EGFR pSer1047 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 1H9]

Specifications

Product Data
Clone Name	1H9
Applications	ELISA, IF, IP, WB
Recommend Dilution	Western blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. ELISA: 0.1 µg/ml. Immunoprecipitation: 1-10 µg per 10e6 pervanadate-treated A431 cells. Immunocytochemistry: 0.1-1 µg/ml. Luminex. Included Positive Control: Cell lysate from pervanadate-treated HepG2 cells (See Protocols below).
Reactivity	Canine, Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Phosphopeptide conjugated to KLH.  Epitope: Phosphoserine 1047, RYSpSDST
Specificity	This antibody recognizes EGFR phosphorylated at Serine 1047.
Formulation	PBS containing 0.09% Sodium Azide, PEG and Sucrose State: Purified State: Lyophilized purified Ig fraction
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilised product upon arrival at -20°C. Following reconstitution aliquot and store at 2-8°C for up to three months or freeze in liquid nitrogen at -80°C for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate, we recommend centrifugation before use.
Predicted Protein Size	180 kDa
Gene Name	epidermal growth factor receptor
Database Link	Entrez Gene 1956 Human UniProt ID P00533
Background	EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y845 by src family kinases. Phosphorylation of Y1045 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1047 by CamKinase II leads to attenuation of kinase activity; phosphorylation of T654 (by PKC) and T669 (by MAPK, p38) interferes with receptor endocytosis/recycling.
Synonyms	Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1
Note	Protocol: Included Positive Control: Cell lysate from pervanadate-treated HepG2 cells. Format: Lyophilized cell lysate from HepG2 cells. Serum starved cells were treated for 15 min with pervanadate. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended for applications with native proteins such as in immunoprecipitation. Storage: Aliquote reconstituted product and store frozen. Avoid repeated freezing and thawing.
Reference Data

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