TPTEP2-CSNK1E (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 4D7]

CAT#: AM00025PU-N

TPTEP2-CSNK1E (incl. pos. control) mouse monoclonal antibody, clone 4D7, Purified



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CNY 5,973.00


货期*
5周

规格
    • 100 ug

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Specifications

Product Data
Clone Name 4D7
Applications ELISA, WB
Recommend Dilution ELISA: 0.1 µg/ml.
Western blot:  Use at 0.5 µg/ml for HRPO/ECL detection. Detects a band at 45kD.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
Included Positive Control: Cell lysate from Untreated A431 cells.
Reactivity Canine, Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Immunogen Peptide (aa 355 - 380 of CK1e) conjugated to hemocyanin
Specificity Mab CK1e-4D7 specifically recognizes the epsilon isoform of CK1.
Formulation 1ml 2xPBS containing 0.09% Sodium Azide, PEG and Sucrose
State: Purified
State: Lyophilized purified IgG fraction
Reconstitution Method Reconstitute with 1 ml H2O (15 min, RT)
Concentration lot specific
Purification Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
Conjugation Unconjugated
Storage Condition Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and
freeze in liquid nitrogen) at -80°C.
Avoid repeated freezing and thawing.
Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Gene Name TPTEP2-CSNK1E readthrough
Background Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as subtrates. It can also phosphorylate a large number of proteins and participates in Wnt signaling.
Synonyms CKI-epsilon, CKIe, CSNK1E
Note The lyophilized cell lysates (see below) contain SDS and are not recommended for applications with native proteins such as Immunoprecipitation.

Protocol: Positive Control: Cell lysate from Untreated A431 cells.

Format: Lyophilized cell lysate from Serum starved A431 cells

Reconstitution: Restore by addition of 200 μl H2O. After complete solubilization add 200 μl
2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. 
Aliquote and store frozen. Avoid repeated freeze/thaw cycles.

Application: The positive control cell lysate is recommended for immunoblot applications.
20 μl of positive control cell lysate correspond to ca. 20.000 cells.

Use 20 μl/lane (mini gel) for HRPO/ECL detection of the target proteins.

Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended for
applications with native proteins such as in immunoprecipitation.
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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