTAGCCAAGCGGGAGCAGGATGTCTTATTTAGGAGGCTGCAG CTGTTCACGCACCTGCGGCAGGACCTGGAGAGGGTTCGGAACCTCACTTACATGGTGACCCGCAGGGAAA AGATTAAACGGTCTGTGTGCAAAGTCCAGGAACAGATATTCAATCTTTACACTAAGCTTTTGGAGCAAGA AAGAGTTTCAGGTGTGCCTTCTTCCTGCTCCTCCTCCTCACTGGAAAACATGCTTTTGTTTAACAGTCCT TCTGTTGGCCCTGATGCTCCCAAGATAGAGGACTTGAAGTGGCATTCTGCATTCTTCAGAAAACAAATGG GTACTTCCTTGGTTCATTCGCTGAAAAAGCCCCATAAGCGAGATCCTTTGCAGAATAGCCCTGGAAGTGA AGGCAAAACCCTGCTGAAGCAGCCAGACCTGTGTGGTAGAAGGGAGGGGATGGTGGTCCCAGAGAGCTTT TTGGGTTTAGAAAAGACCTTTGCAGAAGCACGTCTCATATCAGCACAACAGAAAAATGGTGTGGTGATGC CAGACCATGGGAAAAGAAGAGACAATCGTTTTCATTGTGATCTCATTAAAGGAGACTTAAAGGACAAATC TTTTAAACAGAGTCACAAGCCTCTCAGGTCCACAGATGTGTCCCAGAGGCATCTGGACAACACAAGAGCT GCCACCTCCCCTGGAGTGGGGCAGTCAGCACCTGGCACAAGGAAGGAGATAGTGCCCAAGTGCAATGGCT CCCTAATCAAAGTAAACTATAATCAGACTGCAGTCAAAGTGCCTACAACACCTGCCAGCCCAGTGAAAAA CTGGGGAGGATTCCGGATTCCAAAGAAGGGGGAACGGCAGCAGCAGGGAGAGGCCCACGATGGGGCCTGC CACCAGCACTCAGACTACCCATATTTGGGCTTAGGCCGAGTTCCAGCCAAGGAAAGGGCAAAAAGCAAAT TAAAATCCGACAATGAGAATGACGGGTATGTCCCCGATGTGGAAATGAGTGACTCAGAGAGTGAGGCATC AGAAAAGAAATGTATACACACCAGCAGCACTATCAGCAGAAGGACAGACATTATCAGGAGAAGCATCTTG GCTTCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239667 representing NM_001287440 Red=Cloning site Green=Tags(s) MKRGRLPSSSEDSDDNGSLSTTWSQNSRSQHRRSSCSRHEDRKPSEVFRTDLITAMKLHDSYQLNPDEYY VLADPWRQEWEKGVQVPVSPGTIPQPVARVVSEEKSLMFIRPKKYIVSSGSEPPELGYVDIRTLADSVCR YDLNDMDAAWLELTNEEFKEMGMPELDEYTMERVLEEFEQRCYDNMNHAIETEEGLGIEYDEDVVCDVCQ SPDGEDGNEMVFCDKCNICVHQACYGILKVPEGSWLCRTCALGVQPKCLLCPKKGGAMKPTRSGTKWVHV SCALWIPEVSIGSPEKMEPITKVSHIPSSRWALVCSLCNEKFGASIQCSVKNCRTAFHVTCAFDRGLEMK TILAENDEVKFKSYCPKHSSHRKPEESLGKGAAQENGAPECSPRNPLEPFASLEQNREEAHRVSVRKQKL QQLEDEFYTFVNLLDVARALRLPEEVVDFLYQYWKLKRKVNFNKPLITPKKDEEDNLAKREQDVLFRRLQ LFTHLRQDLERVRNLTYMVTRREKIKRSVCKVQEQIFNLYTKLLEQERVSGVPSSCSSSSLENMLLFNSP SVGPDAPKIEDLKWHSAFFRKQMGTSLVHSLKKPHKRDPLQNSPGSEGKTLLKQPDLCGRREGMVVPESF LGLEKTFAEARLISAQQKNGVVMPDHGKRRDNRFHCDLIKGDLKDKSFKQSHKPLRSTDVSQRHLDNTRA ATSPGVGQSAPGTRKEIVPKCNGSLIKVNYNQTAVKVPTTPASPVKNWGGFRIPKKGERQQQGEAHDGAC HQHSDYPYLGLGRVPAKERAKSKLKSDNENDGYVPDVEMSDSESEASEKKCIHTSSTISRRTDIIRRSIL AS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001287440
ORF Size	2526 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001287440.2
RefSeq Size	5687 bp
RefSeq ORF	2529 bp
Locus ID	79960
UniProt ID	Q6IE81
Protein Families	Druggable Genome, Transcription Factors
MW	96 kDa
Gene Summary	Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Transcriptional coactivator, it may also promote acetylation of nucleosomal histone H4 by KAT5. Promotes apoptosis. May act as a renal tumor suppressor. Negatively regulates canonical Wnt signaling; at least in part, cooperates with NPHP4 in this function.[UniProtKB/Swiss-Prot Function]

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