CRNKL1 (NM_001278627) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CLF; Clf1; CRN; HCRN; MSTP021; SYF3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238911 representing NM_001278627 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAATGAAGAATCGCCAAGTCAACCATGCTCGAAATATCTGGGACCGGGCCATAACAACGCTGCCTC GAGTTAATCAGTTCTGGTACAAGTACACGTACATGGAGGAAATGTTGGGAAACGTTGCCGGTGCCCGGCA GGTGTTTGAGCGCTGGATGGAGTGGCAGCCTGAGGAGCAAGCCTGGCACTCCTACATCAACTTTGAGCTG AGATACAAAGAGGTGGATCGGGCCCGCACCATTTATGAGCGATTTGTCCTCGTGCACCCTGATGTTAAGA ACTGGATCAAGTATGCCCGCTTTGAAGAAAAACATGCTTATTTTGCCCATGCACGGAAAGTGTATGAGAG AGCTGTGGAATTCTTTGGAGATGAACATATGGATGAGCACCTTTATGTTGCCTTTGCCAAGTTTGAAGAA AATCAGAAAGAGTTTGAAAGGGTACGAGTGATTTACAAGTATGCCCTGGACAGAATTTCAAAACAAGATG CCCAAGAACTCTTTAAAAATTATACCATCTTTGAGAAGAAGTTTGGTGATAGGCGGGGTATTGAAGATAT CATTGTGAGCAAACGGAGATTCCAGTACGAAGAAGAAGTGAAGGCGAATCCACACAATTATGATGCATGG TTTGATTACTTGCGCTTGGTAGAAAGTGACGCAGAAGCTGAAGCCGTGAGAGAAGTCTATGAAAGGGCCA TTGCCAATGTCCCACCCATTCAGGAGAAGAGGCACTGGAAGCGCTACATTTATCTTTGGATCAACTATGC ACTCTATGAAGAATTGGAGGCAAAGGATCCTGAGAGGACAAGACAGGTGTATCAAGCCTCTTTGGAACTA ATTCCTCACAAAAAGTTCACATTTGCCAAAATGTGGATACTGTATGCACAGTTTGAAATACGACAGAAGA ATCTGTCATTAGCCAGAAGAGCATTGGGAACTTCCATAGGCAAATGTCCAAAGAACAAATTATTTAAAGT TTACATAGAATTGGAGCTACAGCTTCGAGAATTTGACAGATGCCGGAAGCTTTATGAAAAGTTCCTGGAA TTTGGACCTGAAAATTGTACCTCATGGATTAAATTCGCTGAATTAGAGACAATCCTTGGTGATATTGACA GAGCACGGGCAATCTATGAATTAGCCATCAGTCAGCCACGTTTAGACATGCCAGAGGTGCTTTGGAAATC ATATATTGATTTTGAAATTGAGCAGGAAGAAACAGAAAGAACACGAAACCTTTACCGGCGGTTGCTTCAA CGGACGCAGCATGTCAAGGTATGGATCAGCTTTGCTCAGTTTGAGTTGTCTTCAGGAAAAGAAGGAAGTT TGACTAAATGCAGACAAATTTATGAAGAAGCTAACAAAACCATGCGAAACTGTGAAGAAAAGGAAGAGAG ACTTATGCTGCTGGAATCTTGGCGAAGTTTTGAAGAAGAATTTGGAACAGCTTCAGATAAGGAGAGAGTA GACAAACTCATGCCAGAGAAAGTCAAGAAGAGAAGAAAGGTCCAGACTGATGATGGGTCTGATGCAGGCT GGGAAGAATACTTTGATTACATCTTTCCAGAAGATGCTGCCAACCAACCTAACCTCAAACTCCTGGCCAT GGCCAAACTGTGGAAGAAACAGCAGCAGGAAAAGGAGGATGCTGAGCACCATCCAGATGAGGACGTCGAT GAGAGTGAATCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238911 representing NM_001278627 Red=Cloning site Green=Tags(s) MEMKNRQVNHARNIWDRAITTLPRVNQFWYKYTYMEEMLGNVAGARQVFERWMEWQPEEQAWHSYINFEL RYKEVDRARTIYERFVLVHPDVKNWIKYARFEEKHAYFAHARKVYERAVEFFGDEHMDEHLYVAFAKFEE NQKEFERVRVIYKYALDRISKQDAQELFKNYTIFEKKFGDRRGIEDIIVSKRRFQYEEEVKANPHNYDAW FDYLRLVESDAEAEAVREVYERAIANVPPIQEKRHWKRYIYLWINYALYEELEAKDPERTRQVYQASLEL IPHKKFTFAKMWILYAQFEIRQKNLSLARRALGTSIGKCPKNKLFKVYIELELQLREFDRCRKLYEKFLE FGPENCTSWIKFAELETILGDIDRARAIYELAISQPRLDMPEVLWKSYIDFEIEQEETERTRNLYRRLLQ RTQHVKVWISFAQFELSSGKEGSLTKCRQIYEEANKTMRNCEEKEERLMLLESWRSFEEEFGTASDKERV DKLMPEKVKKRRKVQTDDGSDAGWEEYFDYIFPEDAANQPNLKLLAMAKLWKKQQQEKEDAEHHPDEDVD ESES TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001278627
ORF Size	1692 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278627.1, NP_001265556.1
RefSeq Size	4100 bp
RefSeq ORF	1695 bp
Locus ID	51340
UniProt ID	Q9BZJ0
Protein Pathways	Spliceosome
MW	69.1 kDa
Gene Summary	The crooked neck (crn) gene of Drosophila is essential for embryogenesis and is thought to be involved in cell cycle progression and pre-mRNA splicing. A protein encoded by this human locus has been found to localize to pre-mRNA splicing complexes in the nucleus and is necessary for pre-mRNA splicing. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2013]

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