N Cadherin (CDH2) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 7E9]

Specifications

Product Data
Clone Name	7E9
Applications	WB
Recommend Dilution	Immunoblotting: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Included Positive Control: Cell lysate from untreated SH-SY5Y cells (See Ptotocols for more details).
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Peptide conjugated to hemocyanin. Epitope: Cytoplasmic domain
Specificity	This antibody specifically recognizes N-Cadherin at 127 kDa.
Formulation	1 ml 2 x PBS / 0.09% Sodium Azide / PEG and Sucrose State: Purified State: Lyophilized purified IgG fraction.
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Subsequent Ultrafiltration and Size Exclusion Chromatography.
Conjugation	Unconjugated
Storage Condition	Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months. Avoid repeated freezing and thawing.
Gene Name	cadherin 2
Database Link	Entrez Gene 1000 Human UniProt ID P19022
Background	Cadherins are a family of transmembrane glycoproteins that play a key role in calcium-dependent cell-cell adhesion. Several members of the cadherin family have been identified so far, including E- (epithelial), P- (placental), N- (neuronal), and M- (muscle) cadherin.
Synonyms	CDH2, CDHN, NCAD, Neural cadherin, CDw325, Cadherin N, N-Cadherin
Note	Protocol: Positive Control: Cell lysate from untreated SH-SY5Y cells Format: Lyophilized cell lysate from serum starved SH-SY5Y cells. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended for applications with native proteins such as in immunoprecipitation. Storage: Aliquote reconstituted product and store frozen. Avoid repeated fereezing and thawing.
Reference Data

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