CRNKL1 (NM_001278627) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CLF; Clf1; CRN; HCRN; MSTP021; SYF3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336805 representing NM_001278627. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAAATGAAGAATCGCCAAGTCAACCATGCTCGAAATATCTGGGACCGGGCCATAACAACGCTGCCT CGAGTTAATCAGTTCTGGTACAAGTACACGTACATGGAGGAAATGTTGGGAAACGTTGCCGGTGCCCGG CAGGTGTTTGAGCGCTGGATGGAGTGGCAGCCTGAGGAGCAAGCCTGGCACTCCTACATCAACTTTGAG CTGAGATACAAAGAGGTGGATCGGGCCCGCACCATTTATGAGCGATTTGTCCTCGTGCACCCTGATGTT AAGAACTGGATCAAGTATGCCCGCTTTGAAGAAAAACATGCTTATTTTGCCCATGCACGGAAAGTGTAT GAGAGAGCTGTGGAATTCTTTGGAGATGAACATATGGATGAGCACCTTTATGTTGCCTTTGCCAAGTTT GAAGAAAATCAGAAAGAGTTTGAAAGGGTACGAGTGATTTACAAGTATGCCCTGGACAGAATTTCAAAA CAAGATGCCCAAGAACTCTTTAAAAATTATACCATCTTTGAGAAGAAGTTTGGTGATAGGCGGGGTATT GAAGATATCATTGTGAGCAAACGGAGATTCCAGTACGAAGAAGAAGTGAAGGCGAATCCACACAATTAT GATGCATGGTTTGATTACTTGCGCTTGGTAGAAAGTGACGCAGAAGCTGAAGCCGTGAGAGAAGTCTAT GAAAGGGCCATTGCCAATGTCCCACCCATTCAGGAGAAGAGGCACTGGAAGCGCTACATTTATCTTTGG ATCAACTATGCACTCTATGAAGAATTGGAGGCAAAGGATCCTGAGAGGACAAGACAGGTGTATCAAGCC TCTTTGGAACTAATTCCTCACAAAAAGTTCACATTTGCCAAAATGTGGATACTGTATGCACAGTTTGAA ATACGACAGAAGAATCTGTCATTAGCCAGAAGAGCATTGGGAACTTCCATAGGCAAATGTCCAAAGAAC AAATTATTTAAAGTTTACATAGAATTGGAGCTACAGCTTCGAGAATTTGACAGATGCCGGAAGCTTTAT GAAAAGTTCCTGGAATTTGGACCTGAAAATTGTACCTCATGGATTAAATTCGCTGAATTAGAGACAATC CTTGGTGATATTGACAGAGCACGGGCAATCTATGAATTAGCCATCAGTCAGCCACGTTTAGACATGCCA GAGGTGCTTTGGAAATCATATATTGATTTTGAAATTGAGCAGGAAGAAACAGAAAGAACACGAAACCTT TACCGGCGGTTGCTTCAACGGACGCAGCATGTCAAGGTATGGATCAGCTTTGCTCAGTTTGAGTTGTCT TCAGGAAAAGAAGGAAGTTTGACTAAATGCAGACAAATTTATGAAGAAGCTAACAAAACCATGCGAAAC TGTGAAGAAAAGGAAGAGAGACTTATGCTGCTGGAATCTTGGCGAAGTTTTGAAGAAGAATTTGGAACA GCTTCAGATAAGGAGAGAGTAGACAAACTCATGCCAGAGAAAGTCAAGAAGAGAAGAAAGGTCCAGACT GATGATGGGTCTGATGCAGGCTGGGAAGAATACTTTGATTACATCTTTCCAGAAGATGCTGCCAACCAA CCTAACCTCAAACTCCTGGCCATGGCCAAACTGTGGAAGAAACAGCAGCAGGAAAAGGAGGATGCTGAG CACCATCCAGATGAGGACGTCGATGAGAGTGAATCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001278627
Insert Size	1695 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278627.1
RefSeq Size	4100 bp
RefSeq ORF	1695 bp
Locus ID	51340
UniProt ID	Q9BZJ0
Protein Pathways	Spliceosome
MW	68.6 kDa
Gene Summary	The crooked neck (crn) gene of Drosophila is essential for embryogenesis and is thought to be involved in cell cycle progression and pre-mRNA splicing. A protein encoded by this human locus has been found to localize to pre-mRNA splicing complexes in the nucleus and is necessary for pre-mRNA splicing. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2013] Transcript Variant: This variant (4) uses two alternate splice sites in the 5' UTR, compared to variant 1. These differences result in predicted translation initiation at a downstream in-frame start site through ribosomal re-initiation and/or leaky scanning. The encoded isoform (c) is shorter than isoform a. Both variants 3 and 4 encode the same isoform (c).

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