beta Catenin (CTNNB1) (exon 3) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 9G2]

CAT#: AM00020PU-N

beta Catenin (CTNNB1) (exon 3) (incl. pos. control) mouse monoclonal antibody, clone 9G2, Purified

Conjugation: Unconjugated Biotin



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CNY 5,148.00


货期*
5周

规格
    • 100 ug

Cited in 1 publication.

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Specifications

Product Data
Clone Name 9G2
Applications ELISA, IF, IHC, IP, WB
Recommend Dilution ELISA: 0.05 μg/ml.
Western Blot: 0.5 μg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
Immunoprecipitation: 1-10 μg per 106 vanadate treated A431 cells.
Immunocytochemistry: 0.1-1 μg/ml.
Immunohistochemistry on Paraffin Sections.
Included Positive Control: Cell lysate from untreated SW480 cells (for details see "Protocols").
Reactivity Canine, Human, Mouse
Host Mouse
Clonality Monoclonal
Immunogen Recombinant β-Catenin.
Epitope: Exon 3/alpha-Catenin-binding site
Specificity This antibody specifically interacts with exon 3 (alpha-Catenin-binding site) of beta-Catenin.
Formulation 1 ml 2 x PBS containing 0.09% Sodium Azide, PEG and Sucrose
State: Purified
State: Lyophilized purified IgG fraction
Reconstitution Method Restore with 1 ml H2O (15 min, RT).
Purification Size Exclusion Chromatography
Conjugation Unconjugated
Storage Condition Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C.
Avoid repeated freezing and thawing.
Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Predicted Protein Size 90 kDa
Gene Name catenin beta 1
Background The α-, β- and γ-catenins are cytoplasmic proteins mediating the interaction of Ca2+-dependent transmembrane adhesion molecules (cadherins) with the cytoskeletal network. The direct interaction of β-catenin with the cytoplasmic domain of cadherins plays a crucial role for cell-cell adhesion and signal transmission between neighbouring cells. Recent studies indicate that β-catenin may also play a role in tumorigenesis since it forms complexes with the tumor suppressor gene product APC. β-catenin directly interacts and constitutively activates transcription factors of the TCF/LEF gene family. Thus it is proposed that β-catenin plays a dual role not only in the maintainance and regulation of cell-cell interactions but also in the regulation of gene activity.
Synonyms CTNNB1, CTNNB, Beta-catenin
Note Protocol: Positive Control: Cell lysate from untreated SW480 cells.

Formulation: Lyophilized Cell Lysate from Serum starved SW480 cells.

Stability:
Reconstitute by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.

Application:
The Positive Control Cell Lysate is recommended for immunoblot applications. Use 20µl molecular weight marker per lane. Note: Use BSA based blot incubation buffers. Milk, Casein and Blotto might interfere with antibody - antigen interaction.

Storage:
Aliquote and store frozen.
Avoid repeated freeze/thaw cycles.
Shelf life: one year from despatch.
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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