AAV Titer Calculator

The titer of AAV particles is a critical parameter, as it helps determine the optimal viral dose required for effective gene delivery. By inputting the relevant data into the AAV titer calculator, researchers can obtain an accurate titer estimation of their samples, aiding in experimental planning and optimizing gene delivery outcomes.

  1. Fill out the Ct values for the Standard Curve in the highlighted cells
  2. Enter your Sample Ct values in the "Unknown Virus Samples" section
  3. Titer is automatically calculated
  4. Adjust titer if you diluted virus sample for the titer assay, performed DNase I treatment, or viral lysis, eg. Titer x 100 (virus dil.) x 10 (DNaseI dil.) x 10 (lysis dil.)
STANDARD CURVE
Ct Duplicate1 Ct Duplicate2 Ct Average Dilution Titer (GC/ml)
SLOPE AND Y-INTERCEPT

y = m * ln(x) + b

UNKNOWN VIRUS SAMPLES
Sample Name Ct Duplicate1 Ct Duplicate2 Ct Average Titer (GC/ml)

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