PPM1K (AK057622) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK057622, the custom clone sequence may differ by one or more nucleotides CATTTTTTATTGTGAAATAATTTAAACATAAGATTGAGAATAATATACTATCTGTAGACCATCCACCAAC ATCATCAAACCTTAGTATTCTGCCATCTTTTCAAGTTGATTTTAAAAGAATAAAGTAATAGAACAAAATC CTATGGAGACAAATTTTTTATTTGTGTGGAAAATTCCCAGAAACAGCTCTACCTGCAGTGGGGTAGGAGT TCCCCTGGGTAACACCCTGTAATGCCAAGAGAGGTTTTAGGATTGAGTTTCAGGGTGGAGTCCAGAAATG GGGGTGTAAAGTAATCGTTTCAAAGAAGTGTAGTCTCAAGTGTCGTTGGGTTGTTAGGGTTGGGGAAAGG AGAGCTGCAGCTGTGAATGTAAATATATAGATCACTCGTTCAGAAATCAGGGCTGCTTGTCACAAATGCA GTGCTGCCAGGTTCCTGAGATCATACCCACATACCCTCCTAGTGAGAGAGTCTGCTAGGATGGGGAGCAT CAAAACTGGCTTATCTTTGTGCCTCTGGGGTGTTTGCACACCAATTTATATTTACCATAGTAATATAGTA TGTAAAGCTTAGTAGGTTAAGAAATGTTAAAATAAAACTTAGATTTATCCTAGATTATGTTTTTGTTAAA ACTTGGCCATTCTTTGGAAACAGAACTATTTTAAGTATTCTATTAGCCTGTTATTTTTTCCTGAAGAGAC TTTGTTGCTATTGTTAATATTTATCCCTACCTTTTCTTTAAAGCAATAGAGTTAATGAAGGGAAGTATAT TTATTTATACCTGTATTCTGTGTTCTTGCTACTAGAAGTGTAACTTGGGGACCAGCAGCTTCTGTATCAC CAGGGTGTTGGTTAGAAATGCAGAGTGACTCCACCCCATAGCCACTGGGTCAGAACATGCATTTTAACAA GTGATTGCAGGTTCAAGTTTAAGAGATACATGTTCTGTGTATGTGCACACTGTTAAATTTTTATAGAATT TAAATGCTGTGAATGCGGTAGGAAAATCGATTCATTTTTGATCCACTGTTCTTAGAAGCAGGCTACTGGA TGCCTACCCAATATATAACATAAATATATGTATTTTCTGTCAAATCCTTTATGAGCTTCATTTTTGGTAA TATTTGATGACATATCAGTTATATTCAAAATTTCTGATGGTCTCTCTCTCTCTGTCTCTCTCCCTCTCCT CCTCTGCCCCTCTCTCCCTCTGTCCCCTGTGACTTGCATCATTTTTCTTGAAGACATTCCATTATTGTTC CATTGACCTTTCCCTCATCACTGAGTCCTTTGGAGCTGAGTTATGTCAACAGCTGCCTTAATTACTTTGG TCAGAAGTGGTGGGAACCAGGTGAGAAGGAGAGTGCTGCTAAGCTCCCGCCTGCTGCAGGACGACAGGCG GGTGACACCCACGTGCCACAGCTCCACTTCAGAGCCTAGGTGTTCTCGGTTTGACCCAGATGGTAGTGGG AGTCCAGCTACCTGGGACAATTTTGGGATCTGGGATAACCGCATTGATGAGCCAATTCTGCTGCCACCCA GCATTAAGTATGGCAAGCCAATTCCCAAAATCAGCTTGGAAAATGTGGGGTGCGCCTCACAGATTGGCAA ACGGAAAGAGAATGAAGATCGGTTTGACTTCGCTCAGCTGACAGATGAGGTCCTGTACTTTGCAGTGTAT GATGGACACGGTGGACCTGCAGCAGCTGATTTCTGTCATACCCACATGGAGAAATGTATTATGTATGTAC AATGACTTTCACTCCTGACTTTGCAGTTCCTATACTTCCTACTTAAACTTTCACCCTTTGGCTGAGGCTT CCAGCGTAAACATAGTTACTTGAGTGGTTGCATGCTGCTGCTTCACGAATGAGCAGTAGTTTAAGTGTGC CATTTATTACTTATGGTTATCACAATTTCAGAAATGCATGTTTTAACAACTCCCCTGGGGAAAGCATTAA CCAAAATCTATAGCACTTAATTTGTGATCATTTTAAGTGAAGTGGCATAATGTACATGAGATAACCTATG AGAAGCATTTACTCCCATCAGTGTAGGACAGATAAGTGCTTCAGTAAATGGTAATTACTCTTTCAAGTGG TGTGAATAGCTTTGAATACCTGACTTGTTGAAAATAGCTTTGGATGATTGGCATTAGTGTGGAAAAATGC ATTTAATGCAGGCATTTTGGAGCTCTTCCGTTTATTTACTTCAACTAGTATTAATGCATTTTTGAGGATC ACATTACTGCTAAAATTGAAAGAGGGAGAACATGTGATGGAGTTACGACCATGTGACTATTTTTGACTTC CCAGTGACCATTTCTTGACTTCCTGTGTTTACCGCATTCCACGAAAGAACTCACAATGGGAAGGAACTCT CGACCTCTCAGGGTTGTGGTAGTGAGGATCAATTCTTCAAGGACAAGGACTTGGACTTTCATTGTCGTGC CCTTAAAAGCTTATCACAGTGCCTGATACTTTGTGGATTCTCAATAGGTATTCCTTAGCCAGTAGGTCTT CAGCATTTGACAGGAAAATCCTGAGGCCCGTTGTGTGGAATGACTGCTAGCTAGAGTAACAGTGAGGAGA AAAACCTATTGTAAAATGATTGTTTAAGGCTCTTTTTTTCCTCCCCACAAAGGGATTTGCTTCCTAAGGA GAAGAACTTGGAAACTCTGTTGACCTTGGCTTTTCTAGAAATAGATAAAGCCTTTTCGAGTCATGCCCGC CTGTCTGCTGATGGTATGTAAAACTACCACATTCCTTGGATCCTAGGGAGTACATGGGGTGGGGGAGGAG TAAAAAGGAAACACTTTAAGTCCTTTGGGGGAGGGGATTTTAATGGACAATTGTTTGTAAGGATTTATGT AGAAATGAGTTTATATGAAGAATCACATTAAATGCTGTTGGGAACTCCAATTAATGTTAATACCTCACTT TTAAATGAAATATATTGGAGAATTTTTCAGAAAGAGAAGCCCCGAAACAGTTCTGATGTTGTACTTTAGT TTTTAAAAGATTTTAAAAATGTTTAATCTGTTTGCTTATTTTATGGACAAAATGTCCATACTATGAGTTT TGTTTTGGGTTTTTGGTTTTTTTTTTAGATGGATCTGGTGACCAAGTGCTGAAATACACTACAGGGTAAT CACATCTAGCGAGGACAGTCATTGTCCAAC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK057622 unedited GGTTCACATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGCAGGCAAGG CAGTGGCCGCTTTGACTGCTTGCTTCGGAGATCCGAGACGACGGAGAAGGCACTCTTATT TACCGACCAAGAAAGCTCCTCCCCCGTCCTCCGTTAGCTAATTAAAACATTTTTCAGGGA CGTAGCCATCCAGAGACATTCCATTATTGTTCCATTGACCTTTCCCTCATCACTGAGTCC TTTGGAGCTGAGTTATGTCAACAGCTGCCTTAATTACTTTGGTCAGAAGTGGTGGGAACC AGGTGAGAAGGAGAGTGCTGCTAAGCTCCCGCCTGCTGCAGGACGACAGGCGGGTGACAC CCACGTGCCACAGCTCCACTTCAGAGCCTAGGTGTTCTCGGTTTGACCCAGATGGTAGTG GGAGTCCAGCTACCTGGGACAATTTTGGGATCTGGGATAACCGCATTGATGAGCCAATTC TGCTGCCACCCAGCATTAAGTATGGCAAGCCAATTCCCAAAATCAGCTTGGAAAATGTGG GGTGCGCCTCACAGATTGGCAAACGGAAAGAGAATGAAGATCGGTTTGACTTCGCTCAGC TGACAGATGAGGTCCTGTACTTTGCAGTGTATGATGGACACGGTGGACCTGCAGCAGCTG ATTTCTGTCATACCCACATGGAGAAATGTATTATGTATGTACAATGACTNTCACTCCTGA CTNTGCAGTTCCTATACTTCCTACTTAAACTTTCACCCTTTGGCTGNAGCTTNCAGCGTA NACATAGTTACTTGAGTGGTTGCATGCTGCTGCTTCACGAATGAGCAGTAGTTTAGTGTG CCATTTATTACTTATGGGTATCACAAATTCAGAATGCATGNTTTAACAACTNCCTGGGGA AAGCATTACCAAATCTTAGCACTAATNTGTGACATTTAAGTGAA
Restriction Sites	NotI-NotI
ACCN	AK057622
Insert Size	4200 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AK057622.1
RefSeq Size	3180 bp
RefSeq ORF	3180 bp
Locus ID	152926
Protein Families	Phosphatase
Gene Summary	This gene encodes a member of the PPM family of Mn2+/Mg2+-dependent protein phosphatases. The encoded protein, essential for cell survival and development, is targeted to the mitochondria where it plays a key role in regulation of the mitochondrial permeability transition pore. [provided by RefSeq, Sep 2012]

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