TFIISH (TCEA3) (BC019675) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC019675, the custom clone sequence may differ by one or more nucleotides GATGGAGGCAAGGTTACAGGTTGACTCAATAGGTAGTCACAGTTCTTGGCATTTTGAATATTATTTGCAC ATTTCACTTTGGAAACACAGTAGACTCTATCGAGGCCAGGCTTGGTCACTTCCTTCCCATCAGTTCTGTG TTGAGTTGCCATCTCTCAAGATGGTAAATTGTTTCCTGCTCGCTTTTATCCTCTCTGGTTCTCTTACTTT TAGGACCCTTTTTTCAGTAAGTAATTTGTTTATTAGCCAGGACCCAAGACTAAGTTATTTACATGTCCAT TGTAAATGCAATGTAAATGAGAGTTCTGATAAAATATTTTTGTATTTTGTAATATCAAAGGAATTTCTAT ATCGTAGGACTCAGTGGGGACTTGCATGCACATCCACCATTGTCTCTGAGTAGTATTGAAATGTGGCCCG GCACCACCACTGACCTCTTCCAGTGCAGCAAATGCAAGAAGAAGAACTGCACCTATAACCAGGTGCAGAC ACGCAGTGCTGATGAGCCCATGACTACCTTTGTCTTATGCAATGAATGTGGCAATCGCTGGAAGTTCTGC TGATGGAACAGCCAGCCATGAACAAGGTGAGGAAGAAGAAAGAGGAAGCGCTGAATTATCTGAACTGGAG AAGCAATAAAAATTAAAGTGAAGGAAAATACTGAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC019675 unedited TTGCAAATTTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGGAAAAAAG GGAAAATGCTGTCAGTAGATGGAGGCAAGGTTACAGGTTGACTCAATAGGTAGTCACAGT TCTTGGCATGTTGAATATTATTTGCACATTTCACTTTGGAAACACAGTAGACTCTATCGA GGCCAGGCTTGGTCACTTCCTTCCCATCAGTTCTGTGTTGAGTTGCCATCTCTCAAGATG GTAAATTGTTTCCTGCTCGCTTTTATCCTCTCTGGTTCTCTTACTTTTAGGACCCTTTTT TCAGTAAGTAATTTGTTTATTAGCCAGGACCCAAGACTAAGTTATTTACATGTCCATTGT AAATGCAATGTAAATGAGAGTTCTGATAAAATATTTTTGTATTTTGTAATATCAAAGGAA TTTCTATATCGTAGGACTCAGTGGGGGACTTGCATGCACATCCACCATTGTCTCTGAGTA GTATTGAAATGTGGCCCGGTACCACCTTTNTTTTTTTTTTTTTTTTTCTATACAAATTTG TTACATGTCAGTGAGACCTTTTTCAAAGGAACATATTCAATTTGGCTTTTTGTGGCTGAA AAAAATAACTGTTAAACCCAAACATTTATGCTCCGTCCCTCTTAGGAACCTCCTTAATCT GTCCCACCCCAGTAGAATTTTTTCTACAAGTGGTAGTAATTTTTTTTAAATGAAATGTAA TTTTGCCATTAAAAAACAACCGGGTCAGTAAATTCTGTGAAAAGCCCTCCTGCTGGAATG TTGAAGTACTTATGGTGAACTATCCCTGGGGAAAGTAAATGACTGGGAGTGGTGCATGGG GGATGTCACCTGACCTGGTTTGAATGGGCATTAGTTCTGNGCCTATGTTTCTGAATGGT
Restriction Sites	NotI-NotI
ACCN	BC019675
Insert Size	4700 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC019675.1
RefSeq Size	681 bp
RefSeq ORF	681 bp
Locus ID	6920
Protein Families	Transcription Factors
Gene Summary	Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.[UniProtKB/Swiss-Prot Function]

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