beta Catenin (CTNNB1) (exon 3) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 9G2]
CAT#: AM00020PU-N
beta Catenin (CTNNB1) (exon 3) (incl. pos. control) mouse monoclonal antibody, clone 9G2, Purified
Conjugation: Biotin
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CNY 5,148.00
货期*
5周
规格
Cited in 1 publication. |
Specifications
Product Data | |
Clone Name | 9G2 |
Applications | ELISA, IF, IHC, IP, WB |
Recommend Dilution | ELISA: 0.05 μg/ml. Western Blot: 0.5 μg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Immunoprecipitation: 1-10 μg per 106 vanadate treated A431 cells. Immunocytochemistry: 0.1-1 μg/ml. Immunohistochemistry on Paraffin Sections. Included Positive Control: Cell lysate from untreated SW480 cells (for details see "Protocols"). |
Reactivity | Canine, Human, Mouse |
Host | Mouse |
Clonality | Monoclonal |
Immunogen | Recombinant β-Catenin. Epitope: Exon 3/alpha-Catenin-binding site |
Specificity | This antibody specifically interacts with exon 3 (alpha-Catenin-binding site) of beta-Catenin. |
Formulation | 1 ml 2 x PBS containing 0.09% Sodium Azide, PEG and Sucrose State: Purified State: Lyophilized purified IgG fraction |
Reconstitution Method | Restore with 1 ml H2O (15 min, RT). |
Purification | Size Exclusion Chromatography |
Conjugation | Unconjugated |
Storage Condition | Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months. |
Predicted Protein Size | 90 kDa |
Gene Name | catenin beta 1 |
Database Link | |
Background | The α-, β- and γ-catenins are cytoplasmic proteins mediating the interaction of Ca2+-dependent transmembrane adhesion molecules (cadherins) with the cytoskeletal network. The direct interaction of β-catenin with the cytoplasmic domain of cadherins plays a crucial role for cell-cell adhesion and signal transmission between neighbouring cells. Recent studies indicate that β-catenin may also play a role in tumorigenesis since it forms complexes with the tumor suppressor gene product APC. β-catenin directly interacts and constitutively activates transcription factors of the TCF/LEF gene family. Thus it is proposed that β-catenin plays a dual role not only in the maintainance and regulation of cell-cell interactions but also in the regulation of gene activity. |
Synonyms | CTNNB1, CTNNB, Beta-catenin |
Note | Protocol: Positive Control: Cell lysate from untreated SW480 cells. Formulation: Lyophilized Cell Lysate from Serum starved SW480 cells. Stability: Reconstitute by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The Positive Control Cell Lysate is recommended for immunoblot applications. Use 20µl molecular weight marker per lane. Note: Use BSA based blot incubation buffers. Milk, Casein and Blotto might interfere with antibody - antigen interaction. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Shelf life: one year from despatch. |
Reference Data |
Citations (1)
The use of this Antibodies has been cited in the following citations: |
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Expression of steroid hormone receptors in the genital structures of a true hermaphrodite pug dog
,Bartel C, Meyer F, SchC$fer-Somi S, Walter I,
Reprod. Domest. Anim.
,PubMed ID 25472589
[CTNNB1]
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