BCL10 pSer138 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 6D3]

Specifications

Product Data
Clone Name	6D3
Applications	WB
Recommend Dilution	Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: BSA/Tween 20 based blocking and blot incubation buffer. Included Positive Control: Cell lysate from serum starved SW480 cells (please see: 'Protocols').
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Phosphopeptide conjugated to KLH
Specificity	This antibody specifically recognizes bcl-10 phosphorylated at Serine 138 at 32 kDa in Western blot.
Formulation	1 ml 2 x PBS / 0.09% Sodium Azide / PEG and Sucrose. State: Purified State: Lyophilized purified IgG fraction.
Reconstitution Method	Restore with 1 ml water (15 min, RT).
Purification	Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.
Conjugation	Unconjugated
Storage Condition	Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months. Avoid repeated freezing and thawing.
Gene Name	B-cell CLL/lymphoma 10
Database Link	Entrez Gene 8915 Human UniProt ID O95999
Background	Bcl-10 is an apoptosis-inducing molecule interacting with caspase 9. Bcl-10 enhances pro-caspase 9 processing and induces apoptosis through caspase 9 activation.
Synonyms	BCL10, CIPER, CLAP, cCARMEN, mE10, c-E10, hCLAP
Note	Molecular Weight: 32 kDa Protocol: Positive Control Cell Lysate: SW 480 Untreated Format: Lyophilized cell lysate from serum starved SW480 cells. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended for applications with native proteins such as in immunoprecipitation. Storage: Aliquote reconstituted product and store frozen. Avoid repeated fereezing and thawing.
Reference Data

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